{"product_id":"quantitative-applications-of-mass-spectrometry-isbn-9780470025161","title":"Quantitative Applications of Mass Spectrometry","description":"This text presents the information needed to design a successful quantitative analysis using mass spectrometric techniques currently available and widely employed. It is devoted to the researchers of different areas, who use mass spectrometry as a detector suitable for the measurements of their interest.  \u003cul\u003e \u003cli\u003eAn essential book for the practicing mass spectroscopist\u003c\/li\u003e \u003cli\u003eA genuine 'how-to' text for the practitioner focusing on quantification rather than instrumental design and techniques\u003c\/li\u003e \u003cli\u003eUp-to-date structured text describing methods, experimental strategy, capabilities and limitations, with data analysis and interpretation\u003c\/li\u003e \u003cli\u003eBrings together material widely dispersed in the pertinent literature into one unique source\u003c\/li\u003e \u003cli\u003eInternationally recognized group of authors\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003ePreface ix\u003c\/p\u003e \u003cp\u003eAcknowledgements xi\u003c\/p\u003e \u003cp\u003eIntroduction xiii\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 What Instrumental Approaches are Available 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 Ion Sources 1\u003c\/p\u003e \u003cp\u003e1.1.1 Electron Ionization 3\u003c\/p\u003e \u003cp\u003e1.1.2 Chemical Ionization 4\u003c\/p\u003e \u003cp\u003e1.1.3 Atmospheric Pressure Chemical Ionization 6\u003c\/p\u003e \u003cp\u003e1.1.4 Electrospray Ionization 8\u003c\/p\u003e \u003cp\u003e1.1.5 Atmospheric Pressure Photoionization 11\u003c\/p\u003e \u003cp\u003e1.1.6 Matrix-assisted Laser Desorption\/Ionization 12\u003c\/p\u003e \u003cp\u003e1.2 Mass Analysers 14\u003c\/p\u003e \u003cp\u003e1.2.1 Mass Resolution 14\u003c\/p\u003e \u003cp\u003e1.2.2 Sector Analysers 15\u003c\/p\u003e \u003cp\u003e1.2.3 Quadrupole Analysers 19\u003c\/p\u003e \u003cp\u003e1.2.4 Time-of-flight 25\u003c\/p\u003e \u003cp\u003e1.3 GC\/MS 27\u003c\/p\u003e \u003cp\u003e1.3.1 Total Ion Current (TIC) Chromatogram 27\u003c\/p\u003e \u003cp\u003e1.3.2 Reconstructed Ion Chromatogram (RIC) 28\u003c\/p\u003e \u003cp\u003e1.3.3 Multiple Ion Detection (MID) 29\u003c\/p\u003e \u003cp\u003e1.4 LC\/MS 29\u003c\/p\u003e \u003cp\u003e1.5 MS\/MS 30\u003c\/p\u003e \u003cp\u003e1.5.1 MS\/MS by Double Focusing Instruments 30\u003c\/p\u003e \u003cp\u003e1.5.2 MS\/MS by Triple Quadrupoles 31\u003c\/p\u003e \u003cp\u003e1.5.3 MS\/MS by Ion Traps 32\u003c\/p\u003e \u003cp\u003e1.5.4 MS\/MS by Q-TOF 34\u003c\/p\u003e \u003cp\u003eReferences 34\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 How to Design a Quantitative Analysis 37\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 General Strategy 38\u003c\/p\u003e \u003cp\u003e2.1.1 Project 41\u003c\/p\u003e \u003cp\u003e2.1.2 Sampling 41\u003c\/p\u003e \u003cp\u003e2.1.3 Sample Treatment 42\u003c\/p\u003e \u003cp\u003e2.1.4 Instrumental Analysis 43\u003c\/p\u003e \u003cp\u003e2.1.5 Method Validation 53\u003c\/p\u003e \u003cp\u003eReferences 53\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 How to Improve Specificity 55\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Choice of a Suitable Chromatographic Procedure 56\u003c\/p\u003e \u003cp\u003e3.1.1 GC\/MS Measurements in Low and High Resolution Conditions 56\u003c\/p\u003e \u003cp\u003e3.1.2 LC\/ESI\/MS and LC\/APCI\/MS Measurements 61\u003c\/p\u003e \u003cp\u003e3.2 Choice of a Suitable Ionization Method 79\u003c\/p\u003e \u003cp\u003e3.3 An Example of High Specificity and Selectivity Methods: The Dioxin Analysis 85\u003c\/p\u003e \u003cp\u003e3.3.1 Use of High Resolution MID Analysis 85\u003c\/p\u003e \u003cp\u003e3.3.2 NICI in the Analysis of Dioxins, Furans and PCBs 93\u003c\/p\u003e \u003cp\u003e3.3.3 MS\/MS in the Detection of Dioxins, Furans and PCBs 95\u003c\/p\u003e \u003cp\u003e3.4 An Example of MALDI\/MS in Quantitative Analysis of Polypeptides: Substance P 101\u003c\/p\u003e \u003cp\u003eReferences 106\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Some Thoughts on Calibration and Data Analysis 107\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Calibration Designs 108\u003c\/p\u003e \u003cp\u003e4.2 Homoscedastic and Heteroscedastic Data 108\u003c\/p\u003e \u003cp\u003e4.2.1 Variance Model 109\u003c\/p\u003e \u003cp\u003e4.3 Calibration Models 109\u003c\/p\u003e \u003cp\u003e4.3.1 Unweighted Regression 109\u003c\/p\u003e \u003cp\u003e4.3.2 Weighted Regression 119\u003c\/p\u003e \u003cp\u003e4.3.3 A Practical Example 126\u003c\/p\u003e \u003cp\u003e4.4 Different Approaches to Estimate Detection and Quantification Limits 130\u003c\/p\u003e \u003cp\u003eReferences 132\u003c\/p\u003e \u003cp\u003eIndex 135\u003c\/p\u003e \"…anyone needing to do serious quantitative work using GC\/MS could learn from these authors.