{"product_id":"methods-in-molecular-biology-and-protein-chemistry-isbn-9780470843604","title":"Methods in Molecular Biology and Protein Chemistry","description":"The enormous advances made in molecular biology have allowed scientists to manipulate DNA with relative ease. This means that molecular biology has become a widely-used tool for answering scientific questions that may be quite unrelated to genetics and cell biology.\u003cbr\u003e \u003cbr\u003e This new book focuses on the most important techniques needed by undergraduate and post-graduate students when undertaking research in this area. Written in an accessible style and adopting a 'discovery' approach, to encourage the students to explore and experiment for them selves, this book will be invaluable to novices required and interested in learning these techniques.\u003cbr\u003e * Students will evaluate the effect of genetic engineering through the practical application of wet biochemistry techniques and prepare mutations that can be evaluated by other classes.\u003cbr\u003e * Coverage will include computer tutorials for sequencing and genomics and will possibly include some coverage of molecular modelling too.\u003cbr\u003e * Guidelines on searching the primary literature, oral reports, laboratory notebooks record keeping and report writing will either be included within appendices at the back of the book, or on a supplementary web site linked to the book.Ein praxisorientierter Leitfaden für Studenten im Hauptstudium.\u003cbr\u003e \u003cbr\u003e Vermittelt werden aktuelle Techniken der Molekularbiologie und Biochemie als fester Bestandteil eines Forschungsprojektes.\u003cbr\u003e \u003cbr\u003e Auf diese Weise kann der Lernende die Auswirkung der Gentechnik anhand der praktischen Anwendung von nassanalytischen biochemischen Verfahren erkennen.\u003cbr\u003e \u003cbr\u003e Mit Computer-Tutorials für Sequenzierung, Genomik und Molekülmodellierung.\u003cbr\u003e \u003cbr\u003e Mit Tipps und Hinweisen zur Sichtung der Primärliteratur, zu mündlichen Berichten, zur Protokollierung des Labortagebuchs und zum Verfassen von Berichten.\u003cbr\u003e \u003cbr\u003e Mit einer Liste von amerikanischen und europäischen kommerziellen Anbietern von Reagenzien.\u003cbr\u003e \u003cbr\u003e Verständlich und anschaulich geschrieben.\u003cbr\u003e \u003cbr\u003e Ein unverzichtbares Lehrbuch für fortgeschrittene Studenten und Doktoranden.\u003cbr\u003e \u003cbr\u003e \"Methods in Molecular Biology\" ist jedoch NICHT geeignet für aktive Forscher auf der Suche nach den neuesten hochdifferenzierten Verfahren. Preface\u003cbr\u003e \u003cbr\u003e Introduction and Background\u003cbr\u003e \u003cbr\u003e Part 1 Molecular Biology\u003cbr\u003e \u003cbr\u003e Experiment 1 Instructions for Pipetman Use\u003cbr\u003e \u003cbr\u003e Experiment 2 Preparation of Plasmid Template DNA\u003cbr\u003e \u003cbr\u003e Experiment 3 Estimation of Plasmid DNA Concentration\u003cbr\u003e PCR Amplification of LTB Gene from Plasmid DNA\u003cbr\u003e \u003cbr\u003e Experiment 4 Agarose Gel Eletrophoresis\u003cbr\u003e Recovery of PCR Product\u003cbr\u003e \u003cbr\u003e Experiment 5 Restriction Digest of LTB Insert\u003cbr\u003e Plate Preparation\u003cbr\u003e \u003cbr\u003e Experiment 6 Purification of Digested Insert and Vector by Agarose Gel Eletrophoresis\u003cbr\u003e Recovery of Digested Insert and Vector\u003cbr\u003e \u003cbr\u003e Experiment 7 Determination of DNA Concentrations\u003cbr\u003e Ligation of Insert into Vector\u003cbr\u003e \u003cbr\u003e Experiment 8 Transformation of Host Cells by Construct\u003cbr\u003e \u003cbr\u003e Experiment 9 Colony-pick PCR to Check for Presence of Insert\u003cbr\u003e Streak Plates with Potential Constructs\u003cbr\u003e Inoculate Cultures\/Run PCR\u003cbr\u003e \u003cbr\u003e Experiment 10 Agarose Gel Electrophoresis to Verify Presence of Insert\u003cbr\u003e Purification of the Plasmid Construct using a Plasmid Miniprep Kit\u003cbr\u003e \u003cbr\u003e