{"product_id":"practical-high-performance-liquid-chromatography-isbn-9780470682173","title":"Practical High-Performance Liquid Chromatography","description":"\u003cb\u003eJump into the HPLC adventure!\u003c\/b\u003e  \u003cp\u003eThree decades on from publication of the 1\u003csup\u003est\u003c\/sup\u003e German edition of Veronika Meyer's book on HPLC, this classic text remains one of the few titles available on general HPLC aimed at practitioners.\u003c\/p\u003e \u003cp\u003eNew sections on the following topics have been included in this fifth edition:\u003c\/p\u003e \u003cul type=\"disc\"\u003e \u003cli\u003eComparison of HPLC with capillary electrophoresis\u003c\/li\u003e \u003cli\u003eHow to obtain peak capacity\u003c\/li\u003e \u003cli\u003evan Deemter curves and other coherences\u003c\/li\u003e \u003cli\u003eHydrophilic interaction chromatography\u003c\/li\u003e \u003cli\u003eMethod transfer\u003c\/li\u003e \u003cli\u003eComprehensive two-dimensional HPLC\u003c\/li\u003e \u003cli\u003eFast separations at 1000 bar\u003c\/li\u003e \u003cli\u003eHPLC with superheated water\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eIn addition, two chapters on the instrument test and troubleshooting in the appendix have been updated and expanded by Bruno E. Lendi, and many details have been improved and numerous references added.\u003c\/p\u003e \u003cp\u003eA completely new chapter is presented on quality assurance covering:\u003c\/p\u003e \u003cul type=\"disc\"\u003e \u003cli\u003eIs it worth the effort?\u003c\/li\u003e \u003cli\u003eVerification with a second method\u003c\/li\u003e \u003cli\u003eMethod validation\u003c\/li\u003e \u003cli\u003eStandard operating procedures\u003c\/li\u003e \u003cli\u003eMeasurement uncertainty\u003c\/li\u003e \u003cli\u003eQualifications, instrument test, and system suitability test\u003c\/li\u003e \u003cli\u003eThe quest for quality\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003cb\u003eReviews of earlier editions\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\"That this text is written by an expert in both the practice and teaching of HPLC is evident from the first paragraph....not only an enjoyable, fascinating and easy read, but a truly excellent text that has and will serve many teachers, students and practitioners very well.\" —\u003ci\u003eThe Analyst\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e“…provides essential information on HPLC for LC practitioners in academia, industry, government, and research laboratories…a valuable introduction.\" - \u003ci\u003eAmerican Journal of Therapeutics\u003c\/i\u003e\u003c\/p\u003e  Preface to the Fifth Edition.  \u003cp\u003eImportant and Useful Equations for HPLC.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Introduction.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 HPLC: A powerful separation method.\u003c\/p\u003e \u003cp\u003e1.2 A first HPLC experiment.\u003c\/p\u003e \u003cp\u003e1.3 Liquid chromatographic separation modes.\u003c\/p\u003e \u003cp\u003e1.4 The HPLC instrument.\u003c\/p\u003e \u003cp\u003e1.5 Safety in the HPLC laboratory.\u003c\/p\u003e \u003cp\u003e1.6 Comparison between high-performance liquid chromatography and gas chromatography.\u003c\/p\u003e \u003cp\u003e1.7 Comparison between high-performance liquid chromatography and capillary electrophoresis.\u003c\/p\u003e \u003cp\u003e1.8 Units for pressure, length and viscosity.\u003c\/p\u003e \u003cp\u003e1.9 Scientific journals.\u003c\/p\u003e \u003cp\u003e1.10 Recommended books.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Theoretical Principles.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 The chromatographic process.\u003c\/p\u003e \u003cp\u003e2.2 Band broadening.