Molecular Biology Problem Solver

Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems.

Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as:

• Have you selected the most appropriate research strategy?
• Have you identified the issues critical to your successful application of a technique?
• Are you familiar with the limitations of a given technique?
• When should common procedural rules of thumb not be applied?
• What strategies could you apply to resolve a problem?

A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include:

• Working safely with biological samples and radioactive materials
• DNA and RNA purification
• PCR
• Protein and nucleid acid hybridization
• Prokaryotic and eukaryotic expression systems
• Properly using and maintaining laboratory equipment

Der "Molecular Biology Problem Solver" ist ein einzigartiger Laborleitfaden für Molekularbiologen. Hier lernen Sie, die kritischen Elemente von Techniken, Reagenzien und Verfahren zu erkennen und Ihre Fähigkeiten zur Fehleranalyse und -behebung bei der Durchführung von Experimenten deutlich zu verbessern. Der hier behandelte Stoff dient als Grundlage für die Entwicklung fundierter analytischer Laborkenntnisse. Damit bietet dieses Buch eine solide Basis für ein besseres Verständnis möglicher Fehlerursachen im Laboralltag. Es soll der Forschungsgemeinschaft - vom Wissenschaftler bis hin zum Techniker - dabei helfen, unabhängig verschiedene Situationen und Problemlösungen auszuarbeiten und künftig Probleme zu vermeiden. Schwerpunktmäßig werden u.a. folgende Themen behandelt: Gefährliche Stoffe, Benutzung und Wartung der Laboreinrichtung, Bakteriologie, DNA und RNA und Elektrophorese. Der "Molecular Biology Problem Solver" liefert eine ausgewogene Darstellung von Theorie und Praxis und enthält wichtige Informationen, die anderenorts schwer zugänglich sind und die auch in den sehr umfangreichen und detaillierten Current Protocols Series nicht aufgeführt sind. Damit wird dieses Buch zu einer unverzichtbaren Informationsquelle und zu einem absoluten Muss für jeden Molekularbiologen. Preface.

Contributors.

Preparing for Success in the Laboratory (P. Franciskovich).

Getting What You Need from a Supplier (T. Tyre & G. Krueger).

The Preparation of Buffers and Other Solutions: A Chemist's Perspective (E. Pfannkoch).

How to Properly Use and Maintain Laboratory Equipment (T. Troutman, et al.).

Working Safely with Biological Samples (C. Haidaris & E. Brownlow).

Working Safely with Radioactive Materials (W. Volny).

DNA Purification (S. Herzer).

RNA Purification (L. Martin, et al.).

Restriction Endonucleases (D. Robinson, et al.).

Nucleotides, Oligonucleotides, and Polynucleotides (A. Gerstein).

PCR (K. Aoyagi).

Electrophoresis (M. Booz).

Western Blotting (P. Riis).

Nucleic Acid Hybridization (S. Herzer & D. Englert).

E. coli Expression Systems (P. Bell).

Eukaryotic Expression (J. Trill, et al.).

Index. "It will strengthen your ability to prevent, identify and solve problems in the lab..." (Medical Laboratory World, October 2001) ALAN GERSTEIN and his collaborators have been guiding researchers of all levels of expertise through the troubleshooting process over the course of three decades, assuring that Molecular Biology Problem Solver will prove an excellent resource for all life scientists. Most research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems. Molecular Biology Problem Solver: A Laboratory Guide asks the reader to consider crucial questions, such as:
* Have you selected the most appropriate research strategy?
* Have you identified the issues critical to your successful application of a technique?
* Are you familiar with the limitations of a given technique?
* When should common procedural rules of thumb not be applied?
* What strategies could you apply to resolve a problem?

A unique question-based format reviews common assumptions and laboratory practices, with the aim of offering a firm understanding of how techniques and procedures work, as well as how to avoid problems. Some major issues explored by the book's expert contributors include:
* Working safely with biological samples and radioactive materials
* DNA and RNA purification
* PCR
* Protein and nucleid acid hybridization
* Prokaryotic and eukaryotic expression systems
* Properly using and maintaining laboratory equipment