{"product_id":"insect-histology-isbn-9781444336962","title":"Insect Histology","description":"This title is a much needed update of Barbosa's self-published \u003ci\u003eManual of Basic Techniques in Insect Histology\u003c\/i\u003e. It is a laboratory manual of 'traditional' and 'modern' insect histology techniques, completely revised using cutting-edge methodology carried out today and includes new immunohistochemical techniques not previously looked at. \u003cp\u003e\u003ci\u003eInsect Histology\u003c\/i\u003e is designed as a resource for student and professional researchers, in academia and industry, who require basic information on the procedures that are essential for the histological display of the tissues of insects and related organisms.\u003c\/p\u003e Preface ix \u003cp\u003eAcknowledgements xi\u003c\/p\u003e \u003cp\u003eIntroduction xiii\u003c\/p\u003e \u003cp\u003eAbout the companion website xiv\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Problems of sclerotized chitin: Softening insect cuticle 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 Introduction 1\u003c\/p\u003e \u003cp\u003e1.2 General Methods 3\u003c\/p\u003e \u003cp\u003e1.3 Preparations of insect eggs 14\u003c\/p\u003e \u003cp\u003e1.4 Double Embedding Techniques 16\u003c\/p\u003e \u003cp\u003eReferences 19\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Fixation 21\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 Introduction 21\u003c\/p\u003e \u003cp\u003e2.2 Aldehyde based fixatives 21\u003c\/p\u003e \u003cp\u003e2.3 Protein denaturing 30\u003c\/p\u003e \u003cp\u003e2.4 Picric acid based 33\u003c\/p\u003e \u003cp\u003e2.5 Mercuric chloride based 37\u003c\/p\u003e \u003cp\u003e2.6 SEM\/TEM 40\u003c\/p\u003e \u003cp\u003e2.7 Other 46\u003c\/p\u003e \u003cp\u003eReferences 51\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Dehydrating, clearing, and embedding 54\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Dehydration 54\u003c\/p\u003e \u003cp\u003e3.2 Clearing 60\u003c\/p\u003e \u003cp\u003e3.3 Embedding General 65\u003c\/p\u003e \u003cp\u003e3.4 Embedding – Ester Wax 73\u003c\/p\u003e \u003cp\u003e3.5 Embedding – Methacrylate 74\u003c\/p\u003e \u003cp\u003eReferences 77\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Staining 79\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Single-contrast staining – Carmines 81\u003c\/p\u003e \u003cp\u003e4.2 Single contrast staining – Nuclear Stains 83\u003c\/p\u003e \u003cp\u003e4.3 Single contrast staining – General Stains 86\u003c\/p\u003e \u003cp\u003e4.4 Single contrast staining – Golgi 89\u003c\/p\u003e \u003cp\u003e4.5 Single contrast staining – Eggs 89\u003c\/p\u003e \u003cp\u003e4.6 Single contrast staining – Silver Stains 90\u003c\/p\u003e \u003cp\u003e4.7 Polychrome staining techniques – General 92\u003c\/p\u003e \u003cp\u003e4.8 Polychrome staining – Brain\/Nerve 102\u003c\/p\u003e \u003cp\u003e4.9 Polychrome staining – blood 103\u003c\/p\u003e \u003cp\u003e4.10 Single contrast procedures for chitinous material 105\u003c\/p\u003e \u003cp\u003e4.11 Polychrome staining procedures for chitinous material 106\u003c\/p\u003e \u003cp\u003e4.12 Polychrome staining for chitinous material – KOH 110\u003c\/p\u003e \u003cp\u003e4.13 Polychrome staining for chitinous material – Differential staining of Individual Organs 111\u003c\/p\u003e \u003cp\u003e4.14 Staining of specific tissues 113\u003c\/p\u003e \u003cp\u003e4.15 Two dye combinations 114\u003c\/p\u003e \u003cp\u003eReferences 117\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Immunohistochemical techniques 119\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction 119\u003c\/p\u003e \u003cp\u003e5.2 General immunostaining techniques 127\u003c\/p\u003e \u003cp\u003e5.3 Immunolabeling of samples for Transmission Electron Microscopy (TEM) 135\u003c\/p\u003e \u003cp\u003e5.4 Proliferation assays 140\u003c\/p\u003e \u003cp\u003e5.5 Methods to detect specific proteins 142\u003c\/p\u003e \u003cp\u003eReferences 144\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Use of genetic markers in insect histology 146\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction 146\u003c\/p\u003e \u003cp\u003e6.2 Inducible genetic markers 149\u003c\/p\u003e \u003cp\u003e6.3 Mosaic gene expression 156\u003c\/p\u003e \u003cp\u003e6.4 Fluorescent markers for live imaging and kinetic microscopy 165\u003c\/p\u003e \u003cp\u003eReferences 169\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Fluorescence 171\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction 171\u003c\/p\u003e \u003cp\u003eReferences 192\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Mounting 194\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction 194\u003c\/p\u003e \u003cp\u003eReferences 