{"product_id":"the-fundamentals-of-scientific-research-isbn-9781118867846","title":"The Fundamentals of Scientific Research","description":"\u003cp\u003e\u003ci\u003eThe Fundamentals of Scientific Research: An Introductory Laboratory Manual \u003c\/i\u003eis a laboratory manual geared towards first semester undergraduates enrolled in general biology courses focusing on cell biology. This laboratory curriculum centers on studying a single organism throughout the entire semester – \u003ci\u003eSerratia marcescens\u003c\/i\u003e, or \u003ci\u003eS. marcescens\u003c\/i\u003e, a bacterium unique in its production of the red pigment prodigiosin.\u003c\/p\u003e \u003cp\u003eThe manual separates the laboratory course into two separate modules. The first module familiarizes students with the organism and lab equipment by performing growth curves, Lowry protein assays, quantifying prodigiosin and ATP production, and by performing complementation studies to understand the biochemical pathway responsible for prodigiosin production. Students learn to use Microsoft Excel to prepare and present data in graphical format, and how to calculate their data into meaningful numbers that can be compared across experiments. The second module requires that the students employ UV mutagenesis to generate hyper-pigmented mutants of \u003ci\u003eS. marcescens\u003c\/i\u003e for further characterization. Students use experimental data and protocols learned in the first module to help them develop their own hypotheses, experimental protocols, and to analyze their own data.\u003c\/p\u003e \u003cp\u003eBefore each lab, students are required to answer questions designed to probe their understanding of required pre-laboratory reading materials. Questions also guide the students through the development of hypotheses and predictions. Following each laboratory, students then answer a series of post-laboratory questions to guide them through the presentation and analysis of their data, and how to place their data into the context of primary literature. Students are also asked to review their initial hypotheses and predictions to determine if their conclusions are supportive. A formal laboratory report is also to be completed after each module, in a format similar to that of primary scientific literature.\u003c\/p\u003e \u003cp\u003e\u003ci\u003eThe Fundamentals of Scientific Research: An Introductory Laboratory Manual \u003c\/i\u003eis an invaluable resource to undergraduates majoring in the life sciences.\u003c\/p\u003e \u003cp\u003ePreface xi\u003c\/p\u003e \u003cp\u003eAcknowledgments xv\u003c\/p\u003e \u003cp\u003eAbout the Companion Website xvii\u003c\/p\u003e \u003cp\u003eIntroduction xix\u003c\/p\u003e \u003cp\u003e\u003cb\u003eModule 1 Working with and Learning About Common Laboratory Techniques and Equipment 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eExercise 1A Using Common Laboratory Tools to Evaluate Measurements Pre-laboratory Thinking Questions 3\u003c\/p\u003e \u003cp\u003eExercise 1B Using Common Laboratory Tools to Evaluate Measurements 4\u003c\/p\u003e \u003cp\u003eExercise 1C Using Common Laboratory Tools to Evaluate Measurements Post-laboratory Thinking Questions 18\u003c\/p\u003e \u003cp\u003eExercise 2A Using Microscopy to Evaluate Cell Size and Complexity Pre-laboratory Thinking Questions 19\u003c\/p\u003e \u003cp\u003eExercise 2B Using Microscopy to Evaluate Cell Size and Complexity 20\u003c\/p\u003e \u003cp\u003eExercise 2C Using Microscopy to Evaluate Cell Size and Complexity Post-laboratory Thinking Questions 32\u003c\/p\u003e \u003cp\u003eExercise 3A The Bacterial Growth Curve Pre-laboratory Thinking Questions 35\u003c\/p\u003e \u003cp\u003eExercise 3B The Bacterial Growth Curve 36\u003c\/p\u003e \u003cp\u003eExercise 3C The Bacterial Growth Curve Post-laboratory Thinking Questions 55\u003c\/p\u003e \u003cp\u003e\u003cb\u003eModule 2 Working with and Learning About Serratia marcescens in the Laboratory 57\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eExercise 4A Protein Concentration Versus Growth Stage Pre-laboratory Thinking Questions 59\u003c\/p\u003e \u003cp\u003eExercise 4B Protein Concentration Versus Growth Stage 60\u003c\/p\u003e \u003cp\u003eExercise 4C Protein Concentration Versus Growth Stage Post-laboratory Thinking Questions 69\u003c\/p\u003e \u003cp\u003eExercise 5A Measuring Prodigiosin Pre-laboratory Thinking Questions 71\u003c\/p\u003e \u003cp\u003eExercise 5B Measuring Prodigiosin 72\u003c\/p\u003e \u003cp\u003eExercise 5C Measuring Prodigiosin Post-laboratory Thinking Questions 84\u003c\/p\u003e \u003cp\u003eExercise 6A Conditions Affecting the Growth of and Prodigiosin Production by Serratia marcescens Pre-laboratory Thinking Questions 87\u003c\/p\u003e \u003cp\u003eExercise 6B Conditions Affecting the Growth of and Prodigiosin Production by S. marcescens 91\u003c\/p\u003e \u003cp\u003eExercise 6C Formal Laboratory Report Describing the Conditions Affecting the Growth of and Prodigiosin Production by S. marcescens 95\u003c\/p\u003e \u003cp\u003eExercise 7A Biochemistry of Prodigiosin Production Pre-laboratory Thinking Questions 101\u003c\/p\u003e \u003cp\u003eExercise 7B Biochemistry of Prodigiosin Production 102\u003c\/p\u003e \u003cp\u003eExercise 7C Biochemistry of Prodigiosin Production Post-laboratory Thinking Questions 112\u003c\/p\u003e \u003cp\u003eExercise 8A The Probability Basis for Mutation Rate Calculation: A Dice]Roll Exercise Pre-laboratory Thinking Questions 113\u003c\/p\u003e \u003cp\u003eExercise 8B The Probability Basis for Mutation Rate Calculation: A