{"product_id":"proteins-isbn-9780471498940","title":"Proteins","description":"\u003ci\u003eProteins: Structure and Function\u003c\/i\u003e is a comprehensive introduction to the study of proteins and their importance to modern biochemistry. Each chapter addresses the structure and function of proteins with a definitive theme designed to enhance student understanding. Opening with a brief historical overview of the subject the book moves on to discuss the ‘building blocks’ of proteins and their respective chemical and physical properties. Later chapters explore experimental and computational methods of comparing proteins, methods of protein purification and protein folding and stability. \u003cp\u003eThe latest developments in the field are included and key concepts introduced in a user-friendly way to ensure that students are able to grasp the essentials before moving on to more advanced study and analysis of proteins.\u003c\/p\u003e \u003cp\u003eAn invaluable resource for students of Biochemistry, Molecular Biology, Medicine and Chemistry providing a modern approach to the subject of Proteins.\u003c\/p\u003eEinen hervorragenden Uberblick uber Strukturen und Funktionen von Proteinen bietet Ihnen dieser Band, der insbesondere fur Studenten unterer Semester konzipiert ist. Der Autor arbeitet die Bedeutung der Proteine und der Proteinfaltung in der Biochemie anschaulich heraus und hilft Ihnen damit, eine solide Grundlage fur speziellere Studien zu schaffen. Vom zugehorigen Webserver konnen animierte Proteinstrukturen und Ubungsaufgaben mit Losungen abgerufen werden; au?erdem finden Sie dort Links zu Proteindatenbanken und anderen einschlagigen Webseiten.\u003cbr\u003e \u003cbr\u003e \u003cp\u003ePreface xi\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 An Introduction to protein structure and function 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eA brief and very selective historical perspective 1\u003c\/p\u003e \u003cp\u003eThe biological diversity of proteins 5\u003c\/p\u003e \u003cp\u003eProteins and the sequencing of the human and other genomes 9\u003c\/p\u003e \u003cp\u003eWhy study proteins? 9\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Amino acids: the building blocks of proteins 13\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eThe 20 amino acids found in proteins 13\u003c\/p\u003e \u003cp\u003eThe acid–base properties of amino acids 14\u003c\/p\u003e \u003cp\u003eStereochemical representations of amino acids 15\u003c\/p\u003e \u003cp\u003ePeptide bonds 16\u003c\/p\u003e \u003cp\u003eThe chemical and physical properties of amino acids 23\u003c\/p\u003e \u003cp\u003eDetection, identification and quantification of amino acids and proteins 32\u003c\/p\u003e \u003cp\u003eStereoisomerism 34\u003c\/p\u003e \u003cp\u003eNon-standard amino acids 35\u003c\/p\u003e \u003cp\u003eSummary 36\u003c\/p\u003e \u003cp\u003eProblems 37\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 The three-dimensional structure of proteins 39\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003ePrimary structure or sequence 39\u003c\/p\u003e \u003cp\u003eSecondary structure 39\u003c\/p\u003e \u003cp\u003eTertiary structure 50\u003c\/p\u003e \u003cp\u003eQuaternary structure 62\u003c\/p\u003e \u003cp\u003eThe globin family and the role of quaternary structure in modulating activity 66\u003c\/p\u003e \u003cp\u003eImmunoglobulins 74\u003c\/p\u003e \u003cp\u003eCyclic proteins 81\u003c\/p\u003e \u003cp\u003eSummary 81\u003c\/p\u003e \u003cp\u003eProblems 83\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 The structure and function of fibrous proteins 