{"product_id":"primary-and-stem-cells-isbn-9780470610749","title":"Primary and Stem Cells","description":"This book describes basic cell engineering methods, emphasizing stem cell applications, and use of the genetically modified stem cells in cell therapy and drug discovery. Together, the chapters introduce and offer insights on new techniques for engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems. The book offers a guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose, whether it's basic science investigation, creation of disease models and screens, or cells for therapeutic applications. \u003cp\u003eForeword \u003ci\u003eMichele Calos\u003c\/i\u003e vii\u003c\/p\u003e \u003cp\u003ePreface ix\u003c\/p\u003e \u003cp\u003eContributors xi\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart I: Cloning and Gene Delivery 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1. DNA Assembly Technologies Based on Homologous Recombination 3\u003cbr\u003e \u003ci\u003eBillyana Tsvetanova, Lansha Peng, Xiquan Liang, Ke Li, Jian-Ping Yang, Tony Ho, Josh Shirley, Liewei Xu, Jason Potter, Wieslaw Kudlicki, Todd Peterson, and Federico Katzen\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2. Multigene Assembly for Construction of Synthetic Operons: Creation and Delivery of an Optimized All-IN-One Expression Construct for Generating Mouse iPS Cells 19\u003cbr\u003e \u003ci\u003eTakefumi Sone, Yoko Takata, Hiroe Kishine, Taichi Andoh, Fumio Imamoto, and Jonathan D. Chesnut\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3. Strategies for the Delivery of Naked DNA 37\u003cbr\u003e \u003ci\u003eJennifer C. Moore and Ronald P. Hart\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart II: Nonintegrating Technologies 49\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4. Episomal Vectors 51\u003cbr\u003e \u003ci\u003eJunying Yu\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5. Nonintegrating DNA Virus 85\u003cbr\u003e \u003ci\u003eChris Kemp and David Kuninger\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6. Nonintegrating RNA Viruses 103\u003cbr\u003e \u003ci\u003eNoemi Fusaki\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7. Protein Delivery 119\u003cbr\u003e \u003ci\u003eXiao-Jian Han and Kazuhito Tomizawa\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart III: Integrating Technologies 135\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8. Sleeping Beauty Transposon-Mediated Stable Gene Delivery 137\u003cbr\u003e \u003ci\u003eAndrew Wilber, Xianzheng Zhou, Xin Huang, Dan S. Kaufman, and R. Scott McIvor\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e9. Integrating Viral Vectors for Gene Modifications 163\u003cbr\u003e \u003ci\u003eGemma L. Mendel, Brian Paszkiet, and Rafal P. Witek\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e10. Bacteriophage Integrases for Site-Specific Integration 199\u003cbr\u003e \u003ci\u003eBhaskar Thyagarajan\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e11. Improving Gene Targeting Efficiency in Human Pluripotent Stem Cells 211\u003cbr\u003e \u003ci\u003eSangyoon Han, Anna McCann, Louise C. Laurent, Jeanne F. Loring, and Ying Liu\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart IV: Applications 227\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e12. Modified Stem Cells as Disease Models and in Toxicology Screening 229\u003cbr\u003e \u003ci\u003eCatharina Ellerstr€om, Peter Sartipy, Raimund Strehl, and Johan Hyllner\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e13. Screening and Drug Discovery 251\u003cbr\u003e \u003ci\u003eElizabeth A. Davenport and Robert S. Ames\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eIndex 273\u003c\/p\u003e  \u003cp\u003e“This is an excellent resource on the new techniques for the engineering of stem cells and the delivery of transgenes into stem cells via various viral and nonviral systems. It provides comprehensive information on the concepts of cell engineering principles and their application in primary and stem cells.  Researchers, established stem cell scientists, and graduate students interested in this field will find this book very handy.”  (\u003ci\u003eDoody’s\u003c\/i\u003e, 17 August 2012)\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e  \u003cb\u003eUMA LAKSHMIPATHY\u003c\/b\u003e is a principal investigator at Life Technologies. She has a PhD in life sciences, with academic and industry experience in molecular biology and stem cells. Dr. Lakshmipathy holds four patents and has authored more than forty publications.  \u003cp\u003e\u003cb\u003eBHASKAR THYAGARAJAN\u003c\/b\u003e is a program manager at Life Technologies. He has a PhD in pharmacology, with expertise in the areas of molecular biology, DNA recombination, gene and cell therapy, and protein purification. He holds one patent and has authored more than twenty publications.\u003c\/p\u003e  \u003cb\u003eThe all-important resource that lays the groundwork for researchers to genetically modify primary and stem cells for use in downstream applications\u003c\/b\u003e  \u003cp\u003eReplacing damaged or lost organs by producing \"spare parts\" is a concept that lies at the core of the varied biotechnological practices generally referred to as tissue engineering. An important component in this regeneration process includes the use of stem cells, which harbor the ability to mature into other cell types and hold the potential to act as a repair system for the body. \u003ci\u003ePrimary and Stem Cells\u003c\/i\u003e describes basic cell engineering methods, emphasizing stem cell applications, and the use of genetically modified stem cells in cell therapy and drug discovery. Discussion includes new techniques for the engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems, along with:\u003c\/p\u003e \u003cul\u003e \u003cli\u003e \u003cp\u003eTransfection techniques and methods for cell line engineering\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eInformation that assists researchers and drug developers in deciding how to generate their required drug or gene therapists in developing genetically modified cells\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eAn overview of applications in areas like disease models and drug discovery\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eIssues that address the interests of companies involved in stem cell tools and reagents\u003c\/p\u003e \u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003ci\u003ePrimary and Stem Cells\u003c\/i\u003e serves as an essential guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose —basic science investigation, creation of disease models and screens, or cells for therapeutic applications—and acts as the launching pad for pioneering further development in a field emerging with exciting possibilities.\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47989847752933,"sku":"NP9780470610749","price":142.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9780470610749.jpg?v=1761785660","url":"https:\/\/k12savings.com\/products\/primary-and-stem-cells-isbn-9780470610749","provider":"K12savings","version":"1.0","type":"link"}