{"product_id":"human-embryonic-stem-cells-isbn-9780470033562","title":"Human Embryonic Stem Cells","description":"With this valuable practical guide, three members of the Harvard Stem Cell Institute have compiled and edited the definite handbook for the exciting new field of human embryonic stem cell research. The editors have gathered protocols from scientists with extensive reputation and expertise, describing and comparing currently used techniques for the culture of human stem cells and discussing the strengths and weaknesses of the different approaches.  \u003cp\u003e\u003ci\u003eHuman Embryonic Stem Cells: The Practical Handbook\u003c\/i\u003e contains the first centralised collection of methods used in human embryonic stem cell biology. The book covers the derivation of human stem cell lines, the obtaining of cells from human stem cell banks, the culturing and characterisation of the cells, and the differentiation of the cells \u003ci\u003ein vitro\u003c\/i\u003e and \u003ci\u003ein vivo\u003c\/i\u003e. Lastly, almost all of these protocols can also be used for analyzing and manipulating induced pluripotency iPS stem cells. This allows an even greater number of opportunities for those interested in purusing work in pluripotent stem cells, disease modelling, and other aspects of basic regenerative medicine research.\u003c\/p\u003e \u003cp\u003eThe novel and useful focus of this book sets it apart from other available books:\u003c\/p\u003e \u003cul\u003e \u003cli\u003eCompares and evaluates the protocols used in leading laboratories working on human embryonic stem cells\u003c\/li\u003e \u003cli\u003eCentred solely on practical protocols for human (not mouse) embryonic stem cell research\u003c\/li\u003e \u003cli\u003eIncludes extensive troubleshooting sections\u003c\/li\u003e \u003cli\u003eAddresses the different proclivities and behaviours of individual human embryonic cell lines\u003c\/li\u003e \u003cli\u003eContains techniques currently known only to a small number of specialised laboratories worldwide\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThis handbook represents an essential source of up-to-date practical information for all cell and developmental biologists working with human embryonic stem cells or wishing to enter the field. It is also essential reading for clinical researchers in areas such as diabetes, cardiovascular disease, and neurological diseases.\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePraise from the reviews:\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003e\"...a highly readable and useful book… A notable feature of the book is its air of openness and honesty… This book… will help many to navigate the uncharted waters of human embryonic stem cell biology.\"\u003c\/i\u003e BRITISH SOCIETY FOR CELL BIOLOGY\u003c\/p\u003e \u003cp\u003e\u003ci\u003e\"... the imaginative solutions in this book can inspire us to get past our most frustrating limitations.\"\u003c\/i\u003e CELL STEM CELL\u003c\/p\u003e \u003cp\u003e\u003ci\u003e\"... the richness in the details of each protocol presented will certainly encourage more scientists to begin studies of Human pluripotent stem cells...\"\u003c\/i\u003e  REGENERATIVE MEDICINE\u003c\/p\u003e \u003cp\u003e\u003ci\u003e\"In this fast-moving field, this [handbook] will help drive advances of more and more researchers.\" \u003c\/i\u003e DIFFERENTIATION\u003c\/p\u003e \u003cp\u003e\u003ci\u003e\"...a valuable resource for seasoned and novice researchers... an excellent addition to the reference collection of any medical library or research laboratory.\"\u003c\/i\u003e THE AMERICAN MEDICAL ASSOCIATION\u003c\/p\u003e  Foreword. Davor Solter  \u003cp\u003ePreface.\u003c\/p\u003e \u003cp\u003eSection 1: Obtaining and culturing human embryonic stem cells.\u003c\/p\u003e \u003cp\u003e1 Organization and good aseptic technique in the human embryonic stem cell laboratory.