{"product_id":"mass-spectrometry-isbn-9780471713951","title":"Mass Spectrometry","description":"With contributions from noted experts from Europe and North America, \u003ci\u003eMass Spectrometry Instrumentation, Interpretation, and Applications\u003c\/i\u003e serves as a forum to introduce students to the whole world of mass spectrometry and to the many different perspectives that each scientific field brings to its use. The book emphasizes the use of this important analytical technique in many different fields, including applications for organic and inorganic chemistry, forensic science, biotechnology, and many other areas. After describing the history of mass spectrometry, the book moves on to discuss instrumentation, theory, and basic applications. \u003cp\u003eForeword xiii\u003c\/p\u003e \u003cp\u003eContributors xv\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart I Instrumentation 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Definitions and Explanations 3\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eAnn Westman-Brinkmalm and Gunnar Brinkmalm\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eReferences 13\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 A Mass Spectrometer’s Building Blocks 15\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eAnn Westman-Brinkmalm and Gunnar Brinkmalm\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2.1. Ion Sources 15\u003c\/p\u003e \u003cp\u003e2.1.1. Gas Discharge 16\u003c\/p\u003e \u003cp\u003e2.1.2. Thermal Ionization 16\u003c\/p\u003e \u003cp\u003e2.1.3. Spark Source 19\u003c\/p\u003e \u003cp\u003e2.1.4. Glow Discharge 20\u003c\/p\u003e \u003cp\u003e2.1.5. Inductively Coupled Plasma 21\u003c\/p\u003e \u003cp\u003e2.1.6. Electron Ionization 23\u003c\/p\u003e \u003cp\u003e2.1.7. Chemical Ionization 24\u003c\/p\u003e \u003cp\u003e2.1.8. Atmospheric Pressure Chemical Ionization 24\u003c\/p\u003e \u003cp\u003e2.1.9. Photoionization 25\u003c\/p\u003e \u003cp\u003e2.1.10. Multiphoton Ionization 25\u003c\/p\u003e \u003cp\u003e2.1.11. Atmospheric Pressure Photoionization 26\u003c\/p\u003e \u003cp\u003e2.1.12. Field Ionization 26\u003c\/p\u003e \u003cp\u003e2.1.13. Field Desorption 27\u003c\/p\u003e \u003cp\u003e2.1.14. Thermospray Ionization 27\u003c\/p\u003e \u003cp\u003e2.1.15. Electrospray Ionization 27\u003c\/p\u003e \u003cp\u003e2.1.16. Desorption Electrospray Ionization 29\u003c\/p\u003e \u003cp\u003e2.1.17. Direct Analysis in Real Time 30\u003c\/p\u003e \u003cp\u003e2.1.18. Secondary Ion Mass Spectrometry 31\u003c\/p\u003e \u003cp\u003e2.1.19. Fast Atom Bombardment 33\u003c\/p\u003e \u003cp\u003e2.1.20. Plasma Desorption 34\u003c\/p\u003e \u003cp\u003e2.1.21. Laser Desorption\/Ionization 34\u003c\/p\u003e \u003cp\u003e2.1.22. Matrix-Assisted Laser Desorption\/Ionization 35\u003c\/p\u003e \u003cp\u003e2.1.23. Atmospheric Pressure Matrix-Assisted Laser Desorption\/Ionization 37\u003c\/p\u003e \u003cp\u003e2.2. Mass Analyzers 38\u003c\/p\u003e \u003cp\u003e2.2.1. Time-of-Flight 40\u003c\/p\u003e \u003cp\u003e2.2.2. Magnetic\/Electric Sector 45\u003c\/p\u003e \u003cp\u003e2.2.3. Quadrupole Mass Filter 49\u003c\/p\u003e \u003cp\u003e2.2.4. Quadrupole Ion Trap 51\u003c\/p\u003e \u003cp\u003e2.2.5. Orbitrap 55\u003c\/p\u003e \u003cp\u003e2.2.6. Fourier Transform Ion Cyclotron Resonance 58\u003c\/p\u003e \u003cp\u003e2.2.7. Accelerator Mass Spectrometry 62\u003c\/p\u003e \u003cp\u003e2.3. Detectors 65\u003c\/p\u003e \u003cp\u003e2.3.1. Photoplate Detector 65\u003c\/p\u003e \u003cp\u003e2.3.2. Faraday Detector 67\u003c\/p\u003e \u003cp\u003e2.3.3. Electron Multipliers 67\u003c\/p\u003e \u003cp\u003e2.3.4. Focal Plane Detector 69\u003c\/p\u003e \u003cp\u003e2.3.5. Scintillation Detector 69\u003c\/p\u003e \u003cp\u003e2.3.6. Cryogenic Detector 70\u003c\/p\u003e \u003cp\u003e2.3.7. Solid-State Detector 70\u003c\/p\u003e \u003cp\u003e2.3.8. Image Current Detection 70\u003c\/p\u003e \u003cp\u003eReferences 71\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Tandem Mass Spectrometry 89\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eAnn Westman-Brinkmalm