{"product_id":"practical-high-performance-liquid-chromatography-isbn-9780470682180","title":"Practical High-Performance Liquid Chromatography","description":"\u003cb\u003eJump into the HPLC adventure!\u003c\/b\u003e \u003cp\u003eThree decades on from publication of the 1st German edition of Veronika Meyer's book on HPLC, this classic text remains one of the few titles available on general HPLC aimed at practitioners.\u003c\/p\u003e \u003cp\u003eNew sections on the following topics have been included in this fifth edition:\u003c\/p\u003e \u003cul\u003e \u003cli\u003eComparison of HPLC with capillary electrophoresis\u003c\/li\u003e \u003cli\u003eHow to obtain peak capacity\u003c\/li\u003e \u003cli\u003evan Deemter curves and other coherences\u003c\/li\u003e \u003cli\u003eHydrophilic interaction chromatography\u003c\/li\u003e \u003cli\u003eMethod transfer\u003c\/li\u003e \u003cli\u003eComprehensive two-dimensional HPLC\u003c\/li\u003e \u003cli\u003eFast separations at 1000 bar\u003c\/li\u003e \u003cli\u003eHPLC with superheated water\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eIn addition, two chapters on the instrument test and troubleshooting in the appendix have been updated and expanded by Bruno E. Lendi, and many details have been improved and numerous references added.\u003c\/p\u003e \u003cp\u003eA completely new chapter is presented on quality assurance covering:\u003c\/p\u003e \u003cul\u003e \u003cli\u003eIs it worth the effort?\u003c\/li\u003e \u003cli\u003eVerification with a second method\u003c\/li\u003e \u003cli\u003eMethod validation\u003c\/li\u003e \u003cli\u003eStandard operating procedures\u003c\/li\u003e \u003cli\u003eMeasurement uncertainty\u003c\/li\u003e \u003cli\u003eQualifications, instrument test, and system suitability test\u003c\/li\u003e \u003cli\u003eThe quest for quality\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003cb\u003eReviews of earlier editions\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\"That this text is written by an expert in both the practice and teaching of HPLC is evident from the first paragraph....not only an enjoyable, fascinating and easy read, but a truly excellent text that has and will serve many teachers, students and practitioners very well.\" —\u003ci\u003eThe Analyst\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e“…provides essential information on HPLC for LC practitioners in academia, industry, government, and research laboratories…a valuable introduction.\" - \u003ci\u003eAmerican Journal of Therapeutics\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003ePreface to the Fifth Edition xiii\u003c\/p\u003e \u003cp\u003eImportant and Useful Equations for HPLC 1 vii\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Introduction 5\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 HPLC: A powerful separation method 5\u003c\/p\u003e \u003cp\u003e1.2 A first HPLC experiment 5\u003c\/p\u003e \u003cp\u003e1.3 Liquid chromatographic separation modes 8\u003c\/p\u003e \u003cp\u003e1.4 The HPLC instrument 9\u003c\/p\u003e \u003cp\u003e1.5 Safety in the HPLC laboratory 10\u003c\/p\u003e \u003cp\u003e1.6 Comparison between high-performance liquid chromatography and gas chromatography 11\u003c\/p\u003e \u003cp\u003e1.7 Comparison between high-performance liquid chromatography and capillary electrophoresis 12\u003c\/p\u003e \u003cp\u003e1.8 Units for pressure, length and viscosity 13\u003c\/p\u003e \u003cp\u003e1.9 Scientific journals 14\u003c\/p\u003e \u003cp\u003e1.10 Recommended books 15\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Theoretical Principles 17\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 The chromatographic process 17\u003c\/p\u003e \u003cp\u003e2.2 Band broadening 