{"product_id":"introduction-to-modern-liquid-chromatography-isbn-9780470167540","title":"Introduction to Modern Liquid Chromatography","description":"\u003cb\u003eThe latest edition of the authoritative reference to HPLC\u003c\/b\u003e  \u003cp\u003eHigh-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and\/or purify virtually any sample. Snyder and Kirkland's Introduction to \u003ci\u003eModern Liquid Chromatography\u003c\/i\u003e has long represented the premier reference to HPLC. This \u003ci\u003eThird Edition\u003c\/i\u003e, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples.\u003c\/p\u003e \u003cp\u003eThis carefully considered \u003ci\u003eThird Edition\u003c\/i\u003e maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as:\u003c\/p\u003e \u003cul\u003e \u003cli\u003eThe basis of HPLC separation and the general effects of different experimental conditions\u003c\/li\u003e \u003cli\u003eEquipment and detection\u003c\/li\u003e \u003cli\u003eThe column—the \"heart\" of the HPLC system\u003c\/li\u003e \u003cli\u003eReversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques\u003c\/li\u003e \u003cli\u003eComputer simulation, qualitative and quantitative analysis, and method validation and quality control\u003c\/li\u003e \u003cli\u003eThe separation of large molecules, including both biological and synthetic polymers\u003c\/li\u003e \u003cli\u003eChiral separations, preparative separations, and sample preparation\u003c\/li\u003e \u003cli\u003eSystematic development of HPLC separations—new to this edition\u003c\/li\u003e \u003cli\u003eTroubleshooting tricks, techniques, and case studies for both equipment and chromatograms\u003c\/li\u003e \u003c\/ul\u003e Designed to fulfill the needs of the full range of HPLC users, from novices to experts, \u003ci\u003eIntroduction to Modern Liquid Chromatography\u003c\/i\u003e, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available. \u003cp\u003ePreface xxxi\u003c\/p\u003e \u003cp\u003eGlossary of Symbols and Abbreviations xxxv\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Introduction 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 Background Information 2\u003c\/p\u003e \u003cp\u003e1.2 A Short History of HPLC 6\u003c\/p\u003e \u003cp\u003e1.3 Some Alternatives to HPLC 8\u003c\/p\u003e \u003cp\u003e1.4 Other Sources of HPLC Information 12\u003c\/p\u003e \u003cp\u003eReferences 15\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Basic Concepts and the Control of Separation 19\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 Introduction 20\u003c\/p\u003e \u003cp\u003e2.2 The Chromatographic Process 20\u003c\/p\u003e \u003cp\u003e2.3 Retention 24\u003c\/p\u003e \u003cp\u003e2.4 Peak Width and the Column Plate Number N 35\u003c\/p\u003e \u003cp\u003e2.5 Resolution and Method Development 54\u003c\/p\u003e \u003cp\u003e2.6 Sample Size Effects 69\u003c\/p\u003e \u003cp\u003e2.7 Related Topics 74\u003c\/p\u003e \u003cp\u003eReferences 83\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Equipment 87\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introduction 88\u003c\/p\u003e \u003cp\u003e3.2 Reservoirs and Solvent Filtration 89\u003c\/p\u003e \u003cp\u003e3.3 Mobile-Phase Degassing 92\u003c\/p\u003e \u003cp\u003e3.4 Tubing and Fittings 96\u003c\/p\u003e \u003cp\u003e3.5 Pumping Systems 104\u003c\/p\u003e \u003cp\u003e3.6 Autosamplers 113\u003c\/p\u003e \u003cp\u003e3.7 Column Ovens 125\u003c\/p\u003e \u003cp\u003e3.8 Data Systems 127\u003c\/p\u003e \u003cp\u003e3.9 Extra-Column Effects 131\u003c\/p\u003e \u003cp\u003e3.10 Maintenance 131\u003c\/p\u003e \u003cp\u003eReferences 144\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Detection 147\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Introduction 148\u003c\/p\u003e \u003cp\u003e4.2 Detector Characteristics 149\u003c\/p\u003e \u003cp\u003e4.3 Introduction to Individual Detectors 160\u003c\/p\u003e \u003cp\u003e4.4 UV-Visible Detectors 160\u003c\/p\u003e \u003cp\u003e4.5 