{"product_id":"biomolecular-and-bioanalytical-techniques-isbn-9781119483960","title":"Biomolecular and Bioanalytical Techniques","description":"\u003cp\u003e\u003cb\u003eAn essential guide to biomolecular and bioanalytical techniques and their applications\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eBiomolecular and Bioanalytical Techniques\u003c\/i\u003e offers an introduction to, and a basic understanding of, a wide range of biophysical techniques. The text takes an interdisciplinary approach with contributions from a panel of distinguished experts. With a focus on research, the text comprehensively covers a broad selection of topics drawn from contemporary research in the fields of chemistry and biology. Each of the internationally reputed authors has contributed a single chapter on a specific technique. The chapters cover the specific technique’s background, theory, principles, technique, methodology, protocol and applications. \u003c\/p\u003e \u003cp\u003eThe text explores the use of a variety of analytical tools to characterise biological samples. The contributors explain how to identify and quantify biochemically important molecules, including small molecules as well as biological macromolecules such as enzymes, antibodies, proteins, peptides and nucleic acids. This book is filled with essential knowledge and explores the skills needed to carry out the research and development roles in academic and industrial laboratories.\u003c\/p\u003e \u003cul\u003e \u003cli\u003eA technique-focused book that bridges the gap between an introductory text and a book on advanced research methods\u003c\/li\u003e \u003cli\u003eProvides the necessary background and skills needed to advance the research methods\u003c\/li\u003e \u003cli\u003eFeatures a structured approach within each chapter\u003c\/li\u003e \u003cli\u003eDemonstrates an interdisciplinary approach that serves to develop independent thinking\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eWritten for students in chemistry, biological, medical, pharmaceutical, forensic and biophysical sciences, \u003ci\u003eBiomolecular and Bioanalytical Techniques\u003c\/i\u003e is an in-depth review of the most current biomolecular and bioanalytical techniques in the field. \u003c\/p\u003e \u003cp\u003eList of Contributors xix\u003c\/p\u003e \u003cp\u003ePreface xxiii\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Principles of Health and Safety and Good Laboratory Practice \u003c\/b\u003e\u003cb\u003e1\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eElaine Armstrong\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e1.1 Introduction 1\u003c\/p\u003e \u003cp\u003e1.2 Good Laboratory Practice 1\u003c\/p\u003e \u003cp\u003e1.3 Risk Assessment 2\u003c\/p\u003e \u003cp\u003e1.4 Chemical Risk Assessment 5\u003c\/p\u003e \u003cp\u003e1.5 Biological Materials and Genetically Modified Organisms 7\u003c\/p\u003e \u003cp\u003e1.6 Vacuum Apparatus, Pressure Systems and Associated Glassware 9\u003c\/p\u003e \u003cp\u003e1.7 Cryogenic Liquefied Gases 9\u003c\/p\u003e \u003cp\u003e1.8 Compressed Gas Cylinders 10\u003c\/p\u003e \u003cp\u003e1.9 Electromagnetic Radiation 10\u003c\/p\u003e \u003cp\u003e1.10 Lasers 11\u003c\/p\u003e \u003cp\u003e1.11 High Magnetic Fields 11\u003c\/p\u003e \u003cp\u003e1.12 Sharps 12\u003c\/p\u003e \u003cp\u003e1.13 Ergonomic Issues 12\u003c\/p\u003e \u003cp\u003eReferences 15\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Applications of Chemoinformatics in Drug Discovery \u003c\/b\u003e\u003cb\u003e17\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eValerie J. Gillet\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2.1 Significance and Background 17\u003c\/p\u003e \u003cp\u003e2.2 Computer Representation of Chemical Structures 17\u003c\/p\u003e \u003cp\u003e2.3 Database Searching 18\u003c\/p\u003e \u003cp\u003e2.4 Practical Issues on Representation 22\u003c\/p\u003e \u003cp\u003e2.5 Virtual Screening 22\u003c\/p\u003e \u003cp\u003e2.6 Ligand-Based Virtual Screening 23\u003c\/p\u003e \u003cp\u003e2.7 Protein–Ligand Docking 30\u003c\/p\u003e \u003cp\u003e2.8 Evaluating Virtual Screening Methods 32\u003c\/p\u003e \u003cp\u003e2.9 Case Studies of Virtual