19\u003c\/p\u003e \u003cp\u003e2.3 The chromatogram and its purport 23\u003c\/p\u003e \u003cp\u003e2.4 Graphical representation of peak pairs with different degree of resolution 30\u003c\/p\u003e \u003cp\u003e2.5 Factors affecting resolution 35\u003c\/p\u003e \u003cp\u003e2.6 Extra-column volumes (dead volumes) 40\u003c\/p\u003e \u003cp\u003e2.7 Tailing 41\u003c\/p\u003e \u003cp\u003e2.8 Peak capacity and statistical resolution probability 46\u003c\/p\u003e \u003cp\u003e2.9 Effects of temperature in HPLC 49\u003c\/p\u003e \u003cp\u003e2.10 The limits of HPLC 51\u003c\/p\u003e \u003cp\u003e2.11 How to obtain peak capacity 55\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Pumps 59\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 General requirements 59\u003c\/p\u003e \u003cp\u003e3.2 The short-stroke piston pump 59\u003c\/p\u003e \u003cp\u003e3.3 Maintenance and repair 62\u003c\/p\u003e \u003cp\u003e3.4 Other pump designs 63\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Preparation of Equipment up to Sample Injection 65\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Selection of the mobile phase 65\u003c\/p\u003e \u003cp\u003e4.2 Preparation of the mobile phase 67\u003c\/p\u003e \u003cp\u003e4.3 Gradient systems 68\u003c\/p\u003e \u003cp\u003e4.4 Capillary tubing 70\u003c\/p\u003e \u003cp\u003e4.5 Fittings 72\u003c\/p\u003e \u003cp\u003e4.6 Sample injectors 74\u003c\/p\u003e \u003cp\u003e4.7 Sample solution and sample volume 78\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Solvent Properties 81\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Table of organic solvents 81\u003c\/p\u003e \u003cp\u003e5.2 Solvent selectivity 83\u003c\/p\u003e \u003cp\u003e5.3 Miscibility 83\u003c\/p\u003e \u003cp\u003e5.4 Buffers 84\u003c\/p\u003e \u003cp\u003e5.5 Shelf life of mobile phases. 87\u003c\/p\u003e \u003cp\u003e5.6 The mixing cross 88\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Detectors 91\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 General 91\u003c\/p\u003e \u003cp\u003e6.2 UV detectors 96\u003c\/p\u003e \u003cp\u003e6.3 Refractive index detectors 99\u003c\/p\u003e \u003cp\u003e6.4 Fluorescence detectors 101\u003c\/p\u003e \u003cp\u003e6.5 Electrochemical (amperometric) detectors 103\u003c\/p\u003e \u003cp\u003e6.6 Light-scattering detectors 104\u003c\/p\u003e \u003cp\u003e6.7 Other detectors 106\u003c\/p\u003e \u003cp\u003e6.8 Multiple detection 107\u003c\/p\u003e \u003cp\u003e6.9 Indirect detection 108\u003c\/p\u003e \u003cp\u003e6.10 Coupling with spectroscopy 109\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Columns and Stationary Phases 117\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Columns for HPLC 117\u003c\/p\u003e \u003cp\u003e7.2 Precolumns 119\u003c\/p\u003e \u003cp\u003e7.3 General properties of stationary phases 120\u003c\/p\u003e \u003cp\u003e7.4 Silica 125\u003c\/p\u003e \u003cp\u003e7.5 Chemically modified silica 126\u003c\/p\u003e \u003cp\u003e7.6 Styrene-divinylbenzene 129\u003c\/p\u003e \u003cp\u003e7.7 Some other stationary phases 133\u003c\/p\u003e \u003cp\u003e7.8 Column care and regeneration 136\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 HPLC Column Tests 141\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 Simple tests for HPLC columns 141\u003c\/p\u003e \u003cp\u003e8.2 Determination of particle size 143\u003c\/p\u003e \u003cp\u003e8.3 Determination of breakthrough time 144\u003c\/p\u003e \u003cp\u003e8.4 The test mixture 146\u003c\/p\u003e \u003cp\u003e8.5 Dimensionless parameters for HPLC column characterization 148\u003c\/p\u003e \u003cp\u003e8.6 The van Deemter equation from reduced parameters and its use in column diagnosis 152\u003c\/p\u003e \u003cp\u003e8.7 van Deemter curves and other coherences 153\u003c\/p\u003e \u003cp\u003e8.8 Diffusion coefficients 155\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Adsorption Chromatography: Normal-Phase Chromatography 159\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 What is adsorption? 