Fluorescence Detectors 167\u003c\/p\u003e \u003cp\u003e4.6 Electrochemical (Amperometric) Detectors 170\u003c\/p\u003e \u003cp\u003e4.7 Radioactivity Detectors 172\u003c\/p\u003e \u003cp\u003e4.8 Conductivity Detectors 174\u003c\/p\u003e \u003cp\u003e4.9 Chemiluminescent Nitrogen Detector 174\u003c\/p\u003e \u003cp\u003e4.10 Chiral Detectors 175\u003c\/p\u003e \u003cp\u003e4.11 Refractive Index Detectors 177\u003c\/p\u003e \u003cp\u003e4.12 Light-Scattering Detectors 180\u003c\/p\u003e \u003cp\u003e4.13 Corona-Discharge Detector (CAD) 184\u003c\/p\u003e \u003cp\u003e4.14 Mass Spectral Detectors (MS) 185\u003c\/p\u003e \u003cp\u003e4.15 Other Hyphenated Detectors 191\u003c\/p\u003e \u003cp\u003e4.16 Sample Derivatization and Reaction Detectors 194\u003c\/p\u003e \u003cp\u003eReferences 196\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 The Column 199\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction 200\u003c\/p\u003e \u003cp\u003e5.2 Column Supports 200\u003c\/p\u003e \u003cp\u003e5.3 Stationary Phases 217\u003c\/p\u003e \u003cp\u003e5.4 Column Selectivity 227\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e5.5 Column Hardware 238\u003c\/p\u003e \u003cp\u003e5.6 Column-Packing Methods 240\u003c\/p\u003e \u003cp\u003e5.7 Column Specifications 244\u003c\/p\u003e \u003cp\u003e5.8 Column Handling 246\u003c\/p\u003e \u003cp\u003eReferences 250\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Reversed-phase Chromatography for Neutral Sam- Ples 253\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction 254\u003c\/p\u003e \u003cp\u003e6.2 Retention 256\u003c\/p\u003e \u003cp\u003e6.3 Selectivity 263\u003c\/p\u003e \u003cp\u003e6.4 Method Development and Strategies for Optimizing Selectivity 284\u003c\/p\u003e \u003cp\u003e6.5 Nonaqueous Reversed-Phase Chromatography (narp) 295\u003c\/p\u003e \u003cp\u003e6.6 Special Problems 297\u003c\/p\u003e \u003cp\u003eReferences 298\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Ionic Samples: Reversed-phase Ion-pair and Ion- Exchange Chromatography 303\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction 304\u003c\/p\u003e \u003cp\u003e7.2 Acid–Base Equilibria and Reversed-Phase Retention 304\u003c\/p\u003e \u003cp\u003e7.3 Separation of Ionic Samples by Reversed-Phase Chromatography (RPC) 319\u003c\/p\u003e \u003cp\u003e7.4 Ion-Pair Chromatography (IPC) 331\u003c\/p\u003e \u003cp\u003e7.5 Ion-Exchange Chromatography (IEC) 349\u003c\/p\u003e \u003cp\u003eReferences 357\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Normal-phase Chromatography 361\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction 362\u003c\/p\u003e \u003cp\u003e8.2 Retention 363\u003c\/p\u003e \u003cp\u003e8.3 Selectivity 376\u003c\/p\u003e \u003cp\u003e8.4 Method-Development Summary 385\u003c\/p\u003e \u003cp\u003e8.5 Problems in the Use of NPC 392\u003c\/p\u003e \u003cp\u003e8.6 Hydrophilic Interaction Chromatography (HILIC) 395\u003c\/p\u003e \u003cp\u003eReferences 401\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Gradient Elution 403\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction 404\u003c\/p\u003e \u003cp\u003e9.2 Experimental Conditions and Their Effects on Separation 412\u003c\/p\u003e \u003cp\u003e9.3 Method Development 434\u003c\/p\u003e \u003cp\u003e9.4 Large-Molecule Separations 464\u003c\/p\u003e \u003cp\u003e9.5 Other Separation Modes 465\u003c\/p\u003e \u003cp\u003e9.6 Problems 470\u003c\/p\u003e \u003cp\u003eReferences 471\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Computer-assisted Method Development 475\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e10.1 Introduction 475\u003c\/p\u003e \u003cp\u003e10.2 Computer-Simulation Software 481\u003c\/p\u003e \u003cp\u003e10.3 Other Method-Development Software 491\u003c\/p\u003e \u003cp\u003e10.4 Computer Simulation and Method Development 492\u003c\/p\u003e \u003cp\u003eReferences 497\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Qualitative and Quantitative