{"product_id":"dynamics-and-kinetics-in-structural-biology-isbn-9781119696285","title":"Dynamics and Kinetics in Structural Biology","description":"\u003cb\u003eDynamics and Kinetics in Structural Biology\u003c\/b\u003e \u003cp\u003e\u003cb\u003eUnderstand the latest experimental tools in structural biology with this pioneering work\u003c\/b\u003e \u003c\/p\u003e\u003cp\u003eStructural biology seeks to understand the chemical mechanisms and functions of biological molecules, such as proteins, based on their atomic structures. Until recently, these structures have been studied only statically, using procedures which deliberately freeze atomic motion. However, freezing eliminates the rapid structural motions so essential to biological activity and function; the molecules are inactive. But with the recent development of X-ray free electron laser (XFEL) sources, efforts to conduct dynamic experiments have expanded using the principles of dynamics and kinetics to capture active biological molecules as they function. \u003c\/p\u003e\u003cp\u003e\u003ci\u003eDynamics and Kinetics in Structural Biology \u003c\/i\u003epromotes the development of these experiments and their successful application. It grounds readers in the foundational principles of dynamics and kinetics; proceeds through extended discussions of experimental procedures and data analysis techniques; and explores experimental frontiers in structural dynamics. The book will aid researchers to gather and interpret cutting-edge data on the dynamic structure of biological molecules, under conditions where they retain their biological functions. \u003c\/p\u003e\u003cp\u003e\u003ci\u003eDynamics and Kinetics in Structural Biology \u003c\/i\u003eoffers readers: \u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eAuthorship by founding figures in the field\u003c\/li\u003e \u003cli\u003eIn-depth presentation of time-resolved X-ray crystallography, solution scattering, and more\u003c\/li\u003e \u003cli\u003eA pioneering contribution to a rapidly developing field of study\u003c\/li\u003e\n\u003c\/ul\u003e \u003cp\u003e\u003ci\u003eDynamics and Kinetics in Structural Biology \u003c\/i\u003eis essential reading for graduate students, scientists, researchers and industry professionals engaged in structural studies of biological systems. Industry professionals considering dynamic studies in the development of new product lines will also benefit. \u003c\/p\u003e\u003cp\u003eList of Figures xii\u003c\/p\u003e \u003cp\u003eAcknowledgments xiv\u003c\/p\u003e \u003cp\u003eAcronyms\/Abbreviations xvi\u003c\/p\u003e \u003cp\u003eUnits xix\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Introduction: Principles of Kinetics and Dynamics 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 Structure, Function, and Mechanism 1\u003c\/p\u003e \u003cp\u003e1.2 Activity in the Crystal 3\u003c\/p\u003e \u003cp\u003e1.3 Other Structure-informing Techniques 7\u003c\/p\u003e \u003cp\u003e1.4 Dynamics, Kinetics, Movies, Pathways, and Functional Trajectories 8\u003c\/p\u003e \u003cp\u003e1.5 The Time-resolved Experiment: An Overview 12\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Physical Chemistry of Reactions 19\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 Introduction 19\u003c\/p\u003e \u003cp\u003e2.2 Thermodynamics: States and Equilibria 19\u003c\/p\u003e \u003cp\u003e2.3 Kinetics, Rates, and Rate Coefficients 25\u003c\/p\u003e \u003cp\u003e2.4 Enzyme Kinetics 28\u003c\/p\u003e \u003cp\u003e2.5 Transition State Theory and Energy Landscapes 34\u003c\/p\u003e \u003cp\u003e2.6 Trapping of Intermediates 38\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 The Experiment 43\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introduction 43\u003c\/p\u003e \u003cp\u003e3.2 Signal and Noise 43\u003c\/p\u003e \u003cp\u003e3.3 Reaction Initiation 48\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 The Sample 70\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 Crystal and Solution Samples 70\u003c\/p\u003e \u003cp\u003e4.2 Introduction of the Sample to the X-ray Beam: Injection and Fixed Targets 72\u003c\/p\u003e \u003cp\u003e4.3 Radiation Damage 76\u003c\/p\u003e \u003cp\u003e4.4 Optogenetics and Photopharmacology 82\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Time-resolved Crystallography, Solution Scattering, and Molecular Dynamics 92\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Time-resolved Crystallography 92\u003c\/p\u003e \u003cp\u003e5.2 Time-resolved X-ray Solution Scattering 104\u003c\/p\u003e \u003cp\u003e5.3 Molecular Dynamics Simulations 116\u003c\/p\u003e \u003cp\u003e6 X-ray Sources, Detectors, and Beamlines 126\u003c\/p\u003e \u003cp\u003e6.1 Introduction 126\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6.2 Sources of Synchrotron Radiation 127\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.3 X-ray Free Electron Lasers 136\u003c\/p\u003e \u003cp\u003e6.4 Detectors 141\u003c\/p\u003e \u003cp\u003e6.5 Beamlines and Experimental Stations 143\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Data Analysis and Interpretation 150\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction 150\u003c\/p\u003e \u003cp\u003e7.2 General Constraints on Analysis and Interpretation 152\u003c\/p\u003e \u003cp\u003e7.3 Difference Electron Density Maps 154\u003c\/p\u003e \u003cp\u003e7.4 Singular Value Decomposition (SVD) 160\u003c\/p\u003e \u003cp\u003e7.5 Features Commonly Found in DED Maps 167\u003c\/p\u003e \u003cp\u003e7.6 Refinement of Intermediate Structures 168\u003c\/p\u003e \u003cp\u003e7.7 Example: The Photosynthetic Reaction Center 169\u003c\/p\u003e \u003cp\u003e7.8 Making a Molecular Movie 172\u003c\/p\u003e \u003cp\u003e7.9 Does the Mechanism in the Crystal Represent the Mechanism in Solution? 