{"product_id":"sample-preparation-in-lc-ms-bioanalysis-isbn-9781119274292","title":"Sample Preparation in LC-MS Bioanalysis","description":"\u003cp\u003e\u003cb\u003eRevised and Expanded Handbook Provides Comprehensive Introduction and Complete Instruction for Sample Preparation in Vital Category of Bioanalysis\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eFollowing in the footsteps of the previously published \u003ci\u003eHandbook of LC-MS Bioanalysis\u003c\/i\u003e, this book is a thorough and timely guide to all important sample preparation techniques used for quantitative Liquid Chromatography–Mass Spectrometry (LC-MS) bioanalysis of small and large molecules. LC-MS bioanalysis is a key element of pharmaceutical research and development, post-approval therapeutic drug monitoring, and many other studies used in human healthcare. \u003c\/p\u003e \u003cp\u003eWhile advances are continually being made in key aspects of LC-MS bioanalysis such as sensitivity and throughput, the value of research\/study mentioned above is still heavily dependent on the availability of high-quality data, for which sample preparation plays the critical role. Thus, this text provides researchers in industry, academia, and regulatory agencies with detailed sample preparation techniques and step-by-step protocols on proper extraction of various analyte(s) of interest from biological samples for LC-MS quantification, in accordance with current health authority regulations and industry best practices. The three sections of the book with a total of 26 chapters cover topics that include:\u003c\/p\u003e \u003cul\u003e \u003cli\u003eCurrent basic sample preparation techniques (e.g., protein precipitation, liquid-liquid extraction, solid-phase extraction, salting-out assisted liquid-liquid extraction, ultracentrifugation and ultrafiltration, microsampling, sample extraction via electromembranes)\u003c\/li\u003e \u003cli\u003eSample preparation techniques for uncommon biological matrices (e.g., tissues, hair, skin, nails, bones, mononuclear cells, cerebrospinal fluid, aqueous humor)\u003c\/li\u003e \u003cli\u003eCrucial aspects of LC-MS bioanalytical method development (e.g., pre-analytical considerations, derivation strategies, stability, non-specific binding) in addition to sample preparation techniques for challenging molecules (e.g., lipids, peptides, proteins, oligonucleotides, antibody-drug conjugates)\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003ci\u003eSample Preparation in LC-MS Bioanalysis\u003c\/i\u003e will prove a practical and highly valuable addition to the reference shelves of scientists and related professionals in a variety of fields, including pharmaceutical and biomedical research, mass spectrometry, and analytical chemistry, as well as practitioners in clinical pharmacology, toxicology, and therapeutic drug monitoring.\u003c\/p\u003e \u003cp\u003eList of Contributors xvi\u003c\/p\u003e \u003cp\u003ePreface xx\u003c\/p\u003e \u003cp\u003eList of Abbreviations xxii\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart I Current Sample Preparation Techniques in LC‐MS Bioanalysis 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Basic Sample Preparation Techniques in LC‐MS Bioanalysis: Protein Precipitation, Liquid–Liquid Extraction, and Solid‐Phase Extraction 3\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eWenkui Li, Wenying Jian, and Yunlin Fu\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e1.1 Introduction 3\u003c\/p\u003e \u003cp\u003e1.2 Physicochemical Properties of Drugs and Their Metabolites 4\u003c\/p\u003e \u003cp\u003e1.3 Pre‐analytical Variables of Analyte(s) of Interest in Biological Matrix 5\u003c\/p\u003e \u003cp\u003e1.4 Most Commonly Used Sample Preparation Methods in LC‐MS Bioanalysis 7\u003c\/p\u003e \u003cp\u003eReferences 27\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Online Extraction and Column Switching Techniques in LC‐MS Bioanalysis 31\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eYan Mao and Mike (Qingtao) Huang\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2.1 