{"product_id":"protein-purification-isbn-9780471746614","title":"Protein Purification","description":"\u003cp\u003e\u003cb\u003eThe authoritative guide on protein purification—now completely updated and revised\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eSince the Second Edition of \u003ci\u003eProtein Purification\u003c\/i\u003e was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch.\u003c\/p\u003e \u003cp\u003eLeading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes:\u003c\/p\u003e \u003cul\u003e \u003cli\u003e \u003cp\u003eHigh-resolution reversed-phase liquid chromatography\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eElectrophoresis in gels\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eConventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eAffinity ligands from chemical and biological combinatorial libraries\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eMembrane separations\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eRefolding of inclusion body proteins from E. coli\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003ePurification of PEGylated proteins\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eHigh throughput screening techniques in protein purification\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eThe history of protein chromatography\u003c\/p\u003e \u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003ePreface to The Third Edition vii\u003c\/p\u003e \u003cp\u003ePreface to The Second Edition ix\u003c\/p\u003e \u003cp\u003ePreface to The First Edition xi\u003c\/p\u003e \u003cp\u003eContributors xiii\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart I Introduction 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1 Introduction to Protein Purification 3\u003cbr\u003e \u003ci\u003eBo Ersson, Lars Ryd\u003c\/i\u003e\u003ci\u003eén, and Jan-Christer Janson\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart II Chromatography 23\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2 Introduction to Chromatography 25\u003cbr\u003e \u003ci\u003eJan-Christer Janson and Jan \u003c\/i\u003e\u003ci\u003eÅke J\u003c\/i\u003e\u003ci\u003eönsson\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3 Gel Filtration: Size Exclusion Chromatography 51\u003cbr\u003e \u003ci\u003eLars Hagel\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e4 Ion Exchange Chromatography 93\u003cbr\u003e \u003ci\u003eEvert Karlsson and Irwin Hirsh\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5 High-Resolution Reversed-Phase Chromatography of Proteins 135\u003cbr\u003e \u003ci\u003eSylvia Winkel Pettersson\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6 Hydrophobic Interaction Chromatography 165\u003cbr\u003e \u003ci\u003eKjell-Ove Eriksson and Makonnen Belew\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7 Immobilized Metal Ion Affinity Chromatography 183\u003cbr\u003e \u003ci\u003eLennart Kågedal\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e8 Covalent Chromatography 203\u003cbr\u003e \u003ci\u003eFrancisco Batista-Viera, Lars Ryd\u003c\/i\u003e\u003ci\u003eén, and Jan Carlsson\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e9 Affinity Chromatography 221\u003cbr\u003e \u003ci\u003eFrancisco Batista-Viera, Jan-Christer Janson, and Jan Carlsson\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e10 Affinity Ligands from Chemical Combinatorial Libraries 259\u003cbr\u003e \u003ci\u003eEnrique Carredano and Herbert Baumann\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e11 Affinity Ligands from Biological Combinatorial Libraries 269\u003cbr\u003e \u003ci\u003ePer-\u003c\/i\u003e\u003ci\u003e Åke Nygren\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart III Other Separation Methods and Related Techniques 279\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e12 Membrane Separations 281\u003cbr\u003e \u003ci\u003eJoachim K. Walter, Zuwei Jin, Maik W. Jornitz, and Uwe Gottschalk\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e13 Refolding of Inclusion Body Proteins from E. coli 319\u003cbr\u003e \u003ci\u003eZhiguo Su, Diannan Lu, and Zheng Liu\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e14 Purification of PEGylated Proteins 339\u003cbr\u003e \u003ci\u003eConan J. Fee and James M. Van Alstine\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart IV Electrophoresis 363\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e15 Electrophoresis in Gels 365\u003cbr\u003e \u003ci\u003eReiner Westermeier\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e16 Conventional Isoelectric Focusing in Gel Slabs and Capillaries and Immobilized pH Gradients 379\u003cbr\u003e \u003ci\u003ePier Giorgio Righetti, Elisa Fasoli, and Sabina Carla Righetti\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e17 Two-Dimensional Electrophoresis in Proteomics 411\u003cbr\u003e \u003ci\u003eReiner Westermeier and Angelika G\u003c\/i\u003e\u003ci\u003eörg\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e18 Protein Elution and Blotting Techniques 441\u003cbr\u003e \u003ci\u003eReiner Westermeier\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e19 Capillary Electrophoretic Separations 451\u003cbr\u003e \u003ci\u003eWolfgang Thormann\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart V Separation Method Optimization 487\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e20 High Throughput Screening Techniques in Protein Purification 489\u003cbr\u003e \u003ci\u003eKarol M. Lacki and Eggert Brekkan\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eIndex 507\u003c\/p\u003e \u003cp\u003eJAN-CHRISTER JANSON is Professor Emeritus at the Department of Physical and Analytical Chemistry, Uppsala University, Uppsala, Sweden.\u003c\/p\u003e   \u003cp\u003eThe authoritative guide on protein purification—now completely updated and revised\u003c\/p\u003e \u003cp\u003eSince the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch.\u003c\/p\u003e \u003cp\u003eLeading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes:\u003c\/p\u003e \u003cul\u003e \u003cli\u003e \u003cp\u003eHigh-resolution reversed-phase liquid chromatography\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eElectrophoresis in gels\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eConventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eAffinity ligands from chemical and biological combinatorial libraries\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eMembrane separations\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eRefolding of inclusion body proteins from E. coli\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003ePurification of PEGylated proteins\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eHigh throughput screening techniques in protein purification\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eThe history of protein chromatography\u003c\/p\u003e \u003c\/li\u003e \u003c\/ul\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47989880455397,"sku":"NP9780471746614","price":171.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9780471746614.jpg?v=1761785773","url":"https:\/\/k12savings.com\/products\/protein-purification-isbn-9780471746614","provider":"K12savings","version":"1.0","type":"link"}