\u003c\/p\u003e \u003cp\u003e2.3 The chromatogram and its purport.\u003c\/p\u003e \u003cp\u003e2.4 Graphical representation of peak pairs with different degree of resolution.\u003c\/p\u003e \u003cp\u003e2.5 Factors affecting resolution.\u003c\/p\u003e \u003cp\u003e2.6 Extra-column volumes (dead volumes).\u003c\/p\u003e \u003cp\u003e2.7 Tailing.\u003c\/p\u003e \u003cp\u003e2.8 Peak capacity and statistical resolution probability.\u003c\/p\u003e \u003cp\u003e2.9 Effects of temperature in HPLC.\u003c\/p\u003e \u003cp\u003e2.10 The limits of HPLC.\u003c\/p\u003e \u003cp\u003e2.11 How to obtain peak capacity.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Pumps.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 General requirements.\u003c\/p\u003e \u003cp\u003e3.2 The short-stroke piston pump.\u003c\/p\u003e \u003cp\u003e3.3 Maintenance and repair.\u003c\/p\u003e \u003cp\u003e3.4 Other pump designs.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Preparation of Equipment up to Sample Injection.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Selection of the mobile phase.\u003c\/p\u003e \u003cp\u003e4.2 Preparation of the mobile phase.\u003c\/p\u003e \u003cp\u003e4.3 Gradient systems.\u003c\/p\u003e \u003cp\u003e4.4 Capillary tubing.\u003c\/p\u003e \u003cp\u003e4.5 Fittings.\u003c\/p\u003e \u003cp\u003e4.6 Sample injectors.\u003c\/p\u003e \u003cp\u003e4.7 Sample solution and sample volume.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Solvent Properties.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Table of organic solvents.\u003c\/p\u003e \u003cp\u003e5.2 Solvent selectivity.\u003c\/p\u003e \u003cp\u003e5.3 Miscibility.\u003c\/p\u003e \u003cp\u003e5.4 Buffers.\u003c\/p\u003e \u003cp\u003e5.5 Shelf life of mobile phases.\u003c\/p\u003e \u003cp\u003e5.6 The mixing cross.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Detectors.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 General.\u003c\/p\u003e \u003cp\u003e6.2 UV detectors.\u003c\/p\u003e \u003cp\u003e6.3 Refractive index detectors.\u003c\/p\u003e \u003cp\u003e6.4 Fluorescence detectors.\u003c\/p\u003e \u003cp\u003e6.5 Electrochemical (amperometric) detectors.\u003c\/p\u003e \u003cp\u003e6.6 Light-scattering detectors.\u003c\/p\u003e \u003cp\u003e6.7 Other detectors.\u003c\/p\u003e \u003cp\u003e6.8 Multiple detection.\u003c\/p\u003e \u003cp\u003e6.9 Indirect detection.\u003c\/p\u003e \u003cp\u003e6.10 Coupling with spectroscopy.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Columns and Stationary Phases.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Columns for HPLC.\u003c\/p\u003e \u003cp\u003e7.2 Precolumns.\u003c\/p\u003e \u003cp\u003e7.3 General properties of stationary phases.\u003c\/p\u003e \u003cp\u003e7.4 Silica.\u003c\/p\u003e \u003cp\u003e7.5 Chemically modified silica.\u003c\/p\u003e \u003cp\u003e7.6 Styrene-divinylbenzene.\u003c\/p\u003e \u003cp\u003e7.7 Some other stationary phases.\u003c\/p\u003e \u003cp\u003e7.8 Column care and regeneration.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 HPLC Column Tests.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 Simple tests for HPLC columns.\u003c\/p\u003e \u003cp\u003e8.2 Determination of particle size.\u003c\/p\u003e \u003cp\u003e8.3 Determination of breakthrough time.\u003c\/p\u003e \u003cp\u003e8.4 The test mixture.\u003c\/p\u003e \u003cp\u003e8.5 Dimensionless parameters for HPLC column characterization.\u003c\/p\u003e \u003cp\u003e8.6 The van Deemter equation from reduced parameters and its use in column diagnosis.\u003c\/p\u003e \u003cp\u003e8.7 van Deemter curves and other coherences.\u003c\/p\u003e \u003cp\u003e8.8 Diffusion coefficients.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Adsorption Chromatography: Normal-Phase Chromatography.