{"product_id":"antigen-retrieval-immunohistochemistry-based-research-and-diagnostics-isbn-9780470624524","title":"Antigen Retrieval Immunohistochemistry Based Research and Diagnostics","description":"\u003cb\u003eThe most complete, up-to-date reference on antigen retrieval and immunohistochemistry\u003c\/b\u003e  \u003cp\u003eAn antigen is a substance that prompts the generation of antibodies and can cause an immune response. The antigen retrieval (AR) technique is in wide use across the globe, and is a critical technique used in medical diagnosis of disease, particularly clinical targeted cancer treatment.\u003c\/p\u003e \u003cp\u003e\u003ci\u003eAntigen Retrieval Immunohistochemistry Based Research and Diagnostics\u003c\/i\u003e discusses several scientific approaches to the standardization of quantifiable immunohistochemistry (IHC). Based on the development and application of AR by the editors, this volume summarizes recent achievements in AR-IHC and analyzes numerous cutting-edge issues for future research projects.\u003c\/p\u003e \u003cp\u003eFeaturing contributions from a worldwide group of leading experts and research scientists in the field, this important work:\u003c\/p\u003e \u003cul\u003e \u003cli\u003eSummarizes the key problems in the four fields of antigen retrieval\u003c\/li\u003e \u003cli\u003eDiscusses the advances of AR techniques and their applications\u003c\/li\u003e \u003cli\u003eProvides practical methods and protocols in AR-IHC, such as extraction of nucleic acids and proteins for molecular analysis, cell\/tissue sample preparation, and standardization and development of various techniques to meet the future needs of¿clinical and research molecular analysis\u003c\/li\u003e \u003cli\u003eEncourages further research in AR and IHC, particularly how AR methods might be employed for improved test performance and the development of greater reliability and reproducibility of IHC\u003c\/li\u003e \u003cli\u003eIncludes an appendix of related laboratory protocols\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003ci\u003eAntigen Retrieval Immunohistochemistry Based Research and Diagnostics\u003c\/i\u003e is intended for clinical pathologists, molecular cell biologists, basic research scientists, technicians, and graduate students who undertake tissue\/cell morphologic and molecular analysis and wish to use and extend the power of immunohistochemistry. It is also pertinent for most biotechnology companies majoring in development of IHC products.\u003c\/p\u003e \u003cp\u003e\u003cb\u003eWiley Series in Biomedical Engineering and Multi-Disciplinary Integrated Systems \/ Kai Chang, Series Editor\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003ePreface ix\u003c\/p\u003e \u003cp\u003eContributors xv\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart I Recent Advances in Antigen Retrieval Techniques and Its Application 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1 Standardization of Antigen Retrieval Techniques Based on the Test Battery Approach 3\u003cbr\u003e \u003ci\u003eShan-Rong Shi and Clive R. Taylor\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2 Extended Application of Antigen Retrieval Technique in Immunohistochemistry and in Situ\u003cbr\u003e Hybridization 25\u003cbr\u003e \u003ci\u003eShan-Rong Shi and Clive R. Taylor\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3 Extraction of Dna\/rna From Formalin-fixed, Paraffin-embedded Tissue Based on the Antigen Retrieval Principle 47\u003cbr\u003e \u003ci\u003eShan-Rong Shi and Clive R. Taylor\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart II Standardization of Immunohistochemistry 73\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4 Key Issues and Strategies of Standardization For Quantifiable Immunohistochemistry 75\u003cbr\u003e \u003ci\u003eShan-Rong Shi, Kevin A. Roth, and Clive R. Taylor\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5 Standardization of Immunohistochemistry Based on Antigen Retrieval Technique 87\u003cbr\u003e \u003ci\u003eShan-Rong Shi and Clive R. Taylor\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6 Standard Reference Material: Cell Line Development and Use of Reference Cell Lines as Standards for External Quality Assurance of Her2 Ihc and Ish Testing 101\u003cbr\u003e \u003ci\u003eBharat Jasani, Vicky Reid, Colin Tristram, Jeremy Walker, Paul Scorer, Michael Morgan, John Bartlett, Merdol Ibrahim, and Keith Miller\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7 Peptides as Immunohistochemistry Controls 123\u003cbr\u003e \u003ci\u003eSteven A. Bogen and Seshi R. Sompuram\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e8 Standard Reference Material: Protein- Embedding Technique and Design of Bar Code 141\u003cbr\u003e \u003ci\u003eShan-Rong Shi, Jiang Gu, and Clive R. Taylor\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e9 the Pros and Cons of Automation for Immunohistochemistry From the Prospective Of the Pathology Laboratory 151\u003cbr\u003e \u003ci\u003eDavid G. Hicks and Loralee McMahon\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e10 Image Analysis in Immunohistochemistry 165\u003cbr\u003e \u003ci\u003eAlton D. Floyd\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart III Tissue\/cell Sample Preparation 187\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e11 Tissue Cell Sample Preparation: Lessons From the Antigen Retrieval Technique 189\u003cbr\u003e \u003ci\u003eShan-Rong Shi and Clive R. Taylor\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e12 Mechanisms of Action and Proper Use of Common Fixatives 195\u003cbr\u003e \u003ci\u003eRichard W. Dapson\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e13 Cell Sample Preparation for Clinical Cytopathology: Current Status and Future Development 219\u003cbr\u003e \u003ci\u003eYan Shi and Patricia