Screening 33\u003c\/p\u003e \u003cp\u003e2.10 Conclusions 34\u003c\/p\u003e \u003cp\u003eReferences 34\u003c\/p\u003e \u003cp\u003eFurther Reading 36\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Bioinformatics and Its Applications in Genomics \u003c\/b\u003e\u003cb\u003e37\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eDavid J. Parry-Smith\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3.1 Significance and Short Background 37\u003c\/p\u003e \u003cp\u003e3.2 Theory\/Principles 40\u003c\/p\u003e \u003cp\u003e3.3 Databases 43\u003c\/p\u003e \u003cp\u003e3.4 Techniques 45\u003c\/p\u003e \u003cp\u003e3.5 Applications 53\u003c\/p\u003e \u003cp\u003e3.6 Concluding Remarks 56\u003c\/p\u003e \u003cp\u003eReferences 56\u003c\/p\u003e \u003cp\u003eFurther Reading 57\u003c\/p\u003e \u003cp\u003eWebsites 57\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Gene Cloning for the Analysis of Gene Expression \u003c\/b\u003e\u003cb\u003e59\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eHuw B. Thomas and Raymond T. O’Keefe\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e4.1 Identifying Target Sequence 59\u003c\/p\u003e \u003cp\u003e4.2 \u003ci\u003eIn Silico\u003c\/i\u003e Design 59\u003c\/p\u003e \u003cp\u003e4.3 Primer Design 60\u003c\/p\u003e \u003cp\u003e4.4 Template Preparation 62\u003c\/p\u003e \u003cp\u003e4.5 Cloning Methods 64\u003c\/p\u003e \u003cp\u003e4.6 Uses for Cloned DNA Sequences 66\u003c\/p\u003e \u003cp\u003e4.7 Verifying Cloned Sequences 67\u003c\/p\u003e \u003cp\u003e4.8 Applications of Gene Constructs 68\u003c\/p\u003e \u003cp\u003e4.9 Case Study: Cloning of a Human Missense Variant Exon into a Minigene Splicing Vector 69\u003c\/p\u003e \u003cp\u003e4.10 Case Study: Epitope Tagging of a Yeast Gene 74\u003c\/p\u003e \u003cp\u003eReferences 79\u003c\/p\u003e \u003cp\u003eFurther Reading 79\u003c\/p\u003e \u003cp\u003eWebsites 80\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Proteomic Techniques and Their Applications \u003c\/b\u003e\u003cb\u003e81\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eHsueh-Fen Juan\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5.1 Significance and Background 81\u003c\/p\u003e \u003cp\u003e5.2 Principles of Major Proteomics Techniques 82\u003c\/p\u003e \u003cp\u003e5.3 Methods for Proteomics 85\u003c\/p\u003e \u003cp\u003e5.4 Applications 92\u003c\/p\u003e \u003cp\u003e5.5 Concluding Remarks 95\u003c\/p\u003e \u003cp\u003eAcknowledgements 95\u003c\/p\u003e \u003cp\u003eReferences 95\u003c\/p\u003e \u003cp\u003eFurther Reading 99\u003c\/p\u003e \u003cp\u003eWebsite Resources 99\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Overproduction, Separation and Purification of Affinity-Tagged Proteins from Escherichia coli \u003c\/b\u003e\u003cb\u003e101\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eFinbarr Hayes and Daniela Barillà\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction 101\u003c\/p\u003e \u003cp\u003e6.2 Selecting an Affinity Tag: Glutathione-S-Transferase, Maltose-Binding Protein and Hexa-Histidine Motifs 103\u003c\/p\u003e \u003cp\u003e6.3 The pET Vector Series: Archetypal Expression Vectors in \u003ci\u003eE. coli\u003c\/i\u003e 105\u003c\/p\u003e \u003cp\u003e6.4 IMAC of a His\u003csub\u003e6\u003c\/sub\u003e-Tagged Protein: Example Methodology with the ParF DNA Segregation Protein 107\u003c\/p\u003e \u003cp\u003e6.5 Production and Purification of a GST-Tagged Protein: Example Methodology with the C-Terminal Domain of Yeast RNA Polymerase II 112\u003c\/p\u003e \u003cp\u003e6.6 Further Purification of Tagged Proteins 114\u003c\/p\u003e \u003cp\u003e6.7 Alternative Hosts for Protein Production 116\u003c\/p\u003e \u003cp\u003e6.8 Concluding Remarks 116\u003c\/p\u003e \u003cp\u003eAcknowledgements 117\u003c\/p\u003e \u003cp\u003eReferences 117\u003c\/p\u003e \u003cp\u003eFurther Reading 121\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Chromatography: Separation Techniques in Biology 123\u003c\/b\u003e\u003cbr\u003e\u003ci\u003eW John Lough and Mark Carlile\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction to Chromatographic Separation 123\u003c\/p\u003e \u003cp\u003e7.2 General Considerations for Protein Separation by