{"product_id":"reverse-genetics-of-rna-viruses-isbn-9780470979655","title":"Reverse Genetics of RNA Viruses","description":"\u003cp\u003eReverse genetics, the genetic manipulation of RNA viruses to create a wild-type or modified virus, has led to important advances in our understanding of viral gene function and interaction with host cells. Since many severe viral human and animal pathogens are RNA viruses, including those responsible for polio, measles, rotaviral diarrhoea  and influenza infections, it is also an extremely powerful technique with important potential application for the prevention and control of a range of human and animal viral diseases.\u003c\/p\u003e \u003cp\u003e\u003ci\u003eReverse Genetics of RNA Viruses\u003c\/i\u003e provides a comprehensive account of the very latest developments in reverse genetics of RNA viruses through a wide range of applications within each of the core virus groups including; positive sense, negative sense and double stranded RNA viruses. Written by a team of international experts in the field, it provides a unique insight into how the field has developed, what problems are being addressed now and where applications may lead in the future. It will prove invaluable to bioscience, medical and veterinary students, those starting research in this area as well as other researchers and teachers needing to update their knowledge of this fast-moving field.\u003c\/p\u003e \u003cul\u003e \u003cli\u003eAn authoritative, comprehensive overview of reverse genetics in RNA Viruses.\u003c\/li\u003e \u003cli\u003eIncludes numerous examples of cutting- edge applications of reverse genetics within each of the RNA viral groups. \u003c\/li\u003e \u003cli\u003eWritten by a team of international experts, including some of the leading researchers in the field.\u003c\/li\u003e \u003c\/ul\u003e  List of contributors xi  \u003cp\u003eAcknowledgements xiii\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Introduction 1\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eAnne Bridgen\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e1.1 Background 1\u003c\/p\u003e \u003cp\u003e1.2 Reverse genetics for different classes of genome 2\u003c\/p\u003e \u003cp\u003e1.3 Methodology 5\u003c\/p\u003e \u003cp\u003e1.4 Difficulties in establishing a reverse genetics system 11\u003c\/p\u003e \u003cp\u003e1.5 Recent developments 13\u003c\/p\u003e \u003cp\u003e1.6 Are there any boundaries for conducting reverse genetics? 13\u003c\/p\u003e \u003cp\u003eReferences 15\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart I Positive sense RNA viruses 25\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Coronavirus reverse genetics 27\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eMaria Armesto, Kirsten Bentley, Erica Bickerton, Sarah Keep and Paul Britton\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2.1 The Coronavirinae 27\u003c\/p\u003e \u003cp\u003e2.2 Infectious bronchitis 28\u003c\/p\u003e \u003cp\u003e2.3 Coronavirus genome organisation 29\u003c\/p\u003e \u003cp\u003e2.4 The coronavirus replication cycle 30\u003c\/p\u003e \u003cp\u003e2.5 Development of reverse genetics system for coronaviruses including IBV 33\u003c\/p\u003e \u003cp\u003e2.6 Reverse genetics system for IBV 37\u003c\/p\u003e \u003cp\u003e2.7 Reverse genetics systems for the modification of coronavirus genomes 40\u003c\/p\u003e \u003cp\u003e2.8 Using coronavirus reverse genetics systems for gene delivery 49\u003c\/p\u003e \u003cp\u003eAcknowledgements 51\u003c\/p\u003e \u003cp\u003eReferences 51\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Reverse genetic tools to study hepatitis C virus 64\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eAlexander Ploss\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introduction: hepatitis C 64\u003c\/p\u003e \u003cp\u003e3.2 Hepatitis C virus 65\u003c\/p\u003e \u003cp\u003e3.3 Construction of infectious clones for hepatitis C virus 68\u003c\/p\u003e \u003cp\u003e3.4 Study of HCV RNA replication in cell culture systems 68\u003c\/p\u003e \u003cp\u003e3.5 Use of HCV replicons to study viral replication 70\u003c\/p\u003e \u003cp\u003e3.6 Utility of replicons for drug screening 71\u003c\/p\u003e \u003cp\u003e3.7 Development of the infectious cell culture systems for HCV 71\u003c\/p\u003e \u003cp\u003e3.8 Construction of intergenotypic viral chimeras 72\u003c\/p\u003e \u003cp\u003e3.9 Non-JFH1 derived genomes 74\u003c\/p\u003e \u003cp\u003e3.10 Cell lines that support HCV replication 74\u003c\/p\u003e \u003cp\u003e3.11 Study of HCV in physiologically more relevant cell culture systems 75\u003c\/p\u003e \u003cp\u003e3.12 Animal models for HCV infection 76\u003c\/p\u003e \u003cp\u003e3.13 Reverse genetics of clinically relevant HCV genotypes in vivo 77\u003c\/p\u003e \u003cp\u003e3.14 Conclusion 78\u003c\/p\u003e \u003cp\u003eAcknowledgments 78\u003c\/p\u003e \u003cp\u003eReferences 78\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Calicivirus reverse genetics 91\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eIan Goodfellow\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e4.1 