Introduction 31\u003c\/p\u003e \u003cp\u003e2.2 System Configuration 32\u003c\/p\u003e \u003cp\u003e2.3 Commonly Used Online Extraction Techniques 33\u003c\/p\u003e \u003cp\u003e2.4 Considerations for Utilizing Online Extraction Techniques 40\u003c\/p\u003e \u003cp\u003e2.5 Summary 41\u003c\/p\u003e \u003cp\u003eReferences 42\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Equilibrium Dialysis, Ultracentrifugation, and Ultrafiltration in LC‐MS Bioanalysis 45\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eTheo de Boer and Henri Meijering\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introduction 45\u003c\/p\u003e \u003cp\u003e3.2 Challenges and Considerations 46\u003c\/p\u003e \u003cp\u003e3.3 Experimental Procedures 46\u003c\/p\u003e \u003cp\u003e3.4 Summary 49\u003c\/p\u003e \u003cp\u003eReferences 51\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Phospholipid Depletion Techniques in LC‐MS Bioanalysis 52\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eStacy Brown and Jennifer Carmical\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e4.1 Introduction 52\u003c\/p\u003e \u003cp\u003e4.2 Impact of Phospholipids on Bioanalytical Methods 52\u003c\/p\u003e \u003cp\u003e4.3 Investigating Matrix Effects Associated with Phospholipids 55\u003c\/p\u003e \u003cp\u003e4.4 Minimizing Matrix Effects Associated with Phospholipids 56\u003c\/p\u003e \u003cp\u003e4.5 Removing Phospholipids Prior to LC‐MS Analysis 57\u003c\/p\u003e \u003cp\u003e4.6 Example Methods that Demonstrate Successful Phospholipid Removal 60\u003c\/p\u003e \u003cp\u003e4.7 Conclusions 63\u003c\/p\u003e \u003cp\u003eAcknowledgement 64\u003c\/p\u003e \u003cp\u003eReferences 64\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Salting‐out Assisted Liquid–Liquid Extraction (SALLE) in LC‐MS Bioanalysis 68\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eJun Zhang and Xin Xiong\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction 68\u003c\/p\u003e \u003cp\u003e5.2 Considerations in Developing a SALLE Method 68\u003c\/p\u003e \u003cp\u003e5.3 Combination of SALLE with Other Extraction Techniques 72\u003c\/p\u003e \u003cp\u003e5.4 Matrix Effect in SALLE 72\u003c\/p\u003e \u003cp\u003e5.5 Miniaturization and Automatization 73\u003c\/p\u003e \u003cp\u003e5.6 Summary 73\u003c\/p\u003e \u003cp\u003eReferences 73\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Supported Liquid Extraction (SLE) in LC‐MS Bioanalysis 76\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eZhongzhe Cheng and Hongliang Jiang\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction 76\u003c\/p\u003e \u003cp\u003e6.2 Principle of SLE 76\u003c\/p\u003e \u003cp\u003e6.3 Advantages and Limitation of SLE in Quantitative LC‐MS Bioanalysis 77\u003c\/p\u003e \u003cp\u003e6.4 Key Consideration in Developing Robust SLE‐LC‐MS Bioanalytical Method 79\u003c\/p\u003e \u003cp\u003e6.5 Representative Protocols 80\u003c\/p\u003e \u003cp\u003e6.6 Summary 81\u003c\/p\u003e \u003cp\u003eReferences 82\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Immunocapture in LC‐MS Bioanalysis 85\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eAng Liu\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction 85\u003c\/p\u003e \u003cp\u003e7.2 Experimental Workflow and Optimization 85\u003c\/p\u003e \u003cp\u003e7.3 Considerations on the Selection of Capture Reagents and the Limitations 86\u003c\/p\u003e \u003cp\u003e7.4 Platforms for Immunocapture 89\u003c\/p\u003e \u003cp\u003e7.5 Internal Standard Selection 89\u003c\/p\u003e \u003cp\u003e7.6 Performance Evaluation 90\u003c\/p\u003e \u003cp\u003e7.7 Applications and Representative Protocols 91\u003c\/p\u003e \u003cp\u003e7.8 Validation Criteria and Regulatory Considerations 94\u003c\/p\u003e \u003cp\u003e7.9 Summary 95\u003c\/p\u003e \u003cp\u003eReferences 95\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Microextraction Techniques in LC‐MS Bioanalysis 98\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eMarcio Rodrigues, Ana Fortuna, Amilcar Falcao, and Gilberto