{"product_id":"principles-of-virology-volume-1-isbn-9781683672845","title":"Principles of Virology, Volume 1","description":"\u003ci\u003ePrinciples of Virology\u003c\/i\u003e, the leading virology textbook in use, is an extremely valuable and highly informative presentation of virology at the interface of modern cell biology and immunology. This text utilizes a uniquely rational approach by highlighting common principles and processes across all viruses. Using a set of representative viruses to illustrate the breadth of viral complexity, students are able to under-stand viral reproduction and pathogenesis and are equipped with the necessary tools for future encounters with new or understudied viruses.\u003cbr\u003e\u003cbr\u003eThis fifth edition was updated to keep pace with the ever-changing field of virology. In addition to the beloved full-color illustrations, video interviews with leading scientists, movies, and links to exciting blogposts on relevant topics, this edition includes study questions and active learning puzzles in each chapter, as well as short descriptions regarding the key messages of references of special interest. \u003cbr\u003e\u003cbr\u003e\u003ci\u003eVolume I: Molecular Biology\u003c\/i\u003e focuses on the molecular processes of viral reproduction, from entry through release. \u003ci\u003eVolume II: Pathogenesis and Control\u003c\/i\u003e addresses the interplay between viruses and their host organisms, on both the micro- and macroscale, including chapters on public health, the immune response, vaccines and other antiviral strategies, viral evolution, and a brand new chapter on the therapeutic uses of viruses. These two volumes can be used for separate courses or together in a single course. Each includes a unique appendix, glossary, and links to internet resources.\u003cbr\u003e\u003cbr\u003e\u003ci\u003ePrinciples of Virology, Fifth Edition\u003c\/i\u003e, is ideal for teaching the strategies by which all viruses reproduce, spread within a host, and are maintained within populations. This edition carefully reflects the results of extensive vetting and feedback received from course instructors and students, making this renowned textbook even more appropriate for undergraduate and graduate courses in virology, microbiology, and infectious diseases. \u003cp\u003ePreface xvii\u003c\/p\u003e \u003cp\u003eAcknowledgments xxi\u003c\/p\u003e \u003cp\u003eAbout the Authors xxiii\u003c\/p\u003e \u003cp\u003eKey of Repetitive Elements xxv\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart I The Science of Virology 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Foundations 2\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eLuria’s Credo 3\u003c\/p\u003e \u003cp\u003eViruses Defined 3\u003c\/p\u003e \u003cp\u003eWhy We Study Viruses 3\u003c\/p\u003e \u003cp\u003eViruses Are Everywhere 3\u003c\/p\u003e \u003cp\u003eViruses Infect All Living Things 4\u003c\/p\u003e \u003cp\u003eViruses Can Cause Human Disease 5\u003c\/p\u003e \u003cp\u003eViruses Can Be Beneficial 5\u003c\/p\u003e \u003cp\u003eViruses “R” Us 6\u003c\/p\u003e \u003cp\u003eViruses Can Cross Species Boundaries 6\u003c\/p\u003e \u003cp\u003eViruses Are Unique Tools To Study Biology 6\u003c\/p\u003e \u003cp\u003eVirus Prehistory 7\u003c\/p\u003e \u003cp\u003eViral Infections in Antiquity 7\u003c\/p\u003e \u003cp\u003eThe First Vaccines 8\u003c\/p\u003e \u003cp\u003eMicroorganisms as Pathogenic Agents 9\u003c\/p\u003e \u003cp\u003eDiscovery of Viruses 11\u003c\/p\u003e \u003cp\u003eThe Defining Properties of Viruses 13\u003c\/p\u003e \u003cp\u003eThe Structural Simplicity of Virus Particles 13\u003c\/p\u003e \u003cp\u003eThe Intracellular Parasitism of Viruses 13\u003c\/p\u003e \u003cp\u003eCataloging Animal Viruses 