\u003cbr\u003e Minal J Patel, Emma L Stephenson, and Stephen L Minger\u003c\/p\u003e \u003cp\u003e2 Sourcing human embryonic stem cell lines.\u003cbr\u003e Glyn Stacey\u003c\/p\u003e \u003cp\u003e3 Human embryo culture for human embryonic stem cell derivation.\u003cbr\u003e R. Douglas Powers and Jeannine Witmyer\u003c\/p\u003e \u003cp\u003e4 Derivation of human embryonic stem cell lines.\u003cbr\u003e Stephen Sullivan, Dieter Egli, Hidenori Akutsu, Douglas A. Melton, Kevin Eggan and Chad A. Cowan\u003c\/p\u003e \u003cp\u003e5 Standard culture of human embryonic stem cells.\u003cbr\u003e Jeremy M. Crook, Rachel Horne, and Alan Colman\u003c\/p\u003e \u003cp\u003e6 Culture of human embryonic stem cells in chemically defined conditions.\u003cbr\u003e Julie Hsu Clark and Sheng Ding\u003c\/p\u003e \u003cp\u003eSection 2: Characterization of undifferentiated embryonic stem cells.\u003c\/p\u003e \u003cp\u003e7 Phenotypic analysis of human embryonic stem cells.\u003cbr\u003e Jonathan S. Draper,  Cheryle A. Séguin and Peter W. Andrews\u003c\/p\u003e \u003cp\u003e8 Genetic and epigenetic analysis of human embryonic stem cells.\u003cbr\u003e Laurie A. Boyer, Rudolf Jaenisch, and Maisam Mitalipova\u003c\/p\u003e \u003cp\u003e\u003cbr\u003e Section 3: Manipulation of human embryonic stem cells.\u003c\/p\u003e \u003cp\u003e9 \u003ci\u003eIn vivo\u003c\/i\u003e differentiation of human embryonic stem cells.\u003cbr\u003e Scott A. Noggle, Francesca M. Spagnoli, Ali H. Brivanlou\u003c\/p\u003e \u003cp\u003e10 \u003ci\u003eIn vitro\u003c\/i\u003e differentiation of human embryonic stem cells.\u003cbr\u003e A. Henry Sathananthan and Alan Trounson\u003c\/p\u003e \u003cp\u003e11 Differentiation of human embryonic stem cells into extraembryonic cell types.\u003cbr\u003e Cheryle A. Séguin and Jonathan S. Draper\u003c\/p\u003e \u003cp\u003e12 Directed differentiation of human embryonic stem cells into:\u003cbr\u003e (a) Early endoderm cells.\u003cbr\u003e Kenji Osafune, Alice E. Chen and Douglas A. Melton\u003cbr\u003e (b) Hepatic cells.\u003cbr\u003e Neta Lavon and Nissim Benvenisty\u003cbr\u003e (c) Pancreatic cells.\u003cbr\u003e Hiram Chipperfield\u003c\/p\u003e \u003cp\u003e13 Directed differentiation of human embryonic stem cells into:\u003cbr\u003e (a) Cardiomyocytes.\u003cbr\u003e Christine Mummery, Robert Passier and Chris Denning\u003cbr\u003e (b) Endothelial cells.\u003cbr\u003e Carrie Soukup, Shulamit Levenberg and Ondine Cleaver\u003cbr\u003e (c) Osteogenic cells.\u003cbr\u003e Jeffrey M. Karp, Alborz Mahdavi, Lino S. Ferreira, Ali Khademhosseini and Robert Langer\u003cbr\u003e (d) Hematopoietic \u003ci\u003ein vivo\u003c\/i\u003e repopulating cells.\u003cbr\u003e Shannon McKinney-Freeman, Thorsten M. Schlaeger and George Q. Daley\u003cbr\u003e (e) Lymphocytes.\u003cbr\u003e Petter S. Woll and Dan S. Kaufman\u003cbr\u003e (f) Myeloid cells.\u003cbr\u003e Chantal Cerdan and Mickie Bhatia\u003c\/p\u003e \u003cp\u003e14 Directed differentiation of human embryonic stem cells into:\u003cbr\u003e (a) Forebrain neurons.\u003cbr\u003e Emily A. Davis and Lawrence S.B. Goldstein\u003cbr\u003e (b) Dopaminergic neuron.\u003cbr\u003e Jan Pruszak and Ole Isacson\u003cbr\u003e (c) Spinal motor neurons.\u003cbr\u003e Bao-Yang Hu and Su-Chun Zhang\u003c\/p\u003e \u003cp\u003e15  (a) Gene targeting: knock out and knock in by homologous recombination.\u003cbr\u003e  Thomas P. Zwaka\u003cbr\u003e (b) RNA interference in human embryonic stem cells.\u003cbr\u003e M. William Lensch, Asmin Tulpule and Holm Zaehres\u003cbr\u003e (c) Generation of gene reporters using bacterial artificial chromosome recombineering. Andrew J. Washkowitz and David A. Shaywitz\u003c\/p\u003e \u003cp\u003eAfterword. Azim Surani\u003c\/p\u003e \u003cp\u003eIndex.\u003c\/p\u003e  \"A highly readable and useful book … .[It] will help many to navigate the uncharted waters of human embryonic stem cell biology.