{"product_id":"the-science-of-stem-cells-isbn-9781119235156","title":"The Science of Stem Cells","description":"\u003cp\u003e\u003cb\u003eIntroduces all of the essential cell biology and developmental biology background for the study of stem cells\u003c\/b\u003e\u003cb\u003e \u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eThis book gives you all the important information you need to become a stem cell scientist. It covers the characterization of cells, genetic techniques for modifying cells and organisms, tissue culture technology, transplantation immunology, properties of pluripotent and tissue specific stem cells and, in particular, the relevant aspects of mammalian developmental biology. It dispels many misconceptions about stem cells—especially that they can be miracle cells that can cure all ills. The book puts emphasis on stem cell behavior in its biological context and on how to study it. Throughout, the approach is simple, direct, and logical, and evidence is given to support conclusions. \u003c\/p\u003e \u003cp\u003eStem cell biology has huge potential for advancing therapies for many distressing and recalcitrant diseases, and its potential will be realized most quickly when as many people as possible have a good grounding in the science of stem cells.\u003c\/p\u003e \u003cul\u003e \u003cli\u003eContent focused on the basic science underpinning stem cell biology\u003c\/li\u003e \u003cli\u003eCovers techniques of studying cell properties and cell lineage in vivo and in vitro\u003c\/li\u003e \u003cli\u003eExplains the basics of embryonic development and cell differentiation, as well as the essential cell biology processes of signaling, gene expression, and cell division\u003c\/li\u003e \u003cli\u003eIncludes instructor resources such as further reading and figures for downloading\u003c\/li\u003e \u003cli\u003eOffers an online supplement summarizing current clinical applications of stem cells\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eWritten by a prominent leader in the field, \u003ci\u003eThe Science of Stem Cells\u003c\/i\u003e is an ideal course book for advanced undergraduates or graduate students studying stem cell biology, regenerative medicine, tissue engineering, and other topics of science and biology. \u003c\/p\u003e \u003cp\u003ePreface xi\u003c\/p\u003e \u003cp\u003eAbout the Companion Website xiii\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 What is a Stem Cell? 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eStem Cell Markers 3\u003c\/p\u003e \u003cp\u003eLabel‐ Retention 4\u003c\/p\u003e \u003cp\u003eThe Niche 5\u003c\/p\u003e \u003cp\u003eAsymmetric Division and Differentiated Progeny 6\u003c\/p\u003e \u003cp\u003eClonogenicity and Transplantation 6\u003c\/p\u003e \u003cp\u003eIn Vivo Lineage Labeling 7\u003c\/p\u003e \u003cp\u003eConclusions 9\u003c\/p\u003e \u003cp\u003eFurther Reading 10\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Characterizing Cells 13\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eHistological and Anatomical Methods 13\u003c\/p\u003e \u003cp\u003eHistological Sections 13\u003c\/p\u003e \u003cp\u003eFixation 13\u003c\/p\u003e \u003cp\u003eSectioning 14\u003c\/p\u003e \u003cp\u003eStaining 14\u003c\/p\u003e \u003cp\u003eElectron Microscopy 15\u003c\/p\u003e \u003cp\u003eFluorescence Microscopy 16\u003c\/p\u003e \u003cp\u003eWholemounts 17\u003c\/p\u003e \u003cp\u003eImmunostaining 17\u003c\/p\u003e \u003cp\u003eIn Situ Hybridization 18\u003c\/p\u003e \u003cp\u003eOther Methods 19\u003c\/p\u003e \u003cp\u003eRNAseq 19\u003c\/p\u003e \u003cp\u003eLaser Capture Microdissection 19\u003c\/p\u003e \u003cp\u003eFlow Cytometry 20\u003c\/p\u003e \u003cp\u003eDividing Cells 21\u003c\/p\u003e \u003cp\u003eThe Cell Cycle 21\u003c\/p\u003e \u003cp\u003eStudying Cell Turnover 24\u003c\/p\u003e \u003cp\u003eReporters for the Cell Cycle 26\u003c\/p\u003e \u003cp\u003eIdentification of Very Slow Cell Turnover 26\u003c\/p\u003e \u003cp\u003eClassification of Cell Types by Proliferative Behavior 28\u003c\/p\u003e \u003cp\u003eCell Death 28\u003c\/p\u003e \u003cp\u003eFurther Reading 30\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Genetic Modification and the Labeling of Cell Lineages 31\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroducing Genes to Cells 31\u003c\/p\u003e \u003cp\u003eTransfection and Electroporation 31\u003c\/p\u003e \u003cp\u003eGene Delivery Viruses 33\u003c\/p\u003e \u003cp\u003eControlling Gene Expression 35\u003c\/p\u003e \u003cp\u003eTet System 35\u003c\/p\u003e \u003cp\u003eCre System 35\u003c\/p\u003e \u003cp\u003eInhibiting Gene Activity 37\u003c\/p\u003e \u003cp\u003eCRISPR‐Cas9 37\u003c\/p\u003e \u003cp\u003eTransgenic Mice 38\u003c\/p\u003e \u003cp\u003eAnimal Procedures 38\u003c\/p\u003e \u003cp\u003eModification of Embryonic Stem Cells 40\u003c\/p\u003e \u003cp\u003eTypes of Transgenic Mice 41\u003c\/p\u003e \u003cp\u003eCell Lineage 42\u003c\/p\u003e \u003cp\u003eCell Lineage, Fate Maps, Clonal Analysis 43\u003c\/p\u003e \u003cp\u003eUse of CreER for Lineage Analysis 44\u003c\/p\u003e \u003cp\u003eRetroviral Barcoding 46\u003c\/p\u003e \u003cp\u003eClonal Analysis in Humans 47\u003c\/p\u003e \u003cp\u003eFurther Reading 47\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Tissue Culture, Tissue Engineering and Grafting49\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eSimple Tissue Culture 51\u003c\/p\u003e \u003cp\u003eMedia 51\u003c\/p\u003e \u003cp\u003eContamination 53\u003c\/p\u003e \u003cp\u003eGrowth in Culture 54\u003c\/p\u003e \u003cp\u003eCryopreservation and Banking 55\u003c\/p\u003e \u003cp\u003eGMP Cultivation 56\u003c\/p\u003e \u003cp\u003eComplex Tissue Culture 56\u003c\/p\u003e \u003cp\u003eInduced Differentiation 56\u003c\/p\u003e \u003cp\u003eThree Dimensional Cell Culture 57\u003c\/p\u003e \u003cp\u003eArtificial Organs and Organoids 59\u003c\/p\u003e \u003cp\u003eGrafting 60\u003c\/p\u003e \u003cp\u003eThe Immune System 61\u003c\/p\u003e \u003cp\u003eT Cells 61\u003c\/p\u003e \u003cp\u003eThe Major Histocompatibility Complex 62\u003c\/p\u003e \u003cp\u003eT and B Cell Responses 63\u003c\/p\u003e \u003cp\u003eReactions to a Graft 64\u003c\/p\u003e \u003cp\u003eImmunosuppressive Drugs 65\u003c\/p\u003e \u003cp\u003eAnimal Experiments Involving Grafting 66\u003c\/p\u003e \u003cp\u003eFurther Reading 67\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Early Mouse and Human Development 69\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eGametogenesis 70\u003c\/p\u003e \u003cp\u003eGerm Cells 70\u003c\/p\u003e \u003cp\u003eMitosis and Meiosis 70\u003c\/p\u003e \u003cp\u003ePrimordial Germ Cells (PGCs) 72\u003c\/p\u003e \u003cp\u003eSpermatogenesis 73\u003c\/p\u003e \u003cp\u003eOogenesis 73\u003c\/p\u003e \u003cp\u003eFertilization 