{"product_id":"molecular-biology-in-cellular-pathology-isbn-9780470844755","title":"Molecular Biology in Cellular Pathology","description":"The latest edition of this highly successful text, covers the major advances in the methods used in cellular and molecular pathology. In recent years, knowledge of the molecular organization of the cell has led to the development of powerful new techniques that bring greater accuracy and objectives to the diagnosis, prognosis and management of many diseases and to the study of pathological states. This book describes the latest molecular techniques available for the analysis of diseases. In particular it includes new techniques using fluorescent dyes, DNA microarrays, protein chemistry, and mass spectrometry. It also incorporates information from the Human Genome Project, and the new disciplines of genomics and proteomics, where relevant to pathology. Color plates are a new feature of this edition, illustrating the advances in fluorescence labeling of cells.\"Molecular Biology in Cellular Pathology\" - Jetzt erscheint dieser erfolgreiche Klassiker in der 2. aktualisierten Auflage.\u003cbr\u003e \u003cbr\u003e Der Band beschreibt die wichtigsten Fortschritte in der Zell- und Molekularbiologie.\u003cbr\u003e \u003cbr\u003e In den letzten Jahren haben neue Erkenntnisse über den molekularen Aufbau der Zelle zur Entwicklung neuer, leistungsstarker Analysetechniken geführt, die eine größere Genauigkeit bei der Diagnose, Prognose und Behandlung von Krankheiten sowie bei der Untersuchung pathologischer Stadien ermöglichen.\u003cbr\u003e \u003cbr\u003e Insbesondere werden neue Verfahren diskutiert, die fluoreszierende Farbstoffe, DNA Microarrays, Proteinchemie und Massenspektrometrie einsetzen.\u003cbr\u003e \u003cbr\u003e Darüber hinaus enthält das Buch Informationen zum Human-Genom-Projekt und zu den neuen Disziplinen der Genomik und Proteomik, soweit diese für die Pathologie relevant sind.\u003cbr\u003e \u003cbr\u003e Neu an dieser 2. Auflage sind die Farbtafeln, die die enormen Fortschritte in der Fluoreszenz-Markierung von Zellmaterial anschaulich belegen. \u003cp\u003ePreface xiii\u003c\/p\u003e \u003cp\u003ePreface to Molecular Biology in Histopathology xv\u003c\/p\u003e \u003cp\u003eList of Contributors xvii\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Blotting Techniques: Methodology and Applications 1\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eFiona Watson and C. Simon Herrington\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e1.1 Introduction 1\u003c\/p\u003e \u003cp\u003e1.2 Blotting techniques 1\u003c\/p\u003e \u003cp\u003e1.3 References 15\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 In-situ Hybridisation in Histopathology 19\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eGerald Niedobitek and Hermann Herbst\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2.1 Introduction 19\u003c\/p\u003e \u003cp\u003e2.2 Experimental conditions 20\u003c\/p\u003e \u003cp\u003e2.3 Probes and labels 23\u003c\/p\u003e \u003cp\u003e2.4 Controls and pitfalls 27\u003c\/p\u003e \u003cp\u003e2.5 Double-labelling 29\u003c\/p\u003e \u003cp\u003e2.6 Increasing the sensitivity of ISH 31\u003c\/p\u003e \u003cp\u003e2.7 What we do in our laboratories 33\u003c\/p\u003e \u003cp\u003e2.8 Applications of ISH: examples 35\u003c\/p\u003e \u003cp\u003e2.9 Perspective 39\u003c\/p\u003e \u003cp\u003e2.10 References 40\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 DNA Flow Cytometry 49\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eM.G. Ormerod\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introduction 49\u003c\/p\u003e \u003cp\u003e3.2 Definitions and terms 49\u003c\/p\u003e \u003cp\u003e3.3 Dye used for DNA analysis 50\u003c\/p\u003e \u003cp\u003e3.4 Sample preparation for DNA analysis 52\u003c\/p\u003e \u003cp\u003e3.5 Analysis of the DNA histogram 53\u003c\/p\u003e \u003cp\u003e3.6 Quality control 53\u003c\/p\u003e \u003cp\u003e3.7 Computer analysis of the DNA histogram 55\u003c\/p\u003e \u003cp\u003e3.8 Multiparametric measurement 