173\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Other Structural Biology Techniques 178\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction 178\u003c\/p\u003e \u003cp\u003e8.2 Single-Particle Cryo-Electron Microscopy 178\u003c\/p\u003e \u003cp\u003e8.3 Energy Landscape Analysis 181\u003c\/p\u003e \u003cp\u003e8.4 X-ray Spectroscopy 193\u003c\/p\u003e \u003cp\u003e8.5 Nuclear Magnetic Resonance: Joseph Sachleben (University of Chicago) 202\u003c\/p\u003e \u003cp\u003e8.6 Hydrogen-Deuterium Exchange 213\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Looking Forward 222\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 Overview: Unraveling Function and Mechanism 222\u003c\/p\u003e \u003cp\u003e9.2 Single Particle Imaging, Energy Landscape Analysis, and Functional Trajectories 223\u003c\/p\u003e \u003cp\u003e9.3 Artificial Intelligence and Machine Learning 223\u003c\/p\u003e \u003cp\u003e9.4 Experimental Approaches 227\u003c\/p\u003e \u003cp\u003e9.5 Evolutionary Relevance of Trajectories 232\u003c\/p\u003e \u003cp\u003eReferences 233\u003c\/p\u003e \u003cp\u003eAppendix A Review of Crystallography 235\u003c\/p\u003e \u003cp\u003eIndex 246\u003c\/p\u003e  \u003cp\u003e\u003cb\u003eKeith Moffat, PhD \u003c\/b\u003eis the Louis Block Distinguished Service Professor of Biochemistry and Molecular Biology and a founding member of the Institute for Biophysical Dynamics, University of Chicago, USA. He pioneered the technique of time-resolved crystallography and has published very widely in the fields of biophysics and biochemistry. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eEaton E. Lattman, PhD \u003c\/b\u003ehas served as Professor at the Johns Hopkins University and the University at Buffalo, among other roles. He was director of BioXFEL, an NSF-sponsored center for the application of X-ray lasers to biology. He has years of experience in the fields of X-ray crystallography, structural biology, and related areas.   \u003c\/p\u003e\u003cp\u003e\u003cb\u003eUnderstand the latest experimental tools in structural biology with this pioneering work\u003c\/b\u003e \u003c\/p\u003e\u003cp\u003eStructural biology seeks to understand the chemical mechanisms and functions of biological molecules, such as proteins, based on their atomic structures. Until recently, these structures have been studied only statically, using procedures which deliberately freeze atomic motion. However, freezing eliminates the rapid structural motions so essential to biological activity and function; the molecules are inactive. But with the recent development of X-ray free electron laser (XFEL) sources, efforts to conduct dynamic experiments have expanded using the principles of dynamics and kinetics to capture active biological molecules as they function. \u003c\/p\u003e\u003cp\u003e\u003ci\u003eDynamics and Kinetics in Structural Biology \u003c\/i\u003epromotes the development of these experiments and their successful application. It grounds readers in the foundational principles of dynamics and kinetics; proceeds through extended discussions of experimental procedures and data analysis techniques; and explores experimental frontiers in structural dynamics. The book will aid researchers to gather and interpret cutting-edge data on the dynamic structure of biological molecules, under conditions where they retain their biological functions. \u003c\/p\u003e\u003cp\u003e\u003ci\u003eDynamics and Kinetics in Structural Biology \u003c\/i\u003eoffers readers: \u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eAuthorship by founding figures in the field\u003c\/li\u003e \u003cli\u003eIn-depth presentation of time-resolved X-ray crystallography, solution scattering, and more\u003c\/li\u003e \u003cli\u003eA pioneering contribution to a rapidly developing field of study\u003c\/li\u003e\n\u003c\/ul\u003e \u003cp\u003e\u003ci\u003eDynamics and Kinetics in Structural Biology \u003c\/i\u003eis essential reading for graduate students, scientists, researchers and industry professionals engaged in structural studies of biological systems. Industry professionals considering dynamic studies in the development of new product lines will also benefit.\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47989092450533,"sku":"NP9781119696285","price":160.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9781119696285.jpg?v=1761782764","url":"https:\/\/k12savings.com\/products\/dynamics-and-kinetics-in-structural-biology-isbn-9781119696285","provider":"K12savings","version":"1.0","type":"link"}