{"product_id":"assay-development-isbn-9780470191156","title":"Assay Development","description":"\u003cp\u003eEssential principles and practice of assay development\u003c\/p\u003e \u003cp\u003eThe first comprehensive, integrated treatment of the subject, Assay Development: Fundamentals and Practices covers the essentials and techniques involved in carrying out an assay project in either a biotechnology\/drug discovery setting or a platform setting.\u003c\/p\u003e \u003cp\u003eRather than attempting comprehensive coverage of all assay development technologies, the book introduces the most widely used assay development technologies and illustrates the art of assay development through a few commonly encountered biological targets in assay development (e.g., proteases, kinases, ion channels, and G protein-coupled receptors). Just enough biological background for these biological targets is provided so that the reader can follow the logics of assay development. Chapters discuss:\u003c\/p\u003e \u003cul\u003e \u003cli\u003e \u003cp\u003eThe basics of assay development, including foundational concepts and applications\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eCommonly used instrumental methods for both biochemical assays and cell-based assays\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eAssay strategies for protein binding and enzymatic activity\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eCell-based assays\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eHigh-throughput screening\u003c\/p\u003e \u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eAn in-depth study of the now popular Caliper's off-chip kinase assay provides an instructive, real-world example of the assay development process.\u003c\/p\u003e  Preface.  \u003cp\u003eChapter 1 Introduction to assay development.\u003c\/p\u003e \u003cp\u003e1.1 Assay and Bioassay.\u003c\/p\u003e \u003cp\u003e1.2 Drug discovery process and the role of assays in the process.\u003c\/p\u003e \u003cp\u003e1.3 Bioassay development.\u003c\/p\u003e \u003cp\u003e1.4 Bioassay Classifications.\u003c\/p\u003e \u003cp\u003eChapter 2 Measurement and Instrumentation.\u003c\/p\u003e \u003cp\u003e2.1 Measurement and perturbation..\u003c\/p\u003e \u003cp\u003e2.2 Common instrumental methods and instrument components.\u003c\/p\u003e \u003cp\u003e2.3 Molecular absorption measurements.\u003c\/p\u003e \u003cp\u003e2.4 Molecular luminescence measurements.\u003c\/p\u003e \u003cp\u003e2.5 Luminescence lifetime measurement and time-resolved fluorescence (TRF) measurement..\u003c\/p\u003e \u003cp\u003e2.6 Fluorescence resonance energy transfer (FRET) and TR-FRET.\u003c\/p\u003e \u003cp\u003e2.7 Fluorescence quenching.\u003c\/p\u003e \u003cp\u003e2.8 Fluorescence polarization.\u003c\/p\u003e \u003cp\u003e2.9 Radioactivity measurement.\u003c\/p\u003e \u003cp\u003e2.10 Evaluating and selecting an instrumental method for bioassay.\u003c\/p\u003e \u003cp\u003eChapter 3 Fundamental principles of assay with isolated proteins.\u003c\/p\u003e \u003cp\u003e3.1 Chemical potential, equilibrium, and kinetics.\u003c\/p\u003e \u003cp\u003e3.2 Protein binding studies at equilibrium.\u003c\/p\u003e \u003cp\u003e3.3 Kinetic studies of protein binding process.\u003c\/p\u003e \u003cp\u003e3.4 Enzyme kinetics.\u003c\/p\u003e \u003cp\u003e3.5 Inhibition of protein function.\u003c\/p\u003e \u003cp\u003e3.6 Assay development with isolated proteins.\u003c\/p\u003e \u003cp\u003eChapter 4 Separation-based techniques in bioassays.\u003c\/p\u003e \u003cp\u003e4.1 Washing to remove impurity on solid support.\u003c\/p\u003e \u003cp\u003e4.2 Organic extraction of hydrophobic molecules.\u003c\/p\u003e \u003cp\u003e4.3 Centrifugation to remove dense particles.\u003c\/p\u003e \u003cp\u003e4.4 Membrane filtration.\u003c\/p\u003e \u003cp\u003e4.5 Liquid chromatography.\u003c\/p\u003e \u003cp\u003e4.6 Electrophoresis.\u003c\/p\u003e \u003cp\u003eChapter 5 General protein binding assay formats.\u003c\/p\u003e \u003cp\u003e5.1 Equilibrium dialysis.\u003c\/p\u003e \u003cp\u003e5.2 Competitive binding assays with radioactive or other labeled ligands.\u003c\/p\u003e \u003cp\u003e5.3 Application of SPATM and FlashplateTM in binding studies.\u003c\/p\u003e \u003cp\u003e5.4 Application of FP assays in binding studies.\u003c\/p\u003e \u003cp\u003e5.5 Application of FRET assays in binding studies.