Alves\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction 98\u003c\/p\u003e \u003cp\u003e8.2 Solid‐Phase Microextraction 99\u003c\/p\u003e \u003cp\u003e8.3 Liquid‐Phase Microextraction 108\u003c\/p\u003e \u003cp\u003e8.4 Summary 113\u003c\/p\u003e \u003cp\u003eAcknowledgements 113\u003c\/p\u003e \u003cp\u003eReferences 113\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Microsampling Applications with LC‐MS Bioanalysis 117\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eChester L. Bowen and Matthew Barfield\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction 117\u003c\/p\u003e \u003cp\u003e9.2 Plasma Microsampling Considerations 118\u003c\/p\u003e \u003cp\u003e9.3 Dried Blood (Matrix) Spot (DBS) Considerations 121\u003c\/p\u003e \u003cp\u003e9.4 Volumetric Absorptive Microsampling (VAMS) 123\u003c\/p\u003e \u003cp\u003e9.5 Emerging Techniques 125\u003c\/p\u003e \u003cp\u003e9.6 Summary 126\u003c\/p\u003e \u003cp\u003eAcknowledgements 126\u003c\/p\u003e \u003cp\u003eReferences 126\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Nanomaterials for Sample Preparation in LC‐MS Bioanalysis 128\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eRodrigo A. Gonzalez‐Fuenzalida, Neus Jornet‐Martinez, Rosa Herraez‐Hernandez, and Pilar Campins‐Falco\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e10.1 Introduction 128\u003c\/p\u003e \u003cp\u003e10.2 Carbon Nanomaterials 128\u003c\/p\u003e \u003cp\u003e10.3 Metallic NPs 133\u003c\/p\u003e \u003cp\u003e10.4 Nanoporous Materials 135\u003c\/p\u003e \u003cp\u003e10.5 Future Perspectives 136\u003c\/p\u003e \u003cp\u003eAcknowledgements 136\u003c\/p\u003e \u003cp\u003eReferences 137\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Sample Preparation via Molecularly Imprinted Polymers (MIPs) in LC‐MS Bioanalysis 139\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eMyriam Diaz‐Alvarez and Antonio Martin‐Esteban\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e11.1 Introduction 139\u003c\/p\u003e \u003cp\u003e11.2 Preparation of MIPs 139\u003c\/p\u003e \u003cp\u003e11.3 MIPs for Sample Preparation in Bioanalysis 142\u003c\/p\u003e \u003cp\u003e11.4 Fragment Imprinting 147\u003c\/p\u003e \u003cp\u003e11.5 Summary 147\u003c\/p\u003e \u003cp\u003eReferences 147\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Stir‐bar Sorptive Extraction for Sample Preparation in LC‐MS Bioanalysis 152\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eMaria Kechagia, Maria Kissoudi, and Victoria F. Samanidou\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction 152\u003c\/p\u003e \u003cp\u003e12.2 SBSE Principle 153\u003c\/p\u003e \u003cp\u003e12.3 SBSE Steps 154\u003c\/p\u003e \u003cp\u003e12.4 Derivatization 155\u003c\/p\u003e \u003cp\u003e12.5 Coating Materials 156\u003c\/p\u003e \u003cp\u003e12.6 Applications 158\u003c\/p\u003e \u003cp\u003e12.7 Summary 161\u003c\/p\u003e \u003cp\u003eReferences 161\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Monolithic Spin Column Extraction in LC‐MS Bioanalysis 163\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eAkira Namera and Takeshi Saito\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e13.1 Introduction 163\u003c\/p\u003e \u003cp\u003e13.2 History of Monoliths 163\u003c\/p\u003e \u003cp\u003e13.3 The Use of Monolith as Sorbent in Solid‐Phase Extraction 164\u003c\/p\u003e \u003cp\u003e13.4 Monolithic Spin Column for Sample Preparation 165\u003c\/p\u003e \u003cp\u003eReferences 171\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 Aptamer‐based Sample Preparation in LC‐MS Bioanalysis 174\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eFuyou Du, Zongyu Wei, Qiulian Zeng, and Guihua Ruan\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e14.1 Introduction 174\u003c\/p\u003e \u003cp\u003e14.2 Aptamer‐based Sample Preparation 175\u003c\/p\u003e \u003cp\u003e14.3 Representative Protocols 181\u003c\/p\u003e \u003cp\u003e14.4 Summary 185\u003c\/p\u003e \u003cp\u003eAcknowledgements 185\u003c\/p\u003e \u003cp\u003eReferences 185\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 Sample