{"product_id":"methods-in-biotechnology-isbn-9781119156789","title":"Methods in Biotechnology","description":"\u003cp\u003eAs rapid advances in biotechnology occur, there is a need for a pedagogical tool to aid current students and laboratory professionals in biotechnological methods; \u003ci\u003eMethods in Biotechnology\u003c\/i\u003e is an invaluable resource for those students and professionals.\u003c\/p\u003e \u003cp\u003e\u003ci\u003eMethods in Biotechnology\u003c\/i\u003e engages the reader by implementing an active learning approach, provided advanced study questions, as well as pre- and post-lab questions for each lab protocol. These self-directed study sections encourage the reader to not just perform experiments but to engage with the material on a higher level, utilizing critical thinking and troubleshooting skills.\u003c\/p\u003e This text is broken into three sections based on level – \u003ci\u003eMethods in Biotechnology, Advanced Methods in Biotechnology I, \u003c\/i\u003eand\u003ci\u003e Advanced Methods in Biotechnology II. \u003c\/i\u003eEach section contains 14-22 lab exercises, with instructor notes in appendices as well as an answer guide as a part of the book companion site. This text will be an excellent resource for both students and laboratory professionals in the biotechnology field.  \u003cp\u003ePreface vii\u003c\/p\u003e \u003cp\u003eAcknowledgements ix\u003c\/p\u003e \u003cp\u003eIntroduction to biotechnology lab xi\u003c\/p\u003e \u003cp\u003eAbout the companion website xix\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart I: Methods in Biotechnology (MB) Laboratory Exercises 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1 MB experiment 1: Lab measurements 3\u003c\/p\u003e \u003cp\u003e2 MB experiment 2: Use of the spectrophotometer and Beer’s law 9\u003c\/p\u003e \u003cp\u003e3 MB experiment 3: Making solutions and buffer efficacy 15\u003c\/p\u003e \u003cp\u003e4 MB experiment 4: Acid–base titration 19\u003c\/p\u003e \u003cp\u003e5 MB experiment 5: Protein denaturation and precipitation 23\u003c\/p\u003e \u003cp\u003e6 MB experiment 6: Bacterial transformation 27\u003c\/p\u003e \u003cp\u003e7 MB experiment 7: GFP purification 33\u003c\/p\u003e \u003cp\u003e8 MB experiment 8: SDS-PAGE analysis 37\u003c\/p\u003e \u003cp\u003e9 MB experiment 9: DNA isolation 41\u003c\/p\u003e \u003cp\u003e10 MB experiment 10: PCR-based \u003ci\u003eAlu\u003c\/i\u003e-human DNA typing 45\u003c\/p\u003e \u003cp\u003e11 MB experiment 11: Restriction enzyme digestion 51\u003c\/p\u003e \u003cp\u003e12 MB experiment 12: Agarose gel electrophoresis 53\u003c\/p\u003e \u003cp\u003e13 MB experiment 13: Ouchterlony and ELISA immunoassays 57\u003c\/p\u003e \u003cp\u003e14 MB experiment 14: Testing plant substances for antimicrobial activity 63\u003c\/p\u003e \u003cp\u003e15 MB experiment 15: Peroxidase enzyme activity assay 67\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart II: Advanced Methods in Biotechnology (AMB) 1 Laboratory Exercises 71\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e16 AMB 1 experiment 16: Aseptic technique and culture handling 73\u003c\/p\u003e \u003cp\u003e17 AMB 1 experiment 17: Yeast culture media preparation 77\u003c\/p\u003e \u003cp\u003e18 AMB 1 experiment 18: Growth curve 79\u003c\/p\u003e \u003cp\u003e19 AMB 1 experiment 19: Mini plasmid prep 83\u003c\/p\u003e \u003cp\u003e20 AMB 1 experiment 20: Restriction digestion, purification concentration and quantification of DNA 87\u003c\/p\u003e \u003cp\u003e21 AMB 1 experiment 21: Polymerase chain reaction (PCR) 91\u003c\/p\u003e \u003cp\u003e22 AMB 1 experiment 22: TA blunt end SLIC, and CPEC