18\u003c\/p\u003e \u003cp\u003eThe Classical System 18\u003c\/p\u003e \u003cp\u003eClassification by Genome Type: the Baltimore System 19\u003c\/p\u003e \u003cp\u003eA Common Strategy for Viral Propagation 21\u003c\/p\u003e \u003cp\u003ePerspectives 21\u003c\/p\u003e \u003cp\u003eReferences 24\u003c\/p\u003e \u003cp\u003eStudy Questions 24\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 The Infectious Cycle 26\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 27\u003c\/p\u003e \u003cp\u003eThe Infectious Cycle 27\u003c\/p\u003e \u003cp\u003eThe Cell 27\u003c\/p\u003e \u003cp\u003eEntering Cells 28\u003c\/p\u003e \u003cp\u003eViral RNA Synthesis 29\u003c\/p\u003e \u003cp\u003eViral Protein Synthesis 29\u003c\/p\u003e \u003cp\u003eViral Genome Replication 29\u003c\/p\u003e \u003cp\u003eAssembly of Progeny Virus Particles 29\u003c\/p\u003e \u003cp\u003eViral Pathogenesis 29\u003c\/p\u003e \u003cp\u003eOvercoming Host Defenses 30\u003c\/p\u003e \u003cp\u003eCultivation of Viruses 30\u003c\/p\u003e \u003cp\u003eCell Culture 30\u003c\/p\u003e \u003cp\u003eEmbryonated Eggs 35\u003c\/p\u003e \u003cp\u003eLaboratory Animals 35\u003c\/p\u003e \u003cp\u003eAssay of Viruses 35\u003c\/p\u003e \u003cp\u003eMeasurement of Infectious Units 35\u003c\/p\u003e \u003cp\u003eEfficiency of Plating 38\u003c\/p\u003e \u003cp\u003eMeasurement of Virus Particles 40\u003c\/p\u003e \u003cp\u003eViral Reproduction: The Burst Concept 49\u003c\/p\u003e \u003cp\u003eThe One-Step Growth Cycle 49\u003c\/p\u003e \u003cp\u003eOne-Step Growth Analysis: a Valuable Tool for Studying Animal Viruses 52\u003c\/p\u003e \u003cp\u003eGlobal Analysis 53\u003c\/p\u003e \u003cp\u003eDNA Microarrays 54\u003c\/p\u003e \u003cp\u003eMass Spectrometry 56\u003c\/p\u003e \u003cp\u003eProtein-Protein Interactions 56\u003c\/p\u003e \u003cp\u003eSingle-Cell\u003c\/p\u003e \u003cp\u003eVirology 56\u003c\/p\u003e \u003cp\u003ePerspectives 58\u003c\/p\u003e \u003cp\u003eReferences 59\u003c\/p\u003e \u003cp\u003eStudy Questions 60\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart II Molecular Biology 61\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Genomes and Genetics 62\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 63\u003c\/p\u003e \u003cp\u003eGenome Principles and the Baltimore System 63\u003c\/p\u003e \u003cp\u003eStructure and Complexity of Viral Genomes 63\u003c\/p\u003e \u003cp\u003eDNA Genomes 64\u003c\/p\u003e \u003cp\u003eRNA Genomes 65\u003c\/p\u003e \u003cp\u003eWhat Do Viral Genomes Look Like? 68\u003c\/p\u003e \u003cp\u003eCoding Strategies 69\u003c\/p\u003e \u003cp\u003eWhat Can Viral Sequences Tell Us? 69\u003c\/p\u003e \u003cp\u003eThe “Big and Small” of Viral Genomes: Does Size Matter? 71\u003c\/p\u003e \u003cp\u003eThe Origin of Viral Genomes 73\u003c\/p\u003e \u003cp\u003eGenetic Analysis of Viruses 74\u003c\/p\u003e \u003cp\u003eClassical Genetic Methods 75\u003c\/p\u003e \u003cp\u003eEngineering Mutations into Viral Genomes 77\u003c\/p\u003e \u003cp\u003eEngineering Viral Genomes: Viral Vectors 83\u003c\/p\u003e \u003cp\u003ePerspectives 87\u003c\/p\u003e \u003cp\u003eReferences 87\u003c\/p\u003e \u003cp\u003eStudy Questions 88\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Structure 90\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 91\u003c\/p\u003e \u003cp\u003eFunctions of the Virion 91\u003c\/p\u003e \u003cp\u003eNomenclature 92\u003c\/p\u003e \u003cp\u003eMethods for Studying Virus Structure 92\u003c\/p\u003e \u003cp\u003eBuilding a Protective Coat 95\u003c\/p\u003e \u003cp\u003eHelical Structures 96\u003c\/p\u003e \u003cp\u003eCapsids with Icosahedral Symmetry 99\u003c\/p\u003e \u003cp\u003eOther Capsid Architectures 111\u003c\/p\u003e \u003cp\u003ePackaging the Nucleic Acid Genome 112\u003c\/p\u003e \u003cp\u003eDirect Contact of the Genome with a Protein Shell 112\u003c\/p\u003e \u003cp\u003ePackaging by Specialized Viral Proteins 113\u003c\/p\u003e \u003cp\u003ePackaging by Cellular Proteins 113\u003c\/p\u003e \u003cp\u003eViruses with Envelopes 115\u003c\/p\u003e \u003cp\u003eViral Envelope Components 115\u003c\/p\u003e \u003cp\u003eSimple Enveloped Viruses: Direct Contact of External Proteins with the Capsid or Nucleocapsid 117\u003c\/p\u003e \u003cp\u003eEnveloped Viruses with an Additional Protein Layer 118\u003c\/p\u003e \u003cp\u003eLarge Viruses with Multiple Structure Elements 119\u003c\/p\u003e \u003cp\u003eParticles with Helical or Icosahedral Parts 120\u003c\/p\u003e \u003cp\u003eAlternative Architectures 123\u003c\/p\u003e \u003cp\u003eOther Components of Virions 125\u003c\/p\u003e \u003cp\u003eEnzymes 125\u003c\/p\u003e \u003cp\u003eOther Viral Proteins 125\u003c\/p\u003e \u003cp\u003eCellular Macromolecules 126\u003c\/p\u003e \u003cp\u003eMechanical Properties of Virus Particles 126\u003c\/p\u003e \u003cp\u003eInvestigation of Mechanical Properties of Virus Particles 126\u003c\/p\u003e \u003cp\u003eStabilization and Destabilization of Virus Particles 128\u003c\/p\u003e \u003cp\u003ePerspectives 128\u003c\/p\u003e \u003cp\u003eReferences 129\u003c\/p\u003e \u003cp\u003eStudy Questions 130\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Attachment and Entry 132\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 133\u003c\/p\u003e \u003cp\u003eAttachment of Virus Particles to Cells 133\u003c\/p\u003e \u003cp\u003eGeneral Principles 133\u003c\/p\u003e \u003cp\u003eIdentification of Receptors for Virus Particles 135\u003c\/p\u003e \u003cp\u003eVirus-Receptor Interactions 137\u003c\/p\u003e \u003cp\u003eEntry into Cells 142\u003c\/p\u003e \u003cp\u003eVirus-induced Signaling via Cell Receptors 142\u003c\/p\u003e \u003cp\u003eRoutes of Entry 143\u003c\/p\u003e \u003cp\u003eMembrane Fusion 145\u003c\/p\u003e \u003cp\u003eIntracellular Trafficking and Uncoating 154\u003c\/p\u003e \u003cp\u003eMovement of Viral and Subviral Particles within Cells 154\u003c\/p\u003e \u003cp\u003eUncoating of Enveloped Virus Particles 155\u003c\/p\u003e \u003cp\u003eUncoating of Nonenveloped Viruses 155\u003c\/p\u003e \u003cp\u003eImport of Viral Genomes into the Nucleus 159\u003c\/p\u003e \u003cp\u003eThe Nuclear Pore Complex 159\u003c\/p\u003e \u003cp\u003eNuclear Localization Signals 159\u003c\/p\u003e \u003cp\u003eNuclear Import of RNA Genomes 161\u003c\/p\u003e \u003cp\u003eNuclear Import of DNA Genomes 162\u003c\/p\u003e \u003cp\u003eImport of Retroviral Genomes 162\u003c\/p\u003e \u003cp\u003ePerspectives 164\u003c\/p\u003e \u003cp\u003eReferences 165\u003c\/p\u003e \u003cp\u003eStudy Questions 166\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Synthesis of RNA from RNA Templates 168\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 169\u003c\/p\u003e \u003cp\u003eThe Nature of the RNA Template 169\u003c\/p\u003e \u003cp\u003eSecondary Structures in Viral RNA 169\u003c\/p\u003e \u003cp\u003eNaked or Nucleocapsid RNA 170\u003c\/p\u003e \u003cp\u003eThe RNA Synthesis Machinery 171\u003c\/p\u003e \u003cp\u003eIdentification of RNA-Dependent RNA Polymerases 171\u003c\/p\u003e \u003cp\u003eThree-Dimensional Structures of RNA-Dependent RNA Polymerases 173\u003c\/p\u003e \u003cp\u003eMechanisms of RNA Synthesis 176\u003c\/p\u003e \u003cp\u003eInitiation 176\u003c\/p\u003e \u003cp\u003eCapping 179\u003c\/p\u003e \u003cp\u003eElongation 179\u003c\/p\u003e \u003cp\u003eFunctions of Additional Polymerase Domains 181\u003c\/p\u003e \u003cp\u003eRNA Polymerase Oligomerization 181\u003c\/p\u003e \u003cp\u003eTemplate Specificity 182\u003c\/p\u003e \u003cp\u003eUnwinding the RNA Template 182\u003c\/p\u003e \u003cp\u003eRole of Cellular Proteins 183\u003c\/p\u003e \u003cp\u003eParadigms for Viral RNA Synthesis 183\u003c\/p\u003e \u003cp\u003e(+) Strand RNA 184\u003c\/p\u003e \u003cp\u003eSynthesis of Nested Subgenomic mRNAs 184\u003c\/p\u003e \u003cp\u003e(−) Strand RNA 185\u003c\/p\u003e \u003cp\u003eAmbisense RNA 189\u003c\/p\u003e \u003cp\u003eDouble-Stranded RNA 189\u003c\/p\u003e \u003cp\u003eUnique Mechanisms of mRNA and Genome Synthesis of Hepatitis Delta Virus 190\u003c\/p\u003e \u003cp\u003eDo Ribosomes and RNA Polymerases Collide? 