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 What is adsorption?.\u003c\/p\u003e \u003cp\u003e9.2 The eluotropic series.\u003c\/p\u003e \u003cp\u003e9.3 Selectivity properties of the mobile phase.\u003c\/p\u003e \u003cp\u003e9.4 Choice and optimization of the mobile phase.\u003c\/p\u003e \u003cp\u003e9.5 Applications.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Reversed-Phase Chromatography.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e10.1 Principle.\u003c\/p\u003e \u003cp\u003e10.2 Mobile phases in reversed-phase chromatography.\u003c\/p\u003e \u003cp\u003e10.3 Solvent selectivity and strength.\u003c\/p\u003e \u003cp\u003e10.4 Stationary phases.\u003c\/p\u003e \u003cp\u003e10.5 Method development in reversed-phase chromatography.\u003c\/p\u003e \u003cp\u003e10.6 Applications.\u003c\/p\u003e \u003cp\u003e10.7 Hydrophobic interaction chromatography.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Chromatography with Chemically Bonded Phases.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e11.1 Introduction.\u003c\/p\u003e \u003cp\u003e11.2 Properties of some stationary phases.\u003c\/p\u003e \u003cp\u003e11.3 Hydrophilic interaction chromatography.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Ion-Exchange Chromatography.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction.\u003c\/p\u003e \u003cp\u003e12.2 Principle.\u003c\/p\u003e \u003cp\u003e12.3 Properties of ion exchangers.\u003c\/p\u003e \u003cp\u003e12.4 Influence of the mobile phase.\u003c\/p\u003e \u003cp\u003e12.5 Special possibilities of ion exchange.\u003c\/p\u003e \u003cp\u003e12.6 Practical hints.\u003c\/p\u003e \u003cp\u003e12.7 Applications.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Ion-Pair Chromatography.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e13.1 Introduction.\u003c\/p\u003e \u003cp\u003e13.2 Ion-pair chromatography in practice.\u003c\/p\u003e \u003cp\u003e13.3 Applications.\u003c\/p\u003e \u003cp\u003e13.4 Appendix: UV detection using ion-pair reagents.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 Ion Chromatography.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e14.1 Principle.\u003c\/p\u003e \u003cp\u003e14.2 Suppression techniques.\u003c\/p\u003e \u003cp\u003e14.3 Phase systems.\u003c\/p\u003e \u003cp\u003e14.4 Applications.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 Size-Exclusion Chromatography.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e15.1 Principle.\u003c\/p\u003e \u003cp\u003e15.2 The calibration chromatogram.\u003c\/p\u003e \u003cp\u003e15.3 Molecular mass determination by means of size-exclusion chromatography.\u003c\/p\u003e \u003cp\u003e15.4 Coupled size-exclusion columns.\u003c\/p\u003e \u003cp\u003e15.5 Phase systems.\u003c\/p\u003e \u003cp\u003e15.6 Applications.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e16 Affinity Chromatography.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e16.1 Principle.\u003c\/p\u003e \u003cp\u003e16.2 Affinity chromatography as a special case of HPLC.\u003c\/p\u003e \u003cp\u003e16.3 Applications.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e17 Choice of Method.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e17.1 The various possibilities.\u003c\/p\u003e \u003cp\u003e17.2 Method transfer.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e18 Solving the Elution Problem.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e18.1 The elution problem.\u003c\/p\u003e \u003cp\u003e18.2 Solvent gradients.\u003c\/p\u003e \u003cp\u003e18.3 Column switching.\u003c\/p\u003e \u003cp\u003e18.4 Comprehensive two-dimensional HPLC.\u003c\/p\u003e \u003cp\u003e18.5 Optimization of an isocratic chromatogram using four solvents.