{"product_id":"practical-flow-cytometry-in-haematology-diagnosis-isbn-9780470671207","title":"Practical Flow Cytometry in Haematology Diagnosis","description":"\u003cp\u003eThe accurate diagnosis of haematologic malignancies is a complex and challenging task. It routinely involves morphologic, molecular, cytogenetic and flow cytometric expertise. To determine what treatment protocol will be followed, it is vital to integrate, interpret and report these results accurately.\u003c\/p\u003e \u003cp\u003eFlow cytometry is key in this diagnostic pathway.\u003c\/p\u003e \u003cp\u003eThis book guides the reader as to how flow cytometry results should be interpreted and applied to optimize patient care. At the core of this text is an appreciation of clinical, morphological and immunophenotypic correlation and the importance of constant liaison and discussion between the medical and scientific teams.\u003c\/p\u003e \u003cp\u003eThe authors present a logical and practical approach to the diagnosis of blood disorders (both neoplastic and reactive) and evaluate the diagnostic applications of flow cytometry.\u003c\/p\u003e \u003cp\u003e\u003ci\u003ePractical Flow Cytometry in Haematology Diagnosis\u003c\/i\u003e provides:\u003c\/p\u003e \u003cul\u003e \u003cli\u003eA clinical reference source on all aspects of flow cytometry, covering both malignant and benign conditionsCarefully chosen real-life cases in each chapter, complemented by high quality morphological images\u003c\/li\u003e \u003cli\u003eHelp in making a diagnosis, together with an understanding of the limitations of the technique and the potential pitfalls\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eAll those who instigate, perform, interpret or act upon flow cytometry patient material will find this book an invaluable guide.\u003c\/p\u003e \u003cp\u003ePreface, vii\u003c\/p\u003e \u003cp\u003eAcknowledgements, viii\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Introduction, 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eReferences, 2\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Principles of Flow Cytometry, 3\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction, 3\u003c\/p\u003e \u003cp\u003eSample preparation, 3\u003c\/p\u003e \u003cp\u003eThe flow cytometer, 4\u003c\/p\u003e \u003cp\u003eThe fluidic system, 4\u003c\/p\u003e \u003cp\u003eThe optical system, 5\u003c\/p\u003e \u003cp\u003eThe electronic system, 9\u003c\/p\u003e \u003cp\u003eData display, 9\u003c\/p\u003e \u003cp\u003eAcknowledgements, 18\u003c\/p\u003e \u003cp\u003eReferences, 19\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Limitations, 20\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction, 20\u003c\/p\u003e \u003cp\u003eClinical context issues, 20\u003c\/p\u003e \u003cp\u003eSampling issues, 20\u003c\/p\u003e \u003cp\u003eTechnical issues, 25\u003c\/p\u003e \u003cp\u003eInterpretation issues, 28\u003c\/p\u003e \u003cp\u003eConclusion, 29\u003c\/p\u003e \u003cp\u003eReferences, 29\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Normal Blood and Bone Marrow Populations, 31\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eNormal stem and precursor cell populations, 31\u003c\/p\u003e \u003cp\u003eReferences, 41\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Acute Leukaemia, 43\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction, 43\u003c\/p\u003e \u003cp\u003eIdentification of neoplastic precursor cells, 45\u003c\/p\u003e \u003cp\u003eLineage assignment of acute leukaemia, 52\u003c\/p\u003e \u003cp\u003eAcute myeloid leukaemia, 54\u003c\/p\u003e \u003cp\u003ePrecursor lymphoid neoplasms (acute lymphoblastic leukaemia\/lymphoma), 77\u003c\/p\u003e \u003cp\u003eExamination of cerebro-spinal fluid and serous effusions, 88\u003c\/p\u003e \u003cp\u003eIdentification of genetic abnormalities by flow, 92\u003c\/p\u003e \u003cp\u003eConclusion, 93\u003c\/p\u003e \u003cp\u003eReferences, 93\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Chronic Lymphoid Leukaemias and Exfoliating Lymphoma, 100\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eNormal peripheral blood lymphoid populations, 100\u003c\/p\u003e \u003cp\u003eIdentification of clonal lymphoid populations, 101\u003c\/p\u003e \u003cp\u003eIdentification of clonal B-cell disorders, 101\u003c\/p\u003e \u003cp\u003eCD5 positive B-cell lymphoproliferative disorders, 102\u003c\/p\u003e \u003cp\u003eCD10 positive B-cell lymphoproliferative disorders, 116\u003c\/p\u003e \u003cp\u003eB-cell disorders identified using an extended B-cell panel, 121\u003c\/p\u003e \u003cp\u003eIdentification of clonal T-cell disorders, 125\u003c\/p\u003e \u003cp\u003eCD4 positive T-cell disorders, 