76\u003c\/p\u003e \u003cp\u003eEarly Development 77\u003c\/p\u003e \u003cp\u003ePreimplantation Phase 77\u003c\/p\u003e \u003cp\u003eImplantation Period – Mouse 80\u003c\/p\u003e \u003cp\u003eImplantation Period – Human 82\u003c\/p\u003e \u003cp\u003eEthical and Legal Issues Concerning the Early Human Conceptus 85\u003c\/p\u003e \u003cp\u003eSex Determination 86\u003c\/p\u003e \u003cp\u003eX‐Inactivation 87\u003c\/p\u003e \u003cp\u003eImprinting 87\u003c\/p\u003e \u003cp\u003eCloning by Nuclear Transplantation (SCNT) 89\u003c\/p\u003e \u003cp\u003eFurther Reading 90\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Pluripotent Stem Cells 93\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eMouse Pluripotent Stem Cells 93\u003c\/p\u003e \u003cp\u003eMouse Embryonic Stem Cells 93\u003c\/p\u003e \u003cp\u003eDifferentiation of Mouse ES cells 95\u003c\/p\u003e \u003cp\u003eMouse iPS Cells 97\u003c\/p\u003e \u003cp\u003eHuman Pluripotent Stem Cells 101\u003c\/p\u003e \u003cp\u003eSCNT‐Derived Embryonic Stem Cells 102\u003c\/p\u003e \u003cp\u003eEthical Issues Concerning Human ES Cells 102\u003c\/p\u003e \u003cp\u003ePluripotent Stem Cells from Postnatal Organisms 103\u003c\/p\u003e \u003cp\u003eApplications of Pluripotent Stem Cells 104\u003c\/p\u003e \u003cp\u003eFurther Reading 105\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Body Plan Formation 107\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eEmbryological Concepts 107\u003c\/p\u003e \u003cp\u003eDevelopmental Commitment 107\u003c\/p\u003e \u003cp\u003eEmbryonic Induction 109\u003c\/p\u003e \u003cp\u003eSymmetry Breaking 110\u003c\/p\u003e \u003cp\u003eKey Molecules Controlling Development 111\u003c\/p\u003e \u003cp\u003eGenes Encoding Developmental Commitment 111\u003c\/p\u003e \u003cp\u003eInducing Factors 112\u003c\/p\u003e \u003cp\u003eWnt System 112\u003c\/p\u003e \u003cp\u003eFGF System 113\u003c\/p\u003e \u003cp\u003eNodals and BMPs 114\u003c\/p\u003e \u003cp\u003eNotch System 114\u003c\/p\u003e \u003cp\u003eHedgehog System 115\u003c\/p\u003e \u003cp\u003eGrowth Promoting Pathways 115\u003c\/p\u003e \u003cp\u003eRetinoic Acid 115\u003c\/p\u003e \u003cp\u003eBody Plan Formation 116\u003c\/p\u003e \u003cp\u003eGeneral Body Plan 116\u003c\/p\u003e \u003cp\u003eGastrulation 116\u003c\/p\u003e \u003cp\u003eEmbryo Folding 120\u003c\/p\u003e \u003cp\u003eFurther Reading 123\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Organogenesis 125\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eNervous System 125\u003c\/p\u003e \u003cp\u003eThe Brain 126\u003c\/p\u003e \u003cp\u003eRegional Specification of the CNS 128\u003c\/p\u003e \u003cp\u003eRostrocaudal 128\u003c\/p\u003e \u003cp\u003eMediolateral 130\u003c\/p\u003e \u003cp\u003eDorsoventral 131\u003c\/p\u003e \u003cp\u003eThe Eye 131\u003c\/p\u003e \u003cp\u003eThe Neural Crest 132\u003c\/p\u003e \u003cp\u003eEpidermis 134\u003c\/p\u003e \u003cp\u003eHair Follicles 135\u003c\/p\u003e \u003cp\u003eMammary Glands 136\u003c\/p\u003e \u003cp\u003eSomitogenesis 137\u003c\/p\u003e \u003cp\u003eThe Somite Oscillator and Gradient 138\u003c\/p\u003e \u003cp\u003eSubdivision of the Somites 139\u003c\/p\u003e \u003cp\u003eMyogenesis 140\u003c\/p\u003e \u003cp\u003eThe Kidney 140\u003c\/p\u003e \u003cp\u003eBlood and Blood Vessels 142\u003c\/p\u003e \u003cp\u003eBlood 142\u003c\/p\u003e \u003cp\u003eBlood Vessels 143\u003c\/p\u003e \u003cp\u003eThe Heart 145\u003c\/p\u003e \u003cp\u003eThe Gut 146\u003c\/p\u003e \u003cp\u003eRegional Specification of the Endoderm 148\u003c\/p\u003e \u003cp\u003eThe Intestine 149\u003c\/p\u003e \u003cp\u003eThe Pancreas 150\u003c\/p\u003e \u003cp\u003eThe Liver 150\u003c\/p\u003e \u003cp\u003eFurther Reading 151\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Cell Differentiation and Growth 155\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eOrgans, Tissues and Cell Types 155\u003c\/p\u003e \u003cp\u003eEpithelia 156\u003c\/p\u003e \u003cp\u003eConnective Tissues 156\u003c\/p\u003e \u003cp\u003eCell Differentiation 158\u003c\/p\u003e \u003cp\u003eRegulation of Gene Activity 158\u003c\/p\u003e \u003cp\u003eLateral Inhibition 161\u003c\/p\u003e \u003cp\u003eAsymmetrical Cell Division 162\u003c\/p\u003e \u003cp\u003eNeurogenesis and Gliogenesis 164\u003c\/p\u003e \u003cp\u003eNeurons and Glia 164\u003c\/p\u003e \u003cp\u003eNeurogenesis 166\u003c\/p\u003e \u003cp\u003eGliogenesis 168\u003c\/p\u003e \u003cp\u003ePostnatal Cell Division 169\u003c\/p\u003e \u003cp\u003eAdult Neurogenesis 169\u003c\/p\u003e \u003cp\u003eNeurospheres 171\u003c\/p\u003e \u003cp\u003eSkeletal and Cardiac Muscle 171\u003c\/p\u003e \u003cp\u003eSkeletal Muscle 171\u003c\/p\u003e \u003cp\u003eDevelopment of Skeletal Muscle 172\u003c\/p\u003e \u003cp\u003eMuscle Satellite Cells 173\u003c\/p\u003e \u003cp\u003eCardiac Muscle 175\u003c\/p\u003e \u003cp\u003eEndodermal Tissues 176\u003c\/p\u003e \u003cp\u003eCell Differentiation in the Pancreas 176\u003c\/p\u003e \u003cp\u003eCell Differentiation in the Intestine 178\u003c\/p\u003e \u003cp\u003eCell Differentiation in the Liver 179\u003c\/p\u003e \u003cp\u003eHepatocytes and Cholangiocytes 180\u003c\/p\u003e \u003cp\u003eLiver Growth and Regeneration 181\u003c\/p\u003e \u003cp\u003eTransdifferentiation and Direct Reprogramming of Cell Type 183\u003c\/p\u003e \u003cp\u003eDifferentiation Protocols for Pluripotent Stem Cells 184\u003c\/p\u003e \u003cp\u003eFurther Reading 185\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Stem Cells in the Body 189\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eThe Intestinal Epithelium 189\u003c\/p\u003e \u003cp\u003eIntestinal Stem Cells 191\u003c\/p\u003e \u003cp\u003eIn Vitro Culture 193\u003c\/p\u003e \u003cp\u003eClonality of Intestinal Crypts 193\u003c\/p\u003e \u003cp\u003eThe Epidermis 195\u003c\/p\u003e \u003cp\u003eHair Follicles 197\u003c\/p\u003e \u003cp\u003eCornea and Limbus 199\u003c\/p\u003e \u003cp\u003eMammary Glands 200\u003c\/p\u003e \u003cp\u003eMammary Stem Cells 203\u003c\/p\u003e \u003cp\u003eThe Hematopoietic System 204\u003c\/p\u003e \u003cp\u003eAnalysis by Transplantation and in Vitro Culture 204\u003c\/p\u003e \u003cp\u003eHematopoiesis in the Steady State 207\u003c\/p\u003e \u003cp\u003eThe Hematopoietic Niche 209\u003c\/p\u003e \u003cp\u003eSpermatogenesis 211\u003c\/p\u003e \u003cp\u003eFurther Reading 213\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Regeneration, Wound Healing and Cancer 217\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003ePlanarian Regeneration 217\u003c\/p\u003e \u003cp\u003eNeoblasts 218\u003c\/p\u003e \u003cp\u003eAmphibian Limb Regeneration 220\u003c\/p\u003e \u003cp\u003eThe Regeneration Blastema 220\u003c\/p\u003e \u003cp\u003ePattern Formation in Regeneration 222\u003c\/p\u003e \u003cp\u003eMesenchymal