\u003c\/p\u003e \u003cp\u003e18.6 Optimization of the other parameters.\u003c\/p\u003e \u003cp\u003e18.7 Mixed stationary phases.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e19 Analytical HPLC.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e19.1 Qualitative analysis.\u003c\/p\u003e \u003cp\u003e19.2 Trace analysis.\u003c\/p\u003e \u003cp\u003e19.3 Quantitative analysis.\u003c\/p\u003e \u003cp\u003e19.4 Recovery.\u003c\/p\u003e \u003cp\u003e19.5 Peak-height and peak-area determination for quantitative analysis.\u003c\/p\u003e \u003cp\u003e19.6 Integration errors.\u003c\/p\u003e \u003cp\u003e19.7 The detection wavelength.\u003c\/p\u003e \u003cp\u003e19.8 Derivatization.\u003c\/p\u003e \u003cp\u003e19.9 Unexpected peaks: Ghost and system peaks.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e20 Quality Assurance.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e20.1 Is it worth the effort?.\u003c\/p\u003e \u003cp\u003e20.2 Verification with a second method.\u003c\/p\u003e \u003cp\u003e20.3 Method validation.\u003c\/p\u003e \u003cp\u003e20.4 Standard operating procedures.\u003c\/p\u003e \u003cp\u003e20.5 Measurement uncertainty.\u003c\/p\u003e \u003cp\u003e20.6 Qualifications, instrument test and system suitability test.\u003c\/p\u003e \u003cp\u003e20.7 The quest for quality.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e21 Preparative HPLC.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e21.1 Problem.\u003c\/p\u003e \u003cp\u003e21.2 Preparative HPLC in practice.\u003c\/p\u003e \u003cp\u003e21.3 Overloading effects.\u003c\/p\u003e \u003cp\u003e21.4 Fraction collection.\u003c\/p\u003e \u003cp\u003e21.5 Recycling.\u003c\/p\u003e \u003cp\u003e21.6 Displacement chromatography.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e22 Separation of Enantiomers.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e22.1 Introduction.\u003c\/p\u003e \u003cp\u003e22.2 Chiral mobile phases.\u003c\/p\u003e \u003cp\u003e22.3 Chiral liquid stationary phases.\u003c\/p\u003e \u003cp\u003e22.4 Chiral solid stationary phases.\u003c\/p\u003e \u003cp\u003e22.5 Indirect separation of enantiomers.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e23 Special Possibilities.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e23.1 Micro, capillary and chip HPLC.\u003c\/p\u003e \u003cp\u003e23.2 High-speed and super-speed HPLC.\u003c\/p\u003e \u003cp\u003e23.3 Fast separations at 1000 bar: UHPLC.\u003c\/p\u003e \u003cp\u003e23.4 HPLC with supercritical mobile phases.\u003c\/p\u003e \u003cp\u003e23.5 HPLC with superheated water.\u003c\/p\u003e \u003cp\u003e23.6 Electrochromatography.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e24 Appendix 1: Applied HPLC Theory.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e25 Appendix 2: How to Perform the Instrument Test.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e25.1 Introduction.\u003c\/p\u003e \u003cp\u003e25.2 Test sequence.\u003c\/p\u003e \u003cp\u003e25.3 Preparations.\u003c\/p\u003e \u003cp\u003e25.4 Pump test.\u003c\/p\u003e \u003cp\u003e25.5 UV detector test.\u003c\/p\u003e \u003cp\u003e25.6 Autosampler test.\u003c\/p\u003e \u003cp\u003e25.7 Column oven test.\u003c\/p\u003e \u003cp\u003e25.8 Equations and calculations.\u003c\/p\u003e \u003cp\u003e25.9 Documentation.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e26 Appendix 3: Troubleshooting.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e26.1 Pressure problems.\u003c\/p\u003e \u003cp\u003e26.2 Leak in the pump system.\u003c\/p\u003e \u003cp\u003e26.3 Deviating retention times.\u003c\/p\u003e \u003cp\u003e26.4 Injection problems.\u003c\/p\u003e \u003cp\u003e26.5 Baseline problems.\u003c\/p\u003e \u003cp\u003e26.6 Peak shape problems.