206\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Preparation of whole mounts 208\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction 208\u003c\/p\u003e \u003cp\u003eReferences 229\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Preparation of whole mounts for staining 231\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e10.1 Introduction 231\u003c\/p\u003e \u003cp\u003e10.2 Detection of NAPDHd 237\u003c\/p\u003e \u003cp\u003e10.3 SEM 238\u003c\/p\u003e \u003cp\u003e10.4 In situ hybridization 240\u003c\/p\u003e \u003cp\u003eReferences 244\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Preparation of genitalia, mouthparts and other body parts 246\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eReferences 256\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Preparation of chromosomes 258\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eReferences 288\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Preparation of other specific insect organs and tissues 290\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e13.1 Introduction 290\u003c\/p\u003e \u003cp\u003eReferences 323\u003c\/p\u003e \u003cp\u003eAppendix Dissecting fluids and saline solutions 325\u003c\/p\u003e \u003cp\u003eIndex 333\u003c\/p\u003e \u003cp\u003e“A very useful book to have in your library.”  (\u003ci\u003eBritish Journal of Entomology \u0026amp; Natural History\u003c\/i\u003e, 1 March 2015)\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003eDr Pedro Barbosa\u003c\/b\u003e is a Professor Emeritus at the Department of Entomology of the University of Maryland, College Park. He has been a professor at Rutgers University, the University of Massachusetts at Amherst and at the University of Maryland. Although his research focused on the ecology of insects and insect-plant interactions, he also has an interest in insect histology. Since his retirement in 2010 he has written several books on insects.\u003c\/p\u003e \u003cp\u003e\u003cb\u003eDr Deborah Berry\u003c\/b\u003e is Co-Director for the Histopathology and Tissue Shared Resource at the Lombardi Comprehensive Cancer Center, Georgetown University, which provides research pathology support for translational cancer research. She has over 20 years' experience in histology with a specialty in the histology of the fruit fly \u003ci\u003eDrosophila melanogaster\u003c\/i\u003e. \u003c\/p\u003e \u003cb\u003eDr Christina Kary\u003c\/b\u003e is a scientific editor with the journal \u003ci\u003eGenes \u0026amp; Development\u003c\/i\u003e at Cold Spring Harbor Laboratory Press, New York. Before becoming an editor, she earned a PhD with Dr Eric Baehrecke at the University of Maryland and was a Jane Coffin Childs post-doctoral fellow with Dr Susan Mango at Harvard University. \u003cp\u003eThis helpful guide is self-contained, holding all of the information you will need for the preparation of histological slides of all types of insect tissues. It explains the essential procedures for their preparation, providing step-by-step information on each phase, for example, on fixation, dehydrating, clearing, embedding, and staining.\u003c\/p\u003e \u003cp\u003eThe laboratory manual explains the cutting-edge methodology carried out today, and provides a thorough collection of both historically established and modern insect histology techniques side-by-side. It is the first book to present these various techniques appropriate to insects.\u003c\/p\u003e \u003cp\u003eKey issues covered include:\u003c\/p\u003e \u003cul\u003e \u003cli\u003ethe use of genetic markers in insect histology;\u003c\/li\u003e \u003cli\u003eimportant approaches for the preparation of tissues and\u003cbr\u003eorgans for SEM and TEM Fluorescence;\u003c\/li\u003e \u003cli\u003enew immunohistochemical techniques;\u003c\/li\u003e \u003cli\u003ehistological problems encountered\u003cbr\u003ein insect tissues such as sclerotized chitin, yolk-laden eggs, chromosomes,\u003cbr\u003egenitalia, etc., and how to deal with them.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003ePhotographs show the likely results of procedures, components of the process, and advantages of the use of particular approaches or compounds. As a comprehensive resource, this book is for students and researchers in entomology, systematics, developmental biology, insect cell biology, and morphology, wanting a clear introduction to the procedures for the histological display of insect tissues.\u003c\/p\u003e","brand":"Wiley-Blackwell","offers":[{"title":"Default Title","offer_id":47989430517989,"sku":"NP9781444336962","price":86.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9781444336962.jpg?v=1761784073","url":"https:\/\/k12savings.com\/products\/insect-histology-isbn-9781444336962","provider":"K12savings","version":"1.0","type":"link"}