{"product_id":"understanding-light-microscopy-isbn-9780470973752","title":"Understanding Light Microscopy","description":"\u003cp\u003e\u003cb\u003eIntroduces readers to the enlightening world of the modern light microscope\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eThere have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. \u003c\/p\u003e \u003cp\u003eWritten to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, \u003ci\u003eUnderstanding Light Microscopy\u003c\/i\u003e keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP \u0026amp; FRET; super-resolution techniques; biological and materials specimen preparation; and more.\u003c\/p\u003e \u003cul\u003e \u003cli\u003eGives a didactic introduction to the light microscope\u003c\/li\u003e \u003cli\u003eEncourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility\u003c\/li\u003e \u003cli\u003eFeatures full-colour illustrations and workable practical protocols\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003ci\u003eUnderstanding Light Microscopy\u003c\/i\u003e is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.\u003c\/p\u003e \u003cp\u003eAbout the Author ix\u003c\/p\u003e \u003cp\u003eAcknowledgements xi\u003c\/p\u003e \u003cp\u003eLook-Up Guide to Feature Boxes by Theme xii\u003c\/p\u003e \u003cp\u003eGlossary and Definitions xiv\u003c\/p\u003e \u003cp\u003eNotes xxiv\u003c\/p\u003e \u003cp\u003eIntroduction xxvii\u003c\/p\u003e \u003cp\u003e1 Our Eyes and the Microscope 1\u003c\/p\u003e \u003cp\u003e2 Light 29\u003c\/p\u003e \u003cp\u003e3 Basic Microscope Optics 55\u003c\/p\u003e \u003cp\u003e4 Microscope Anatomy and Design 75\u003c\/p\u003e \u003cp\u003e5 Ergonomics 91\u003c\/p\u003e \u003cp\u003e6 Optical Aberrations of the Microscope 101\u003c\/p\u003e \u003cp\u003e7 The Microscope Objective 127\u003c\/p\u003e \u003cp\u003e8 Condensers and Eyepieces 161\u003c\/p\u003e \u003cp\u003e9 Illumination in the Microscope 177\u003c\/p\u003e \u003cp\u003e10 Diffraction and Image Formation in Microscopy 211\u003c\/p\u003e \u003cp\u003e11 Contrast Generation and Enhancement 243\u003c\/p\u003e \u003cp\u003e12 Reflected-Light Microscopy 289\u003c\/p\u003e \u003cp\u003e13 Polarised-Light Microscopy: Part 1 – Theory 317\u003c\/p\u003e \u003cp\u003e14 Polarised-Light Microscopy: Part 2 – Applied 347\u003c\/p\u003e \u003cp\u003e15 Fluorescence Microscopy 383\u003c\/p\u003e \u003cp\u003e16 Fluorophores and Fluorescent Proteins 405\u003c\/p\u003e \u003cp\u003e17 Optical Sectioning and Confocal Microscopy 425\u003c\/p\u003e \u003cp\u003e18 Operating the Confocal Microscope 447\u003c\/p\u003e \u003cp\u003e19 Light-Sheet Microscopy 483\u003c\/p\u003e \u003cp\u003e20 Bleed-Through and Spectral Unmixing 507\u003c\/p\u003e \u003cp\u003e21 Deconvolution 523\u003c\/p\u003e \u003cp\u003e22 Multi-Photon Microscopy 543\u003c\/p\u003e \u003cp\u003e23 Total Internal Reflection Fluorescence Microscopy 561\u003c\/p\u003e \u003cp\u003e24 FRAP and FRET 569\u003c\/p\u003e \u003cp\u003e25 Colocalisation 587\u003c\/p\u003e \u003cp\u003e26 Super-Resolution Fluorescence Microscopy 613\u003c\/p\u003e \u003cp\u003e27 Choosing a Microscope Platform and Core Imaging Facilities 637\u003c\/p\u003e \u003cp\u003e28 Biological Specimen Preparation 663\u003c\/p\u003e \u003cp\u003e29 Materials Specimen Preparation 687\u003c\/p\u003e \u003cp\u003e30 Recording the Image: Part 1 – Theory 707\u003c\/p\u003e \u003cp\u003e31 Recording the Image: Part 2 – Applied 733\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendices\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1 Buying, and Tendering for, a Light Microscope 769\u003c\/p\u003e \u003cp\u003e2 Troubleshooting Poor Image Quality 773\u003c\/p\u003e \u003cp\u003e3 The Michel-Lévy Interference Colour Chart 775\u003c\/p\u003e \u003cp\u003e4 Cleaning and Maintenance of the Light Microscope 779\u003c\/p\u003e \u003cp\u003e5 Selected Suppliers 783\u003c\/p\u003e \u003cp\u003e6 Historical Background 787\u003c\/p\u003e \u003cp\u003e7 Timeline of Key Events 791\u003c\/p\u003e \u003cp\u003eIndex 799\u003c\/p\u003e  \u003cp\u003e\u003cb\u003eJEREMY SANDERSON\u003c\/b\u003e is a Bio-Imaging specialist at the Mammalian Genetics Unit, MRC Harwell Institute, in the UK. He is Chartered Scientist and a Fellow of the Royal Microscopical Society and has served both on the Executive Council and the LM Section Committee.   \u003c\/p\u003e\u003cp\u003e\u003cb\u003eINTRODUCES READERS TO THE ENLIGHTENING WORLD OF THE MODERN LIGHT MICROSCOPE\u003c\/b\u003e\t \t \u003c\/p\u003e\u003cp\u003eThere have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. \u003c\/p\u003e\u003cp\u003eWritten to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, \u003ci\u003eUnderstanding Light Microscopy\u003c\/i\u003e keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP \u0026amp; FRET; super-resolution techniques; biological and materials specimen preparation; and much more. \u003c\/p\u003e\u003cul\u003e \u003cli\u003ePresents a didactic introduction to the light microscope\u003c\/li\u003e \u003cli\u003eEncourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility\u003c\/li\u003e \u003cli\u003eFeatures full-colour illustrations and workable practical protocols\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003ci\u003eUnderstanding Light Microscopy\u003c\/i\u003e is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47990430761189,"sku":"NP9780470973752","price":202.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9780470973752.jpg?v=1761787799","url":"https:\/\/k12savings.com\/es\/products\/understanding-light-microscopy-isbn-9780470973752","provider":"K12savings","version":"1.0","type":"link"}