85\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eThe amino acid composition and organization of fibrous proteins 85\u003c\/p\u003e \u003cp\u003eKeratins 86\u003c\/p\u003e \u003cp\u003eFibroin 92\u003c\/p\u003e \u003cp\u003eCollagen 92\u003c\/p\u003e \u003cp\u003eSummary 102\u003c\/p\u003e \u003cp\u003eProblems 103\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 The structure and function of membrane proteins 105\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eThe molecular organization of membranes 105\u003c\/p\u003e \u003cp\u003eMembrane protein topology and function seen through organization of the erythrocyte membrane 110\u003c\/p\u003e \u003cp\u003eBacteriorhodopsin and the discovery of seven transmembrane helices 114\u003c\/p\u003e \u003cp\u003eThe structure of the bacterial reaction centre 123\u003c\/p\u003e \u003cp\u003eOxygenic photosynthesis 126\u003c\/p\u003e \u003cp\u003ePhotosystem I 126\u003c\/p\u003e \u003cp\u003eMembrane proteins based on transmembrane β barrels 128\u003c\/p\u003e \u003cp\u003eRespiratory complexes 132\u003c\/p\u003e \u003cp\u003eComplex III, the ubiquinol-cytochrome c oxidoreductase 132\u003c\/p\u003e \u003cp\u003eComplex IV or cytochrome oxidase 138\u003c\/p\u003e \u003cp\u003eThe structure of ATP synthetase 144\u003c\/p\u003e \u003cp\u003eATPase family 152\u003c\/p\u003e \u003cp\u003eSummary 156\u003c\/p\u003e \u003cp\u003eProblems 159\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 The diversity of proteins 161\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003ePrebiotic synthesis and the origins of proteins 161\u003c\/p\u003e \u003cp\u003eEvolutionary divergence of organisms and its relationship to protein structure and function 163\u003c\/p\u003e \u003cp\u003eProtein sequence analysis 165\u003c\/p\u003e \u003cp\u003eProtein databases 180\u003c\/p\u003e \u003cp\u003eGene fusion and duplication 181\u003c\/p\u003e \u003cp\u003eSecondary structure prediction 181\u003c\/p\u003e \u003cp\u003eGenomics and proteomics 183\u003c\/p\u003e \u003cp\u003eSummary 187\u003c\/p\u003e \u003cp\u003eProblems 187\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Enzyme kinetics, structure, function, and catalysis 189\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eEnzyme nomenclature 191\u003c\/p\u003e \u003cp\u003eEnzyme co-factors 192\u003c\/p\u003e \u003cp\u003eChemical kinetics 192\u003c\/p\u003e \u003cp\u003eThe transition state and the action of enzymes 195\u003c\/p\u003e \u003cp\u003eThe kinetics of enzyme action 197\u003c\/p\u003e \u003cp\u003eCatalytic mechanisms 202\u003c\/p\u003e \u003cp\u003eEnzyme structure 209\u003c\/p\u003e \u003cp\u003eLysozyme 209\u003c\/p\u003e \u003cp\u003eThe serine proteases 212\u003c\/p\u003e \u003cp\u003eTriose phosphate isomerase 215\u003c\/p\u003e \u003cp\u003eTyrosyl tRNA synthetase 218\u003c\/p\u003e \u003cp\u003eEcoRI restriction endonuclease 221\u003c\/p\u003e \u003cp\u003eEnzyme inhibition and regulation 224\u003c\/p\u003e \u003cp\u003eIrreversible inhibition of enzyme activity 227\u003c\/p\u003e \u003cp\u003eAllosteric regulation 231\u003c\/p\u003e \u003cp\u003eCovalent modification 237\u003c\/p\u003e \u003cp\u003eIsoenzymes or isozymes 241\u003c\/p\u003e \u003cp\u003eSummary 242\u003c\/p\u003e \u003cp\u003eProblems 244\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Protein synthesis, processing and turnover 247\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eCell cycle 247\u003c\/p\u003e \u003cp\u003eThe structure of Cdk and its role in the cell cycle 250\u003c\/p\u003e \u003cp\u003eCdk–cyclin complex regulation 252\u003c\/p\u003e \u003cp\u003eDNA replication 