159\u003c\/p\u003e \u003cp\u003e9.2 The eluotropic series 162\u003c\/p\u003e \u003cp\u003e9.3 Selectivity properties of the mobile phase 165\u003c\/p\u003e \u003cp\u003e9.4 Choice and optimization of the mobile phase 166\u003c\/p\u003e \u003cp\u003e9.5 Applications 168\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Reversed-Phase Chromatography 173\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e10.1 Principle 173\u003c\/p\u003e \u003cp\u003e10.2 Mobile phases in reversed-phase chromatography 174\u003c\/p\u003e \u003cp\u003e10.3 Solvent selectivity and strength 177\u003c\/p\u003e \u003cp\u003e10.4 Stationary phases 181\u003c\/p\u003e \u003cp\u003e10.5 Method development in reversed-phase chromatography 185\u003c\/p\u003e \u003cp\u003e10.6 Applications 188\u003c\/p\u003e \u003cp\u003e10.7 Hydrophobic interaction chromatography 191\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Chromatography with Chemically Bonded Phases 195\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e11.1 Introduction 195\u003c\/p\u003e \u003cp\u003e11.2 Properties of some stationary phases 195\u003c\/p\u003e \u003cp\u003e11.3 Hydrophilic interaction chromatography 200\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Ion-Exchange Chromatography 203\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction 203\u003c\/p\u003e \u003cp\u003e12.2 Principle 203\u003c\/p\u003e \u003cp\u003e12.3 Properties of ion exchangers 204\u003c\/p\u003e \u003cp\u003e12.4 Influence of the mobile phase 207\u003c\/p\u003e \u003cp\u003e12.5 Special possibilities of ion exchange 208\u003c\/p\u003e \u003cp\u003e12.6 Practical hints 210\u003c\/p\u003e \u003cp\u003e12.7 Applications 213\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Ion-Pair Chromatography 217\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e13.1 Introduction 217\u003c\/p\u003e \u003cp\u003e13.2 Ion-pair chromatography in practice 218\u003c\/p\u003e \u003cp\u003e13.3 Applications 220\u003c\/p\u003e \u003cp\u003e13.4 Appendix: UV detection using ion-pair reagents 221\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 Ion Chromatography 225\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e14.1 Principle 225\u003c\/p\u003e \u003cp\u003e14.2 Suppression techniques 226\u003c\/p\u003e \u003cp\u003e14.3 Phase systems 226\u003c\/p\u003e \u003cp\u003e14.4 Applications 230\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 Size-Exclusion Chromatography 231\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e15.1 Principle 231\u003c\/p\u003e \u003cp\u003e15.2 The calibration chromatogram 234\u003c\/p\u003e \u003cp\u003e15.3 Molecular mass determination by means of size-exclusion chromatography 238\u003c\/p\u003e \u003cp\u003e15.4 Coupled size-exclusion columns 241\u003c\/p\u003e \u003cp\u003e15.5 Phase systems 243\u003c\/p\u003e \u003cp\u003e15.6 Applications 244\u003c\/p\u003e \u003cp\u003e\u003cb\u003e16 Affinity Chromatography. 