192\u003c\/p\u003e \u003cp\u003eOrigins of Diversity in RNA Virus Genomes 193\u003c\/p\u003e \u003cp\u003eMisincorporation of Nucleotides 193\u003c\/p\u003e \u003cp\u003eSegment Reassortment and RNA Recombination 193\u003c\/p\u003e \u003cp\u003eRNA Editing 194\u003c\/p\u003e \u003cp\u003ePerspectives 195\u003c\/p\u003e \u003cp\u003eReferences 196\u003c\/p\u003e \u003cp\u003eStudy Questions 197\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Synthesis of RNA from DNA Templates 198\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 199\u003c\/p\u003e \u003cp\u003eProperties of Cellular RNA Polymerases That Transcribe Viral DNA 199\u003c\/p\u003e \u003cp\u003eSome Viral Genomes Must Be Converted to Templates Suitable for Transcription 200\u003c\/p\u003e \u003cp\u003eTranscription by RNA Polymerase II 201\u003c\/p\u003e \u003cp\u003eRegulation of RNA Polymerase II Transcription 203\u003c\/p\u003e \u003cp\u003eCommon Properties of Proteins That Regulate Transcription 206\u003c\/p\u003e \u003cp\u003eTranscription of Viral DNA Templates by the Cellular Machinery Alone 208\u003c\/p\u003e \u003cp\u003eViral Proteins That Govern Transcription of DNA Templates 209\u003c\/p\u003e \u003cp\u003ePatterns of Regulation 209\u003c\/p\u003e \u003cp\u003eThe Human Immunodeficiency Virus Type 1 Tat Protein Autoregulates Transcription 211\u003c\/p\u003e \u003cp\u003eThe Transcriptional Cascades of DNA Viruses 217\u003c\/p\u003e \u003cp\u003eEntry into One of Two Alternative Transcriptional Programs 226\u003c\/p\u003e \u003cp\u003eTranscription of Viral Genes by RNA Polymerase III 230\u003c\/p\u003e \u003cp\u003eThe VA-RNA I Promoter 231\u003c\/p\u003e \u003cp\u003eInhibition of the Cellular Transcriptional Machinery 232\u003c\/p\u003e \u003cp\u003eUnusual Functions of Cellular Transcription Components in Virus-Infected Cells 233\u003c\/p\u003e \u003cp\u003eViral DNA-Dependent RNA Polymerases 233\u003c\/p\u003e \u003cp\u003ePerspectives 234\u003c\/p\u003e \u003cp\u003eReferences 235\u003c\/p\u003e \u003cp\u003eStudy Questions 236\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Processing 238\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 239\u003c\/p\u003e \u003cp\u003eCovalent Modification during Viral Pre-mRNA Processing 240\u003c\/p\u003e \u003cp\u003eCapping the 5′ Ends of Viral mRNA 240\u003c\/p\u003e \u003cp\u003eSynthesis of 3′ Poly(A) Segments of Viral mRNA 243\u003c\/p\u003e \u003cp\u003eInternal Methylation of Adenosine Residues 245\u003c\/p\u003e \u003cp\u003eSplicing of Viral Pre-mRNA 246\u003c\/p\u003e \u003cp\u003eRegulated Processing of Viral Pre-mRNA 249\u003c\/p\u003e \u003cp\u003eEditing of Viral mRNAs 255\u003c\/p\u003e \u003cp\u003eExport of RNAs from the Nucleus 257\u003c\/p\u003e \u003cp\u003eThe Cellular Export Machinery 257\u003c\/p\u003e \u003cp\u003eExport of Viral mRNA 258\u003c\/p\u003e \u003cp\u003ePosttranscriptional Regulation of Viral or Cellular Gene Expression by Viral Proteins 262\u003c\/p\u003e \u003cp\u003eTemporal Control of Viral Gene Expression 262\u003c\/p\u003e \u003cp\u003eViral Proteins Can Inhibit Cellular mRNA Production 264\u003c\/p\u003e \u003cp\u003eRegulation of Turnover of Viral and Cellular mRNAs in the Cytoplasm 266\u003c\/p\u003e \u003cp\u003eIntrinsic Turnover 266\u003c\/p\u003e \u003cp\u003eRegulation of mRNA Stability by Viral Proteins 267\u003c\/p\u003e \u003cp\u003emRNA Stabilization Can Facilitate Transformation 267\u003c\/p\u003e \u003cp\u003eNonsense-Mediated mRNA Decay 267\u003c\/p\u003e \u003cp\u003eNoncoding RNAs 271\u003c\/p\u003e \u003cp\u003eSmall Interfering RNAs and Micro-RNAs 271\u003c\/p\u003e \u003cp\u003eLong Noncoding RNAs 276\u003c\/p\u003e \u003cp\u003eCircular RNAs 278\u003c\/p\u003e \u003cp\u003ePerspectives 278\u003c\/p\u003e \u003cp\u003eReferences 279\u003c\/p\u003e \u003cp\u003eStudy Questions 281\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Replication of DNA Genomes 282\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 283\u003c\/p\u003e \u003cp\u003eDNA Synthesis by the Cellular Replication Machinery 284\u003c\/p\u003e \u003cp\u003eEukaryotic Replicons 284\u003c\/p\u003e \u003cp\u003eCellular Replication Proteins 287\u003c\/p\u003e \u003cp\u003eMechanisms of Viral DNA Synthesis 287\u003c\/p\u003e \u003cp\u003eLessons from Simian Virus 40 288\u003c\/p\u003e \u003cp\u003eReplication of Other Viral DNA Genomes 290\u003c\/p\u003e \u003cp\u003eProperties of Viral Replication Origins 294\u003c\/p\u003e \u003cp\u003eRecognition of Viral Replication Origins 296\u003c\/p\u003e \u003cp\u003eViral DNA Synthesis Machines 301\u003c\/p\u003e \u003cp\u003eResolution and Processing of Viral Replication Products 301\u003c\/p\u003e \u003cp\u003eExponential Accumulation of Viral Genomes 302\u003c\/p\u003e \u003cp\u003eViral Proteins Can Induce Synthesis of Cellular Replication Proteins 303\u003c\/p\u003e \u003cp\u003eSynthesis of Viral Replication Machines and Accessory Enzymes 304\u003c\/p\u003e \u003cp\u003eViral DNA Replication Independent of Cellular Proteins 304\u003c\/p\u003e \u003cp\u003eDelayed Synthesis of Structural Proteins Prevents Premature Packaging of DNA Templates 305\u003c\/p\u003e \u003cp\u003eInhibition of Cellular DNA Synthesis 305\u003c\/p\u003e \u003cp\u003eSynthesis of Viral DNA in Specialized Intracellular Compartments 305\u003c\/p\u003e \u003cp\u003eLimited Replication of Viral DNA Genomes 308\u003c\/p\u003e \u003cp\u003eIntegrated Parvoviral DNA Can Be Replicated as Part of the Cellular Genome 308\u003c\/p\u003e \u003cp\u003eDifferent Viral Origins Regulate Replication of Epstein-Barr Virus 310\u003c\/p\u003e \u003cp\u003eLimited and Amplifying Replication from a Single Origin: the Papillomaviruses 313\u003c\/p\u003e \u003cp\u003eOrigins of Genetic Diversity in DNA Viruses 315\u003c\/p\u003e \u003cp\u003eFidelity of Replication by Viral DNA Polymerases 315\u003c\/p\u003e \u003cp\u003eModulation of the DNA Damage Response 316\u003c\/p\u003e \u003cp\u003eRecombination of Viral Genomes 318\u003c\/p\u003e \u003cp\u003ePerspectives 321\u003c\/p\u003e \u003cp\u003eReferences 321\u003c\/p\u003e \u003cp\u003eStudy Questions 323\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Reverse Transcription and Integration 324\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eRetroviral Reverse Transcription 325\u003c\/p\u003e \u003cp\u003eDiscovery 325\u003c\/p\u003e \u003cp\u003eImpact 325\u003c\/p\u003e \u003cp\u003eThe Process of Reverse Transcription 326\u003c\/p\u003e \u003cp\u003eGeneral Properties and Structure of Retroviral Reverse Transcriptases 334\u003c\/p\u003e \u003cp\u003eOther Examples of Reverse Transcription 337\u003c\/p\u003e \u003cp\u003eRetroviral DNA Integration 340\u003c\/p\u003e \u003cp\u003eThe Pathway of Integration: Integrase-Catalyzed Steps 341\u003c\/p\u003e \u003cp\u003eIntegrase Structure and Mechanism 347\u003c\/p\u003e \u003cp\u003eHepadnaviral Reverse Transcription 350\u003c\/p\u003e \u003cp\u003eA DNA Virus with Reverse Transcriptase 350\u003c\/p\u003e \u003cp\u003eThe