Introduction 91\u003c\/p\u003e \u003cp\u003e4.2 Feline calicivirus 93\u003c\/p\u003e \u003cp\u003e4.3 Murine norovirus 97\u003c\/p\u003e \u003cp\u003e4.4 Porcine enteric calicivirus 103\u003c\/p\u003e \u003cp\u003e4.5 Rabbit haemorrhagic disease virus 104\u003c\/p\u003e \u003cp\u003e4.6 Human norovirus 104\u003c\/p\u003e \u003cp\u003e4.7 Conclusion 106\u003c\/p\u003e \u003cp\u003eAcknowledgements 107\u003c\/p\u003e \u003cp\u003eReferences 107\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart II Negative sense RNA viruses 113\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Reverse genetics of rhabdoviruses 115\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eAlexander Ghanem and Karl-Klaus Conzelmann\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction: the Rhabdoviridae family 115\u003c\/p\u003e \u003cp\u003e5.2 Rhabdovirus reverse genetics 121\u003c\/p\u003e \u003cp\u003e5.3 Applications and examples 132\u003c\/p\u003e \u003cp\u003e5.4 Conclusion 137\u003c\/p\u003e \u003cp\u003eAcknowledgements 137\u003c\/p\u003e \u003cp\u003eReferences 137\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Modification of measles virus and application to pathogenesis studies 150\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eLinda J. Rennick and W. Paul Duprex\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction 150\u003c\/p\u003e \u003cp\u003e6.2 Measles: the disease 150\u003c\/p\u003e \u003cp\u003e6.3 Measles: the infectious agent 151\u003c\/p\u003e \u003cp\u003e6.4 RNA synthesis: a tail of two processes 154\u003c\/p\u003e \u003cp\u003e6.5 Transcription: starting, stopping, dropping off or starting again 154\u003c\/p\u003e \u003cp\u003e6.6 From transcription to replication: the elusive switch 155\u003c\/p\u003e \u003cp\u003e6.7 Getting in and getting out 157\u003c\/p\u003e \u003cp\u003e6.8 Measles virus: reverse genetics 158\u003c\/p\u003e \u003cp\u003e6.9 Future perspectives 181\u003c\/p\u003e \u003cp\u003eAcknowledgements 182\u003c\/p\u003e \u003cp\u003eReferences 182\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Bunyavirus reverse genetics and applications to studying interactions with host cells 200\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eRichard M. Elliott\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction: the family Bunyaviridae 200\u003c\/p\u003e \u003cp\u003e7.2 Bunyavirus replication 201\u003c\/p\u003e \u003cp\u003e7.3 History of bunyavirus reverse genetics 203\u003c\/p\u003e \u003cp\u003e7.4 Minigenome systems for bunyaviruses 205\u003c\/p\u003e \u003cp\u003e7.5 Virus-like particle production 207\u003c\/p\u003e \u003cp\u003e7.6 Rescue systems for bunyaviruses 208\u003c\/p\u003e \u003cp\u003e7.7 Application of reverse genetics to study bunyavirus replication 208\u003c\/p\u003e \u003cp\u003e7.8 Outlook 215\u003c\/p\u003e \u003cp\u003eReferences 216\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Using reverse genetics to improve influenza vaccines 224\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eRuth A. Elderfield, Lorian C.S. Hartgroves and Wendy S. Barclay\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction 224\u003c\/p\u003e \u003cp\u003e8.2 Influenza vaccines 227\u003c\/p\u003e \u003cp\u003e8.3 The use of reverse genetics to generate recombinant influenza A, B and C viruses 229\u003c\/p\u003e \u003cp\u003e8.4 Using reverse genetics technology for generation of pandemic virus vaccine 232\u003c\/p\u003e \u003cp\u003e8.5 Other strategies for generating live attenuated vaccines based on viruses engineered by reverse genetics 235\u003c\/p\u003e \u003cp\u003e8.6 Strategies to improve the safety or yield of influenza vaccines 238\u003c\/p\u003e \u003cp\u003e8.7 Improvements to the PR8 high growth strain 239\u003c\/p\u003e \u003cp\u003e8.8 Improving the immunogenicity by engineering recombinant viruses that express cytokine genes 240\u003c\/p\u003e \u003cp\u003e8.9 Novel species-specific attenuation that takes advantage of microRNAs 240\u003c\/p\u003e \u003cp\u003e8.10 Conclusion 241\u003c\/p\u003e \u003cp\u003eReferences 241\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart III Double-stranded RNA viruses 251\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Bluetongue virus reverse genetics 253\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eMark Boyce\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction to Bluetongue virus 253\u003c\/p\u003e \u003cp\u003e9.2 Bluetongue virus replication 254\u003c\/p\u003e \u003cp\u003e9.3 Reverse genetics 260\u003c\/p\u003e \u003cp\u003e9.4 Uses of reverse genetics in orbivirus research 271\u003c\/p\u003e \u003cp\u003e9.5 Future perspectives 278\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Genetic modification in mammalian orthoreoviruses 289\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eSanne K. van den Hengel, Iris J.C. Dautzenberg, Diana J.M. van den Wollenberg, Peter A.E. Sillevis Smitt and Rob C. Hoeben\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e10.1 Introduction 289\u003c\/p\u003e \u003cp\u003e10.2 Forward-genetics in orthoreoviruses 296\u003c\/p\u003e \u003cp\u003e10.3 