249\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e16.1 Principle 249\u003c\/p\u003e \u003cp\u003e16.2 Affinity chromatography as a special case of HPLC 251\u003c\/p\u003e \u003cp\u003e16.3 Applications 252\u003c\/p\u003e \u003cp\u003e\u003cb\u003e17 Choice of Method 255\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e17.1 The various possibilities 255\u003c\/p\u003e \u003cp\u003e17.2 Method transfer 260\u003c\/p\u003e \u003cp\u003e\u003cb\u003e18 Solving the Elution Problem 263\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e18.1 The elution problem 263\u003c\/p\u003e \u003cp\u003e18.2 Solvent gradients 264\u003c\/p\u003e \u003cp\u003e18.3 Column switching 270\u003c\/p\u003e \u003cp\u003e18.4 Comprehensive two-dimensional HPLC 272\u003c\/p\u003e \u003cp\u003e18.5 Optimization of an isocratic chromatogram using four solvents 273\u003c\/p\u003e \u003cp\u003e18.6 Optimization of the other parameters 276\u003c\/p\u003e \u003cp\u003e18.7 Mixed stationary phases 284\u003c\/p\u003e \u003cp\u003e\u003cb\u003e19 Analytical HPLC 285\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e19.1 Qualitative analysis 285\u003c\/p\u003e \u003cp\u003e19.2 Trace analysis 287\u003c\/p\u003e \u003cp\u003e19.3 Quantitative analysis 291\u003c\/p\u003e \u003cp\u003e19.4 Recovery 296\u003c\/p\u003e \u003cp\u003e19.5 Peak-height and peak-area determination for quantitative analysis 299\u003c\/p\u003e \u003cp\u003e19.6 Integration errors 303\u003c\/p\u003e \u003cp\u003e19.7 The detection wavelength 304\u003c\/p\u003e \u003cp\u003e19.8 Derivatization 306\u003c\/p\u003e \u003cp\u003e19.9 Unexpected peaks: Ghost and system peaks 308\u003c\/p\u003e \u003cp\u003e\u003cb\u003e20 Quality Assurance 311\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e20.1 Is it worth the effort? 311\u003c\/p\u003e \u003cp\u003e20.2 Verification with a second method 312\u003c\/p\u003e \u003cp\u003e20.3 Method validation 312\u003c\/p\u003e \u003cp\u003e20.4 Standard operating procedures 314\u003c\/p\u003e \u003cp\u003e20.5 Measurement uncertainty 315\u003c\/p\u003e \u003cp\u003e20.6 Qualifications, instrument test and system suitability test 317\u003c\/p\u003e \u003cp\u003e20.7 The quest for quality 318\u003c\/p\u003e \u003cp\u003e\u003cb\u003e21 Preparative HPLC 321\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e21.1 Problem 321\u003c\/p\u003e \u003cp\u003e21.2 Preparative HPLC in practice 322\u003c\/p\u003e \u003cp\u003e21.3 Overloading effects 325\u003c\/p\u003e \u003cp\u003e21.4 Fraction collection 328\u003c\/p\u003e \u003cp\u003e21.5 Recycling 330\u003c\/p\u003e \u003cp\u003e21.6 Displacement chromatography 331\u003c\/p\u003e \u003cp\u003e\u003cb\u003e22 Separation of Enantiomers 333\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e22.1 Introduction 333\u003c\/p\u003e \u003cp\u003e22.2 Chiral mobile phases 335\u003c\/p\u003e \u003cp\u003e22.3 Chiral liquid stationary phases 336\u003c\/p\u003e \u003cp\u003e22.4 Chiral solid stationary phases 337\u003c\/p\u003e \u003cp\u003e22.5 Indirect separation of enantiomers 345\u003c\/p\u003e \u003cp\u003e\u003cb\u003e23 Special Possibilities 349\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e23.1 Micro, capillary and chip HPLC 349\u003c\/p\u003e \u003cp\u003e23.2 High-speed and super-speed HPLC 352\u003c\/p\u003e \u003cp\u003e23.3 Fast separations at 1000 bar: UHPLC 353\u003c\/p\u003e \u003cp\u003e23.4 HPLC with supercritical mobile phases 355\u003c\/p\u003e \u003cp\u003e23.5 HPLC with superheated water 359\u003c\/p\u003e \u003cp\u003e23.6 Electrochromatography 361\u003c\/p\u003e \u003cp\u003e\u003cb\u003e24 Appendix 1: Applied HPLC Theory 363\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e25 Appendix 2: How to Perform the Instrument Test 373\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e25.1 Introduction 373\u003c\/p\u003e \u003cp\u003e25.2 Test sequence 373\u003c\/p\u003e \u003cp\u003e25.3 Preparations 374\u003c\/p\u003e \u003cp\u003e25.4 Pump test. 377\u003c\/p\u003e \u003cp\u003e25.5 UV detector test 379\u003c\/p\u003e \u003cp\u003e25.6 Autosampler test 383\u003c\/p\u003e \u003cp\u003e25.7 Column oven test 383\u003c\/p\u003e \u003cp\u003e25.8 Equations and calculations. 