Atlas of Comparative Diagnostic and Experimental Hematology

A vital resource on blood and bone marrow cell morphology in laboratory animal medicine. This fully revised new edition is an essential reference for clinical pathologists in diagnostic laboratories, and medical or veterinary research. The atlas contains over 400 color images of cells from the peripheral blood and bone marrow from a variety of animals encountered in laboratory animal medicine, in health and disease.

Key features:

• New chapter on flow cytometry and its application in terms of routine analyses as a means of identifying abnormalities in cell marker expression, which is of particular relevance for pre-clinical safety assessment
• Covers the most recent developments in laboratory animal hematology, including parameters measured by the latest generation of analyzers
• Coverage of a wide range of laboratory animal species, as well as those used in clinical veterinary trials
• Photomicrographs present normal and abnormal blood cells from a variety of hematological conditions along with descriptive text

Foreword vii

Introduction ix

Dedication xi

Acknowledgments xiii

1 Rodents 1

Mouse 1

Introduction 1

Blood picture 1

Typical ranges (Siemens Advia 120) 2

Hamster 15

Introduction 15

Blood picture 15

Guinea Pig 17

Introduction 17

Blood picture 17

Rat – Sprague Dawley (SD)/Han Wistar (HW) 18

Introduction 18

Blood picture 18

Typical ranges (Siemens Advia 120) 19

References 36

2 Rabbit 37

Introduction 37

Blood picture 37

References 39

4 Dog 45

Introduction 45

Blood picture 45

Typical ranges (Siemens Advia 120) 46

References 64

5 Monkey 65

Introduction 65

Blood picture 65

Marmoset 66

Cynomolgus 71

Introduction 71

Blood picture 71

Typical ranges (Siemens Advia 120) 72

Baboon 84

Rhesus Monkey 86

References 87

6 Other species 89

Pig 89

Introduction 89

Blood picture 89

Horse 91

Introduction 91

Blood picture 91

Goat and Sheep 94

Assorted Other Species 96

References 103

3 Cat 41

Introduction 41

Blood picture 41

References 44

7 Blood collection procedures 105

Common bleeding sites 105

Anticoagulants 105

Staining 105

8 Artifacts 107

Collection artifacts 107

Sample transport and storage 109

Anticoagulant 110

Storage artifacts 111

Blood smear preparation 113

Stain precipitate 113

References 114

9 Bone marrow 115

Introduction 115

Review of marrow sampling techniques 115

Recommended methods for marrow sampling 116

Preparation and staining of smears 118

Evaluation 119

Interpretation 119

References 120

10 Comparative applications in flow cytometry 121

Application of flow cytometry in comparative hematology 122

References 132

Index 135

“This book may not be useful for the typical veterinary student or practitioner, but it is an excellent resource for those involved in preclinical studies on toxicosis.” (Journal of The American Veterinary Medical Association, 15 June 2012)

"A fine college reference!." (The Midwest Book Review, 1 November 2011) Clifford Smith is Chief Laboratory Scientist in the Haematology Department, and Head of Logistics, Clinical Pathology Services, at Covance Laboratories in Harrogate, UK.

Alfred Jarecki is Principal Research Scientist with Sanofi-Aventis at the Alnwick Research Centre, UK.

A vital resource on blood and bone marrow cell morphology in laboratory animals, this is an essential reference for clinical pathologists in diagnostic laboratories and in medical or veterinary research. It contains over 400 high quality color images of normal and abnormal cells from the peripheral blood and bone marrow from a variety of animals encountered in laboratory settings. These are structured in chapters by species, and accompanied by clear explanatory notes. This fully revised new edition covers the most recent developments in laboratory animal hematology, including parameters measured by the latest generation of analyzers.

KEY FEATURES

• Contains over 400 full color photographs of normal and abnormal blood cells along with descriptive text
• Includes new chapter on flow cytometry and its application as a means of identifying abnormalities in cell marker expression
• Covers a wide range of laboratory animal species, as well as those used in clinical veterinary trials
• Reflects most recent developments in the field with parameters measured by the latest generation of analyzers