Extraction via Electromembrane in LC‐MS Bioanalysis 188\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eMaria Ramos‐Payan, Rut Fernandez‐Torres, and Miguel Angel Bello‐Lopez\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e15.1 Introduction 188\u003c\/p\u003e \u003cp\u003e15.2 Factors Affecting the Extraction Efficiency of EME 190\u003c\/p\u003e \u003cp\u003e15.3 Recent Developments in EME 191\u003c\/p\u003e \u003cp\u003e15.4 Bioanalytical Applications 193\u003c\/p\u003e \u003cp\u003e15.5 Summary 197\u003c\/p\u003e \u003cp\u003eReferences 197\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart II Matrix‐specific Sample Preparation Techniques in LC‐MS Bioanalysis 201\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e16 Tissue Sample Preparation in LC‐MS Bioanalysis 203\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eHong Gao and John Williams\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e16.1 Introduction 203\u003c\/p\u003e \u003cp\u003e16.2 Selection of Homogenization Method 203\u003c\/p\u003e \u003cp\u003e16.3 Common Protocols 209\u003c\/p\u003e \u003cp\u003e16.4 Protocols for Special Tissue Sample Preparation 210\u003c\/p\u003e \u003cp\u003e16.5 Challenges Associated with Tissue Homogenization 212\u003c\/p\u003e \u003cp\u003e16.6 Summary 214\u003c\/p\u003e \u003cp\u003eReferences 214\u003c\/p\u003e \u003cp\u003e\u003cb\u003e17 Sample Preparation for LC‐MS Bioanalysis of Peripheral Blood Mononuclear Cells 217\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eYunlin Fu and Wenkui Li\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e17.1 Introduction 217\u003c\/p\u003e \u003cp\u003e17.2 Peripheral Blood Mononuclear Cells (PBMCs) 217\u003c\/p\u003e \u003cp\u003e17.3 Sample Preparation Workflow for LC‐MS Bioanalysis of PBMC Samples 217\u003c\/p\u003e \u003cp\u003e17.4 Representative Protocols 220\u003c\/p\u003e \u003cp\u003e17.5 Summary 222\u003c\/p\u003e \u003cp\u003eReferences 222\u003c\/p\u003e \u003cp\u003e\u003cb\u003e18 Sample Preparation for LC‐MS Bioanalysis of Urine, Cerebrospinal Fluid, Synovial Fluid, Sweat, Tears, and Aqueous Humor Samples 225\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eAllena J. Ji\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e18.1 Introduction 225\u003c\/p\u003e \u003cp\u003e18.2 Sample Preparation Methods for Urine 225\u003c\/p\u003e \u003cp\u003e18.3 Sample Preparation Methods for Cerebrospinal Fluid 230\u003c\/p\u003e \u003cp\u003e18.4 Sample Preparation Methods for Synovial Fluid 231\u003c\/p\u003e \u003cp\u003e18.5 Sample Preparation Methods for Sweat 231\u003c\/p\u003e \u003cp\u003e18.6 Sample Preparation Methods for Tears 234\u003c\/p\u003e \u003cp\u003e18.7 Sample Preparation Methods for Aqueous Humor 236\u003c\/p\u003e \u003cp\u003e18.8 Summary 236\u003c\/p\u003e \u003cp\u003eReferences 236\u003c\/p\u003e \u003cp\u003e\u003cb\u003e19 Sample Preparation for LC‐MS Bioanalysis of Liposomal Samples 238\u003c\/b\u003e\u003ci\u003e\u003cbr\u003e Wenying Jian, Rao N.V.S. Mamidi, and Naidong Weng\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e19.1 Introduction 238\u003c\/p\u003e \u003cp\u003e19.2 Major Types of Sample Extraction Techniques for Liposomal Samples 239\u003c\/p\u003e \u003cp\u003e19.3 Key Considerations in Sample Preparation for Liposomal Samples 242\u003c\/p\u003e \u003cp\u003e19.4 Typical Protocols 245\u003c\/p\u003e \u003cp\u003e19.5 Summary 246\u003c\/p\u003e \u003cp\u003eReferences 246\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart III Sample Preparation Techniques for LC‐MS Bioanalysis of Challenging Molecules 249\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e20 Key Pre‐analytical Considerations in LC‐MS Bioanalysis 251\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eFumin Li, Bruce J. Hidy, and Rand G. Jenkins\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e20.1 Introduction 251\u003c\/p\u003e \u003cp\u003e20.2 The Pre‐analytical Phase 251\u003c\/p\u003e \u003cp\u003e20.3 Bioanalytical Evaluation‐planning 253\u003c\/p\u003e \u003cp\u003e20.4 Common Pre‐analytical Issues in LC‐MS