\u003c\/p\u003e \u003cp\u003e26.7 Problems with light-scattering detectors.\u003c\/p\u003e \u003cp\u003e26.8 Other causes.\u003c\/p\u003e \u003cp\u003e26.9 Instrument test.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e27 Appendix 4: Column Packing.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eIndex of Separations.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSubject Index.\u003c\/b\u003e\u003c\/p\u003e  \"This book is very much ‘‘an aid to this end'' and should be number one on every young chromatographer's book list. The publishers too have played their part in producing a book that is pleasing on the eye and a pleasure to read.\" (Chromatographia, 1 December 2010)\u003cbr\u003e  \u003cp\u003e\u003cstrong\u003eVeronika R. Meyer\u003c\/strong\u003e, Department of Biocompatible Materials, Switzerland Swiss Federal Laboratories for Materials Testing and Research, Switzerland.   \u003cb\u003eJump into the HPLC adventure!\u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003eThree decades on from publication of the 1\u003csup\u003est\u003c\/sup\u003e German edition of Veronika Meyer's book on HPLC, this classic text remains one of the few titles available on general HPLC aimed at practitioners.\u003c\/p\u003e \u003cp\u003eNew sections on the following topics have been included in this fifth edition:\u003c\/p\u003e \u003cul type=\"disc\"\u003e \u003cli\u003eComparison of HPLC with capillary electrophoresis\u003c\/li\u003e \u003cli\u003eHow to obtain peak capacity\u003c\/li\u003e \u003cli\u003evan Deemter curves and other coherences\u003c\/li\u003e \u003cli\u003eHydrophilic interaction chromatography\u003c\/li\u003e \u003cli\u003eMethod transfer\u003c\/li\u003e \u003cli\u003eComprehensive two-dimensional HPLC\u003c\/li\u003e \u003cli\u003eFast separations at 1000 bar\u003c\/li\u003e \u003cli\u003eHPLC with superheated water\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eIn addition, two chapters on the instrument test and troubleshooting in the appendix have been updated and expanded by Bruno E. Lendi, and many details have been improved and numerous references added.\u003c\/p\u003e \u003cp\u003eA completely new chapter is presented on quality assurance covering:\u003c\/p\u003e \u003cul type=\"disc\"\u003e \u003cli\u003eIs it worth the effort?\u003c\/li\u003e \u003cli\u003eVerification with a second method\u003c\/li\u003e \u003cli\u003eMethod validation\u003c\/li\u003e \u003cli\u003eStandard operating procedures\u003c\/li\u003e \u003cli\u003eMeasurement uncertainty\u003c\/li\u003e \u003cli\u003eQualifications, instrument test, and system suitability test\u003c\/li\u003e \u003cli\u003eThe quest for quality\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003cb\u003eReviews of earlier editions\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\"That this text is written by an expert in both the practice and teaching of HPLC is evident from the first paragraph....not only an enjoyable, fascinating and easy read, but a truly excellent text that has and will serve many teachers, students and practitioners very well.\" —\u003ci\u003eThe Analyst\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e“…provides essential information on HPLC for LC practitioners in academia, industry, government, and research laboratories…a valuable introduction.\" - \u003ci\u003eAmerican Journal of Therapeutics\u003c\/i\u003e\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47989832679653,"sku":"NP9780470682173","price":82.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9780470682173.jpg?v=1761785616","url":"https:\/\/k12savings.com\/products\/practical-high-performance-liquid-chromatography-isbn-9780470682173","provider":"K12savings","version":"1.0","type":"link"}