{"product_id":"circulating-tumor-cells-isbn-9781118915530","title":"Circulating Tumor Cells","description":"\u003cp\u003eIntroduces the reader to Circulating Tumor Cells (CTCs), their isolation method and analysis, and commercially available platforms \u003c\/p\u003e \u003cul\u003e \u003cli\u003ePresents the historical perspective and the overview of the field of circulating tumor cells (CTCs)\u003c\/li\u003e \u003cli\u003eDiscusses the state-of-art methods for CTC isolation, ranging from the macro- to micro-scale, from positive concentration to negative depletion, and from biological-property-enabled to physical-property-based approaches\u003c\/li\u003e \u003cli\u003eDetails commercially available CTC platforms\u003c\/li\u003e \u003cli\u003eDescribes post-isolation analysis and clinical translation\u003c\/li\u003e \u003cli\u003eProvides a glossary of scientific terms related to CTCs\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003cb\u003eList of Contributors xv\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eForeword xxi\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePreface xxv\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePART I INTRODUCTION 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Circulating Tumor Cells and Historic Perspectives 3\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJonathan W. Uhr\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e1.1 Early Studies on Cancer Dormancy Led to the Development of a Sensitive Assay for CTCs (1970–1998) 3\u003c\/p\u003e \u003cp\u003e1.2 Modern Era for Counting CTCs: 1998–2007 6\u003c\/p\u003e \u003cp\u003e1.3 Proof of Malignancy of CTCs 7\u003c\/p\u003e \u003cp\u003e1.4 New Experiments Involving CTCs 7\u003c\/p\u003e \u003cp\u003e1.5 Clinical Cancer Dormancy 8\u003c\/p\u003e \u003cp\u003e1.6 Human Epidermal Growth Factor Receptor 2 (HER2) Gene Amplification can be Acquired as Breast Cancer Progresses 10\u003c\/p\u003e \u003cp\u003e1.7 uPAR and HER2 Co-amplification 11\u003c\/p\u003e \u003cp\u003e1.8 Epithelial–Mesenchymal Transition (EMT) 12\u003c\/p\u003e \u003cp\u003e1.9 New Instruments to Capture CTCs 14\u003c\/p\u003e \u003cp\u003e1.10 Genotypic Analyses 15\u003c\/p\u003e \u003cp\u003e1.11 Conclusions 18\u003c\/p\u003e \u003cp\u003eReferences 20\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Introduction to Microfluidics 33\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eKangfu Chen and Z. Hugh Fan\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2.1 Introduction 33\u003c\/p\u003e \u003cp\u003e2.2 Scaling Law 36\u003c\/p\u003e \u003cp\u003e2.3 Device Fabrication 39\u003c\/p\u003e \u003cp\u003e2.4 Functional Components in Microfluidic Devices 43\u003c\/p\u003e \u003cp\u003e2.5 Concluding Remarks 46\u003c\/p\u003e \u003cp\u003eReferences 47\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePART II ISOLATION METHODS 51\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Ensemble-decision Aliquot Ranking (eDAR) for CTC Isolation and Analysis 53\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eMengxia Zhao, Perry G. Schiro, and Daniel T. Chiu\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3.1 Overview of eDAR 53\u003c\/p\u003e \u003cp\u003e3.2 Individual Components and Analytical Performance of eDAR 55\u003c\/p\u003e \u003cp\u003e3.3 Application and Downstream Analyses of eDAR 69\u003c\/p\u003e \u003cp\u003e3.4 Conclusion and Perspective 80\u003c\/p\u003e \u003cp\u003eReferences 81\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Sinusoidal Microchannels with High Aspect Ratios for CTC Selection and Analysis 85\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJoshua M. Jackson, Małgorzata A. Witek, and Steven A. Soper\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e4.1 Introduction 85\u003c\/p\u003e \u003cp\u003e4.2 Parallel Arrays of High-Aspect-Ratio, Sinusoidal Microchannels for CTC Selection 90\u003c\/p\u003e \u003cp\u003e4.3 Clinical Applications of Sinusoidal CTC Microchip 114\u003c\/p\u003e \u003cp\u003e4.4 Conclusion 118\u003c\/p\u003e \u003cp\u003eAcknowledgments 119\u003c\/p\u003e \u003cp\u003eReferences 119\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Cell Separation using Inertial Microfluidics 127\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eNivedita Nivedita and Ian Papautsky\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction 127\u003c\/p\u003e \u003cp\u003e5.2 Device Fabrication and System Setup 128\u003c\/p\u003e \u003cp\u003e5.3 Inertial Focusing in Microfluidics 129\u003c\/p\u003e \u003cp\u003e5.4 Cancer Cell Separation