Bioanalysis 255\u003c\/p\u003e \u003cp\u003e20.5 Summary 258\u003c\/p\u003e \u003cp\u003eReferences 259\u003c\/p\u003e \u003cp\u003e\u003cb\u003e21 Derivatization in Sample Preparation for LC‐MS Bioanalysis 260\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eDafang Zhong and Yunting Zhu\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e21.1 Introduction 260\u003c\/p\u003e \u003cp\u003e21.2 Derivatization Strategies 260\u003c\/p\u003e \u003cp\u003e21.3 Key Considerations for Derivatization 264\u003c\/p\u003e \u003cp\u003e21.4 Application of Derivatization for Quantitative LC‐MS Bioanalysis 265\u003c\/p\u003e \u003cp\u003e21.5 Summary 270\u003c\/p\u003e \u003cp\u003eReferences 271\u003c\/p\u003e \u003cp\u003e\u003cb\u003e22 Sample Preparation for LC‐MS Bioanalysis of Lipids 275\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eSamuel Hofbauer, Ashkan Salamatipour, Ian A. Blair, and Clementina Mesaros\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e22.1 Introduction 275\u003c\/p\u003e \u003cp\u003e22.2 Sample Preparation for LC‐MS Bioanalysis of Lipids 275\u003c\/p\u003e \u003cp\u003e22.3 Case Studies of LC‐MS Bioanalysis of Lipids 280\u003c\/p\u003e \u003cp\u003e22.4 Summary 281\u003c\/p\u003e \u003cp\u003eReferences 282\u003c\/p\u003e \u003cp\u003e\u003cb\u003e23 Sample Preparation for LC‐MS Bioanalysis of Peptides 284\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eLong Yuan\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e23.1 Introduction 284\u003c\/p\u003e \u003cp\u003e23.2 Properties of Peptides and Sample Pretreatment 285\u003c\/p\u003e \u003cp\u003e23.3 Sample Preparation Strategies 287\u003c\/p\u003e \u003cp\u003e23.4 Conclusions 296\u003c\/p\u003e \u003cp\u003eAcknowledgements 297\u003c\/p\u003e \u003cp\u003eReferences 297\u003c\/p\u003e \u003cp\u003e\u003cb\u003e24 Sample Preparation for LC‐MS Bioanalysis of Proteins 304\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eNico van de Merbel\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e24.1 Introduction 304\u003c\/p\u003e \u003cp\u003e24.2 Intact Versus Digested Protein Analysis 305\u003c\/p\u003e \u003cp\u003e24.3 Enzymatic Digestion 306\u003c\/p\u003e \u003cp\u003e24.4 Protein Depletion 308\u003c\/p\u003e \u003cp\u003e24.5 Protein Extraction (Before Digestion) 308\u003c\/p\u003e \u003cp\u003e24.6 Peptide Extraction (After Digestion) 312\u003c\/p\u003e \u003cp\u003e24.7 Combined Protein and Peptide Extraction 314\u003c\/p\u003e \u003cp\u003e24.8 Summary 314\u003c\/p\u003e \u003cp\u003eReferences 315\u003c\/p\u003e \u003cp\u003e\u003cb\u003e25 Sample Preparation for LC‐MS Bioanalysis of Oligonucleotides 319\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eMichael G. Bartlett, Jaeah Kim, Babak Basiri, and Ning Li\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e25.1 Introduction 319\u003c\/p\u003e \u003cp\u003e25.2 Properties of Oligonucleotides and Associated Challenges in LC‐MS Bioanalysis 319\u003c\/p\u003e \u003cp\u003e25.3 Classes of Oligonucleotides 322\u003c\/p\u003e \u003cp\u003e25.4 Major Types of Sample Extraction Techniques 323\u003c\/p\u003e \u003cp\u003e25.5 Key Considerations in Sample Preparation for LC‐MS Bioanalysis of Oligonucleotides 327\u003c\/p\u003e \u003cp\u003e25.6 Representative Protocols 328\u003c\/p\u003e \u003cp\u003e25.7 Summary 330\u003c\/p\u003e \u003cp\u003eReferences 330\u003c\/p\u003e \u003cp\u003e\u003cb\u003e26 Sample Preparation for LC‐MS Bioanalysis of Antibody–Drug Conjugates 335\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eCong Wei and Ragu Ramanathan\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e26.1 Introduction 335\u003c\/p\u003e \u003cp\u003e26.2 Properties of ADC and Challenges for Sample Preparation 335\u003c\/p\u003e \u003cp\u003e26.3 Sample Preparation Methods and Common Protocols 342\u003c\/p\u003e \u003cp\u003e26.4 Future Perspective 347\u003c\/p\u003e \u003cp\u003eAcknowledgements 348\u003c\/p\u003e \u003cp\u003eReferences 348\u003c\/p\u003e \u003cp\u003eIndex 351\u003c\/p\u003e \"A welcome reference which provides a complete overview of current and new approaches in sample preparation for LC-MS...To have an exhaustive compilation of strategies with “practical tips and tricks” can only be inspiring for the readers helping him to make the best choice for his specific challenge.