and Gunnar Brinkmalm\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3.1. Tandem MS Analyzer Combinations 91\u003c\/p\u003e \u003cp\u003e3.1.1. Tandem-in-Space 91\u003c\/p\u003e \u003cp\u003e3.1.2. Tandem-in-Time 95\u003c\/p\u003e \u003cp\u003e3.1.3. Other Tandem MS Configurations 97\u003c\/p\u003e \u003cp\u003e3.2. Ion Activation Methods 97\u003c\/p\u003e \u003cp\u003e3.2.1. In-Source Decay 97\u003c\/p\u003e \u003cp\u003e3.2.2. Post-Source Decay 98\u003c\/p\u003e \u003cp\u003e3.2.3. Collision Induced\/Activated Dissociation 98\u003c\/p\u003e \u003cp\u003e3.2.4. Photodissociation 100\u003c\/p\u003e \u003cp\u003e3.2.5. Blackbody Infrared Radiative Dissociation 100\u003c\/p\u003e \u003cp\u003e3.2.6. Electron Capture Dissociation 101\u003c\/p\u003e \u003cp\u003e3.2.7. Electron Transfer Dissociation 101\u003c\/p\u003e \u003cp\u003e3.2.8. Surface-Induced Dissociation 101\u003c\/p\u003e \u003cp\u003eReferences 102\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Separation Methods 105\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eAnn Westman-Brinkmalm, Jerzy Silberring, and Gunnar Brinkmalm\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e4.1. Chromatography 106\u003c\/p\u003e \u003cp\u003e4.1.1. Gas Chromatography 106\u003c\/p\u003e \u003cp\u003e4.1.2. Liquid Chromatography 107\u003c\/p\u003e \u003cp\u003e4.1.3. Supercritical Fluid Chromatography 109\u003c\/p\u003e \u003cp\u003e4.2. Electric-Field Driven Separations 110\u003c\/p\u003e \u003cp\u003e4.2.1. Ion Mobility 110\u003c\/p\u003e \u003cp\u003e4.2.2. Electrophoresis 111\u003c\/p\u003e \u003cp\u003eReferences 113\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart II Interpretation 117\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Introduction to Mass Spectra Interpretation: Organic Chemistry 119\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eAlbert T. Lebedev\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5.1. Basic Concepts 119\u003c\/p\u003e \u003cp\u003e5.2. Inlet Systems 121\u003c\/p\u003e \u003cp\u003e5.2.1. Direct Inlet 121\u003c\/p\u003e \u003cp\u003e5.2.2. Chromatography-Mass Spectrometry 121\u003c\/p\u003e \u003cp\u003e5.3. Physical Bases of Mass Spectrometry 128\u003c\/p\u003e \u003cp\u003e5.3.1. Electron Ionization 129\u003c\/p\u003e \u003cp\u003e5.3.2. Basics of Fragmentation Processes in Mass Spectrometry 130\u003c\/p\u003e \u003cp\u003e5.3.3. Metastable Ions 135\u003c\/p\u003e \u003cp\u003e5.4. Theoretical Rules and Approaches to Interpret Mass Spectra 137\u003c\/p\u003e \u003cp\u003e5.4.1. Stability of Charged and Neutral Particles 137\u003c\/p\u003e \u003cp\u003e5.4.2. The Concept of Charge and Unpaired Electron Localization 148\u003c\/p\u003e \u003cp\u003e5.4.3. Charge Remote Fragmentation 151\u003c\/p\u003e \u003cp\u003e5.5. Practical Approaches to Interpret Mass Spectra 152\u003c\/p\u003e \u003cp\u003e5.5.1. Molecular Ion 152\u003c\/p\u003e \u003cp\u003e5.5.2. High Resolution Mass Spectrometry 155\u003c\/p\u003e \u003cp\u003e5.5.3. Determination of the Elemental Composition of Ions on the Basis of Isotopic Peaks 158\u003c\/p\u003e \u003cp\u003e5.5.4. The Nitrogen Rule 164\u003c\/p\u003e \u003cp\u003e5.5.5. Establishing the 13 C Isotope Content in Natural Samples 166\u003c\/p\u003e \u003cp\u003e5.5.6. Calculation of the Isotopic Purity of Samples 166\u003c\/p\u003e \u003cp\u003e5.5.7. Fragment Ions 168\u003c\/p\u003e \u003cp\u003e5.5.8. Mass Spectral Libraries 173\u003c\/p\u003e \u003cp\u003e5.5.9. Additional Mass Spectral Information 173\u003c\/p\u003e \u003cp\u003e5.5.10. Fragmentation Scheme 175\u003c\/p\u003e \u003cp\u003eReferences 177\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Sequencing of Peptides and Proteins 179\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eMarek Noga, Tomasz Dylag, and Jerzy Silberring\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6.1. Basic Concepts 179\u003c\/p\u003e \u003cp\u003e6.2. Tandem Mass Spectrometry of Peptides and Proteins 181\u003c\/p\u003e \u003cp\u003e6.3. Peptide Fragmentation Nomenclature 183\u003c\/p\u003e \u003cp\u003e6.3.1. Roepstorff’s Nomenclature 183\u003c\/p\u003e \u003cp\u003e6.3.2. Biemann’s Nomenclature 185\u003c\/p\u003e \u003cp\u003e6.3.3. Cyclic Peptides 187\u003c\/p\u003e \u003cp\u003e6.4. Technical Aspects and Fragmentation Rules 188\u003c\/p\u003e \u003cp\u003e6.5. Why Peptide Sequencing? 