\u003c\/p\u003e \u003cp\u003e5.6 Application of ELISA in binding studies.\u003c\/p\u003e \u003cp\u003e5.7 Surface plasmon resonance (SPR) technology and its application in binding studies.\u003c\/p\u003e \u003cp\u003e5.8 Application of label-free technologies in binding studies.\u003c\/p\u003e \u003cp\u003eChapter 6 Functional assays with isolated proteases.\u003c\/p\u003e \u003cp\u003e6.1 Introduction to proteases and their substrates.\u003c\/p\u003e \u003cp\u003e6.2 The function of proteases and their role drug discovery.\u003c\/p\u003e \u003cp\u003e6.3 Protease assays.\u003c\/p\u003e \u003cp\u003e6.4 Protease substrate profiling.\u003c\/p\u003e \u003cp\u003e6.5 Protease inhibitors.\u003c\/p\u003e \u003cp\u003e6.6 Assay development for caspases with fluorogenic substrate..\u003c\/p\u003e \u003cp\u003e6.7 Assay development for Carboxypeptidase U..\u003c\/p\u003e \u003cp\u003eChapter 7 Functional assays with isolated kinases.\u003c\/p\u003e \u003cp\u003e7.1 Introduction to protein kinases.\u003c\/p\u003e \u003cp\u003e7.2 Substrates for in vitro kinase assays.\u003c\/p\u003e \u003cp\u003e7.3 Kinase assay development strategies.\u003c\/p\u003e \u003cp\u003e7.4 Kinase assay based on the detection of the phosphorylated product.\u003c\/p\u003e \u003cp\u003e7.5 Kinase Assays by measuring the generation of ADP.\u003c\/p\u003e \u003cp\u003e7.6 Kinase assays by measuring the depletion of ATP.\u003c\/p\u003e \u003cp\u003e7.7 Kinase assays by measuring the depletion of peptide substrate.\u003c\/p\u003e \u003cp\u003e7.8 Kinase assays by measurement of both product and substrate simultaneously.\u003c\/p\u003e \u003cp\u003e7.9 An example of a kinase assay development in HTRF format..\u003c\/p\u003e \u003cp\u003eChapter 8 Fundamental principles of cell-based assays.\u003c\/p\u003e \u003cp\u003e8.1 Cell signaling, signal transduction, and cellular responses.\u003c\/p\u003e \u003cp\u003e8.2 General approaches in cell-based assays.\u003c\/p\u003e \u003cp\u003e8.3 The concept of affinity and efficacy in cell-based assays.\u003c\/p\u003e \u003cp\u003e8.4 Development of cell-based assays.\u003c\/p\u003e \u003cp\u003eChapter 9 Assays with ion channels.\u003c\/p\u003e \u003cp\u003e9.1 Introduction to ion channels.\u003c\/p\u003e \u003cp\u003e9.2 Strategies for ion channel assays.\u003c\/p\u003e \u003cp\u003e9.3 Electrophysiological methods.\u003c\/p\u003e \u003cp\u003e9.4 Ion flux methods.\u003c\/p\u003e \u003cp\u003e9.5 Membrane potential sensing methods.\u003c\/p\u003e \u003cp\u003e9.6 Selecting suitable assays for ion channel studies.\u003c\/p\u003e \u003cp\u003eChapter 10 Assays with G protein-coupled receptors.\u003c\/p\u003e \u003cp\u003e10.1 Introduction to GPCRs and G proteins.\u003c\/p\u003e \u003cp\u003e10.2. GPCR activation and signal transduction.\u003c\/p\u003e \u003cp\u003e10.3 Strategies of GPCR assay development.\u003c\/p\u003e \u003cp\u003e10.4 GPCR assays by measuring the extent of GTP binding to G?.\u003c\/p\u003e \u003cp\u003e10.5 GPCR assays based on measurement of cAMP.\u003c\/p\u003e \u003cp\u003e10.6 GPCR assays based on the measurement of intracellular inositol phospholipids.\u003c\/p\u003e \u003cp\u003e10.7 GPCR assays based on the measurement of intracellular Ca2+.\u003c\/p\u003e \u003cp\u003e10.8. GPCR assays based on the measurement of MAPK activity.\u003c\/p\u003e \u003cp\u003e10.9. GPCR assays with reporter gene.\u003c\/p\u003e \u003cp\u003e10.10 GPCR assays by monitoring events leading to GPCR internalization.\u003c\/p\u003e \u003cp\u003eChapter 11 Assays based on the integrated system properties of cells.\u003c\/p\u003e \u003cp\u003e11.1. Cell viability, proliferation, and cytotoxicity assays.\u003c\/p\u003e \u003cp\u003e11.2. Measurement of extracellular indicators of cellular metabolism.\u003c\/p\u003e \u003cp\u003e11.3. Measurement of cell?s effect on electrical impedance.\u003c\/p\u003e \u003cp\u003e11.4. Measurement of protein secretion from cells.\u003c\/p\u003e \u003cp\u003e11.5. Measurement of discoloration of melanophore cells.\u003c\/p\u003e \u003cp\u003e11.6. Measurement of cell motility.\u003c\/p\u003e \u003cp\u003eChapter 12 High content cell-based assay with optical imaging techniques.\u003c\/p\u003e \u003cp\u003e12.1 Sample preparation.\u003c\/p\u003e \u003cp\u003e12.2 Cellular image collection.\u003c\/p\u003e \u003cp\u003e12.3 Image abstraction, analysis, and data management.