cloning of PCR product 97\u003c\/p\u003e \u003cp\u003e23 AMB 1 experiment 23: One-step multifragment assembly cloning 103\u003c\/p\u003e \u003cp\u003e24 AMB 1 experiment 24: Restriction enzyme digestion and fast agarose gel electrophoresis 111\u003c\/p\u003e \u003cp\u003e25 AMB 1 experiment 25: Southern blot transfer 117\u003c\/p\u003e \u003cp\u003e26 AMB 1 experiment 26: Probe labeling and purification 121\u003c\/p\u003e \u003cp\u003e27 AMB 1 experiment 27: Dot blot assay 125\u003c\/p\u003e \u003cp\u003e28 AMB 1 experiment 28: Pre-hybridization, hybridization and detection 127\u003c\/p\u003e \u003cp\u003e29 AMB 1 experiment 29: Total yeast RNA isolation and RT-PCR 131\u003c\/p\u003e \u003cp\u003e30 AMB 1 experiment 30: Yeast-based \u003ci\u003ein vivo \u003c\/i\u003erecombination cloning 139\u003c\/p\u003e \u003cp\u003e31 AMB 1 experiment 31: Plasmid DNA isolation from yeast 143\u003c\/p\u003e \u003cp\u003e32 AMB 1 experiment 32: \u003ci\u003eE. coli \u003c\/i\u003etransformation with yeast plasmid DNA 145\u003c\/p\u003e \u003cp\u003e33 AMB 1 experiment 33: X-gal filter lift assay 147\u003c\/p\u003e \u003cp\u003e34 AMB 1 experiment 34: Protein quantitation assay 149\u003c\/p\u003e \u003cp\u003e35 AMB 1 experiment 35: Quantitative 𝛃-Galactosidase assay in yeast 155\u003c\/p\u003e \u003cp\u003e36 AMB 1 experiment 36: Gel filtration chromatography (GFC) 161\u003c\/p\u003e \u003cp\u003e37 AMB 1 experiment 37: Ion exchange chromatography (IEC) 165\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart III: Advanced Methods in Biotechnology (AMB) 2 Laboratory Exercises 169\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e38 AMB 2 experiment 38: \u003ci\u003eE. coli \u003c\/i\u003eculture media preparation 171\u003c\/p\u003e \u003cp\u003e39 AMB 2 experiment 39: Site-directed mutagenesis 175\u003c\/p\u003e \u003cp\u003e40 AMB 2 experiment 40: Protein expression in \u003ci\u003eE. coli\u003c\/i\u003e 179\u003c\/p\u003e \u003cp\u003e41 AMB 2 experiment 41: Protein purification by affinity column chromatography 187\u003c\/p\u003e \u003cp\u003e42 AMB 2 experiment 42: SDS-PAGE analysis of affinity column fractions 193\u003c\/p\u003e \u003cp\u003e43 AMB 2 experiment 43: Western blot analysis of affinity column fractions 199\u003c\/p\u003e \u003cp\u003e44 AMB 2 experiment 44: Yeast media preparation and phenotypic analysis of yeast strains 203\u003c\/p\u003e \u003cp\u003e45 AMB 2 experiment 45: Yeast transformation for yeast two-hybrid (Y2H) assay and genome editing by CRISPR-Cas system 209\u003c\/p\u003e \u003cp\u003e46 AMB 2 experiment 46: Yeast mating-mediated Y2H assay and genomic PCR 215\u003c\/p\u003e \u003cp\u003e47 AMB 2 experiment 47: Yeast colony PCR screening and cycle DNA sequencing 221\u003c\/p\u003e \u003cp\u003e48 AMB 2 experiment 48: DNA sequencing electrophoresis 227\u003c\/p\u003e \u003cp\u003e49 AMB 2 experiment 49: RNA interference 237\u003c\/p\u003e \u003cp\u003e50 AMB 2 experiment 50: Protein preparation for 2D gel electrophoresis 241\u003c\/p\u003e \u003cp\u003e51 AMB 2 experiment 51: Two-dimensional gel electrophoresis 245\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart IV: Appendices 253\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1 Methods in Biotechnology Appendix 1 255\u003c\/p\u003e \u003cp\u003e2 MB Appendix 2 261\u003c\/p\u003e \u003cp\u003e3 MB Appendix 3 265\u003c\/p\u003e \u003cp\u003e4 MB Appendix 4 267\u003c\/p\u003e \u003cp\u003e5 AMB 1 Appendix 1 271\u003c\/p\u003e \u003cp\u003e6 AMB 1 Appendix 2 281\u003c\/p\u003e \u003cp\u003e7 AMB 1 Appendix 3 287\u003c\/p\u003e \u003cp\u003e8 AMB 1 Appendix 4 291\u003c\/p\u003e \u003cp\u003e9 AMB 1 Appendix 5 