{"product_id":"proteomics-in-practice-isbn-9783527319411","title":"Proteomics in Practice","description":"Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended. \u003cbr\u003e Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR.\u003cbr\u003e Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website. Preface\u003cbr\u003e Foreword\u003cbr\u003e Abbreviations\u003cbr\u003e Glossary of terms\u003cbr\u003e INTRODUCTION \u003cbr\u003e History\u003cbr\u003e Critical points\u003cbr\u003e Hypothesis driven approach vs. High Throughput \u003cbr\u003e Complementarities of workflows\u003cbr\u003e Concept of experimental planning\u003cbr\u003e Success stories, highlights of useful references\u003cbr\u003e PART I: PROTEOMICS TECHNOLOGY\u003cbr\u003e Electrophoresis\u003cbr\u003e Liquid Chromatography\u003cbr\u003e Mass spectrometry\u003cbr\u003e Bioinformatics tools \/ data mining\u003cbr\u003e Methods under development\u003cbr\u003e PART II. PRACTICAL MANUAL OF PROTEOME ANALYSIS\u003cbr\u003e Strategy where to start\u003cbr\u003e Gel-based workflow\u003cbr\u003e LC-based workflow\u003cbr\u003e Latest trends\u003cbr\u003e Validation with Western blotting\u003cbr\u003e Statistic evaluation\u003cbr\u003e PART III: TROUBLE SHOOTING\u003cbr\u003e Gel-based workflow\u003cbr\u003e LC based workflow\u003cbr\u003e References\u003cbr\u003e Index\u003cbr\u003e \"This book will be a valuable addition to any laboratory engaged in proteomics and is highly recommended.\" (\u003ci\u003eInternet Journal of Genomics and Proteomics\u003c\/i\u003e 2008) Reiner Westermeier gained his doctorate in 1981 at the Technical University of Munich, Germany. There he remained engaged in the development of new electrophoresis systems and applications until 1983, a period that also involved several international collaborations and lecturing tours.\u003cbr\u003e From 1984 to 1987 Reiner Westermeier was employed as an electrophoresis products and applications specialist at LKB Instrument GmbH thereafter until 1990 at Pharmacia Biotech. He founded ETC Elektrophorese-Technik in 1991 for the development of new electrophoresis methods, media, and equipment. From January 1996 to July 1997 he was manager of scientific support Europe at Pharmacia Biotech in Freiburg, Germany. He has been at Amersham Pharmacia Biotech, later GE Healthcare since July 1997, and its Senior Scientist for Proteomics since January 2002. \u003cbr\u003e Reiner Westermeier is the author of several publications and two books, both published by WILEY-VCH.\u003cbr\u003e Tom Naven and Hans-Rudolf Höpker are also working for GE Healthcare as proteomics scientists. A thoroughly revised edition of the successful practical guide to experimental methods in proteomics, which now includes DIGE technology and liquid chromatography.\u003cbr\u003e \u003cbr\u003e The first part, written in a textbook style, presents the various techniques and routes required in approaching proteomic analysis. Key methods explained are electrophoretic techniques including isoelectric focusing, SDS page, 2-D page and DIGE, and liquid chromatography techniques including ion exchange, affinity chromatography and reversed-phase HPLC.  Mass-spectrometric techniques such as MALDI, ESR and FT ICR are addressed as well as a section on proteome functionality.  The second part is a comprehensive, laboratory manual spanning the full range of methods from sample preparation to protein identification.  The third part is a unique troubleshooting guide, designed to answer many of the frequently asked questions regarding proteome analysis. The final section contains a thorough reference list to guide interested readers towards further information.\u003cbr\u003e \u003cbr\u003e Updates of protocols as well as animations illustrating crucial methodological steps are available on the Web at http:\/\/www.wiley-vch.de\/books\/info\/978-3-527-31941-1\/index.html\u003cbr\u003e \u003cbr\u003e","brand":"Wiley-Blackwell","offers":[{"title":"Default Title","offer_id":47989881012453,"sku":"NP9783527319411","price":130.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9783527319411.jpg?v=1761785776","url":"https:\/\/k12savings.com\/es\/products\/proteomics-in-practice-isbn-9783527319411","provider":"K12savings","version":"1.0","type":"link"}