{"product_id":"replacing-animal-models-isbn-9780470974254","title":"Replacing Animal Models","description":"Over the last decade, in vitro models have become more sophisticated and are at a stage where they can provide an effective alternative to in vivo experiments. \u003ci\u003eReplacing Animal Models\u003c\/i\u003e provides scientists and technicians with a practical, integrated guide to developing culture-based alternatives to in vivo experiments.  \u003cp\u003eThe book is neither political nor polemical: it is technical, illustrating by example how alternatives can be developed and used and providing useful advice on developing others. After looking at the reasons for and potential benefits of alternatives to animal experiments, the book covers a range of methods and examples emphasising the design considerations that went into each system. The chapters also include 'case studies' that illustrate the ways in which culture models can be used to answer a range of important biological questions of direct relevance to human development, physiology, disease and healing.\u003c\/p\u003e \u003cp\u003eThe thesis of this book is not that all animal experimentation can be replaced, now or in the near future, by equally effective or superior alternatives. Rather, the premise is that there is substantial opportunity, here and now, to do some common types of experiment better in vitro than in vivo, and that doing so will result in both scientific and ethical gains.\u003c\/p\u003e  Contributors, vii  \u003cp\u003ePreface, xi\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSection 1 Introductory Material\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1 Potential Advantages of Using Biomimetic Alternatives, 3\u003cbr\u003e \u003ci\u003eJamie Davies\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2 Overview of Biomimetic Alternatives, 13\u003cbr\u003e \u003ci\u003eJamie Davies\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSection 2 Culture Methods\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3 Pancreatic Islets, 23\u003cbr\u003e \u003ci\u003eEli C Lewis\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e4 Endometrial Organoid Culture, 35\u003cbr\u003e \u003ci\u003eMerja Blauer\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5 Modelling Lymphatic and Blood Capillary Patterning, 45\u003cbr\u003e \u003ci\u003eFrancoise Bruyere, Catherine Maillard, Charlotte Erpicum and Agnes Noel\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6 Precision-cut Lung Slices (PCLS), 57\u003cbr\u003e \u003ci\u003eChristian Martin and Stefan Uhlig\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7 Human Colon Tissue in Organ Culture, 69\u003cbr\u003e \u003ci\u003eMichael K Dame and James Varani\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e8 Fetal Organ Culture, 81\u003cbr\u003e \u003ci\u003eJamie Davies\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e9 Design of a Mechanical Loading Device to Culture Intact Bovine Spinal Motion Segments under Multiaxial Motion, 89\u003cbr\u003e \u003ci\u003eJochen Walser, Stephen John Ferguson and Benjamin Gantenbein-Ritter\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e10 Magnetic Assembly of Tissue Surrogates, 107\u003cbr\u003e \u003ci\u003eChien-Yu Fu and Hwan-You Chang\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e11 Assembly of Renal Tissues by Cellular Self-organization, 115\u003cbr\u003e \u003ci\u003eMathieu Unbekandt\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSection 3 Case Studies of Use\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e12 Hierarchical Screening of Pathways: Using Cell and Organ Cultures to Reduce use of Transgenic Mice, 125\u003cbr\u003e \u003ci\u003eGuangping Tai and Jamie Davies\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e13 Lung Organoid Culture to Study Responses to Viruses, 137\u003cbr\u003e \u003ci\u003eWenxin Wu, J Leland Booth and Jordan P Metcalf\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e14 Organ-cultured Human Skin for the Study of Epithelial Cell Invasion of Stroma, 151\u003cbr\u003e \u003ci\u003eJames Varani\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e15 Organotypic Mandibular Cultures for the Study of Inflammatory Bone Pathology, 159\u003cbr\u003e \u003ci\u003eAlastair J Sloan, Sarah Y Taylor and Emma L Smith\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e16 Three-dimensional, High-density and Tissue Engineered Culture Models of Articular Cartilage, 167\u003cbr\u003e \u003ci\u003eAli Mobasheri, Sara Kelly, Abigail L. Clutterbuck, Constanze Buhrmann and Mehdi Shakibaei\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e17 Concluding Remarks, 193\u003cbr\u003e \u003ci\u003eJamie Davies\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendix 1\u003c\/b\u003e Sources of funding for development of culture-based alternatives, 195\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendix 2\u003c\/b\u003e Databases and web-based discussions relevant to development of alternatives, 197\u003c\/p\u003e \u003cp\u003eIndex, 199\u003c\/p\u003e \u003cb\u003eProfessor Jamie A. Davies\u003c\/b\u003e, Centre for Integrative Physiology, Hugh Robson Building, George Square, University of Edinburgh, Edinburgh.  \u003cp\u003eOver the last decade, in vitro models have become more sophisticated and are at a stage where they can provide an effective alternative to in \u003ci\u003evivo\u003c\/i\u003e experiments. \u003ci\u003eReplacing Animal Models\u003c\/i\u003e provides scientists and technicians with a practical, integrated guide to developing culture-based alternatives to in \u003ci\u003evivo\u003c\/i\u003e experiments.\u003cbr\u003e \u003cbr\u003e The book is neither political nor polemical: it is technical, illustrating by example how alternatives can be developed and used and providing useful advice on developing others. After looking at the reasons for the potential benefits of alternatives to animal experiments, the book covers a range of methods and examples emphasising the design considerations that went into each system. The chapters also include ‘case studies’ that illustrate the ways in which culture models can be used to answer a range of important biological questions of direct relevance to human development, physiology, disease and healing.\u003cbr\u003e \u003cbr\u003e The thesis of this book is not that all animal experimentation can be replaced, now or in the near future, by equally effective or superior alternatives. Rather, the premise is that there is substantial opportunity, here and now, to do some common types of experiment better in \u003ci\u003evitro\u003c\/i\u003e than in \u003ci\u003evivo\u003c\/i\u003e, and that doing so well result in both scientific and ethical gains.\u003c\/p\u003e","brand":"Wiley-Blackwell","offers":[{"title":"Default Title","offer_id":47989944680677,"sku":"NP9780470974254","price":113.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9780470974254.jpg?v=1761785988","url":"https:\/\/k12savings.com\/products\/replacing-animal-models-isbn-9780470974254","provider":"K12savings","version":"1.0","type":"link"}