297\u003c\/p\u003e \u003cp\u003e10 AMB 2 Appendix 1 299\u003c\/p\u003e \u003cp\u003e11 AMB 2 Appendix 2 307\u003c\/p\u003e \u003cp\u003e12 AMB 2 Appendix 3 313\u003c\/p\u003e \u003cp\u003e13 AMB 2 Appendix 4 317\u003c\/p\u003e \u003cp\u003e14 AMB 2 Appendix 5 319\u003c\/p\u003e \u003cp\u003e15 AMB 2 Appendix 6 321\u003c\/p\u003e \u003cp\u003e16 AMB 2 Appendix 7 325\u003c\/p\u003e \u003cp\u003eGlossary 327\u003c\/p\u003e \u003cp\u003eAbbreviations 335\u003c\/p\u003e \u003cp\u003eIndex 337\u003c\/p\u003e  \u003cp\u003e\u003cb\u003eSEUNG-BEOM HONG\u003c\/b\u003e is a Visiting Assistant Professor of Biotechnology at the University of Houston-Clear Lake in Houston, TX, USA. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eM. BAZLUR RASHID\u003c\/b\u003e is an Associate Professor of Biology and Biotechnology and former Program Chair of Biotechnology at the University of Houston-Clear Lake in Houston, TX, USA. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eLORY Z. SANTIAGO-VÁZQUEZ\u003c\/b\u003e is an Associate Professor of Biotechnology at the University of Houston-Clear Lake in Houston, TX, USA.   \u003c\/p\u003e\u003cp\u003e\u003cb\u003eMETHODS IN BIOTECHNOLOGY\u003c\/b\u003e \u003c\/p\u003e\u003cp\u003eAs rapid advances in biotechnology occur, there is a need for a pedagogical tool to aid current students and laboratory professionals in biotechnological methods. \u003ci\u003eMethods in Biotechnology\u003c\/i\u003e is an invaluable resource for those students and professionals. \u003c\/p\u003e\u003cp\u003e\u003ci\u003eMethods in Biotechnology\u003c\/i\u003e engages the reader by implementing an active learning approach, provides advanced study questions, as well as pre- and post-lab questions for each lab protocol. These self-directed study sections encourage the reader to not just perform experiments but to engage with the material on a higher level, utilizing critical thinking and troubleshooting skills. \u003c\/p\u003e\u003cp\u003eThis text is broken into three sections based on level – \u003ci\u003eMethods in Biotechnology, Advanced Methods in Biotechnology 1\u003c\/i\u003e, and \u003ci\u003eAdvanced Methods in Biotechnology 2.\u003c\/i\u003e Each section contains 14 to 22 lab experiments, with instructor notes in appendices as well as an answer guide as a part of the book companion site. This text will be an excellent resource for both students and laboratory professionals in the biotechnology field. \u003c\/p\u003e\u003cul\u003e \u003cli\u003e Uniquely implements a holistic approach to biotechnology that lends itself well to use for both instruction and research in advanced biotechnology laboratories\u003c\/li\u003e \u003cli\u003e Provides a scheduled framework for students learning advanced laboratory techniques and how to design experiments and interpret results\u003c\/li\u003e \u003cli\u003e Includes basic biochemical techniques and bioinformatics exercises, as well as laboratory mathematics practice problem sets (with answer keys)\u003c\/li\u003e \u003cli\u003e Encourages students to engage in a higher level of critical thinking and troubleshooting, rather than simply performing experiments\u003c\/li\u003e \u003cli\u003e Includes access to a companion website hosting book content including figures, tables, etc.\u003c\/li\u003e \u003c\/ul\u003e","brand":"Wiley-Blackwell","offers":[{"title":"Default Title","offer_id":47989615853797,"sku":"NP9781119156789","price":118.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9781119156789.jpg?v=1761784822","url":"https:\/\/k12savings.com\/products\/methods-in-biotechnology-isbn-9781119156789","provider":"K12savings","version":"1.0","type":"link"}