\u003c\/p\u003e \u003cp\u003e12.4 Applications of image-based cellular high content screening.\u003c\/p\u003e \u003cp\u003eChapter 13 High throughput screening.\u003c\/p\u003e \u003cp\u003e13.1 Introduction to high throughput screening.\u003c\/p\u003e \u003cp\u003e13.2 Molecular or cellular target and assay development.\u003c\/p\u003e \u003cp\u003e13.3 Compound library management.\u003c\/p\u003e \u003cp\u003e13.4 Hardware module.\u003c\/p\u003e \u003cp\u003e13.5 Software module.\u003c\/p\u003e \u003cp\u003e13.6 HTS operation management.\u003c\/p\u003e \u003cp\u003e13.7 Building an HTS operation for biopharmaceutical discovery.\u003c\/p\u003e \u003cp\u003e13.8 Quality control and data analysis in primary screening.\u003c\/p\u003e \u003cp\u003eChapter 14 A case study: The development of a microfluidic-based kinase assay platform.\u003c\/p\u003e \u003cp\u003e14.1 Background of microfluidic technology and its application in bioassays.\u003c\/p\u003e \u003cp\u003e14.2 The original Caliper?s mobility shift kinase assay format.\u003c\/p\u003e \u003cp\u003e14.3 Realizing the flaws in Caliper?s original kinase assay format.\u003c\/p\u003e \u003cp\u003e14.4 Searching for alternative kinase assay methods.\u003c\/p\u003e \u003cp\u003e14.5 The development of the off-chip kinase assay format.\u003c\/p\u003e \u003cp\u003e14.6 Current stage of microfluidic technology in bioassays.\u003c\/p\u003e \u003cp\u003e14.7 Appendix: A poster presented in 2002 Society for Biomolecular Screening annual meeting.\u003c\/p\u003e \"Although it is not an easy task to satisfy the demands of all scientists, the book Assay Development is an important treaise for scientits of modern biology\". (ChemBioChem, 7 March 2011)  \u003cp\u003eGe Wu, PhD, is Director of Assay Development at Iris International. He obtained his doctorate in biochemistry from UCLA, performed postdoctoral work at Harvard Medical School, and was a member of the faculty at Cornell University Medical College. Prior to joining Iris International, he worked at Merck \u0026amp; Co., Caliper Technologies, Amphora Discovery, and FivePrime Therapeutics. He is the founding Director of Assay Development and Screening at FivePrime Therapeutics. Dr. Wu is a leading figure in assay development and screening in biopharmaceutical discovery using primary cell assay and the author of an acclaimed review in this subject.\u003c\/p\u003e  \u003cp\u003eEssential principles and practice of assay development\u003c\/p\u003e \u003cp\u003eThe first comprehensive, integrated treatment of the subject, Assay Development: Fundamentals and Practices covers the essentials and techniques involved in carrying out an assay project in either a biotechnology\/drug discovery setting or a platform setting.\u003c\/p\u003e \u003cp\u003eRather than attempting comprehensive coverage of all assay development technologies, the book introduces the most widely used assay development technologies and illustrates the art of assay development through a few commonly encountered biological targets in assay development (e.g., proteases, kinases, ion channels, and G protein-coupled receptors). Just enough biological background for these biological targets is provided so that the reader can follow the logics of assay development. Chapters discuss:\u003c\/p\u003e \u003cul\u003e \u003cli\u003e \u003cp\u003eThe basics of assay development, including foundational concepts and applications\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eCommonly used instrumental methods for both biochemical assays and cell-based assays\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eAssay strategies for protein binding and enzymatic activity\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eCell-based assays\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eHigh-throughput screening\u003c\/p\u003e \u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eAn in-depth study of the now popular Caliper's off-chip kinase assay provides an instructive, real-world example of the assay development process.\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47988768080101,"sku":"NP9780470191156","price":188.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9780470191156.jpg?v=1761781516","url":"https:\/\/k12savings.com\/products\/assay-development-isbn-9780470191156","provider":"K12savings","version":"1.0","type":"link"}