57\u003c\/p\u003e \u003cp\u003e3.9 Acknowledgements 59\u003c\/p\u003e \u003cp\u003e3.10 References 59\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Interphase Cytogenetics 61\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eSara A. Dyer and Jonathan J. Waters\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e4.1 Introduction 61\u003c\/p\u003e \u003cp\u003e4.2 Interphase cytogenetics 62\u003c\/p\u003e \u003cp\u003e4.3 Applications 67\u003c\/p\u003e \u003cp\u003e4.4 Conclusion 76\u003c\/p\u003e \u003cp\u003e4.5 References 77\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Oncogenes 79\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eFiona Macdonald\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction 79\u003c\/p\u003e \u003cp\u003e5.2 Identification of the oncogenes 79\u003c\/p\u003e \u003cp\u003e5.3 Functions of the proto-oncogenes 80\u003c\/p\u003e \u003cp\u003e5.4 Mechanism of oncogene activation 89\u003c\/p\u003e \u003cp\u003e5.5 Oncogenes in colorectal cancer 91\u003c\/p\u003e \u003cp\u003e5.6 Oncogenes in breast cancer 94\u003c\/p\u003e \u003cp\u003e5.7 Oncogenes in lung cancer 95\u003c\/p\u003e \u003cp\u003e5.8 Oncogenes in haematological malignancies 96\u003c\/p\u003e \u003cp\u003e5.9 Other cancers 99\u003c\/p\u003e \u003cp\u003e5.10 Conclusion 100\u003c\/p\u003e \u003cp\u003e5.11 References 100\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Molecular and Immunological Aspects of Cell Proliferation 105\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eKarl Baumforth and John Crocker\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6.1 The cell cycle and its importance in clinical pathology 105\u003c\/p\u003e \u003cp\u003e6.2 Molecular control of the cell cycle 108\u003c\/p\u003e \u003cp\u003e6.3 Cell cycle control 111\u003c\/p\u003e \u003cp\u003e6.4 The cell cycle and cancer 112\u003c\/p\u003e \u003cp\u003e6.5 Immunocytochemical markers of proliferating cells 115\u003c\/p\u003e \u003cp\u003e6.6 References 133\u003c\/p\u003e \u003cp\u003e6.7 Further Reading 135\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Interphase Nucleolar Organiser Regions in Tumour Pathology 137\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eMassimo Derenzini, Davide Treré, Marie-Françoise O’Donohue and Dominique Ploton\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction 137\u003c\/p\u003e \u003cp\u003e7.2 The AgNORs 138\u003c\/p\u003e \u003cp\u003e7.3 NOR silver-staining 142\u003c\/p\u003e \u003cp\u003e7.4 Quantitative AgNOR analysis 145\u003c\/p\u003e \u003cp\u003e7.5 AgNORs as a parameter of the level of cell proliferation 146\u003c\/p\u003e \u003cp\u003e7.6 Application of the AgNOR technique to tumour pathology 147\u003c\/p\u003e \u003cp\u003e7.7 What future for AgNORs in tumour pathology? 151\u003c\/p\u003e \u003cp\u003e7.8 References 152\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Apoptosis and Cell Senescence 153\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eLee B. Jordan and David J. Harrison\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction 153\u003c\/p\u003e \u003cp\u003e8.2 Apoptosis 153\u003c\/p\u003e \u003cp\u003e8.3 Cell senescence 174\u003c\/p\u003e \u003cp\u003e8.4 Summary 178\u003c\/p\u003e \u003cp\u003e8.5 References 179\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 The Polymerase Chain Reaction 193\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eTimothy Diss\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction 193\u003c\/p\u003e \u003cp\u003e9.2 Principles 194\u003c\/p\u003e \u003cp\u003e9.3 Analysis of products 197\u003c\/p\u003e \u003cp\u003e9.4 Rt-pcr 199\u003c\/p\u003e \u003cp\u003e9.5 Quantitative PCR 200\u003c\/p\u003e \u003cp\u003e9.6 DNA and RNA extraction 200\u003c\/p\u003e \u003cp\u003e9.7 Correlation of the PCR with morphology 201\u003c\/p\u003e \u003cp\u003e9.8 Problems 202\u003c\/p\u003e \u003cp\u003e9.9 Applications 202\u003c\/p\u003e \u003cp\u003e9.10 Diagnostic applications 203\u003c\/p\u003e \u003cp\u003e9.11 Infectious diseases 209\u003c\/p\u003e \u003cp\u003e9.12 Identity 209\u003c\/p\u003e \u003cp\u003e9.13 The future 210\u003c\/p\u003e \u003cp\u003e9.14 References 210\u003c\/p\u003e \u003cp\u003e9.15 Online information 212\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Laser Capture Microdissection: Techniques and Applications in the Molecular Analysis of the Cancer Cell 213\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eAmanda Dutton, Victor Lopes and Paul G. Murray\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e10.1 Introduction 213\u003c\/p\u003e \u003cp\u003e10.2 The principle of LCM 214\u003c\/p\u003e \u003cp\u003e10.3 Technical considerations 216\u003c\/p\u003e \u003cp\u003e10.4 Advantages and disadvantages of LCM 217\u003c\/p\u003e \u003cp\u003e10.5 Applications of LCM 222\u003c\/p\u003e \u003cp\u003e10.6 Future perspectives 229\u003c\/p\u003e \u003cp\u003e10.7 Acknowledgements 229\u003c\/p\u003e \u003cp\u003e10.8 References 229\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 The In-situ Polymerase Chain Reaction 233\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJohn J. O’Leary, Cara Martin and Orla Sheils\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e11.1 Introduction 233\u003c\/p\u003e \u003cp\u003e11.2 Overview of the methodology 234\u003c\/p\u003e \u003cp\u003e11.3 In-cell PCR technologies 235\u003c\/p\u003e \u003cp\u003e11.4 In-cell amplification of DNA 238\u003c\/p\u003e \u003cp\u003e11.5 Detection of amplicons 242\u003c\/p\u003e \u003cp\u003e11.6 Reaction, tissue and detection controls for use with in-cell DNA PCR assays 243\u003c\/p\u003e \u003cp\u003e11.7 In-cell RNA amplification 244\u003c\/p\u003e \u003cp\u003e11.8 Problems encountered with in-cell PCR amplification 246\u003c\/p\u003e \u003cp\u003e11.9 Amplicon diffusion and back diffusion 247\u003c\/p\u003e \u003cp\u003e11.10 Future work with in-cell PCR-based assays 247\u003c\/p\u003e \u003cp\u003e11.11 References 249\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 TaqMan® Technology and Real-Time Polymerase Chain Reaction 251\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJohn J. O’Leary, Orla Sheils, Cara Martin, and Aoife Crowley\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction 251\u003c\/p\u003e \u003cp\u003e12.2 Probe technologies 252\u003c\/p\u003e \u003cp\u003e12.3 TaqMan® probe and chemistry (first generation) 254\u003c\/p\u003e \u003cp\u003e12.4 Second generation TaqMan® probes 256\u003c\/p\u003e \u003cp\u003e12.5 Hybridisation 258\u003c\/p\u003e \u003cp\u003e12.6 TaqMan® PCR conditions 259\u003c\/p\u003e \u003cp\u003e12.7 Standards for quantitative PCR 260\u003c\/p\u003e \u003cp\u003e12.8 Interpretation of results 261\u003c\/p\u003e \u003cp\u003e12.9 End-point detection 262\u003c\/p\u003e \u003cp\u003e12.10 Real-time detection 263\u003c\/p\u003e \u003cp\u003e12.11 Relative quantitation 263\u003c\/p\u003e \u003cp\u003e12.12 Reference genes 264\u003c\/p\u003e \u003cp\u003e12.13 Specific TaqMan® PCR applications 265\u003c\/p\u003e \u003cp\u003e12.14 References 268\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Gene Expression Analysis Using Microarrays 269\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eSophie E. Wildsmith and Fiona J. Spence\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e13.1 Introduction 269\u003c\/p\u003e \u003cp\u003e13.2 Microarray experiments 269\u003c\/p\u003e \u003cp\u003e13.3 Data analysis 273\u003c\/p\u003e \u003cp\u003e13.4 Recent examples of microarray applications 284\u003c\/p\u003e \u003cp\u003e13.5 Conclusions 284\u003c\/p\u003e \u003cp\u003e13.6 Acknowledgements 284\u003c\/p\u003e \u003cp\u003e13.7 References 284\u003c\/p\u003e \u003cp\u003e13.8 Further Reading 286\u003c\/p\u003e \u003cp\u003e13.9 Useful websites 286\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 Comparative Genomic Hybridisation in Pathology 287\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eMarjan M. Weiss, Mario A.J.A. Hermsen, Antoine Snijders, Horst Buerger, Werner Boecker, Ernst J. Kuipers, Paul J. van Diest andGerritA.Meijer\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e14.1 Introduction 287\u003c\/p\u003e \u003cp\u003e14.2 Technique 289\u003c\/p\u003e \u003cp\u003e14.3 Data analysis 292\u003c\/p\u003e \u003cp\u003e14.4 Applications 293\u003c\/p\u003e \u003cp\u003e14.5 Clinical applications 299\u003c\/p\u003e \u003cp\u003e14.6 Screening for chromosomal abnormalities in fetal and neonatal genomes 299\u003c\/p\u003e \u003cp\u003e14.7 Future perspectives 300\u003c\/p\u003e \u003cp\u003e14.8 Acknowledgements 301\u003c\/p\u003e \u003cp\u003e14.9 References 301\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 DNA Sequencing and the Human Genome Project 307\u003cbr\u003e \u003c\/b\u003e\u003ci\u003ePhilip Bennett\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e15.1 Introduction 307\u003c\/p\u003e \u003cp\u003e15.2 DNA sequencing: the basics 308\u003c\/p\u003e \u003cp\u003e15.3 Applications of DNA sequencing 318\u003c\/p\u003e \u003cp\u003e15.4 The Human Genome Project 320\u003c\/p\u003e \u003cp\u003e15.5 References 327\u003c\/p\u003e \u003cp\u003e15.6 Further Reading 327\u003c\/p\u003e \u003cp\u003e15.7 Useful websites 328\u003c\/p\u003e \u003cp\u003e\u003cb\u003e16 Monoclonal Antibodies: The Generation and Application of ‘Tools of the Trade’ Within Biomedical Science 329\u003cbr\u003e \u003c\/b\u003e\u003ci\u003ePaul N. Nelson, S. Jane Astley and Philip Warren\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e16.1 Introduction 329\u003c\/p\u003e \u003cp\u003e16.2 Antibodies and antigens 331\u003c\/p\u003e \u003cp\u003e16.3 Polyclonal antibodies 332\u003c\/p\u003e \u003cp\u003e16.4 Monoclonal antibody development 333\u003c\/p\u003e \u003cp\u003e16.5 Monoclonal antibody variants 338\u003c\/p\u003e \u003cp\u003e16.6 Monoclonal antibody applications 341\u003c\/p\u003e \u003cp\u003e16.7 Therapy 345\u003c\/p\u003e \u003cp\u003e16.8 Specific applications 346\u003c\/p\u003e \u003cp\u003e16.9 Conclusions 347\u003c\/p\u003e \u003cp\u003e16.10 Acknowledgements 347\u003c\/p\u003e \u003cp\u003e16.11 References 347\u003c\/p\u003e \u003cp\u003e\u003cb\u003e17 Proteomics 351\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eKathryn Lilley, Azam Razzaq and Michael J. Deery\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e17.1 Introduction 351\u003c\/p\u003e \u003cp\u003e17.2 Definitions and applications 352\u003c\/p\u003e \u003cp\u003e17.3 Stages in proteome analysis 352\u003c\/p\u003e \u003cp\u003e17.4 Future directions 368\u003c\/p\u003e \u003cp\u003e17.5 References 368\u003c\/p\u003e \u003cp\u003eIndex 371\u003c\/p\u003e  \u003cstrong\u003eJohn Crocker\u003c\/strong\u003e is the editor of Molecular Biology in Cellular Pathology, published by Wiley. \u003cp\u003e\u003cstrong\u003ePaul G. Murray\u003c\/strong\u003e is the editor of Molecular Biology in Cellular Pathology, published by Wiley.   Molecular biology has had a major impact on the practice of pathology in recent years and this continues with the new approaches being developed in genomics and proteomics. Knowledge of the molecular organization of the cell has led to the development of powerful new techniques which bring greater accuracy and objectivity to the diagnosis, prognosis and management of many diseases, and to the study of pathological states. This book is a completely revised and updated version of the popular Molecular Biology in Histopathology, with added material covering the latest methodologies and now featuring colour plates. It describes the molecular techniques available and provides an overview of how they can be applied.\u003cbr\u003e \u003cbr\u003e It is aimed at histopathologists in training, clinicians and biologists studying for post-graduate qualifications and will also be of interest to biochemists, physiologists, pharmacologists and biotechnologists, in academia, clinical medicine and industry.\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47989647180005,"sku":"NP9780470844755","price":338.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9780470844755.jpg?v=1761784948","url":"https:\/\/k12savings.com\/products\/molecular-biology-in-cellular-pathology-isbn-9780470844755","provider":"K12savings","version":"1.0","type":"link"}