253\u003c\/p\u003e \u003cp\u003eTranscription 254\u003c\/p\u003e \u003cp\u003eEukaryotic transcription factors: variation on a ‘basic’ theme 261\u003c\/p\u003e \u003cp\u003eThe spliceosome and its role in transcription 265\u003c\/p\u003e \u003cp\u003eTranslation 266\u003c\/p\u003e \u003cp\u003eTransfer RNA (tRNA) 267\u003c\/p\u003e \u003cp\u003eThe composition of prokaryotic and eukaryotic ribosomes 269\u003c\/p\u003e \u003cp\u003eA structural basis for protein synthesis 272\u003c\/p\u003e \u003cp\u003eAn outline of protein synthesis 273\u003c\/p\u003e \u003cp\u003eAntibiotics provide insight into protein synthesis 278\u003c\/p\u003e \u003cp\u003eAffinity labelling and RNA ‘footprinting’ 279\u003c\/p\u003e \u003cp\u003eStructural studies of the ribosome 279\u003c\/p\u003e \u003cp\u003ePost-translational modification of proteins 287\u003c\/p\u003e \u003cp\u003eProtein sorting or targeting 293\u003c\/p\u003e \u003cp\u003eThe nuclear pore assembly 302\u003c\/p\u003e \u003cp\u003eProtein turnover 303\u003c\/p\u003e \u003cp\u003eApoptosis 310\u003c\/p\u003e \u003cp\u003eSummary 310\u003c\/p\u003e \u003cp\u003eProblems 312\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Protein expression, purification and characterization 313\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eThe isolation and characterization of proteins 313\u003c\/p\u003e \u003cp\u003eRecombinant DNA technology and protein expression 313\u003c\/p\u003e \u003cp\u003ePurification of proteins 318\u003c\/p\u003e \u003cp\u003eCentrifugation 320\u003c\/p\u003e \u003cp\u003eSolubility and ‘salting out’ and ‘salting in’ 323\u003c\/p\u003e \u003cp\u003eChromatography 326\u003c\/p\u003e \u003cp\u003eDialysis and ultrafiltration 333\u003c\/p\u003e \u003cp\u003ePolyacrylamide gel electrophoresis 333\u003c\/p\u003e \u003cp\u003eMass spectrometry 340\u003c\/p\u003e \u003cp\u003eHow to purify a protein? 342\u003c\/p\u003e \u003cp\u003eSummary 344\u003c\/p\u003e \u003cp\u003eProblems 345\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Physical methods of determining the three-dimensional structure of proteins 347\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 347\u003c\/p\u003e \u003cp\u003eThe use of electromagnetic radiation 348\u003c\/p\u003e \u003cp\u003eX-ray crystallography 349\u003c\/p\u003e \u003cp\u003eNuclear magnetic resonance spectroscopy 360\u003c\/p\u003e \u003cp\u003eCryoelectron microscopy 375\u003c\/p\u003e \u003cp\u003eNeutron diffraction 379\u003c\/p\u003e \u003cp\u003eOptical spectroscopic techniques 379\u003c\/p\u003e \u003cp\u003eVibrational spectroscopy 387\u003c\/p\u003e \u003cp\u003eRaman spectroscopy 389\u003c\/p\u003e \u003cp\u003eESR and ENDOR 390\u003c\/p\u003e \u003cp\u003eSummary 392\u003c\/p\u003e \u003cp\u003eProblems 393\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Protein folding in vivo and in vitro 395\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 395\u003c\/p\u003e \u003cp\u003eFactors determining the protein fold 395\u003c\/p\u003e \u003cp\u003eFactors governing protein stability 403\u003c\/p\u003e \u003cp\u003eFolding problem and Levinthal’s paradox 403\u003c\/p\u003e \u003cp\u003eModels of protein folding 408\u003c\/p\u003e \u003cp\u003eAmide exchange and measurement of protein folding 411\u003c\/p\u003e \u003cp\u003eKinetic barriers to refolding 412\u003c\/p\u003e \u003cp\u003eIn vivo protein folding 415\u003c\/p\u003e \u003cp\u003eMembrane protein folding 422\u003c\/p\u003e \u003cp\u003eProtein misfolding and the disease state 426\u003c\/p\u003e \u003cp\u003eSummary 435\u003c\/p\u003e \u003cp\u003eProblems 437\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Protein