{"product_id":"human-pluripotent-stem-cells-isbn-9781119394334","title":"Human Pluripotent Stem Cells","description":"\u003cp\u003e\u003cb\u003eComprehensive coverage of the entire induced pluripotent stem cell basic work flow\u003c\/b\u003e \u003c\/p\u003e \u003cp\u003ePluripotent stem cells (PSC) can divide indefinitely, self-renew, and can differentiate to functionally reconstitute almost any cell in the normal developmental pathway, given the right conditions. This comprehensive book, which was developed from a training course, covers all of the PSCs (embryonic, embryonic germ, and embryonic carcinoma) and their functions. It demonstrates the feeder-dependent and feeder-free culture of hESC and hiPSC, which will be referred to in all protocols as PSCs. It also addresses the methods commonly used to determine pluripotency, as defined by self-renewal marker expression and differentiation potential.\u003c\/p\u003e \u003cp\u003e\u003ci\u003eHuman Pluripotent Stem Cells: A Practical Guide\u003c\/i\u003e offers in-depth chapter coverage of introduction to stem cell, PSC culture, reprogramming, differentiation, PSC characterization, and more. It also includes four appendixes containing information on reagents, medias, and solutions; common antibodies; consumable and equipment; and logs and forms.\u003c\/p\u003e \u003cul\u003e \u003cli\u003eIncludes helpful tips and tricks that are normally omitted from regular research papers\u003c\/li\u003e \u003cli\u003eFeatures useful images to support the technical aspects and results visually as well as diagrammatic illustrations\u003c\/li\u003e \u003cli\u003ePresents specific sections (ie: reprogramming, differentiation) in a concise and easily digestible manner\u003c\/li\u003e \u003cli\u003eWritten by experts with extensive experience in stem cell technologies\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003ci\u003eHuman Pluripotent Stem Cells: A Practical Guide\u003c\/i\u003e is an ideal text for stem cell researchers, including principal investigators, and others in university and industry settings, and for new graduate students in PSC labs.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Introduction 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 Biosafety 1\u003c\/p\u003e \u003cp\u003e1.2 Biosafety Levels 2\u003c\/p\u003e \u003cp\u003e1.3 Aseptic Technique 2\u003c\/p\u003e \u003cp\u003e1.4 Storage 6\u003c\/p\u003e \u003cp\u003e1.5 Contamination 7\u003c\/p\u003e \u003cp\u003e1.6 Pluripotent Stem Cells 8\u003c\/p\u003e \u003cp\u003e1.7 Procedures 9\u003c\/p\u003e \u003cp\u003eReferences 16\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Pluripotent Stem Cell Culture 17\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 Introduction 17\u003c\/p\u003e \u003cp\u003e2.2 Materials 18\u003c\/p\u003e \u003cp\u003e2.3 Solutions 19\u003c\/p\u003e \u003cp\u003e2.4 Methods 21\u003c\/p\u003e \u003cp\u003eReferences 47\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Reprogramming 49\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introduction 49\u003c\/p\u003e \u003cp\u003e3.2 Materials 49\u003c\/p\u003e \u003cp\u003e3.3 Solutions 54\u003c\/p\u003e \u003cp\u003e3.4 Methods 57\u003c\/p\u003e \u003cp\u003eReferences 97\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Characterization 99\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Introduction 99\u003c\/p\u003e \u003cp\u003e4.2 Materials 101\u003c\/p\u003e \u003cp\u003e4.3 Solutions 103\u003c\/p\u003e \u003cp\u003e4.4 Methods 104\u003c\/p\u003e \u003cp\u003eReferences 128\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Differentiation 131\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction 131\u003c\/p\u003e \u003cp\u003e5.2 Materials 131\u003c\/p\u003e \u003cp\u003e5.3 Solutions 133\u003c\/p\u003e \u003cp\u003e5.4 Methods 135\u003c\/p\u003e \u003cp\u003eReferences 151\u003c\/p\u003e \u003cp\u003eIndex 153\u003c\/p\u003e   \u003cp\u003e\u003cb\u003eUma Lakshmipathy\u003c\/b\u003e, Director R\u0026amp;D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA \u003c\/p\u003e\u003cp\u003e\u003cb\u003eChad C. MacArthur\u003c\/b\u003e, Staff Scientist R\u0026amp;D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA \u003c\/p\u003e\u003cp\u003e\u003cb\u003eMahalakshmi Sridharan\u003c\/b\u003e, Scientist R\u0026amp;D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA \u003c\/p\u003e\u003cp\u003e\u003cb\u003eRene H. Quintanilla\u003c\/b\u003e, Scientist R\u0026amp;D, Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, USA     \u003c\/p\u003e\u003cp\u003e\u003cb\u003eComprehensive coverage of the entire induced pluripotent stem cell basic work flow\u003c\/b\u003e \u003c\/p\u003e\u003cp\u003ePluripotent stem cells (PSC) can divide indefinitely, self-renew, and can differentiate to functionally reconstitute almost any cell in the normal developmental pathway, given the right conditions. This comprehensive book, which was developed from a training course, covers all of the PSCs (embryonic, embryonic germ, and embryonic carcinoma) and their functions. It demonstrates the feeder-dependent and feeder-free culture of hESC and hiPSC, which will be referred to in all protocols as PSCs. It also addresses the methods commonly used to determine pluripotency, as defined by self-renewal marker expression and differentiation potential. \u003c\/p\u003e\u003cp\u003e\u003ci\u003eHuman Pluripotent Stem Cells: A Practical Guide\u003c\/i\u003e offers in-depth chapter coverage of introduction to stem cell, PSC culture, reprogramming, differentiation, PSC characterization, and more. \u003c\/p\u003e\u003cul\u003e \u003cli\u003eIncludes helpful tips and tricks that are normally omitted from regular research papers\u003c\/li\u003e \u003cli\u003eFeatures useful images to support the technical aspects and results visually as well as diagrammatic illustrations\u003c\/li\u003e \u003cli\u003ePresents specific sections (i.e., reprogramming, differentiation) in a concise and easily digestible manner\u003c\/li\u003e \u003cli\u003eWritten by experts with extensive experience in stem cell technologies\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003ci\u003eHuman Pluripotent Stem Cells: A Practical Guide\u003c\/i\u003e is an ideal text for stem cell researchers, including principal investigators, and others in university and industry settings, and for new graduate students in PSC labs.\u003c\/p\u003e","brand":"Wiley-Blackwell","offers":[{"title":"Default Title","offer_id":47989389689061,"sku":"NP9781119394334","price":111.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9781119394334.jpg?v=1761783920","url":"https:\/\/k12savings.com\/products\/human-pluripotent-stem-cells-isbn-9781119394334","provider":"K12savings","version":"1.0","type":"link"}