384\u003c\/p\u003e \u003cp\u003e25.9 Documentation 385\u003c\/p\u003e \u003cp\u003e\u003cb\u003e26 Appendix 3: Troubleshooting 387\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e26.1 Pressure problems 387\u003c\/p\u003e \u003cp\u003e26.2 Leak in the pump system 389\u003c\/p\u003e \u003cp\u003e26.3 Deviating retention times 389\u003c\/p\u003e \u003cp\u003e26.4 Injection problems 390\u003c\/p\u003e \u003cp\u003e26.5 Baseline problems 390\u003c\/p\u003e \u003cp\u003e26.6 Peak shape problems 392\u003c\/p\u003e \u003cp\u003e26.7 Problems with light-scattering detectors 393\u003c\/p\u003e \u003cp\u003e26.8 Other causes 394\u003c\/p\u003e \u003cp\u003e26.9 Instrument test 395\u003c\/p\u003e \u003cp\u003e\u003cb\u003e27 Appendix 4: Column Packing 397\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIndex of Separations 401\u003c\/p\u003e \u003cp\u003eSubject Index 403\u003c\/p\u003e  \u003cp\u003e\u003cstrong\u003eVeronika R. Meyer\u003c\/strong\u003e, Department of Biocompatible Materials, Switzerland Swiss Federal Laboratories for Materials Testing and Research, Switzerland.  \u003cb\u003eJump into the HPLC adventure!\u003c\/b\u003e \u003c\/p\u003e\u003cp\u003eThree decades on from publication of the 1st German edition of Veronika Meyer's book on HPLC, this classic text remains one of the few titles available on general HPLC aimed at practitioners.\u003c\/p\u003e \u003cp\u003eNew sections on the following topics have been included in this fifth edition:\u003c\/p\u003e \u003cul\u003e \u003cli\u003eComparison of HPLC with capillary electrophoresis\u003c\/li\u003e \u003cli\u003eHow to obtain peak capacity\u003c\/li\u003e \u003cli\u003evan Deemter curves and other coherences\u003c\/li\u003e \u003cli\u003eHydrophilic interaction chromatography\u003c\/li\u003e \u003cli\u003eMethod transfer\u003c\/li\u003e \u003cli\u003eComprehensive two-dimensional HPLC\u003c\/li\u003e \u003cli\u003eFast separations at 1000 bar\u003c\/li\u003e \u003cli\u003eHPLC with superheated water\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eIn addition, two chapters on the instrument test and troubleshooting in the appendix have been updated and expanded by Bruno E. Lendi, and many details have been improved and numerous references added.\u003c\/p\u003e \u003cp\u003eA completely new chapter is presented on quality assurance covering:\u003c\/p\u003e \u003cul\u003e \u003cli\u003eIs it worth the effort?\u003c\/li\u003e \u003cli\u003eVerification with a second method\u003c\/li\u003e \u003cli\u003eMethod validation\u003c\/li\u003e \u003cli\u003eStandard operating procedures\u003c\/li\u003e \u003cli\u003eMeasurement uncertainty\u003c\/li\u003e \u003cli\u003eQualifications, instrument test, and system suitability test\u003c\/li\u003e \u003cli\u003eThe quest for quality\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003cb\u003eReviews of earlier editions\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\"That this text is written by an expert in both the practice and teaching of HPLC is evident from the first paragraph....not only an enjoyable, fascinating and easy read, but a truly excellent text that has and will serve many teachers, students and practitioners very well.\" —\u003ci\u003eThe Analyst\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e“…provides essential information on HPLC for LC practitioners in academia, industry, government, and research laboratories…a valuable introduction.\" - \u003ci\u003eAmerican Journal of Therapeutics\u003c\/i\u003e\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47989832450277,"sku":"NP9780470682180","price":202.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9780470682180.jpg?v=1761785615","url":"https:\/\/k12savings.com\/products\/practical-high-performance-liquid-chromatography-isbn-9780470682180","provider":"K12savings","version":"1.0","type":"link"}