in Straight Microchannels 132\u003c\/p\u003e \u003cp\u003e5.5 Cancer Cell Separation in Spiral Microchannels 136\u003c\/p\u003e \u003cp\u003e5.6 Conclusions 142\u003c\/p\u003e \u003cp\u003eReferences 142\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Morphological Characteristics of CTCs and the Potential for Deformability-Based Separation 147\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eSimon P. Duffy and Hongshen Ma\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction 147\u003c\/p\u003e \u003cp\u003e6.2 Limitations of Antibody-based CTC Separation Methods 148\u003c\/p\u003e \u003cp\u003e6.3 Morphological and Biophysical Differences Between CTCs and Hematological Cells 149\u003c\/p\u003e \u003cp\u003e6.4 Historical and Recent Methods in CTC Separation Based on Biophysical Properties 153\u003c\/p\u003e \u003cp\u003e6.5 Microfluidic Ratchet for Deformability-Based Separation of CTCs 155\u003c\/p\u003e \u003cp\u003e6.6 Resettable Cell Trap for Deformability-based Separation of CTCs 160\u003c\/p\u003e \u003cp\u003e6.7 Summary 165\u003c\/p\u003e \u003cp\u003eReferences 166\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Microfabricated Filter Membranes for Capture and Characterization of Circulating Tumor Cells (CTCs) 173\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eZheng Ao, Richard J. Cote, Ram H. Datar, and Anthony Williams\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction 173\u003c\/p\u003e \u003cp\u003e7.2 Size-based Enrichment of Circulating Tumor Cells 174\u003c\/p\u003e \u003cp\u003e7.3 Comparison Between Size-based CTC Isolation and Affinity-based Isolation 177\u003c\/p\u003e \u003cp\u003e7.4 Characterization of CTCs Captured by Microfilters 178\u003c\/p\u003e \u003cp\u003e7.5 Conclusion 180\u003c\/p\u003e \u003cp\u003eReferences 181\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Miniaturized Nuclear Magnetic Resonance Platform for Rare Cell Detection and Profiling 183\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eSangmoo Jeong, Changwook Min, Huilin Shao, Cesar M. Castro,\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eRalph Weissleder, and Hakho Lee\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction 183\u003c\/p\u003e \u003cp\u003e8.2 μNMR Technology 184\u003c\/p\u003e \u003cp\u003e8.3 Clinical Application of μNMR for CTC Detection and Profiling 191\u003c\/p\u003e \u003cp\u003e8.4 Conclusion 196\u003c\/p\u003e \u003cp\u003eReferences 196\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Nanovelcro Cell-Affinity Assay for Detecting and Characterizing Circulating Tumor Cells 201\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eMillicent Lin, Anna Fong, Sharon Chen, Yang Zhang, Jie-fu Chen, Paulina Do, Morgan Fong, Shang-Fu Chen, Pauline Yang, An-Jou Liang, Qingyu Li, Min Song, Shuang Hou, and Hsian-Rong Tseng\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction 202\u003c\/p\u003e \u003cp\u003e9.2 Proof-of-Concept Demonstration of NanoVelcro Cell-Affinity Substrates 207\u003c\/p\u003e \u003cp\u003e9.3 First-Generation NanoVelcro Chips for CTC Enumeration 209\u003c\/p\u003e \u003cp\u003e9.4 Second-Generation NanoVelcro-LMD Technology for Single CTC Isolation 214\u003c\/p\u003e \u003cp\u003e9.5 Third-Generation Thermoresponsive NanoVelcro Chips 219\u003c\/p\u003e \u003cp\u003e9.6 Conclusions and Future Perspectives 220\u003c\/p\u003e \u003cp\u003eAcknowledgment 221\u003c\/p\u003e \u003cp\u003eReferences 221\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Acoustophoresis in Tumor Cell Enrichment 227\u003cbr\u003e \u003c\/b\u003e\u003ci\u003ePer Augustsson, Cecilia Magnusson, Hans Lilja, and Thomas Laurell\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e10.1 Introduction 227\u003c\/p\u003e \u003cp\u003e10.2 Factors Determining Acoustophoresis Cell Separation 230\u003c\/p\u003e \u003cp\u003e10.3 Acoustophoresis System for Separating Cells 234\u003c\/p\u003e \u003cp\u003e10.4 Acoustophoresis Platform for Clinical Sample Processing 239\u003c\/p\u003e \u003cp\u003e10.5 Unperturbed Cell Survival and Phenotype after Microchip Acoustophoresis 244\u003c\/p\u003e \u003cp\u003e10.6 Summary 246\u003c\/p\u003e \u003cp\u003eReferences 246\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Photoacoustic Flow Cytometry for Detection and Capture of Circulating Melanoma Cells 249\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJohn A. Viator, Benjamin S. Goldschmidt, Kiran Bhattacharyya, and Kyle Rood\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e11.1 Introduction 249\u003c\/p\u003e \u003cp\u003e11.2 