Trace and Ultratrace Analysis by HPLC

High Performance Liquid Chromatography in Biotechnology Edited By William S. Hancock Analytical chemists, biochemists, or chemical engineers will find this up-to-date guide to HPLC's recent developments essential to enhancing their on-the-job technical expertise. Extensive coverage includes the broad applications of HPLC, ranging from major chromatographic techniques (including reversed phase, ion exchange, affinity and hydrophobic interaction chromatography) to specific separations such as in monoclonal antibody and nucleic acid purification. The book also highlights the techniques required for a quality control program and such advanced technology as mass spectrometry. 1990 (0 471-82584-0) 576 pp. Unified Separation Science J. Calvin Giddings This advanced text/monograph brings together, for the first time in a single volume, the variety of techniques used for chemical separations by outlining their common underlying mechanisms. The mass transport phenomena underlying all separation processes are developed in a simple physicalmathematical form, facilitating analysis of alternative separation techniques and the factors integral to separation power. The first six chapters provide generic background material applicable to a wide range of separation methods, including the theoretical foundations of separations rooted in transport, flow, and equilibrium phenomena. The final five chapters illustrate specific techniques and methods, including electrophoretic and sedimentation techniques, field-flow fractionation, and chromatography. 1991 (0 471-52089-6) 352 pp. The Scope of Trace and Ultratrace Analysis by HPLC.

Theoretical Considerations.

Equipment Considerations.

Sensitive Detectors in HPLC.

Sample Preparation.

Method Development for Trace and Ultratrace Analysis.

Selectivity Optimization for Trace and Ultratrace Analysis.

Optimizing Detectability.

Applications.

Index. About the author SATINDER AHUJA is Senior Research Fellow in the Physical and Analytical Chemistry Division of CIBA-Geigy Corporation. Dr. Ahuja, who enjoys an international reputation in his field, is a member of the American Chemical Society, IUPAC and AAPS, and the editor/author of five books, including Ultratrace Analysis of Pharmaceuticals and Other Compounds of Interest (Wiley, 1986) and Selectivity and Detectability Optimizations in HPLC (Wiley, 1989). His publications and presentations include basic investigations on various modes of chromatography, automation/computerization, and analytical evaluations of drug candidates. Dr. Ahuja earned his PhD in pharmaceutical analytical chemistry at the Philadelphia College of Pharmacy and Science. Trace and ultratrace analyses have been integral to establishing a higher—and safer—quality of life. From cosmetics, pharmaceuticals, and fuel to the very food and water we consume, these ever-useful analytical tools have helped keep harmful carcinogens and other dangerous contaminants out of our lives. Of the various separation techniques used for trace and ultratrace analyses, high performance liquid chromatography is by far the most popular and commonly used. HPLC’s heightened capacity for analyzing and discovering new compounds has been essential to the success of trace and ultratrace analyses. This new monograph by a leading scientist provides the first definitive, technically up-to-date look at the theory, equipment, and applications of what is becoming chemistry’s most powerfully reliable analytical technique. Trace and Ultratrace Analysis by HPLC outlines in a clear, progressive format the state of the art of the technique, covering:

• The scope of trace and ultratrace analysis by HPLC
• Considerations for HPLC equipment
• Sensitive detectors in HPLC
• Sample preparation
• Method development for trace and ultratrace analysis
• Selectivity optimization for trace and ultratrace analysis
• Computer-based optimizations
• Optimizing detectability

In addition, the book provides an encyclopedic compendium of common substances requiring trace and ultratrace analysis—including analgesics, antibiotics, tranquilizers, and cosmetics as well as vitamins, fruits and vegetables, and beverages. Also provided is an intriguing glimpse into the projected uses of the technique as a key to understanding neural disorders such as Parkinson’s disease and schizophrenia. Combining clear exposition with rich technical detail, Trace and Ultratrace Analysis by HPLC provides an unparalleled and up-to-date look at one of chemistry’s most reliable analytical tools. High Performance Liquid Chromatography in Biotechnology Edited By William S. Hancock Analytical chemists, biochemists, or chemical engineers will find this up-to-date guide to HPLC’s recent developments essential to enhancing their on-the-job technical expertise. Extensive coverage includes the broad applications of HPLC, ranging from major chromatographic techniques (including reversed phase, ion exchange, affinity and hydrophobic interaction chromatography) to specific separations such as in monoclonal antibody and nucleic acid purification. The book also highlights the techniques required for a quality control program and such advanced technology as mass spectrometry. 1990 (0 471-82584-0) 576 pp. Unified Separation Science J. Calvin Giddings This advanced text/monograph brings together, for the first time in a single volume, the variety of techniques used for chemical separations by outlining their common underlying mechanisms. The mass transport phenomena underlying all separation processes are developed in a simple physicalmathematical form, facilitating analysis of alternative separation techniques and the factors integral to separation power. The first six chapters provide generic background material applicable to a wide range of separation methods, including the theoretical foundations of separations rooted in transport, flow, and equilibrium phenomena. The final five chapters illustrate specific techniques and methods, including electrophoretic and sedimentation techniques, field-flow fractionation, and chromatography. 1991 (0 471-52089-6) 352 pp.