Dice]Roll Exercise 114\u003c\/p\u003e \u003cp\u003eExercise 8C The Probability Basis for Mutation Rate Calculation: A Dice]Roll Exercise Post-laboratory Thinking Questions 120\u003c\/p\u003e \u003cp\u003eExercise 9A Understanding Evolution by the Generation of UV Light]Induced Prodigiosin Mutants Pre-laboratory Thinking Questions 121\u003c\/p\u003e \u003cp\u003eExercise 9B Understanding Evolution by the Generation of UV Light]Induced Prodigiosin Mutants 122\u003c\/p\u003e \u003cp\u003eExercise 9C Understanding Evolution by the Generation of UV Light]Induced Prodigiosin Mutants Post-laboratory Thinking Questions 131\u003c\/p\u003e \u003cp\u003eExercise 10A Understanding the Energy Spilling Properties of Prodigiosin Pre-laboratory Thinking Questions 137\u003c\/p\u003e \u003cp\u003eExercise 10B Understanding the Energy Spilling Properties of Prodigiosin 139\u003c\/p\u003e \u003cp\u003eExercise 10C Understanding the Energy Spilling Properties of Prodigiosin Post-laboratory Thinking Questions 146\u003c\/p\u003e \u003cp\u003e\u003cb\u003eModule 3 Initial Characterization of Novel Serratia marcescens Prodigiosin Mutants 147\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eExercise 11A Prodigiosin Mutant Study Part 1 Pre-laboratory Thinking Questions 149\u003c\/p\u003e \u003cp\u003eExercise 11B Prodigiosin Mutant Study Part 1 150\u003c\/p\u003e \u003cp\u003eExercise 11C Prodigiosin Mutant Study Part 1 Post-laboratory Thinking Questions 153\u003c\/p\u003e \u003cp\u003eExercise 12A Prodigiosin Mutant Study Part 2 Pre-laboratory Thinking Questions 155\u003c\/p\u003e \u003cp\u003eExercise 12B Prodigiosin Mutant Study Part 2 156\u003c\/p\u003e \u003cp\u003eExercise 12C Formal Laboratory Report 2: Prodigiosin Mutant Study 158\u003c\/p\u003e \u003cp\u003eAppendix A CSE Citation and Reference List Format Guidelines 163\u003c\/p\u003e \u003cp\u003eAppendix B Prodigiosin Biosynthesis 165\u003c\/p\u003e \u003cp\u003eReferences 167\u003c\/p\u003e \u003cp\u003eIndex 169\u003c\/p\u003e \u003cp\u003e\u003cb\u003eMarcy A. Kelly\u003c\/b\u003e is Professor and Assistant Chair of the Department of Biology at Pace University in New York, NY, USA.\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePryce L. Haddix\u003c\/b\u003e is an Associate Professor of Biology at Auburn University in Montgomery, AL, USA.\u003c\/p\u003e \u003cp\u003e\u003ci\u003eThe Fundamentals of Scientific Research: An Introductory Laboratory Manual\u003c\/i\u003e is a laboratory manual geared toward first-semester undergraduates enrolled in general biology courses focusing on cell biology. This laboratory curriculum centers on studying a single organism throughout the entire semester—Serratia marcescens or S. marcescens—a bacterium unique in its production of the red pigment prodigiosin.\u003c\/p\u003e \u003cp\u003eThe manual separates the laboratory course into three separate modules. The first two modules familiarizes students with the organism and lab equipment by performing growth curves, Lowry protein assays, quantifying prodigiosin and ATP production, and by performing complementation studies to understand the biochemical pathways responsible for prodigiosin production. Students learn how to use Microsoft Excel to prepare and present data in graphical format and how to calculate their data into meaningful numbers that can be compared across experiments. The third module requires that the students employ UV mutagenesis to generate hyper-pigmented mutants of S. marcescens for further characterization. Students use experimental data and protocols learned in the first module to help them develop their own hypotheses and experimental protocols, and to analyze their own data.\u003c\/p\u003e \u003cp\u003eBefore each laboratory, students are required to answer questions designed to probe their understanding of required pre-laboratory reading materials. Questions also guide the students through the development of hypotheses and predictions. Following each laboratory, students then answer a series of post-laboratory questions to guide them through the presentation and analysis of their data, and how to place their data into the context of primary literature. Students are also asked to review their initial hypotheses and predictions to determine if their conclusions are supportive. A formal laboratory report is also to be completed after each module, in a format similar to that of primary scientific literature.\u003c\/p\u003e \u003cp\u003e\u003ci\u003eThe Fundamentals of Scientific Research: An Introductory Laboratory Manual\u003c\/i\u003e is an invaluable resource to undergraduates majoring in the life sciences.\u003c\/p\u003e \u003cul\u003e \u003cli\u003eText focuses on one organism, S. marcescens, throughout the semester with a focused goal: to enhance the organism’s production of prodigiosin\u003c\/li\u003e \u003cli\u003eTeaches students how to use laboratory equipment correctly and effectively\u003c\/li\u003e \u003cli\u003eFollows structure of laboratory curriculum similar to professional research environment\u003c\/li\u003e \u003cli\u003ePrompts first-year undergraduates to read, evaluate, and use their own data in the context of primary scientific literature\u003c\/li\u003e \u003c\/ul\u003e","brand":"Wiley-Blackwell","offers":[{"title":"Default Title","offer_id":47990233497829,"sku":"NP9781118867846","price":55.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9781118867846.jpg?v=1761787010","url":"https:\/\/k12savings.com\/products\/the-fundamentals-of-scientific-research-isbn-9781118867846","provider":"K12savings","version":"1.0","type":"link"}