\" Gérard Hopfgartner, Analytical and Bioanalytical Chemistry, January 2020   \u003cp\u003e\u003cb\u003eWENKUI LI\u003c\/b\u003e is a Senior Fellow at the Novartis Institutes for BioMedical Research Pharmacokinetic Sciences. He serves on the Editorial Board of Biomedical Chromatography. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eWENYING JIAN\u003c\/b\u003e is a Senior Principal Scientist in Janssen Research and Development at the Johnson \u0026amp; Johnson Company. She serves on the Editorial Board of Journal of Pharmacological and Toxicological Methods. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eYUNLIN FU\u003c\/b\u003e is an Investigator at the Novartis Institutes for BioMedical Research Pharmacokinetic Sciences.    \u003c\/p\u003e\u003cp\u003e\u003cb\u003eRevised and expanded handbook provides comprehensive introduction and complete instruction for sample preparation in vital category of bioanalysis\u003c\/b\u003e \u003c\/p\u003e\u003cp\u003eFollowing in the footsteps of the previously published \u003ci\u003eHandbook of LC-MS Bioanalysis,\u003c\/i\u003e this book is a thorough and timely guide to all important sample preparation techniques used for quantitative Liquid Chromatography–Mass Spectrometry (LC-MS) bioanalysis of small and large molecules. LC-MS bioanalysis is a key element of pharmaceutical research and development, post-approval therapeutic drug monitoring, and many other studies in human healthcare. \u003c\/p\u003e\u003cp\u003eWhile advances are continually being made in key aspects of LC-MS bioanalysis such as sensitivity and throughput, the value of research\/study mentioned above is still heavily dependent on the availability of high-quality data, for which sample preparation plays the critical role. Thus, this text provides researchers in industry, academia, and regulatory agencies with detailed sample preparation techniques and step-by-step protocols on proper extraction of various analyte(s) of interest from biological samples for LC-MS quantification, in accordance with current health authority regulations and industry best practices. The three sections of the book with a total of 26 chapters cover topics that include: \u003c\/p\u003e\u003cul\u003e \u003cli\u003eCurrent basic sample preparation techniques (e.g., protein precipitation, liquid-liquid extraction, solid-phase extraction, salting-out assisted liquid-liquid extraction, ultracentrifugation and ultrafiltration, supported liquid extraction, and sample extraction via electromembranes)\u003c\/li\u003e \u003cli\u003eSample preparation techniques for uncommon biological matrices (e.g., tissues, hair, skin, nails, bones, mononuclear cells, cerebrospinal fluid, aqueous humor)\u003c\/li\u003e \u003cli\u003eCrucial aspects of LC-MS bioanalytical method development (e.g., pre-analytical considerations, derivatization strategies, stability, non-specific binding) in addition to sample preparation techniques for challenging molecules (e.g., lipids, peptides, proteins, oligonucleotides, antibody-drug conjugates)\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003ci\u003eSample Preparation in LC-MS Bioanalysis\u003c\/i\u003e will prove a practical and highly valuable addition to the reference shelves of scientists and related professionals in a variety of fields, including pharmaceutical and biomedical research, mass spectrometry, and analytical chemistry, as well as practitioners in clinical pharmacology, toxicology, and therapeutic drug monitoring.\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47989986296037,"sku":"NP9781119274292","price":180.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9781119274292.jpg?v=1761786112","url":"https:\/\/k12savings.com\/products\/sample-preparation-in-lc-ms-bioanalysis-isbn-9781119274292","provider":"K12savings","version":"1.0","type":"link"}