190\u003c\/p\u003e \u003cp\u003e6.6. De Novo Sequencing 192\u003c\/p\u003e \u003cp\u003e6.6.1. Data Acquisition 193\u003c\/p\u003e \u003cp\u003e6.6.2. Sequencing Procedure Examples 194\u003c\/p\u003e \u003cp\u003e6.6.3. Tips and Tricks 205\u003c\/p\u003e \u003cp\u003e6.7. Peptide Derivatization Prior to Fragmentation 207\u003c\/p\u003e \u003cp\u003e6.7.1. Simplification of Fragmentation Patterns 208\u003c\/p\u003e \u003cp\u003e6.7.2. Stable Isotopes Labeling 209\u003c\/p\u003e \u003cp\u003eAcknowledgments 210\u003c\/p\u003e \u003cp\u003eReferences 210\u003c\/p\u003e \u003cp\u003eOnline Tutorials 210\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Optimizing Sensitivity and Specificity in Mass Spectrometric Proteome Analysis 211\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJan Eriksson and David Fenyö\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7.1. Quantitation 212\u003c\/p\u003e \u003cp\u003e7.2. Peptide and Protein Identification 213\u003c\/p\u003e \u003cp\u003e7.3. Success Rate and Relative Dynamic Range 218\u003c\/p\u003e \u003cp\u003e7.4. Summary 220\u003c\/p\u003e \u003cp\u003eReferences 220\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart III Applications 223\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Doping Control 225\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eGraham Trout\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eReferences 233\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Oceanography 235\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eR. Timothy Short, Robert H. Byrne, David Hollander, Johan Schijf, Strawn K. Toler, and Edward S. VanVleet\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eReferences 241\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 “omics” Applications 243\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eSimone König\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e10.1. Introduction 243\u003c\/p\u003e \u003cp\u003e10.2. Genomics and Transcriptomics 246\u003c\/p\u003e \u003cp\u003e10.3. Proteomics 248\u003c\/p\u003e \u003cp\u003e10.4. Metabolomics 251\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Space Sciences 253\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eRobert Sheldon\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e11.1. Introduction 253\u003c\/p\u003e \u003cp\u003e11.2. Origins 254\u003c\/p\u003e \u003cp\u003e11.3. Dynamics 256\u003c\/p\u003e \u003cp\u003e11.4. The Space MS Paradox 257\u003c\/p\u003e \u003cp\u003e11.5. A Brief History of Space MS 259\u003c\/p\u003e \u003cp\u003e11.5.1. Beginnings 259\u003c\/p\u003e \u003cp\u003e11.5.2. Linear TOF-MS 260\u003c\/p\u003e \u003cp\u003e11.5.3. Isochronous TOF-MS 262\u003c\/p\u003e \u003cp\u003e11.6. GENESIS and the Future 264\u003c\/p\u003e \u003cp\u003eReferences 264\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Bioterrorism 267\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eVito G. DelVecchio and Cesar V. Mujer\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e12.1. What is Bioterrorism? 267\u003c\/p\u003e \u003cp\u003e12.2. Some Historical Accounts of Bioterrorism 267\u003c\/p\u003e \u003cp\u003e12.3. Geneva Protocol of 1925 and Biological Weapons Convention of 1972 268\u003c\/p\u003e \u003cp\u003e12.4. Categories of Biothreat Agents 268\u003c\/p\u003e \u003cp\u003e12.5. Challenges 269\u003c\/p\u003e \u003cp\u003e12.6. MS Identification of Biomarker Proteins 270\u003c\/p\u003e \u003cp\u003e12.7. Development of New Therapeutics and Vaccines Using Immunoproteomics 271\u003c\/p\u003e \u003cp\u003eReferences 272\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Imaging of Small Molecules 275\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eMałgorzata Iwona Szynkowska\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e13.1. SIMS Imaging 277\u003c\/p\u003e \u003cp\u003e13.2. Biological Applications (Cells, Tissues, and Pharmaceuticals) 278\u003c\/p\u003e \u003cp\u003e13.3. Catalysis 280\u003c\/p\u003e \u003cp\u003e13.4. Forensics 281\u003c\/p\u003e \u003cp\u003e13.5. Semiconductors 282\u003c\/p\u003e \u003cp\u003e13.6. The Future 283\u003c\/p\u003e \u003cp\u003eReferences 285\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 Utilization of Mass Spectrometry In Clinical Chemistry 287\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eDonald H. Chace\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e14.1. Introduction 287\u003c\/p\u003e \u003cp\u003e14.2. Where are Mass Spectrometers Utilized in Clinical Applications? 288\u003c\/p\u003e \u003cp\u003e14.3. Most Common Analytes Detected by Mass Spectrometers 288\u003c\/p\u003e \u003cp\u003e14.4. Multianalyte Detection of Clinical Biomarkers, The Real Success Story 289\u003c\/p\u003e \u003cp\u003e14.5. Quantitative Profiling 291\u003c\/p\u003e \u003cp\u003e14.6. A Clinical Example of the Use of Mass Spectrometry 292\u003c\/p\u003e \u003cp\u003e14.7. Demonstrations of Concepts of Quantification in Clinical Chemistry 294\u003c\/p\u003e \u003cp\u003e14.7.1. Tandem Mass Spectrometry and Sorting (Pocket Change) 294\u003c\/p\u003e \u003cp\u003e14.7.2. Isotope Dilution and Quantification (the Jelly Bean Experiment) 295\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 Polymers 299\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eMaurizio S. Montaudo\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e15.1. Introduction 299\u003c\/p\u003e \u003cp\u003e15.2. Instrumentation, Sample Preparation, and Matrices 300\u003c\/p\u003e \u003cp\u003e15.3. Analysis of Ultrapure Polymer Samples 301\u003c\/p\u003e \u003cp\u003e15.4. Analysis of Polymer Samples in which all Chains Possess the Same Backbone 301\u003c\/p\u003e \u003cp\u003e15.5. Analysis of Polymer Mixtures with Different Backbones 303\u003c\/p\u003e \u003cp\u003e15.6. Determination of Average Molar Masses 303\u003c\/p\u003e \u003cp\u003eReferences 306\u003c\/p\u003e \u003cp\u003e\u003cb\u003e16 Forensic Sciences 309\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eMaria Kala\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e16.1. Introduction 309\u003c\/p\u003e \u003cp\u003e16.2. Materials Examined and Goals of Analysis 311\u003c\/p\u003e \u003cp\u003e16.3. Sample Preparation 312\u003c\/p\u003e \u003cp\u003e16.4. Systematic Toxicological Analysis 312\u003c\/p\u003e \u003cp\u003e16.4.1. GC-MS Procedures 315\u003c\/p\u003e \u003cp\u003e16.4.2. LC-MS Procedures 315\u003c\/p\u003e \u003cp\u003e16.5. Quantitative Analysis 317\u003c\/p\u003e \u003cp\u003e16.6. Identification of Arsons 319\u003c\/p\u003e \u003cp\u003eReferences 319\u003c\/p\u003e \u003cp\u003e\u003cb\u003e17 New Approaches to Neurochemistry 321\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJonas Bergquist, Jerzy Silberring, and Rolf Ekman\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e17.1. Introduction 321\u003c\/p\u003e \u003cp\u003e17.2. Why is there so Little Research in this Area? 322\u003c\/p\u003e \u003cp\u003e17.3. Proteomics and Neurochemistry 323\u003c\/p\u003e \u003cp\u003e17.3.1. The Synapse 324\u003c\/p\u003e \u003cp\u003e17.3.2. Learning and Memory 324\u003c\/p\u003e \u003cp\u003e17.3.3. The Brain and the Immune System 325\u003c\/p\u003e \u003cp\u003e17.3.4. Stress and Anxiety 327\u003c\/p\u003e \u003cp\u003e17.3.5. Psychiatric Diseases and Disorders 329\u003c\/p\u003e \u003cp\u003e17.3.6. Chronic Fatigue Syndrome 329\u003c\/p\u003e \u003cp\u003e17.3.7. Addiction 330\u003c\/p\u003e \u003cp\u003e17.3.8. Pain 331\u003c\/p\u003e \u003cp\u003e17.3.9. Neurodegenerative Diseases 331\u003c\/p\u003e \u003cp\u003e17.4. Conclusions 333\u003c\/p\u003e \u003cp\u003eAcknowledgments 333\u003c\/p\u003e \u003cp\u003eReferences 334\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart IV Appendix 337\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIndex 353\u003c\/p\u003e \"It was my great pleasure to read this clearly written and well organized mass spectrometry (MS) book. In view, it can serve as an excellent textbook for both undergraduate and graduate students who major in analytical, biological, forensic, or environmental chemistry, as well as a valuable resource to those researchers who are interested in the MS-based chemical analysis.