{"product_id":"bioprocessing-technology-for-production-of-biopharmaceuticals-and-bioproducts-isbn-9781118361986","title":"Bioprocessing Technology for Production of Biopharmaceuticals and Bioproducts","description":"Written for industrial and academic researchers and development scientists in the life sciences industry, \u003ci\u003eBioprocessing Technology for Production of Biopharmaceuticals and Bioproducts\u003c\/i\u003e is a guide to the tools, approaches, and useful developments in bioprocessing. This important guide: \u003cbr\u003e\u003cbr\u003e•    Summarizes state-of-the-art bioprocessing methods and reviews applications in life science industries \u003cbr\u003e•    Includes illustrative case studies that review six milestone bio-products \u003cbr\u003e•    Discuses a wide selection of host strain types and disruptive bioprocess technologies \u003cp\u003eList of Contributors xi\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart I Case Study 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Bacillus and the Story of Protein Secretion and Production 3\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eGiulia Barbieri, Anthony Calabria, Gopal Chotani, and Eugenio Ferrari\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e1.1 Bacillus as a Production Host: Introduction and Historical Account 3\u003c\/p\u003e \u003cp\u003e1.2 The Building of a Production Strain: Genetic Tools for B. subtilis Manipulation 5\u003c\/p\u003e \u003cp\u003e1.2.1 Promoters 5\u003c\/p\u003e \u003cp\u003e1.2.2 Vectors for Building a Production Strain 6\u003c\/p\u003e \u003cp\u003e1.2.3 B. subtilis Competent Cell Transformation 7\u003c\/p\u003e \u003cp\u003e1.2.4 Protoplasts-Mediated Manipulations 9\u003c\/p\u003e \u003cp\u003e1.2.5 Genetics by Electroporation 9\u003c\/p\u003e \u003cp\u003e1.3 B. subtilis Secretion Systemand Heterologous Protein Production 9\u003c\/p\u003e \u003cp\u003e1.3.1 Bacillus Fermentation and Recovery of Industrial Enzyme 11\u003c\/p\u003e \u003cp\u003e1.3.2 Fermentation Stoichiometry 12\u003c\/p\u003e \u003cp\u003e1.3.3 Fermentor Kinetics and Outputs 14\u003c\/p\u003e \u003cp\u003e1.3.4 Downstream Processing 17\u003c\/p\u003e \u003cp\u003e1.4 Summary 21\u003c\/p\u003e \u003cp\u003eReferences 21\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 New Expression Systems for GPCRs 29\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eDimitra Gialama, Fragiskos N. Kolisis, and Georgios Skretas\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2.1 Introduction 29\u003c\/p\u003e \u003cp\u003e2.2 Recombinant GPCR Production – Traditional Approaches for Achieving High-Level Production 39\u003c\/p\u003e \u003cp\u003e2.3 Engineered Expression Systems for GPCR Production 42\u003c\/p\u003e \u003cp\u003e2.3.1 Bacteria 42\u003c\/p\u003e \u003cp\u003e2.3.2 Yeasts 48\u003c\/p\u003e \u003cp\u003e2.3.3 Insect Cells 51\u003c\/p\u003e \u003cp\u003e2.3.4 Mammalian Cells 54\u003c\/p\u003e \u003cp\u003e2.3.5 Transgenic Animals 54\u003c\/p\u003e \u003cp\u003e2.3.6 Cell-Free Systems 56\u003c\/p\u003e \u003cp\u003e2.4 Conclusion 57\u003c\/p\u003e \u003cp\u003eReferences 58\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Glycosylation 71\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eMaureen Spearman, Erika Lattová, Hélène Perreault, andMichael Butler\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introduction 71\u003c\/p\u003e \u003cp\u003e3.2 Types of Glycosylation 72\u003c\/p\u003e \u003cp\u003e3.2.1 N-linked Glycans 72\u003c\/p\u003e \u003cp\u003e3.2.2 O-linked Glycans 74\u003c\/p\u003e \u003cp\u003e3.3 Factors Affecting Glycosylation 76\u003c\/p\u003e \u003cp\u003e3.3.1 Nutrient Depletion 