structure and a molecular approach to medicine 439\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 439\u003c\/p\u003e \u003cp\u003eSickle cell anaemia 441\u003c\/p\u003e \u003cp\u003eViruses and their impact on health as seen through structure and function 442\u003c\/p\u003e \u003cp\u003eHIV and AIDS 443\u003c\/p\u003e \u003cp\u003eThe influenza virus 457\u003c\/p\u003e \u003cp\u003ep53 and its role in cancer 470\u003c\/p\u003e \u003cp\u003eEmphysema and α1-antitrypsin 475\u003c\/p\u003e \u003cp\u003eSummary 478\u003c\/p\u003e \u003cp\u003eProblems 479\u003c\/p\u003e \u003cp\u003eEpilogue 481\u003c\/p\u003e \u003cp\u003eGlossary 483\u003c\/p\u003e \u003cp\u003eAppendices 491\u003c\/p\u003e \u003cp\u003eBibliography 495\u003c\/p\u003e \u003cp\u003eReferences 499\u003c\/p\u003e \u003cp\u003eIndex 511\u003c\/p\u003e  \"…an invaluable resource…students of biochemistry, chemistry, genetics, structural biology, as well as medical and dental students will greatly benefit from reading this text…\" (\u003ci\u003eAnnals of Biomedical Engineering\u003c\/i\u003e, December 2005)  \u003cp\u003e\"…a worthwhile investment for the beginning student or for the advanced scientist seeking a better grasp of protein chemistry…\" (\u003ci\u003eClinical Chemistry\u003c\/i\u003e, November 2005)\u003c\/p\u003e \u003cp\u003e\"An outstanding resource for introductory protein chemistry courses and a solid reference for those interested in proteins.\" (\u003ci\u003eCHOIC\u003c\/i\u003eE, November 2005)\u003c\/p\u003e \u003cp\u003e\" … the distillation of years of lecturing and undergraduate feedback.\" (\u003ci\u003eJournal of Biological Education, Spring 2006: Vol.40; 2)\u003c\/i\u003e\u003c\/p\u003e \u003cb\u003eDavid Whitford\u003c\/b\u003e, Queen Mary \u0026amp; Westfield College, London, UK.  \u003cb\u003eProtein Structure and Function\u003c\/b\u003e is a comprehensive introduction to the study of proteins and their importance to modern biochemistry. Each chapter addresses the structure and function of proteins with a definitive theme designed to enhance student understanding. Opening with a brief historical overview of the subject the book moves on to discuss the ‘building blocks’ of proteins and their respective chemical and physical properties. Later chapters explore experimental and computational methods of comparing proteins, methods of protein purification and protein folding and stability.  \u003cp\u003eThe latest developments in the field are included and key concepts introduced in a user-friendly way to ensure that students are able to grasp the essentials before moving on to more advanced study and analysis of proteins.\u003c\/p\u003e \u003cp\u003eAn invaluable resource for students of Biochemistry, Molecular Biology, Medicine and Chemistry providing a modern approach to the subject of Proteins.\u003c\/p\u003e \u003cul\u003e \u003cli\u003eAn accessible introduction to the principles of protein structure and function.\u003c\/li\u003e \u003cli\u003eBeautifully illustrated throughout in full colour.\u003c\/li\u003e \u003cli\u003eIncludes end of chapter problems, references to further reading and a full glossary of terms.\u003c\/li\u003e \u003cli\u003eAssociated website including web-based tutorials, problems and additional content.\u003c\/li\u003e \u003c\/ul\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47989880881381,"sku":"NP9780471498940","price":105.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9780471498940.jpg?v=1761785774","url":"https:\/\/k12savings.com\/es\/products\/proteins-isbn-9780471498940","provider":"K12savings","version":"1.0","type":"link"}