Analysis 499\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e11.1 Introduction 499\u003c\/p\u003e \u003cp\u003e11.2 Signal Measurement 500\u003c\/p\u003e \u003cp\u003e11.3 Qualitative Analysis 516\u003c\/p\u003e \u003cp\u003e11.4 Quantitative Analysis 520\u003c\/p\u003e \u003cp\u003e11.5 Summary 529\u003c\/p\u003e \u003cp\u003eReferences 529\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Method Validation 531\u003cbr\u003e \u003c\/b\u003ewith \u003ci\u003eMichael Swartz\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction 532\u003c\/p\u003e \u003cp\u003e12.2 Terms and Definitions 534\u003c\/p\u003e \u003cp\u003e12.3 System Suitability 542\u003c\/p\u003e \u003cp\u003e12.4 Documentation 543\u003c\/p\u003e \u003cp\u003e12.5 Validation for Different Pharmaceutical-Method Types 546\u003c\/p\u003e \u003cp\u003e12.6 Bioanalytical Methods 548\u003c\/p\u003e \u003cp\u003e12.7 Analytical Method Transfer (AMT) 554\u003c\/p\u003e \u003cp\u003e12.8 Method Adjustment or Method Modification 561\u003c\/p\u003e \u003cp\u003e12.9 Quality Control and Quality Assurance 564\u003c\/p\u003e \u003cp\u003e12.10 Summary 565\u003c\/p\u003e \u003cp\u003eReferences 566\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Biochemical and Synthetic Polymer Separations 569\u003cbr\u003e \u003c\/b\u003ewith \u003ci\u003eTimothy Wehr, Carl Scandella, \u003c\/i\u003eand\u003ci\u003e Peter Schoenmakers\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e13.1 Biomacromolecules 570\u003c\/p\u003e \u003cp\u003e13.2 Molecular Structure and Conformation 571\u003c\/p\u003e \u003cp\u003e13.3 Special Considerations for Biomolecule HPLC 579\u003c\/p\u003e \u003cp\u003e13.4 Separation of Peptides and Proteins 584\u003c\/p\u003e \u003cp\u003e13.5 Separation of Nucleic Acids 618\u003c\/p\u003e \u003cp\u003e13.6 Separation of Carbohydrates 625\u003c\/p\u003e \u003cp\u003e13.7 Separation of Viruses 630\u003c\/p\u003e \u003cp\u003e13.8 Size-Exclusion Chromatography (SEC) 631\u003c\/p\u003e \u003cp\u003e13.9 Large-Scale Purification of Large Biomolecules 641\u003c\/p\u003e \u003cp\u003e13.10 Synthetic Polymers 648\u003c\/p\u003e \u003cp\u003eReferences 658\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 Enantiomer Separations 665 \u003cbr\u003e \u003c\/b\u003ewith \u003ci\u003eMichael Lämmerhofer, Norbert M. Maier\u003c\/i\u003e and \u003ci\u003eWolfgang Lindner\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e14.1 Introduction 666\u003c\/p\u003e \u003cp\u003e14.2 Background and Definitions 666\u003c\/p\u003e \u003cp\u003e14.3 Indirect Method 670\u003c\/p\u003e \u003cp\u003e14.4 Direct Method 675\u003c\/p\u003e \u003cp\u003e14.5 Peak Dispersion and Tailing 681\u003c\/p\u003e \u003cp\u003e14.6 Chiral Stationary Phases and Their Characteristics 681\u003c\/p\u003e \u003cp\u003e14.7 Thermodynamic Considerations 715\u003c\/p\u003e \u003cp\u003eReferences 718\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 Preparative Separations 725 \u003cbr\u003e \u003c\/b\u003ewith \u003ci\u003eGeoff Cox\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e15.1 Introduction 726\u003c\/p\u003e \u003cp\u003e15.2 Equipment for Prep-LC Separation 730\u003c\/p\u003e \u003cp\u003e15.3 Isocratic Elution 736\u003c\/p\u003e \u003cp\u003e15.4 Severely Overloaded Separation 748\u003c\/p\u003e \u003cp\u003e15.5 Gradient Elution 751\u003c\/p\u003e \u003cp\u003e15.6 Production-Scale Separation 754\u003c\/p\u003e \u003cp\u003eReferences 755\u003c\/p\u003e \u003cp\u003e\u003cb\u003e16 Sample Preparation 757 \u003cbr\u003e \u003c\/b\u003ewith \u003ci\u003eRonald Majors\u003c\/i\u003e 757\u003c\/p\u003e \u003cp\u003e16.1 Introduction 758\u003c\/p\u003e \u003cp\u003e16.2 Types of Samples 759\u003c\/p\u003e \u003cp\u003e16.3 Preliminary Processing of Solid and Semi-Solid Samples 760\u003c\/p\u003e \u003cp\u003e16.4 Sample Preparation for Liquid Samples 764\u003c\/p\u003e \u003cp\u003e16.5 Liquid–Liquid Extraction 764\u003c\/p\u003e \u003cp\u003e16.6 Solid-Phase Extraction (SPE) 771\u003c\/p\u003e \u003cp\u003e16.7 Membrane Techniques in Sample Preparation 790\u003c\/p\u003e \u003cp\u003e16.8 Sample Preparation Methods for Solid Samples 791\u003c\/p\u003e \u003cp\u003e16.9 Column-Switching 796\u003c\/p\u003e \u003cp\u003e16.10 Sample Preparation for Biochromatography 797\u003c\/p\u003e \u003cp\u003e16.11 Sample Preparation for LC-MS 800\u003c\/p\u003e \u003cp\u003e16.12 Derivatization in HPLC 802\u003c\/p\u003e \u003cp\u003eReferences 805\u003c\/p\u003e \u003cp\u003e\u003cb\u003e17 Troubleshooting 809\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eQuick Fix 809\u003c\/p\u003e \u003cp\u003e17.1 Introduction 810\u003c\/p\u003e \u003cp\u003e17.2 Prevention of Problems 811\u003c\/p\u003e \u003cp\u003e17.3 Problem-Isolation Strategies 819\u003c\/p\u003e \u003cp\u003e17.4 Common Symptoms of HPLC Problems 821\u003c\/p\u003e \u003cp\u003e17.5 Troubleshooting Tables 865\u003c\/p\u003e \u003cp\u003eReferences 876\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendix I. Properties of HPLC Solvents 879\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eI.1 Solvent-Detector Compatibility 879\u003c\/p\u003e \u003cp\u003eI. 2 Solvent Polarity and Selectivity 882\u003c\/p\u003e \u003cp\u003eI. 3 Solvent Safety 885\u003c\/p\u003e \u003cp\u003eReferences 886\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendix II. Preparing Buffered Mobile Phases 887\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eII.1 Sequence of Operations 887\u003c\/p\u003e \u003cp\u003eII.2 Recipes for Some Commonly Used Buffers 888\u003c\/p\u003e \u003cp\u003eReference 890\u003c\/p\u003e \u003cp\u003eIndex 891\u003c\/p\u003e  \"In summary, I would strongly recommend this book for anyone working with LC and LC\/MS. It is a finely crafted introduction that adequately covers nearly every aspect of the science. The emphasis on basic principles and practical aspects ensures that it will be a useful reference for many years to come.\" (J Am Soc Mass Spectrom, 2011)  \u003cp\u003e\"This third edition is highly cross-referenced, so as to allow the reader to follow up on topics of special interest, or to clarify questions that may arise during reading. The third edition of Introduction to Modern Liquid Chromatography will continue to be the HPLC reference book for thousands of readers, either experienced workers who may wish to explore topics of his\/her choice, or find an answer to specific problems, or beginners who would like to understand and know the possibilities offered by the\u003cbr\u003e technique.\" (Chemistry Journals, 11 April 2011)\u003c\/p\u003e \u003cp\u003e\"This classic text on liquid chromatography has been thoroughly updated through the addition of information on modern instrumentation, columns, and troubleshooting. It is a valuable resource for practicing chromatographers at all levels..\" (Anal Bioanal Chem, 2011)\u003c\/p\u003e \u003cp\u003e\"In summary, I would strongly recommend this book for anyone working with LC and LC\/MS. It is a finely crafted introduction that adequately covers nearly every aspect of the science. The emphasis on basic principles and practical aspects ensures that it will be a useful reference for any years to come.\" (American Society for Mass Spectrometry, 21 Januay 2011)\u003c\/p\u003e \u003cp\u003e\"The text is illustrated with many figures and tables originating from authors' own work or taken from the literature . . . both groups of readers will find in this book plenty of information and inspiration.\" (Journal of Separation Science, 1 June 2010)\u003c\/p\u003e \u003cp\u003e\"Following their highly successful second edition (1979), which appeared more than 30 years ago, Snyder (LC Resources) and Kirkland (Advanced Materials Technology) have teamed with Dolan (LC Resources) and additional collaborators to provide an excellent update of their earlier work.