Reovirus\/cell interactions 297\u003c\/p\u003e \u003cp\u003e10.4 Reverse-genetics in orthoreoviruses 301\u003c\/p\u003e \u003cp\u003e10.5 Reovirus as an oncolytic agent 306\u003c\/p\u003e \u003cp\u003e10.6 Conclusion 308\u003c\/p\u003e \u003cp\u003eReferences 309\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart IV Recent and future developments 319\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Reverse genetics and quasispecies 321\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eAntonio V. Border´ýa and Marco Vignuzzi\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e11.1 Definition of quasispecies and evidence 321\u003c\/p\u003e \u003cp\u003e11.2 Reverse genetics and RNA virus population heterogeneity: consensus is always a compromise 328\u003c\/p\u003e \u003cp\u003e11.3 Examples of the use of the theory to disable or manipulate the quasispecies under controlled environments 333\u003c\/p\u003e \u003cp\u003e11.4 Future prospects of virus population genetics and reverse genetics 339\u003c\/p\u003e \u003cp\u003e11.5 Conclusion 341\u003c\/p\u003e \u003cp\u003eReferences 342\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Summary and perspectives 350\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eAnne Bridgen\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction 350\u003c\/p\u003e \u003cp\u003e12.2 Analysis of the role of specific non-coding sequence motifs involved in replication, transcription, polyadenylation and packaging 351\u003c\/p\u003e \u003cp\u003e12.3 Analysis of the roles of viral proteins 352\u003c\/p\u003e \u003cp\u003e12.4 Analysis of virus–host interactions at a global level 353\u003c\/p\u003e \u003cp\u003e12.5 Understanding the basis of pathogenicity 354\u003c\/p\u003e \u003cp\u003e12.6 Real-time virus imaging in vitro and in vivo 355\u003c\/p\u003e \u003cp\u003e12.7 Structure-function analysis of viruses and viral domains 356\u003c\/p\u003e \u003cp\u003e12.8 Vaccine generation 357\u003c\/p\u003e \u003cp\u003e12.9 Drug development 359\u003c\/p\u003e \u003cp\u003e12.10 Gene delivery and knock-out in plant cells including virus-induced gene silencing (VIGS) 361\u003c\/p\u003e \u003cp\u003e12.11 Gene delivery in arthropod and mammalian cells 362\u003c\/p\u003e \u003cp\u003e12.12 Development of oncolytic virus and adaptation to this purpose 363\u003c\/p\u003e \u003cp\u003e12.13 Personal highlights and future directions 364\u003c\/p\u003e \u003cp\u003eReferences 366\u003c\/p\u003e \u003cp\u003eIndex 375\u003c\/p\u003e \u003cp\u003e\u003cb\u003eDr Anne Bridgen\u003c\/b\u003e, previously of The University of Ulster is a molecular virologist with extensive research and teaching experience. She was the first scientist to recover infectious virus particles from DNA clones of a segmented RNA virus. Dr Bridgen knows the field and its main players well and has both the knowledge and experience to bring individual expert contributions together around the common theme of reverse genetics.\u003cbr\u003eShe is currently providing consultancy for a BBSRC grant based at IAH Pirbright.\u003c\/p\u003e  \u003cp\u003eReverse genetics, the genetic manipulation of RNA viruses to create a wild-type or modified virus, has led to important advances in our understanding of viral gene function and interaction with host cells. Since many severe viral human and animal pathogens are RNA viruses, including those responsible for polio, measles, rotaviral diarrhoea  and influenza infections, it is also an extremely powerful technique with important potential application for the prevention and control of a range of human and animal viral diseases.\u003c\/p\u003e \u003cp\u003e\u003ci\u003eReverse Genetics of RNA Viruses\u003c\/i\u003e provides a comprehensive account of the very latest developments in reverse genetics of RNA viruses through a wide range of applications within each of the core virus groups including; positive sense, negative sense and double stranded RNA viruses. Written by a team of international experts in the field, it provides a unique insight into how the field has developed, what problems are being addressed now and where applications may lead in the future. It will prove invaluable to bioscience, medical and veterinary students, those starting research in this area as well as other researchers and teachers needing to update their knowledge of this fast-moving field.\u003c\/p\u003e \u003cul\u003e \u003cli\u003eAn authoritative, comprehensive overview of reverse genetics in RNA Viruses.\u003c\/li\u003e \u003cli\u003eIncludes numerous examples of cutting- edge applications of reverse genetics within each of the RNA viral groups. \u003c\/li\u003e \u003cli\u003eWritten by a team of international experts, including some of the leading researchers in the field.\u003c\/li\u003e \u003c\/ul\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47989959000293,"sku":"NP9780470979655","price":184.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9780470979655.jpg?v=1761786032","url":"https:\/\/k12savings.com\/products\/reverse-genetics-of-rna-viruses-isbn-9780470979655","provider":"K12savings","version":"1.0","type":"link"}