Stem Cells 223\u003c\/p\u003e \u003cp\u003eMammalian Wound Healing 225\u003c\/p\u003e \u003cp\u003eSoft Tissue Wounds 225\u003c\/p\u003e \u003cp\u003eHealing of Bone Fractures 225\u003c\/p\u003e \u003cp\u003eSpinal Cord Injuries 227\u003c\/p\u003e \u003cp\u003eRegeneration and Repair 228\u003c\/p\u003e \u003cp\u003eCancer 228\u003c\/p\u003e \u003cp\u003eGenetic Heterogeneity of Cancer 230\u003c\/p\u003e \u003cp\u003eCancer Stem Cells 233\u003c\/p\u003e \u003cp\u003eFurther Reading 236\u003c\/p\u003e \u003cp\u003eIndex 239\u003c\/p\u003e   \u003cp\u003e\u003cb\u003e JONATHAN M. W. SLACK\u003c\/b\u003e is a developmental biologist and author of five books and over 200 scientific papers. He is an emeritus professor at the University of Bath, UK, as well as the University of Minnesota, USA, where he was Director of the Stem Cell Institute from 2007–2013.      \u003c\/p\u003e\u003cp\u003e\u003cb\u003e Introduces all of the essential cell biology and developmental biology background for the study of stem cells \u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e This book gives you all the important information you need to become a stem cell scientist. It covers the characterization of cells, genetic techniques for modifying cells and organisms, tissue culture technology, transplantation immunology, properties of pluripotent and tissue specific stem cells and, in particular, the relevant aspects of mammalian developmental biology. It dispels many misconceptions about stem cells—especially that they can be miracle cells that can cure all ills. The book puts emphasis on stem cell behavior in its biological context and on how to study it. Throughout, the approach is simple, direct, and logical, and evidence is given to support conclusions.   \u003c\/p\u003e\u003cp\u003e Stem cell biology has huge potential for advancing therapies for many distressing and recalcitrant diseases, and its potential will be realized most quickly when as many people as possible have a good grounding in the science of stem cells.   \u003c\/p\u003e\u003cul\u003e \u003cli\u003eContent focused on the basic science underpinning stem cell biology\u003c\/li\u003e \u003cli\u003eCovers techniques of studying cell properties and cell lineage in vivo and in vitro\u003c\/li\u003e \u003cli\u003eExplains the basics of embryonic development and cell differentiation, as well as the essential cell biology processes of signaling, gene expression, and cell division\u003c\/li\u003e \u003cli\u003eIncludes instructor resources such as further reading and figures for downloading\u003c\/li\u003e \u003cli\u003eOffers an online supplement summarizing current clinical applications of stem cells\u003c\/li\u003e \u003c\/ul\u003e \u003cbr\u003e  \u003cp\u003e Written by a prominent leader in the field, \u003ci\u003eThe Science of Stem Cells\u003c\/i\u003e is an ideal course book for advanced undergraduates or graduate students studying stem cell biology, regenerative medicine, tissue engineering, and other topics of science and biology.\u003c\/p\u003e","brand":"Wiley-Blackwell","offers":[{"title":"Default Title","offer_id":47990336323813,"sku":"NP9781119235156","price":112.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9781119235156.jpg?v=1761787408","url":"https:\/\/k12savings.com\/es\/products\/the-science-of-stem-cells-isbn-9781119235156","provider":"K12savings","version":"1.0","type":"link"}