\" (\u003ci\u003eCHOICE\u003c\/i\u003e, July 2010)\u003c\/p\u003e \u003cp\u003e\"It is difficult in these times to ever call a scientific book a \"bargain\", but this truly is. The wealth of information contained in these almost 1000 pages is invaluable. The book is comprehensive, eminently readable and approachable, and highly useful for both the skilled chromatographer and those new to the technique. Everyone using a liquid chromatographic instrument would benefit from owning a copy.\" (\u003ci\u003eJACS\u003c\/i\u003e, 2010)\u003c\/p\u003e \u003cp\u003e\"It's current, clearly-indexed, well-referenced, and comprehensive. The inclusion of John Dolan as author has significantly expanded the sections on troubleshooting, and additional expert contributors have enhanced the coverage of the specialist chapters.\" - Bruce Hamilton posted on the Chromatography Forum\u003c\/p\u003e    \u003cb\u003eLloyd R. Snyder, PhD\u003c\/b\u003e, is a Principal at LC Resources in Walnut Creek, California. He is the author or coauthor of several books including An Introduction to Separation Science, Introduction to Modern Liquid Chromatography, Second Edition, the bestselling Practical HPLC Method Development, Second Edition, and the comprehensive High-Performance Gradient Elution, all published by Wiley.  \u003cp\u003e\u003cb\u003eJoseph J. Kirkland, PhD,\u003c\/b\u003e is Vice President of Research and Development for Advanced Materials Technology, Inc., and coauthor of Introduction to Modern Liquid Chromatography, Second Edition, Practical HPLC Method Development, Second Edition, and Modern Size-Exclusion Liquid Chromatography, Second Edition, all published by Wiley.\u003c\/p\u003e \u003cp\u003e\u003cb\u003eJohn W. Dolan, PhD,\u003c\/b\u003e is a Principal at LC Resources. He is author of the popular \"LC Troubleshooting\" column in LCGC magazine and coauthor with Lloyd Snyder of Troubleshooting LC Systems and High-Performance Gradient Elution.\u003c\/p\u003e  \u003cb\u003eThe latest edition of the authoritative reference to HPLC\u003c\/b\u003e  \u003cp\u003eHigh-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and\/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This \u003ci\u003eThird Edition\u003c\/i\u003e, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples.\u003c\/p\u003e \u003cp\u003eThis carefully considered \u003ci\u003eThird Edition\u003c\/i\u003e maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as:\u003c\/p\u003e \u003cul\u003e \u003cli\u003e \u003cp\u003eThe basis of HPLC separation and the general effects of different experimental conditions\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eEquipment and detection\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eThe column—the \"heart\" of the HPLC system\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eReversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eComputer simulation, qualitative and quantitative analysis, and method validation and quality control\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eThe separation of large molecules, including both biological and synthetic polymers\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eChiral separations, preparative separations, and sample preparation\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eSystematic development of HPLC separations—new to this edition\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eTroubleshooting tricks, techniques, and case studies for both equipment and chromatograms\u003c\/p\u003e \u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eDesigned to fulfill the needs of the full range of HPLC users, from novices to experts, \u003ci\u003eIntroduction to Modern Liquid Chromatography\u003c\/i\u003e, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47989462892773,"sku":"NP9780470167540","price":163.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9780470167540.jpg?v=1761784199","url":"https:\/\/k12savings.com\/products\/introduction-to-modern-liquid-chromatography-isbn-9780470167540","provider":"K12savings","version":"1.0","type":"link"}