Process of Hepadnaviral Reverse Transcription 352\u003c\/p\u003e \u003cp\u003ePerspectives 358\u003c\/p\u003e \u003cp\u003eReferences 359\u003c\/p\u003e \u003cp\u003eStudy Questions 360\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Protein Synthesis 362\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 363\u003c\/p\u003e \u003cp\u003eMechanisms of Eukaryotic Protein Synthesis 363\u003c\/p\u003e \u003cp\u003eGeneral Structure of Eukaryotic mRNA 363\u003c\/p\u003e \u003cp\u003eThe Translation Machinery 364\u003c\/p\u003e \u003cp\u003eInitiation 365\u003c\/p\u003e \u003cp\u003eElongation and Termination 375\u003c\/p\u003e \u003cp\u003eThe Diversity of Viral Translation Strategies 378\u003c\/p\u003e \u003cp\u003ePolyprotein Synthesis 378\u003c\/p\u003e \u003cp\u003eLeaky Scanning 378\u003c\/p\u003e \u003cp\u003eReinitiation 381\u003c\/p\u003e \u003cp\u003eStopGo Translation 382\u003c\/p\u003e \u003cp\u003eSuppression of Termination 382\u003c\/p\u003e \u003cp\u003eRibosomal Frameshifting 383\u003c\/p\u003e \u003cp\u003eBicistronic mRNAs 384\u003c\/p\u003e \u003cp\u003eRegulation of Translation during Viral Infection 385\u003c\/p\u003e \u003cp\u003eInhibition of Translation Initiation after Viral Infection 385\u003c\/p\u003e \u003cp\u003eRegulation of eIF4F 389\u003c\/p\u003e \u003cp\u003eRegulation of Poly(A)-Binding Protein Activity 392\u003c\/p\u003e \u003cp\u003eRegulation of eIF3 392\u003c\/p\u003e \u003cp\u003eInterfering with RNA 392\u003c\/p\u003e \u003cp\u003eStress-Associated RNA Granules 393\u003c\/p\u003e \u003cp\u003ePerspectives 395\u003c\/p\u003e \u003cp\u003eReferences 396\u003c\/p\u003e \u003cp\u003eStudy Questions 397\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Intracellular Trafficking 398\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 399\u003c\/p\u003e \u003cp\u003eAssembly within the Nucleus 400\u003c\/p\u003e \u003cp\u003eImport of Viral Proteins for Assembly 401\u003c\/p\u003e \u003cp\u003eAssembly at the Plasma Membrane 403\u003c\/p\u003e \u003cp\u003eTransport of Viral Membrane Proteins to the Plasma Membrane 404\u003c\/p\u003e \u003cp\u003eSorting of Viral Proteins in Polarized Cells 419\u003c\/p\u003e \u003cp\u003eDisruption of the Secretory Pathway in Virus-Infected Cells 421\u003c\/p\u003e \u003cp\u003eSignal Sequence-Independent Transport of Viral Proteins to the Plasma Membrane 422\u003c\/p\u003e \u003cp\u003eInteractions with Internal Cellular Membranes 426\u003c\/p\u003e \u003cp\u003eLocalization of Viral Proteins to Compartments of the Secretory Pathway 426\u003c\/p\u003e \u003cp\u003eLocalization of Viral Proteins to the Nuclear Membrane 426\u003c\/p\u003e \u003cp\u003eTransport of Viral Genomes to Assembly Sites 427\u003c\/p\u003e \u003cp\u003eTransport of Genomic and Pregenomic RNA from the Nucleus to the Cytoplasm 427\u003c\/p\u003e \u003cp\u003eTransport of Genomes from the Cytoplasm to the Plasma Membrane 429\u003c\/p\u003e \u003cp\u003ePerspectives 430\u003c\/p\u003e \u003cp\u003eReferences 431\u003c\/p\u003e \u003cp\u003eStudy Questions 432\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Assembly, Release, and Maturation 434\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 435\u003c\/p\u003e \u003cp\u003eMethods of Studying Virus Assembly and Egress 435\u003c\/p\u003e \u003cp\u003eStructural Studies of Virus Particles 436\u003c\/p\u003e \u003cp\u003eVisualization of Assembly and Exit by Microscopy 436\u003c\/p\u003e \u003cp\u003eBiochemical and Genetic Analyses of Assembly Intermediates 436\u003c\/p\u003e \u003cp\u003eMethods Based on Recombinant DNA Technology 439\u003c\/p\u003e \u003cp\u003eAssembly of Protein Shells 439\u003c\/p\u003e \u003cp\u003eFormation of Structural Units 439\u003c\/p\u003e \u003cp\u003eCapsid and Nucleocapsid