Experiment 11 DNA Concentration Determination\u003cbr\u003e Ethanol Precipitation of Plasmid DNA\u003cbr\u003e Sequencing Reaction\u003cbr\u003e \u003cbr\u003e Experiment 12 Purification of Extension Reaction Products\u003cbr\u003e Sequencing Gel Demonstration\u003cbr\u003e \u003cbr\u003e Experiment 13 Analysis of Sequencing Data\u003cbr\u003e Verification of Insert Sequence\u003cbr\u003e \u003cbr\u003e Experiment 14 Gene Expression\u003cbr\u003e Transformation into Expression Host\u003cbr\u003e \u003cbr\u003e Experiment 15 Induction of LTB Gene Expression\u003cbr\u003e \u003cbr\u003e Part 2 Protein Chemistry\u003cbr\u003e \u003cbr\u003e Experiment 16 SDS-PAGE of Induction Time Course\u003cbr\u003e Transfer of Protein to Membrane for Western Blot\u003cbr\u003e \u003cbr\u003e Experiment 17 Visualization of Western Blot\u003cbr\u003e \u003cbr\u003e Experiment 18 Large Scale Culture Preparation\u003cbr\u003e \u003cbr\u003e Experiment 19 Isolation of LTB by Affinity Chromatography\u003cbr\u003e \u003cbr\u003e Experiment 20 SDS-PAGE of Column Fractions\u003cbr\u003e Immunoblot to verify Presence of LTB\u003cbr\u003e \u003cbr\u003e Experiment 21 Protein Concentration\u003cbr\u003e Protein Crystallization\u003cbr\u003e \u003cbr\u003e Experiment 22 Setting up an ELISA Microtiter Plate\u003cbr\u003e \u003cbr\u003e Experiment 23 ELISA of a Panel of Ganglioside Ligands\u003cbr\u003e \u003cbr\u003e Experiment 24 Protein Structure Determination: X-ray Diffraction Techniques\u003cbr\u003e \u003cbr\u003e Experiment 25 Characterization of a Protein Crystal\u003cbr\u003e \u003cbr\u003e Experiment 26 Primer Design  \u003cp\u003eBrenda Spangler has over 30 years experience in the field of protein chemistry and protein toxins, specializing in ligand-receptior molecular recognition.  Methods in Molecular Biology is a discovery-based guide that will enable students to master current molecular biology and biochemistry techniques as an integrated part of a research project. Written in an accessible style, this book adopts a practical hands-on approach to encourage the reader to explore and experiment independently. The book is designed to support a one semester laboratory course in which students participate in an ongoing research project, give a research presentation on a topic related to the project, and write a research article suitable for publication.\u003cbr\u003e \u003cbr\u003e Methods in Molecular Biology features:\u003cbr\u003e * a carefully structured sequence of experiments that enables students to develop key laboratory skills whilst contributing towards a live, research project.\u003cbr\u003e \u003cbr\u003e * a mid-term mock exercise that encourages students to test test their undertanding half way through the book, assess what they have learnt, utilize the primary literature and propose additional research applications and methods.\u003cbr\u003e \u003cbr\u003e * a supplementary Website, that includes:\u003cbr\u003e guidelines on searching the primary literature using internet and library search tools\u003cbr\u003e corrections,additions, and troubleshooting suggestions\u003cbr\u003e advice on; how to prepare laboratory notebooks, present oral reports, record keeping and report writing\u003cbr\u003e suggestions for future projects based on modified text protocols\u003cbr\u003e This book will be invaluable for undergraduate students of biology, biochemistry microbiology and biomedical sciences. It will also be useful for those students taking chemistry, chemical engineering and animal and plant science courses looking to improve their practical experience in the lab.\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47989615984869,"sku":"NP9780470843604","price":164.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9780470843604.jpg?v=1761784822","url":"https:\/\/k12savings.com\/products\/methods-in-molecular-biology-and-protein-chemistry-isbn-9780470843604","provider":"K12savings","version":"1.0","type":"link"}