128\u003c\/p\u003e \u003cp\u003eCD8 positive T-cell disorders, 136\u003c\/p\u003e \u003cp\u003eCD4\/CD8 positive T-cell disorders, 141\u003c\/p\u003e \u003cp\u003eCD4\/8 negative disorders, 141\u003c\/p\u003e \u003cp\u003eFlow cytometric assessment of serous effusions, 146\u003c\/p\u003e \u003cp\u003eFlow cytometric assessment of cerebrospinal fluid, 146\u003c\/p\u003e \u003cp\u003eSummary, 149\u003c\/p\u003e \u003cp\u003eReferences, 149\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Myelodysplastic Syndromes and Myeloproliferative Neoplasms, 152\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction, 152\u003c\/p\u003e \u003cp\u003eMyelodysplastic syndromes, 152\u003c\/p\u003e \u003cp\u003eMyelodysplastic\/myeloproliferative neoplasms, 163\u003c\/p\u003e \u003cp\u003eChronic myeloid leukaemia, 165\u003c\/p\u003e \u003cp\u003eThe BCR-ABL negative myeloproliferative neoplasms, 166\u003c\/p\u003e \u003cp\u003eMastocytosis, 167\u003c\/p\u003e \u003cp\u003eSummary, 170\u003c\/p\u003e \u003cp\u003eReferences, 170\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Disorders of Plasma Cells, 173\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003ePlasma cell disorders, 173\u003c\/p\u003e \u003cp\u003eCurrent diagnostic criteria, 173\u003c\/p\u003e \u003cp\u003ePlasma cell morphology, 175\u003c\/p\u003e \u003cp\u003eThe applications of immunophenotyping to plasma cell disorders, 178\u003c\/p\u003e \u003cp\u003eReferences, 183\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Minimal Residual Disease, 184\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction, 184\u003c\/p\u003e \u003cp\u003eMinimal residual disease analysis in the management of acute lymphoblastic leukaemia, 187\u003c\/p\u003e \u003cp\u003eConclusions, 199\u003c\/p\u003e \u003cp\u003eAcknowledgements, 200\u003c\/p\u003e \u003cp\u003eReferences, 200\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Red Cells, Leucocytes and Platelets, 202\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eParoxysmal nocturnal haemoglobinuria, 202\u003c\/p\u003e \u003cp\u003eRed cell membrane disorders, 208\u003c\/p\u003e \u003cp\u003eFoetal maternal haemorrhage, 211\u003c\/p\u003e \u003cp\u003eLymphocyte subset analysis\u003c\/p\u003e \u003cp\u003eand immunodeficiency, 214\u003c\/p\u003e \u003cp\u003eHaemopoietic stem cell enumeration, 214\u003c\/p\u003e \u003cp\u003eGranulocyte disorders, 215\u003c\/p\u003e \u003cp\u003ePlatelet disorders, 219\u003c\/p\u003e \u003cp\u003eReferences, 221\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Reactive and Non-neoplastic Phenomena, 225\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003ePeripheral blood, 225\u003c\/p\u003e \u003cp\u003eBone marrow, 231\u003c\/p\u003e \u003cp\u003ePleural and pericardial fluids, 236\u003c\/p\u003e \u003cp\u003eBronchoalveolar lavage specimens, 238\u003c\/p\u003e \u003cp\u003eCerebrospinal fluid, 238\u003c\/p\u003e \u003cp\u003eReferences, 240\u003c\/p\u003e \u003cp\u003eIndex, 243\u003c\/p\u003e  \u003cp\u003e“I would particularly recommend this book to haematology trainees, as an introduction to, but not as a replacement for, a period spent in an immunophenotyping laboratory.”  (\u003ci\u003eBritish Journal of Haematology\u003c\/i\u003e, 14 November 2014)\u003c\/p\u003e \u003cp\u003e“There is no doubt that this book will find its way firmly onto the bookshelf in every laboratory, and into the work bags of many trainees.”  (\u003ci\u003eHaem Trainee\u003c\/i\u003e, 1 January 2014)\u003c\/p\u003e \u003cp\u003e“This is a concise and very practical guide to the use of flow cytometry in hematological and hematopathological diagnosis. While not as detailed in immunophenotyping intricacies as other books of its kind, it shines with its practical approach and expert guidance applied to current clinical practice.”  (\u003ci\u003eDoody’s\u003c\/i\u003e, 19 July 2013)\u003c\/p\u003e \u003cp\u003e\u003cb\u003eDr Mike Leach\u003c\/b\u003e - Department of Haematology, Gartnavel General Hospital, Glasgow, UK\u003c\/p\u003e \u003cp\u003e\u003cb\u003eDr Mark Drummond\u003c\/b\u003e - Haematology Department, Beatson West of Scotland Cancer Centre, Glasgow, UK\u003c\/p\u003e \u003cp\u003e\u003cb\u003eDr Allyson Doig\u003c\/b\u003e - Department of Haematology, Gartnavel Hospital, Glasgow, UK\u003c\/p\u003e","brand":"Wiley-Blackwell","offers":[{"title":"Default Title","offer_id":47989831139557,"sku":"NP9780470671207","price":171.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9780470671207.jpg?v=1761785608","url":"https:\/\/k12savings.com\/es\/products\/practical-flow-cytometry-in-haematology-diagnosis-isbn-9780470671207","provider":"K12savings","version":"1.0","type":"link"}