\" (J Am Soc Mass Spectrom, 2011)  \u003cp\u003e \"The book is particularly designed for graduate students, with the assumption being made that most of them will not become mass spectrometry specialists. Instead, it focuses on how they can use the technique to support and advance research across a broad range of disciplines.\" (Chemistry Journals, 11 April 2011)\u003c\/p\u003e  \u003cb\u003eRolf Ekman, PhD\u003c\/b\u003e, is a Professor of Neurochemistry at University of Gothenburg in Sweden.  \u003cp\u003e\u003cb\u003eJERZY SILBERRING,\u003c\/b\u003e PhD, is the Head of the Department of Neurobiochemistry in the Department of Chemistry and the former deputy head of the Regional Laboratory of Physicochemical Analyses at Jagiellonian University in Krakow, Poland.\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAnn M. Westman-Brinkmalm, PhD\u003c\/b\u003e, is a Junior Research Fellow at the Sahlgrenska Academy at University of Gothenburg in Sweden.\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAgnieszka Kraj, PhD\u003c\/b\u003e, is an Assistant Professor in the Department of Neurobiochemistry, Faculty of Chemistry at Jagiellonian University in Krakow, Poland.\u003c\/p\u003e  Helps students fully leverage mass spectrometry in whichever field of research they choose  \u003cp\u003e\u003ci\u003eMass Spectrometry: Instrumentation, Interpretation, and Applications\u003c\/i\u003e enables students to become fully versed in the principles and uses of mass spectrometry. Featuring contributions from international experts, the text introduces the many perspectives and approaches that different scientific fields bring to mass spectrometry, including applications for organic and inorganic chemistry, forensic science, biotechnology, and much more. This multidisciplinary approach enables students to apply their knowledge in their chosen fields of research in order to identify, quantify, and determine the structures and chemical properties of compounds.\u003c\/p\u003e \u003cp\u003eThis text is divided into three parts that guide students from basic principles to applications:\u003c\/p\u003e \u003cul\u003e \u003cli\u003e \u003cp\u003e\u003cb\u003ePart One, Instrumentation,\u003c\/b\u003e begins with basic definitions and explanations followed by a discussion of the mass spectrometer and its building blocks. Next, the text describes fragmentation methods and tandem MS analyzer configurations, ending with a short summary of separation methods used in conjunction with mass spectrometry.\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003e\u003cb\u003ePart Two, Interpretation,\u003c\/b\u003e explains basic concepts in mass spectra interpretation and then demonstrates how these concepts are used to interpret mass spectra in organic chemistry. Students also learn how to use mass spectrometry as a tool for peptide sequencing and how to optimize sensitivity and specificity in mass spectrometric proteome analysis.\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003e\u003cb\u003ePart Three,\u003c\/b\u003e Applications, features ten researchers and research groups from different fields describing how they use mass spectrometry in their own work.\u003c\/p\u003e \u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eDesigned for graduate-level students, this textbook assumes that most students will not become mass spectrometry specialists. Instead, it focuses on how they can use the mass spectrometer to support and advance research across a broad range of disciplines.\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47989579448549,"sku":"NP9780471713951","price":166.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9780471713951.jpg?v=1761784676","url":"https:\/\/k12savings.com\/products\/mass-spectrometry-isbn-9780471713951","provider":"K12savings","version":"1.0","type":"link"}