Current Methods for Detection and Capture of CMCs 254\u003c\/p\u003e \u003cp\u003e11.3 Discussion 259\u003c\/p\u003e \u003cp\u003e11.4 Future Work 261\u003c\/p\u003e \u003cp\u003eReferences 262\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Selectin-Mediated Targeting of Circulating Tumor Cells for Isolation and Treatment 267\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJocelyn R. Marshall and Michael R. King\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction 267\u003c\/p\u003e \u003cp\u003e12.2 CTC Capture by E-selectin 271\u003c\/p\u003e \u003cp\u003e12.3 Applications for E-selectin in Cancer Diagnosis and Treatment 273\u003c\/p\u003e \u003cp\u003e12.4 Conclusions 278\u003c\/p\u003e \u003cp\u003eReferences 279\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Aptamer-Enabled Tumor Cell Isolation 287\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJinling Zhang and Z. Hugh Fan\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e13.1 Introduction 287\u003c\/p\u003e \u003cp\u003e13.2 Aptamers and their Biomedical Applications 288\u003c\/p\u003e \u003cp\u003e13.3 Aptamer-based Tumor Cell Isolation 290\u003c\/p\u003e \u003cp\u003e13.4 Conclusion and Outlook 297\u003c\/p\u003e \u003cp\u003eReferences 297\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 Depletion of Normal Cells for CTC Enrichment 301\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJeffrey J. Chalmers, Maryam B. Lustberg, Clayton Deighan, Kyoung-Joo Jenny Park, Yongqi Wu, and Peter Amaya\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e14.1 Introduction 301\u003c\/p\u003e \u003cp\u003e14.2 Estimates of Number and Type of Cells in Blood 302\u003c\/p\u003e \u003cp\u003e14.3 Summary of Examples of Negative Depletion 303\u003c\/p\u003e \u003cp\u003e14.4 Types of Cells Observed After Depletion of Normal Cells 305\u003c\/p\u003e \u003cp\u003e14.5 Incomplete Depletion of Normal Cells 305\u003c\/p\u003e \u003cp\u003e14.6 Conclusion 310\u003c\/p\u003e \u003cp\u003eReferences 311\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePART III POST-ISOLATION ANALYSIS AND CLINICAL TRANSLATION 313\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 Tumor Heterogeneity and Single-cell Analysis of CTCs 315\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eEvelyn K. Sigal and Stefanie S. Jeffrey\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e15.1 Introduction 315\u003c\/p\u003e \u003cp\u003e15.2 Tumor Heterogeneity 316\u003c\/p\u003e \u003cp\u003e15.3 Single-Cell Analysis of CTCs and CTC Heterogeneity 318\u003c\/p\u003e \u003cp\u003e15.4 Gene Expression Analysis 319\u003c\/p\u003e \u003cp\u003e15.5 Mutational Analysis 321\u003c\/p\u003e \u003cp\u003e15.6 Conclusion: Clinical Implications and Future Perspectives 323\u003c\/p\u003e \u003cp\u003eReferences 324\u003c\/p\u003e \u003cp\u003e\u003cb\u003e16 Single-Cell Molecular Profiles and Biophysical Assessment of Circulating Tumor Cells 329\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eDevalingam Mahalingam, Pawel Osmulski, Chiou-Miin Wang, Aaron M. Horning, Anna D. Louie, Chun-Lin Lin, Maria E. Gaczynska, and Chun-Liang Chen\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e16.1 Introduction 329\u003c\/p\u003e \u003cp\u003e16.2 Methods 331\u003c\/p\u003e \u003cp\u003e16.3 CTC Applications 336\u003c\/p\u003e \u003cp\u003e16.4 Conclusions 342\u003c\/p\u003e \u003cp\u003eReferences 343\u003c\/p\u003e \u003cp\u003e\u003cb\u003e17 Directing Circulating Tumor Cell Technologies Into Clinical Practice 351\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eBenjamin P. Casavant, David Kosoff, and Joshua M. Lang\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e17.1 Introduction 351\u003c\/p\u003e \u003cp\u003e17.2 Defining Biomarkers 352\u003c\/p\u003e \u003cp\u003e17.3 The Technology 356\u003c\/p\u003e \u003cp\u003e17.4 Translating Technology 357\u003c\/p\u003e \u003cp\u003e17.5 Conclusions 360\u003c\/p\u003e \u003cp\u003eReferences 361\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePART IV COMMERCIALIZATION 365\u003cbr\u003e 18 DEPArray™ Technology for Single CTC Analysis 367\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eFarideh Z. Bischoff, Gianni Medoro, and Nicolo Manaresi\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e18.1 Challenges in Molecular Profiling of CTCs 367\u003c\/p\u003e \u003cp\u003e18.2 DEPArray™ Technology Solution 368\u003c\/p\u003e \u003cp\u003e18.3 DEPArray™ for Single Tumor Cell Analysis 369\u003c\/p\u003e \u003cp\u003e18.4 Clinical Significance in Single CTC Profiling 373\u003c\/p\u003e \u003cp\u003e18.5 Conclusion 374\u003c\/p\u003e \u003cp\u003eReferences 374\u003c\/p\u003e \u003cp\u003e\u003cb\u003e19 CELLSEARCH® Instrument, Features, and Usage 377\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eDenis A. Smirnov, Brad W. Foulk, Mark C. Connelly, and Robert T. McCormack\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e19.1 Introduction 377\u003c\/p\u003e \u003cp\u003e19.2 Principles of CELLSEARCH® 379\u003c\/p\u003e \u003cp\u003e19.3 EpCAM Density and CTC Capture 380\u003c\/p\u003e \u003cp\u003e19.4 Clinical Applications of CELLSEARCH® CTCs 383\u003c\/p\u003e \u003cp\u003e19.5 Beyond EpCAM Capture 390\u003c\/p\u003e \u003cp\u003e19.6 Discussion 391\u003c\/p\u003e \u003cp\u003eReferences 394\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePART V GLOSSARY 401\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eCirculating Tumor Cell Glossary 403\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJose I. Varillas and Z. Hugh Fan\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eIndex 423\u003c\/b\u003e\u003c\/p\u003e \u003cb\u003eZ. Hugh Fan, PhD,\u003c\/b\u003e is a professor of the Department of Mechanical and Aerospace Engineering, J. Crayton Pruitt Family Department of Biomedical Engineering, and Department of Chemistry at the University of Florida (UF), USA. Prior to joining UF in 2003, Dr. Fan was a Principal Scientist at ACLARA BioSciences Inc. and was previously a Member of the Technical Staff at Sarnoff Corp. Dr. Fan has been recognized with E.T.S. Walton Award from Science Foundation of Ireland in 2009, Fraunhofer-Bessel Research Award from Alexander von Humboldt Foundation (Germany) in 2010, and UF Research Foundation Professorship in 2014. \u003cp\u003e\u003cb\u003ePresents a range of topics in the area of Circulating Tumor Cells including their isolation and analysis\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eCirculating Tumor Cells (CTCs) in peripheral blood play a key role in cancer metastasis because cancer cells must transport through the circulatory system before colonizing the secondary sites. CTCs have been advocated as potential biomarkers for cancer diagnosis, prognosis, and theragnosis or personalized medicine.  As a result, CTC isolation and analysis is an important topic in research, medical, and clinical communities.\u003c\/p\u003e \u003cp\u003eEarly chapters in \u003ci\u003eCirculating Tumor Cells: Isolation and Analysis\u003c\/i\u003e present an introduction to CTCs as well as historical perspective for readers both in and entering the field, while subsequent chapters explore a variety of state-of-the-art isolation methods, post-isolation analysis, clinical translation, and commercial platforms.\u003cbr\u003e\u003cbr\u003eSplit into five parts \u003ci\u003eCirculating Tumor Cells: Isolation and Analysis \u003c\/i\u003efeatures:\u003c\/p\u003e \u003cul\u003e \u003cli\u003eAn introductory chapter presenting CTCs from historic perspectives\u003c\/li\u003e \u003cli\u003eCTC isolation methods ranging from the macro- to micro-scale, from positive isolation to negative depletion, and from biological-property-enabled to physical-property-based approaches\u003c\/li\u003e \u003cli\u003ePost-isolation analysis and clinical translation, including tumor heterogeneity, single cell analysis, regulatory policy, and clinical practice\u003c\/li\u003e \u003cli\u003eCommercialization platforms such as CellSearch®, the only FDA-approved CTC platform, and DEPArrayTM, which is an instrument that can identify, quantify, and recover individual CTCs\u003c\/li\u003e \u003cli\u003eA glossary, consisting of the definition of scientific terms related to CTCs\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eBecause CTC isolation and analysis are important topics that have attracted much interest from academics, government agencies, and industry, this field has been the subject of many international symposia, calls-for-proposals from funding agencies, and articles in high-impact journals. The significance of CTCs and potential market values make companies and investors interested in the field. Many researchers, as well as students will find this book a welcome addition to gain new insight to this rapidly growing field.\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47988922188005,"sku":"NP9781118915530","price":167.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9781118915530.jpg?v=1761782063","url":"https:\/\/k12savings.com\/products\/circulating-tumor-cells-isbn-9781118915530","provider":"K12savings","version":"1.0","type":"link"}