Assembly 441\u003c\/p\u003e \u003cp\u003eSelf-Assembly and Assisted Assembly Reactions 445\u003c\/p\u003e \u003cp\u003eSelective Packaging of the Viral Genome and Other Components of Virus Particles 447\u003c\/p\u003e \u003cp\u003eConcerted or Sequential Assembly 447\u003c\/p\u003e \u003cp\u003eRecognition and Packaging of the Nucleic Acid Genome 448\u003c\/p\u003e \u003cp\u003eIncorporation of Enzymes and Other Nonstructural Proteins 458\u003c\/p\u003e \u003cp\u003eAcquisition of an Envelope 459\u003c\/p\u003e \u003cp\u003eSequential Assembly of Internal Components and Budding from a Cellular Membrane 459\u003c\/p\u003e \u003cp\u003eCoordination of the Assembly of Internal Structures with Acquisition of the Envelope 460\u003c\/p\u003e \u003cp\u003eRelease of Virus Particles 460\u003c\/p\u003e \u003cp\u003eAssembly and Budding at the Plasma Membrane 461\u003c\/p\u003e \u003cp\u003eAssembly at Internal Membranes: the Problem of Exocytosis 464\u003c\/p\u003e \u003cp\u003eRelease of Nonenveloped Virus Particles 470\u003c\/p\u003e \u003cp\u003eMaturation of Progeny Virus Particles 470\u003c\/p\u003e \u003cp\u003eProteolytic Processing of Structural Proteins 470\u003c\/p\u003e \u003cp\u003eOther Maturation Reactions 474\u003c\/p\u003e \u003cp\u003eCell-to-Cell Spread 475\u003c\/p\u003e \u003cp\u003ePerspectives 479\u003c\/p\u003e \u003cp\u003eReferences 479\u003c\/p\u003e \u003cp\u003eStudy Questions 481\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 The Infected Cell 482\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 483\u003c\/p\u003e \u003cp\u003eSignal Transduction 483\u003c\/p\u003e \u003cp\u003eSignaling Pathways 483\u003c\/p\u003e \u003cp\u003eSignaling in Virus-Infected Cells 485\u003c\/p\u003e \u003cp\u003eGene Expression 489\u003c\/p\u003e \u003cp\u003eInhibition of Cellular Gene Expression 489\u003c\/p\u003e \u003cp\u003eDifferential Regulation of Cellular Gene Expression 492\u003c\/p\u003e \u003cp\u003eMetabolism 496\u003c\/p\u003e \u003cp\u003eMethods To Study Metabolism 496\u003c\/p\u003e \u003cp\u003eGlucose Metabolism 497\u003c\/p\u003e \u003cp\u003eThe Citric Acid Cycle 501\u003c\/p\u003e \u003cp\u003eElectron Transport and Oxidative Phosphorylation 502\u003c\/p\u003e \u003cp\u003eLipid Metabolism 504\u003c\/p\u003e \u003cp\u003eRemodeling of Cellular Organelles 507\u003c\/p\u003e \u003cp\u003eThe Nucleus 509\u003c\/p\u003e \u003cp\u003eThe Cytoplasm 511\u003c\/p\u003e \u003cp\u003ePerspectives 516\u003c\/p\u003e \u003cp\u003eReferences 518\u003c\/p\u003e \u003cp\u003eStudy Questions 519\u003c\/p\u003e \u003cp\u003eAppendix Structure, Genome Organization, and Infectious Cycles of Viruses Featured in This Book 521\u003c\/p\u003e \u003cp\u003eGlossary 557\u003c\/p\u003e \u003cp\u003eIndex 563\u003c\/p\u003e \u003cp\u003eJane Flint is Professor Emerita of Molecular Biology at Princeton University. Dr. Flint’s research focused on investigation of the mechanisms by which viral gene products modulate host pathways and antiviral defenses to allow efficient reproduction in normal human cells of adenoviruses, viruses that are used in such therapeutic applications as gene transfer and cancer treatment.\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003eVincent R. Racaniello is Higgins Professor of Microbiology \u0026amp; Immunology at Columbia University Vagelos College of Physicians \u0026amp; Surgeons. Dr. Racaniello has been studying viruses for over 40 years, including polio- virus, rhinovirus, enteroviruses, hepatitis C virus, and Zika virus. He blogs about virus-es at virology.ws and is host of This Week in Virology.