Chromatography 132\u003c\/p\u003e \u003cp\u003e7.3 Engineering Proteins for Streamlined Chromatographic Separations 142\u003c\/p\u003e \u003cp\u003e7.4 Example Chromatographic Separations of Biological Samples 143\u003c\/p\u003e \u003cp\u003e7.5 Other Applications of Chromatography for Biological Sample Preparation and Analysis 147\u003c\/p\u003e \u003cp\u003eReferences 147\u003c\/p\u003e \u003cp\u003eFurther Reading 152\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Synthetic Methodology in Chemical Biology \u003c\/b\u003e\u003cb\u003e153\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eRichard C. Brewster and Stephen Wallace\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction 153\u003c\/p\u003e \u003cp\u003e8.2 Peptide Synthesis 153\u003c\/p\u003e \u003cp\u003e8.3 Amide Bond Synthesis 154\u003c\/p\u003e \u003cp\u003e8.4 Bioorthogonal Chemistry 164\u003c\/p\u003e \u003cp\u003e8.5 The Copper-Catalysed Azide-Alkyne Cycloaddition Reaction (CuAAC) 165\u003c\/p\u003e \u003cp\u003e8.6 Unnatural Amino Acid Incorporation 169\u003c\/p\u003e \u003cp\u003e8.7 Case Studies 172\u003c\/p\u003e \u003cp\u003e8.8 Conclusion 176\u003c\/p\u003e \u003cp\u003eReferences 177\u003c\/p\u003e \u003cp\u003eFurther Reading 178\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Reaction Chemical Kinetics in Biology 179\u003c\/b\u003e\u003cbr\u003e\u003ci\u003eNicholas J. Harmer and Mirella Vivoli Vega\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e9.1 Significance 179\u003c\/p\u003e \u003cp\u003e9.2 Overview of Kinetics and Its Application to Biology 180\u003c\/p\u003e \u003cp\u003e9.3 Determination of Enzyme Kinetic Mechanisms 188\u003c\/p\u003e \u003cp\u003e9.4 Technique\/Protocol: Determination of Michaelis–Menten Parameters for a Bisubstrate Enzyme and Use of Product Inhibition to Determine Mechanism 201\u003c\/p\u003e \u003cp\u003e9.5 Case Study: Determination of Michaelis–Menten Parameters for a Bisubstrate Enzyme 210\u003c\/p\u003e \u003cp\u003e9.6 More Advanced Methods 212\u003c\/p\u003e \u003cp\u003e9.7 Concluding Remarks 214\u003c\/p\u003e \u003cp\u003eReferences 214\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Mass Spectrometry and Its Applications \u003c\/b\u003e\u003cb\u003e219\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eBlagojce Jovcevski and Tara L. Pukala\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e10.1 Significance 219\u003c\/p\u003e \u003cp\u003e10.2 Theories and Principles of Biomolecular Mass Spectrometry 221\u003c\/p\u003e \u003cp\u003e10.3 Techniques and Methodology in Biomolecular Mass Spectrometry 234\u003c\/p\u003e \u003cp\u003e10.4 Applications 242\u003c\/p\u003e \u003cp\u003e10.5 Concluding Remarks 248\u003c\/p\u003e \u003cp\u003eAbbreviations 248\u003c\/p\u003e \u003cp\u003eReferences 249\u003c\/p\u003e \u003cp\u003eFurther Reading 253\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Applications and Complementarity of Analytical Ultracentrifugation and Light-Scattering Techniques \u003c\/b\u003e\u003cb\u003e255\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eChad A. Brautigam\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e11.1 Introduction 255\u003c\/p\u003e \u003cp\u003e11.2 Analytical Ultracentrifugation 255\u003c\/p\u003e \u003cp\u003e11.3 Light Scattering 262\u003c\/p\u003e \u003cp\u003e11.4 Protocols 266\u003c\/p\u003e \u003cp\u003e11.5 Applications 270\u003c\/p\u003e \u003cp\u003e11.6 Conclusions 275\u003c\/p\u003e \u003cp\u003eAcknowledgements 275\u003c\/p\u003e \u003cp\u003eReferences 276\u003c\/p\u003e \u003cp\u003eFurther Reading 278\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Application of Isothermal Titration Calorimetry (ITC) to Biomolecular Interactions \u003c\/b\u003e\u003cb\u003e279\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eGraeme L. Conn\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction 279\u003c\/p\u003e \u003cp\u003e12.2 Principles and Theory of ITC 281\u003c\/p\u003e \u003cp\u003e12.3 Protocols for Design, Implementation and Analysis of ITC Experiments 285\u003c\/p\u003e \u003cp\u003e12.4 Example Applications of ITC to Analysis of Biomolecular Interactions 299\u003c\/p\u003e \u003cp\u003e12.5 Concluding Remarks 304\u003c\/p\u003e \u003cp\u003eAcknowledgements 304\u003c\/p\u003e \u003cp\u003eReferences 304\u003c\/p\u003e \u003cp\u003eFurther Reading 305\u003c\/p\u003e \u003cp\u003eWebsite Resources 305\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 An Introduction to Infra-red and Raman Spectroscopies for Pharmaceutical and Biomedical Studies \u003c\/b\u003e\u003cb\u003e307\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eKa Lung Andrew Chan\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e13.1 Significance and Short Background 307\u003c\/p\u003e \u003cp\u003e13.2 Theory 307\u003c\/p\u003e \u003cp\u003e13.3 Technique\/Methodology\/Protocol 313\u003c\/p\u003e \u003cp\u003e13.4 Applications 323\u003c\/p\u003e \u003cp\u003e13.5 Concluding Remarks 327\u003c\/p\u003e \u003cp\u003eReferences 327\u003c\/p\u003e \u003cp\u003eFurther Reading 331\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 Fluorescence Spectroscopy and Its Applications in Analysing Biomolecular Processes \u003c\/b\u003e\u003cb\u003e333\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eNathan N. Alder\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e14.1 Significance and Background 333\u003c\/p\u003e \u003cp\u003e14.2 Theory and Principles 334\u003c\/p\u003e \u003cp\u003e14.3 Techniques, Methodologies and Protocols 341\u003c\/p\u003e \u003cp\u003e14.4 Case Studies: Fluorescence Spectroscopy to Analyse Membrane Protein Structural Dynamics 358\u003c\/p\u003e \u003cp\u003e14.5 Concluding Remarks 360\u003c\/p\u003e \u003cp\u003eAcknowledgements 361\u003c\/p\u003e \u003cp\u003eReferences 361\u003c\/p\u003e \u003cp\u003eFurther Reading 363\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 Circular Dichroism and Related Spectroscopic Techniques \u003c\/b\u003e\u003cb\u003e365\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eSophia C. Goodchild, Krishanthi Jayasundera and Alison Rodger\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e15.1 Significance and Background 365\u003c\/p\u003e \u003cp\u003e15.2 Theory\/Principles 366\u003c\/p\u003e \u003cp\u003e15.3 Technique\/Methodology\/Protocol 371\u003c\/p\u003e \u003cp\u003e15.4 Applications 376\u003c\/p\u003e \u003cp\u003e15.5 Concluding Remarks 382\u003c\/p\u003e \u003cp\u003eReferences 382\u003c\/p\u003e \u003cp\u003eFurther Reading 384\u003c\/p\u003e \u003cp\u003e\u003cb\u003e16 Principles and Practice in Macromolecular X-Ray Crystallography \u003c\/b\u003e385\u003cbr\u003e\u003ci\u003eArnaud Baslé and Richard J. Lewis\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e16.1 Significance and Short Background 385\u003c\/p\u003e \u003cp\u003e16.2 Theory and Principles: Overview 385\u003c\/p\u003e \u003cp\u003e16.3 Methodology 397\u003c\/p\u003e \u003cp\u003e16.4 Applications 412\u003c\/p\u003e \u003cp\u003e16.5 Concluding Remarks 414\u003c\/p\u003e \u003cp\u003eAcknowledgements 414\u003c\/p\u003e \u003cp\u003eReferences 414\u003c\/p\u003e \u003cp\u003eFurther Reading 418\u003c\/p\u003e \u003cp\u003e\u003cb\u003e17 Biomolecular NMR Spectroscopy and Structure Determination of DNA \u003c\/b\u003e\u003cb\u003e421\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eTony Cheung and Vasudevan Ramesh\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e17.1 Significance and Background 421\u003c\/p\u003e \u003cp\u003e17.2 Basic NMR Theory 422\u003c\/p\u003e \u003cp\u003e17.3 Multidimensional NMR Spectroscopy 428\u003c\/p\u003e \u003cp\u003e17.4 NMR Instrumentation and Experiments 429\u003c\/p\u003e \u003cp\u003e17.5 Structure and Conformational Parameters of DNA 435\u003c\/p\u003e \u003cp\u003e17.6 NMR Structure Determination 440\u003c\/p\u003e \u003cp\u003e17.7 Case Study 1: NMR Structure Determination and Conformational Analysis of 17mer Canonical GC DNA 446\u003c\/p\u003e \u003cp\u003e17.8 Case Study 2: NMR Structure Determination and Conformational Analysis of 13mer 6-ThioguanineModified GC DNA 460\u003c\/p\u003e \u003cp\u003e17.9 Conclusion 464\u003c\/p\u003e \u003cp\u003eReferences 466\u003c\/p\u003e \u003cp\u003eFurther Reading 469\u003c\/p\u003e \u003cp\u003e\u003cb\u003e18 Cryo-TEM and Biological Structure Determination \u003c\/b\u003e\u003cb\u003e471\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eSzymonW. Manka and Carolyn