G. Wasserman\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e14 Design of a Tissue Surrogate to Examine Accuracy of Proteomic Analysis 235\u003cbr\u003e \u003ci\u003eCarol B. Fowler, Jeffrey T. Mason, and Timothy J. O’leary\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart IV Molecular Mechanism of Antigen Retrieval Technique 251\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e15 Study of Formalin Fixation and Heat-induced Antigen Retrieval 253\u003cbr\u003e \u003ci\u003eJeffrey T. Mason, Carol B. Fowler, and Timothy J. O’leary\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e16 a Linear Epitopes Model of Antigen Retrieval 287\u003cbr\u003e \u003ci\u003eSteven A. Bogen and Seshi R. Sompuram\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e17 Ph Or Ionic Strength of Antigen Retrieval Solution: a Potential Role for Refolding During Heat Treatment 303\u003cbr\u003e \u003ci\u003eShuji Yamashita\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e18 Commentary: Future Directions 323\u003cbr\u003e \u003ci\u003eTimothy J. O’Leary, Carol B. Fowler, David L. Evers, Robert E. Cunningham, and Jeffrey T. Mason\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart V Proteomic Analysis of Protein Extracted From Tissue\/cells 333\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e19 Techniques of Protein Extraction From Ffpe Tissue\/cells for Mass Spectrometry 335\u003cbr\u003e \u003ci\u003eCarol B. Fowler, Timothy J. O’Leary, and Jeffrey T. Mason\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e20 Application of Shotgun Proteomics to Formalin-fixed and Paraffin-embedded Tissues 347\u003cbr\u003e \u003ci\u003eBrian M. Balgley\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e21 Visualizing Protein Maps in Tissue 369\u003cbr\u003e \u003ci\u003eMasahiro Mukai and Mitsutoshi Setou\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e22 Symbiosis of Immunohistochemistry and Proteomics: Marching to a New Era 391\u003cbr\u003e \u003ci\u003eShan-Rong Shi, Brian M. Balgley, and Clive R. Taylor\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eAppendix Related Laboratory Protocols 399\u003c\/p\u003e \u003cp\u003eIndex 413\u003c\/p\u003e  \u003cb\u003eShan-Rong Shi, MD,\u003c\/b\u003e is professor of clinical pathology at the Keck School of Medicine of the University of Southern California. He is the Associate Editor of The Journal of Histochemistry and cytochemistry, he is on the Editoral Board of Applied Immunohistochemistry and Molecular Morphology and is also on the Overseas Editorial Board of the Chinese Journal of Pathology. Dr. Shi is the ineventor of the antigen retrieval (AR) technique.  \u003cp\u003e\u003cb\u003eClive R. Taylor, MD, PhD\u003c\/b\u003e, is Senior Associate Dean, Professor and Chairman of Pathology at the Keck School of Medicine. Dr. Taylor is Editor in Chief of Applied Immunohistochemistry and Molecular Morphology. Dr. Taylor is a world renowned expert in immunohistochemistry and clinical pathology.\u003c\/p\u003e  \u003cb\u003eThe most complete, up-to-date reference on antigen retrieval and immunohistochemistry\u003c\/b\u003e  \u003cp\u003eAn antigen is a substance that prompts the generation of antibodies and can cause an immune response. The antigen retrieval (AR) technique is in wide use across the globe, and is a critical technique used in medical diagnosis of disease, particularly clinical targeted cancer treatment.\u003c\/p\u003e \u003cp\u003e\u003ci\u003eAntigen Retrieval Immunohistochemistry Based Research and Diagnostics\u003c\/i\u003e discusses several scientific approaches to the standardization of quantifiable immunohistochemistry (IHC). Based on the development and application of AR by the editors, this volume summarizes recent achievements in AR-IHC and analyzes numerous cutting-edge issues for future research projects.\u003c\/p\u003e \u003cp\u003eFeaturing contributions from a worldwide group of leading experts and research scientists in the field, this important work:\u003c\/p\u003e \u003cul\u003e \u003cli\u003eSummarizes the key problems in the four fields of antigen retrieval\u003c\/li\u003e \u003cli\u003eDiscusses the advances of AR techniques and their applications\u003c\/li\u003e \u003cli\u003eProvides practical methods and protocols in AR-IHC, such as extraction of nucleic acids and proteins for molecular analysis, cell\/tissue sample preparation, and standardization and development of various techniques to meet the future needs of¿clinical and research molecular analysis\u003c\/li\u003e \u003cli\u003eEncourages further research in AR and IHC, particularly how AR methods might be employed for improved test performance and the development of greater reliability and reproducibility of IHC\u003c\/li\u003e \u003cli\u003eIncludes an appendix of related laboratory protocols\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003ci\u003eAntigen Retrieval Immunohistochemistry Based Research and Diagnostics\u003c\/i\u003e is intended for clinical pathologists, molecular cell biologists, basic research scientists, technicians, and graduate students who undertake tissue\/cell morphologic and molecular analysis and wish to use and extend the power of immunohistochemistry. It is also pertinent for most biotechnology companies majoring in development of IHC products.\u003c\/p\u003e \u003cp\u003e\u003cb\u003eWiley Series in Biomedical Engineering and Multi-Disciplinary Integrated Systems \/ Kai Chang, Series Editor\u003c\/b\u003e\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47988745797861,"sku":"NP9780470624524","price":188.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9780470624524.jpg?v=1761781425","url":"https:\/\/k12savings.com\/products\/antigen-retrieval-immunohistochemistry-based-research-and-diagnostics-isbn-9780470624524","provider":"K12savings","version":"1.0","type":"link"}