\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003eGlenn F. Rall is a Professor and the Chief Academic Officer at the Fox Chase Cancer Center, and is an Adjunct Professor in the Microbiology and Immunology departments at the University of Pennsylvania, as well as Thomas Jefferson, Drexel, and Temple Universities. Dr. Rall studies viral infections of the brain and the immune responses to those infections, with the goal of defining how viruses contribute to disease.\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003eTheodora Hatziioannou is a Research Associate Professor at Rockefeller University and is actively involved in teaching programs at Albert Einstein College of Medicine. Dr. Hatziioannou has worked on multiple viruses with a focus on retroviruses and the molecular mechanisms that govern virus tropism and on the improvement of animal models for human disease.\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003eAnna Marie Skalka is a Professor Emerita and former Senior Vice President for Basic Research at the Fox Chase Cancer Center. Dr. Skalka is internationally recognized for her contributions to the understanding of the biochemical mechanisms by which retroviruses replicate and insert their genetic material into the host genome, as well as her research into other molecular aspects of retrovirus biology.\u003c\/p\u003e \u003cp\u003e\u003ci\u003ePrinciples of Virology\u003c\/i\u003e, the leading virology textbook in use, is an extremely valuable and highly informative presentation of virology at the interface of modern cell biology and immunology. This text utilizes a uniquely rational approach by highlighting common principles and processes across all viruses. Using a set of representative viruses to illustrate the breadth of viral complexity, students are able to under-stand viral reproduction and pathogenesis and are equipped with the necessary tools for future encounters with new or understudied viruses.\u003cbr\u003e \u003cbr\u003e This fifth edition was updated to keep pace with the ever-changing field of virology. In addition to the beloved full-color illustrations, video interviews with leading scientists, movies, and links to exciting blogposts on relevant topics, this edition includes study questions and active learning puzzles in each chapter, as well as short descriptions regarding the key messages of references of special interest. \u003cbr\u003e \u003cbr\u003e \u003ci\u003eVolume I: Molecular Biology\u003c\/i\u003e focuses on the molecular processes of viral reproduction, from entry through release. \u003ci\u003eVolume II: Pathogenesis and Control\u003c\/i\u003e addresses the interplay between viruses and their host organisms, on both the micro- and macroscale, including chapters on public health, the immune response, vaccines and other antiviral strategies, viral evolution, and a brand new chapter on the therapeutic uses of viruses. These two volumes can be used for separate courses or together in a single course. Each includes a unique appendix, glossary, and links to internet resources.\u003cbr\u003e \u003cbr\u003e \u003ci\u003ePrinciples of Virology, Fifth Edition\u003c\/i\u003e, is ideal for teaching the strategies by which all viruses reproduce, spread within a host, and are maintained within populations. This edition carefully reflects the results of extensive vetting and feedback received from course instructors and students, making this renowned textbook even more appropriate for undergraduate and graduate courses in virology, microbiology, and infectious diseases.\u003c\/p\u003e","brand":"ASM Press","offers":[{"title":"Default Title","offer_id":47989855125733,"sku":"NP9781683672845","price":84.5,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9781683672845.jpg?v=1761785687","url":"https:\/\/k12savings.com\/products\/principles-of-virology-volume-1-isbn-9781683672845","provider":"K12savings","version":"1.0","type":"link"}