A. Moores\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e18.1 Significance and Background 471\u003c\/p\u003e \u003cp\u003e18.2 Theoretical Principles of Biological Cryo-TEM 473\u003c\/p\u003e \u003cp\u003e18.3 Experimental Approaches in Biological Cryo-TEM 481\u003c\/p\u003e \u003cp\u003e18.4 Cryo-TEM Case Studies 491\u003c\/p\u003e \u003cp\u003e18.5 Concluding Remarks 496\u003c\/p\u003e \u003cp\u003eAcknowledgements 496\u003c\/p\u003e \u003cp\u003eReferences 496\u003c\/p\u003e \u003cp\u003eWebsite Resources 499\u003c\/p\u003e \u003cp\u003e\u003cb\u003e19 Computer Modelling and Molecular Dynamics Simulation of Biomolecules \u003c\/b\u003e\u003cb\u003e501\u003cbr\u003e\u003c\/b\u003e\u003ci\u003eMaria Reif and Martin Zacharias\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e19.1 Significance 501\u003c\/p\u003e \u003cp\u003e19.2 Theory and Principles 502\u003c\/p\u003e \u003cp\u003e19.3 Methodology 506\u003c\/p\u003e \u003cp\u003e19.4 Applications 525\u003c\/p\u003e \u003cp\u003e19.5 Concluding Remarks 528\u003c\/p\u003e \u003cp\u003eReferences 529\u003c\/p\u003e \u003cp\u003eFurther Reading 535\u003c\/p\u003e \u003cp\u003eIndex 537\u003c\/p\u003e  \t\u003cp\u003e\u003cb\u003eEditor:\u003c\/b\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003eDr Vasudevan Ramesh\u003c\/b\u003e, retired from the Faculty of the School of Chemistry at the University of Manchester, Manchester, UK, after 18 years of distinguished service. He taught Physical Chemistry at all degree levels and pursued an active research programme in Biomolecular NMR spectroscopy. He was a Leverhulme Research Fellow at the University of Leicester and a Visiting Lecturer and Annual Award winner at the Peking University, Beijing, China.   \u003c\/p\u003e\u003cp\u003e\u003cb\u003eAn essential guide to biomolecular and bioanalytical techniques and their applications\u003c\/b\u003e \u003c\/p\u003e\u003cp\u003eB\u003ci\u003eiomolecular and Bioanalytical Techniques\u003c\/i\u003e offers an introduction to, and a basic understanding of, a wide range of biophysical techniques. The text takes an interdisciplinary approach with contributions from a panel of distinguished experts. With a focus on research, the text comprehensively covers a broad selection of topics drawn from contemporary research in the fields of chemistry and biology. Each of the internationally reputed authors has contributed a single chapter on a specific technique. The chapters cover the specific technique's background, theory, principles, technique, methodology, protocol and applications. \u003c\/p\u003e\u003cp\u003eThe text explores the use of a variety of analytical tools to characterise biological samples. The contributors explain how to identify and quantify biochemically important molecules, including small molecules as well as biological macromolecules such as enzymes, antibodies, proteins, peptides and nucleic acids. This book is filled with essential knowledge and explores the skills needed to carry out the research and development roles in academic and industrial laboratories. \u003c\/p\u003e\u003cul\u003e \u003cli\u003eA technique-focused book that bridges the gap between an introductory text and a book on advanced research methods\u003c\/li\u003e \u003cli\u003eProvides the necessary background and skills needed to advance the research methods\u003c\/li\u003e \u003cli\u003eFeatures a structured approach within each chapter\u003c\/li\u003e \u003cli\u003eDemonstrates an interdisciplinary approach that serves to develop independent thinking\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eWritten for students in chemistry, biological, medical, pharmaceutical, forensic and biophysical sciences, \u003ci\u003eBiomolecular and Bioanalytical Techniques\u003c\/i\u003e is an in-depth review of the most current biomolecular and bioanalytical techniques in the field.\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47988839874789,"sku":"NP9781119483960","price":144.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9781119483960.jpg?v=1761781726","url":"https:\/\/k12savings.com\/products\/biomolecular-and-bioanalytical-techniques-isbn-9781119483960","provider":"K12savings","version":"1.0","type":"link"}