\"  (\u003ci\u003eThe British Society for Cell Biology Newsletter\u003c\/i\u003e, Autumn, 2008)  \u003cp\u003e\"[It] is a valuable resource for seasoned and novice researchers; it would make an excellent addition to the reference collection of any medical library or research laboratory.\" (\u003ci\u003eAmerican Medical Association\u003c\/i\u003e, September 2008)\u003c\/p\u003e \u003cp\u003e\"...the imaginative solutions in this book can inspire us to get past our most frustrating limitations.\" (\u003ci\u003eCell Stem Cell\u003c\/i\u003e, September 2007)\u003c\/p\u003e \u003cp\u003e\"...the richness in the details of each protocol presented will certainly encourage more scientists to begin studies of Human ES cells...\"  (\u003ci\u003eRegenerative Medicine\u003c\/i\u003e, 2007)\u003c\/p\u003e \u003cp\u003e\"In this fast-moving field, this [handbook] will help drive advances of more and more researchers.\"  (\u003ci\u003eDifferentiation\u003c\/i\u003e, 2007)\u003c\/p\u003e \u003cp\u003e\"A highly readable and useful book ... [This book] will help many to navigate the uncharted waters of human embryonic stem cell biology.\" (\u003ci\u003eBritish Society for Cell Biology,\u003c\/i\u003e Autumn, 2008\u003ci\u003e)\u003c\/i\u003e\u003c\/p\u003e  \u003cb\u003eStephen Sullivan:\u003c\/b\u003e Trinity College, Dublin, Ireland   \u003cp\u003e\u003cb\u003eKevin Eggan:\u003c\/b\u003e Harvard University, Cambridge, MA, USA\u003c\/p\u003e \u003cp\u003e\u003cb\u003eChad A. Cowan:\u003c\/b\u003e Harvard University, Cambridge, MA, USA\u003c\/p\u003e  Since their isolation in 1998, human embryonic stem (ES) cells have been used as a powerful experimental model for studying the mechanisms of stem cell self-renewal, pluripotency, and differentiation in humans. However, the protocols by which these cells are grown still differ considerably from laboratory to laboratory and standardisation is needed in this young area of research.  \u003cp\u003e\u003ci\u003eHuman Embryonic Stem Cells: The Practical Handbook\u003c\/i\u003e contains all basic methods for sourcing, deriving, culturing, and manipulating human ES cells. The editors have gathered the leading scientists in the field to describe the protocols that they have carried out and tested in their own labs.\u003c\/p\u003e \u003cp\u003e\u003cb\u003eThe book\u003c\/b\u003e\u003c\/p\u003e \u003cul type=\"square\"\u003e \u003cli\u003eContains practical information for newcomers to the field: from how to source human ES cells from international cell banks to organizing the laboratory to accommodate work with these cells.\u003c\/li\u003e \u003c\/ul\u003e \u003cul type=\"square\"\u003e \u003cli\u003eProvides protocols for routine culture as well as specialized protocols for directing differentiation into specific cell lineages.\u003c\/li\u003e \u003c\/ul\u003e \u003cul type=\"square\"\u003e \u003cli\u003eAddresses the different proclivities and behaviours of individual human ES cell lines: authors state which lines they tried their protocols on and, more importantly, which lines worked best.\u003c\/li\u003e \u003c\/ul\u003e \u003cul type=\"square\"\u003e \u003cli\u003eIncludes troubleshooting sections that address common problems and offer advice on how these can be overcome.\u003c\/li\u003e \u003c\/ul\u003e \u003cul type=\"square\"\u003e \u003cli\u003eFollows a simple, concise, and consistent structure: background material and hypothesizing are kept to a minimum to insure clarity and usability at the bench.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003ci\u003eHuman Embryonic Stem Cells: The Practical Handbook\u003c\/i\u003e is the essential source of up-to-date, practical information for all researchers and clinicians working with human embryonic stem cells.\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47989388542181,"sku":"NP9780470033562","price":283.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9780470033562.jpg?v=1761783917","url":"https:\/\/k12savings.com\/products\/human-embryonic-stem-cells-isbn-9780470033562","provider":"K12savings","version":"1.0","type":"link"}