\" (\u003ci\u003eCHOICE\u003c\/i\u003e, January 2007)  \u003cb\u003eProfessor Pietro Traldi\u003c\/b\u003e, Research Executive, National Council of Research, CNR, ISTM Corso Stati Uniti 4 I35127 Padova, Italy  \u003cp\u003eFields of interest include structure and reactivity of gaseous ions; -MS application in environmental and biomedical fields; Development of new analytical methods; Instrumental developments. Pietro Traldi is author of over 500 publications on international journals; he is member of the advisory boards of JMS, MSR, RCM and EMS. He is the promoter and the chairman of the Informal Meeting of Mass Spectrometry.\u003c\/p\u003e \u003cp\u003e\u003cb\u003eProfessor Irma Lavagnini,\u003c\/b\u003e Associate Professor in Analytical Chemistry, Faculty of Pharmacy. Scientific research focus is Chemical data handling\u003c\/p\u003e \u003cp\u003e\u003cb\u003eProfessor Franco Magno,\u003c\/b\u003e Padua University, Italy, Full Professor of Analytical Chemistry since 1980. Main research areas in electroanalytical techniques; Development and application of the digital simulation techniques to rationalize voltammetric responses; Development and optimization of analytical procedures to determine species of relevant enviromental and\/or industrial interest.\u003ci\u003e\u003cbr\u003e \u003cbr\u003e \u003c\/i\u003e\u003cb\u003eDr Roberta Seraglia,\u003c\/b\u003e National Council of Research, Padova, Italy, Researcher of CNR, Institute of Molecular Sciences and Technologies, Padova. 130 papers published in International Journals and 160 communications at International and National congresses.\u003c\/p\u003e \u003cp\u003eWhat is usually required of an analytical technique? Specificity, selectivity and sensitivity -or, more precisely, a detection limit as low as possible. These criteria are fulfilled by mass spectrometry is an essential tool for qualitative and quantitative investigations in many applications, in particular in environmental science and biomedicine,with positive benefits to mankind.\u003c\/p\u003e \u003cp\u003eThe problem that has arisen from this large expansion is that mass spectrometry is mainly considered as a \"magic box\" technique, in which on one side a sample is introduced and on the other side the analytical data comes out. Thus, the basic aspects of the technique are often not well known. To overcome this, the authors present within this text some basic information for scientists and technicians working in the field of quantitative organic mass spectrometry, in particular for those who, for the first time, face all the problems arising from the development and use of a quantitative procedure.\u003c\/p\u003e \u003cp\u003eThis informative text presents basic information on:\u003c\/p\u003e \u003cul\u003e \u003cli\u003eWhat instrumental approaches are available\u003c\/li\u003e \u003cli\u003eHow to design a quantitative analysis\u003c\/li\u003e \u003cli\u003eHow to improve specificity\u003c\/li\u003e \u003cli\u003eSome thoughts on calibration and data analysis\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThis book is mainly aimed at analytical chemists working in academic, environmental, pharmaceutical, biochemical, forensic, clinical and industrial laboratories, but is also appropriate for researchers new to the technique, e.g. biochemists, biologist, physicians and workers in pharmaceutical, food and the health sciences.\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47989895659749,"sku":"NP9780470025161","price":59.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9780470025161.jpg?v=1761785828","url":"https:\/\/k12savings.com\/products\/quantitative-applications-of-mass-spectrometry-isbn-9780470025161","provider":"K12savings","version":"1.0","type":"link"}