76\u003c\/p\u003e \u003cp\u003e3.3.2 Fed-batch Cultures and Supplements 79\u003c\/p\u003e \u003cp\u003e3.3.3 Specific Culture Supplements 80\u003c\/p\u003e \u003cp\u003e3.3.4 Ammonia 82\u003c\/p\u003e \u003cp\u003e3.3.5 pH 82\u003c\/p\u003e \u003cp\u003e3.3.6 Oxygen 83\u003c\/p\u003e \u003cp\u003e3.3.7 Host Cell Systems 83\u003c\/p\u003e \u003cp\u003e3.3.8 Other Factors 85\u003c\/p\u003e \u003cp\u003e3.4 Modification of Glycosylation 86\u003c\/p\u003e \u003cp\u003e3.4.1 siRNA and Gene Knockout\/Knockin 86\u003c\/p\u003e \u003cp\u003e3.4.2 Glycoprotein Processing Inhibitors and In Vitro Modification of Glycans 88\u003c\/p\u003e \u003cp\u003e3.5 Glycosylation Analysis 89\u003c\/p\u003e \u003cp\u003e3.5.1 Release of Glycans from Glycoproteins 90\u003c\/p\u003e \u003cp\u003e3.5.2 Derivatization of Glycans 91\u003c\/p\u003e \u003cp\u003e3.6 Methods of Analysis 91\u003c\/p\u003e \u003cp\u003e3.6.1 Lectin Arrays 91\u003c\/p\u003e \u003cp\u003e3.6.2 Liquid Chromatography 93\u003c\/p\u003e \u003cp\u003e3.6.2.1 HILIC Analysis 93\u003c\/p\u003e \u003cp\u003e3.6.2.2 Reversed Phase (RP) and Porous Graphitic Carbon (PGC) Chromatography 95\u003c\/p\u003e \u003cp\u003e3.6.2.3 Weak Anion Exchange (WAX) HPLC Analysis 96\u003c\/p\u003e \u003cp\u003e3.6.2.4 High pH Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD) 96\u003c\/p\u003e \u003cp\u003e3.6.3 Capillary Electrophoresis (CE) 97\u003c\/p\u003e \u003cp\u003e3.6.4 Fluorophore-assisted Carbohydrate Electrophoresis (FACE) and CGE-LIF 99\u003c\/p\u003e \u003cp\u003e3.6.5 Mass Spectrometry (MS) 100\u003c\/p\u003e \u003cp\u003e3.6.5.1 Ionization 100\u003c\/p\u003e \u003cp\u003e3.6.5.2 Derivatization Techniques Used for MS Analysis of Glycans 102\u003c\/p\u003e \u003cp\u003e3.6.5.3 Fragmentation of Carbohydrates 103\u003c\/p\u003e \u003cp\u003e3.7 Conclusion 109\u003c\/p\u003e \u003cp\u003eReferences 109\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart II Bioreactors 131\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Bioreactors for StemCell and Mammalian Cell Cultivation 133\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eAna Fernandes-Platzgummer, Sara M. Badenes, Cláudia L. da Silva, and JoaquimM. S. Cabral\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e4.1 Overview of (Mammalian and Stem) Cell Culture Engineering 133\u003c\/p\u003e \u003cp\u003e4.1.1 Cell Products for Therapeutics 134\u003c\/p\u003e \u003cp\u003e4.1.2 Cell as a Product: Stem Cells 136\u003c\/p\u003e \u003cp\u003e4.2 Bioprocess Characterization 140\u003c\/p\u003e \u003cp\u003e4.2.1 Cell Cultivation Methods 140\u003c\/p\u003e \u003cp\u003e4.2.2 Cell Metabolism 141\u003c\/p\u003e \u003cp\u003e4.2.3 Culture Medium Design 143\u003c\/p\u003e \u003cp\u003e4.2.4 Culture Parameters 144\u003c\/p\u003e \u003cp\u003e4.2.5 Culture Modes 145\u003c\/p\u003e \u003cp\u003e4.3 Cell Culture Systems 147\u003c\/p\u003e \u003cp\u003e4.3.1 Static Culture Systems 147\u003c\/p\u003e \u003cp\u003e4.3.2 Roller Bottles 150\u003c\/p\u003e \u003cp\u003e4.3.3 Spinner Flask 150\u003c\/p\u003e \u003cp\u003e4.3.4 Airlift Bioreactor 151\u003c\/p\u003e \u003cp\u003e4.3.5 Fixed\/Fluidized-Bed Bioreactor 152\u003c\/p\u003e \u003cp\u003e4.3.6 Wave Bioreactor 152\u003c\/p\u003e \u003cp\u003e4.3.7 Rotating-Wall Vessel Bioreactor 154\u003c\/p\u003e \u003cp\u003e4.3.8 Stirred Tank Bioreactor 155\u003c\/p\u003e \u003cp\u003e4.3.8.1 Agitation\/Shear Stress 156\u003c\/p\u003e \u003cp\u003e4.4 Cell Culture Modeling 157\u003c\/p\u003e \u003cp\u003e4.5 Case Studies 159\u003c\/p\u003e \u003cp\u003e4.5.1 Antibody Production in Bioreactor Systems 159\u003c\/p\u003e \u003cp\u003e4.5.2 mESC Expansion on Microcarriers in a Stirred Tank Bioreactor 161\u003c\/p\u003e \u003cp\u003e4.6 Concluding Remarks 162\u003c\/p\u003e \u003cp\u003eList of Symbols 163\u003c\/p\u003e \u003cp\u003eReferences 164\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Model-Based Technologies Enabling Optimal Bioreactor Performance 175\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eRimvydas Simutis, Marco Jenzsch, and Andreas Lübbert\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction 175\u003c\/p\u003e \u003cp\u003e5.2 Basics 176\u003c\/p\u003e \u003cp\u003e5.2.1 Balances 176\u003c\/p\u003e \u003cp\u003e5.2.2 Model Identification 177\u003c\/p\u003e \u003cp\u003e5.2.3 Model-Based Process Optimization 178\u003c\/p\u003e \u003cp\u003e5.3 Examples 180\u003c\/p\u003e \u003cp\u003e5.3.1 Model-Based State Estimation 180\u003c\/p\u003e \u003cp\u003e5.3.1.1 Static Model Approach 180\u003c\/p\u003e \u003cp\u003e5.3.1.2 Dynamic Alternatives 183\u003c\/p\u003e \u003cp\u003e5.3.2 Optimizing Open Loop-Controlled Cultivations 184\u003c\/p\u003e \u003cp\u003e5.3.2.1 Robust Cultivation Profiles 184\u003c\/p\u003e \u003cp\u003e5.3.2.2 Evolutionary Modeling Approach 188\u003c\/p\u003e \u003cp\u003e5.3.3 Optimization by Model-Aided Feedback Control 190\u003c\/p\u003e \u003cp\u003e5.3.3.1 Improving the Basic Control 190\u003c\/p\u003e \u003cp\u003e5.3.3.2 Optimizing the Amount of Soluble Product 190\u003c\/p\u003e \u003cp\u003e5.3.4 CO2-Removal in Large-Scale Cell Cultures 194\u003c\/p\u003e \u003cp\u003e5.4 Conclusion 197\u003c\/p\u003e \u003cp\u003eReferences 198\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Monitoring and Control of Bioreactor: Basic Concepts and Recent Advances 201\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eJames Gomes, Viki Chopda, and Anurag S. Rathore\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction 201\u003c\/p\u003e \u003cp\u003e6.2 Challenges in Bioprocess Control 202\u003c\/p\u003e \u003cp\u003e6.2.1 Process Dynamics and Modeling 202\u003c\/p\u003e \u003cp\u003e6.2.2 Limits of Hardware and Software andTheir Integration 203\u003c\/p\u003e \u003cp\u003e6.2.3 Regulatory Aspects 204\u003c\/p\u003e \u003cp\u003e6.3 Basic Elements of Bioprocess Control 205\u003c\/p\u003e \u003cp\u003e6.3.1 Bioprocess Monitoring 205\u003c\/p\u003e \u003cp\u003e6.3.2 Parameter Estimators 205\u003c\/p\u003e \u003cp\u003e6.3.3 Bioprocess Modeling 206\u003c\/p\u003e \u003cp\u003e6.4 Current Practices in Bioprocess Control 208\u003c\/p\u003e \u003cp\u003e6.4.1 PID Control 208\u003c\/p\u003e \u003cp\u003e6.4.2 Model-Based Control 209\u003c\/p\u003e \u003cp\u003e6.4.3 Adaptive Control 211\u003c\/p\u003e \u003cp\u003e6.4.4 Nonlinear Control 214\u003c\/p\u003e \u003cp\u003e6.5 Intelligent Control Systems 217\u003c\/p\u003e \u003cp\u003e6.5.1 Fuzzy Control 217\u003c\/p\u003e \u003cp\u003e6.5.2 Neural Control 219\u003c\/p\u003e \u003cp\u003e6.5.3 Statistical Process Control 222\u003c\/p\u003e \u003cp\u003e6.5.4 Integrated and Plant-Wide Bioprocess Control 224\u003c\/p\u003e \u003cp\u003e6.5.5 Metabolic Control 225\u003c\/p\u003e \u003cp\u003e6.6 Summary 226\u003c\/p\u003e \u003cp\u003e6.7 Future Perspectives 227\u003c\/p\u003e \u003cp\u003eAcknowledgments 227\u003c\/p\u003e \u003cp\u003eReferences 227\u003c\/p\u003e \u003cp\u003ePart III Host Strain Technologies 239\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Metabolic Engineering for Biocatalyst Robustness to Organic Inhibitors 241\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eLiam Royce and Laura R. Jarboe\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction 241\u003c\/p\u003e \u003cp\u003e7.2 Mechanisms of Inhibition 243\u003c\/p\u003e \u003cp\u003e7.3 Mechanisms of Tolerance 245\u003c\/p\u003e \u003cp\u003e7.4 Membrane Engineering 246\u003c\/p\u003e \u003cp\u003e7.5 Evolutionary and Metagenomic Strategies for Increasing Tolerance 251\u003c\/p\u003e \u003cp\u003e7.6 Reverse Engineering of Improved Strains 254\u003c\/p\u003e \u003cp\u003e7.7 Concluding Remarks 255\u003c\/p\u003e \u003cp\u003eAcknowledgments 255\u003c\/p\u003e \u003cp\u003eReferences 255\u003c\/p\u003e \u003cp\u003eIndex 267\u003c\/p\u003e  \u003cp\u003e\u003cb\u003eCLAIRE KOMIVES, P\u003csmall\u003eH\u003c\/small\u003eD,\u003c\/b\u003e is a Professor in the Chemical and Materials Engineering Department at San Jose State University. Her research interests focus on the development of low cost snake antivenom compounds. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eWEICHANG ZHOU, P\u003csmall\u003eH\u003c\/small\u003eD,\u003c\/b\u003e is Chief Technology Officer, Biologics Development \u0026amp; Manufacturing at WuXi Biologics. He has previously worked at Genzyme Corporation and Merck Research Laboratories.   \u003c\/p\u003e\u003cp\u003e\u003cb\u003eA Guide to the Latest Developments in Bioprocessing that can be Applied in both the Biopharmaceutical and Biochemical Industries\u003c\/b\u003e \u003c\/p\u003e\u003cp\u003e\u003ci\u003eBioprocessing Technology for Production of Biopharmaceuticals and Bioproducts\u003c\/i\u003e offers a review of some current developments in bioprocessing and puts an emphasis on the effective tools and methods. With contributions from noted experts in the field, the text contains a review of six enabling technologies that cover a broad range of scientific and engineering topics. The authors include information about the history of the process and technologies used in the development of a selection of biopharmaceuticals and bioproducts. \u003c\/p\u003e\u003cp\u003eAs the authors explain, the development of new processing methods and the improvement of traditional reactors and host strains has led to recent advances in manufacturing sciences. Given these new developments, the book explores the engineering methods that can be used for large-scale applications. \u003ci\u003eBioprocessing Technology for Production of Biopharmaceuticals and Bioproducts\u003c\/i\u003e contains the information needed to better understand both the reactor technology as well as the characteristics and specific needs of the host strains. Host strains include primarily mammalian cells, and bacteria. This important guide: \u003c\/p\u003e\u003cul\u003e \u003cli\u003eContains a concise summary of the state-of-the-art bioprocessing methods\u003c\/li\u003e \u003cli\u003eReviews the broad spectrum of applications to a variety of life science industries\u003c\/li\u003e \u003cli\u003eIncludes illustrative case studies that review several milestone bio-products\u003c\/li\u003e \u003cli\u003eExplores of a wide selection of host strain types\u003c\/li\u003e \u003cli\u003eContains information on disruptive bioprocess technologies\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eWritten for industrial and academic researchers and development scientists in the life sciences industry, \u003ci\u003eBioprocessing Technology for Production of Biopharmaceuticals and Bioproducts\u003c\/i\u003e is a guide to the tools, approaches, and useful developments in bioprocessing.\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47988841119973,"sku":"NP9781118361986","price":154.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9781118361986.jpg?v=1761781732","url":"https:\/\/k12savings.com\/products\/bioprocessing-technology-for-production-of-biopharmaceuticals-and-bioproducts-isbn-9781118361986","provider":"K12savings","version":"1.0","type":"link"}