{"product_id":"neural-stem-cell-assays-isbn-9781118308257","title":"Neural Stem Cell Assays","description":"Neural stem cells offer a valuable model system for delineating the cellular and developmental processes in normal and diseased states of the central nervous system. In particular, neural stem cells have huge potential in regenerative medicine, owing to their expansion capability in culture and the ability to differentiate into multiple sub-neural lineages. \u003cp\u003e\u003ci\u003eNeural Stem Cell Assays\u003c\/i\u003e provides a detailed and comprehensive review of the basic methods for neural stem cell cultures. Including an overview of progress in the field over the past decade, \u003ci\u003eNeural Stem Cell Assays\u003c\/i\u003e is a one-stop reference for consistent methods and reliable tools that span the entire assay work flow, from isolation or generation of neural stem cells to characterization, manipulation and final application of neural stem cells in disease paradigms such as Parkinson's disease, multiple sclerosis and amyotrophic lateral sclerosis.\u003c\/p\u003e \u003cp\u003eAn excellent source of information for academic, pharmaceutical and biotechnology researchers who are new to the neural stem cell field, \u003ci\u003eNeural Stem Cell Assays\u003c\/i\u003e is an invaluable to experienced users who wish to integrate newly developed tools and technologies into their workflow. The book also covers important course material for students at the undergraduate and graduate level who are learning the basics of neural stem cell cultures, and differentiation to sub-neural lineages.\u003c\/p\u003e  \u003cp\u003eContributors xv\u003c\/p\u003e \u003cp\u003ePreface xix\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Neural Differentiation of Pluripotent Stem Cells 1\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eMohan C. Vemuri\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e1.1 Introduction 1\u003c\/p\u003e \u003cp\u003e1.2 NSC Derivation from Rosette Formation of Embryoid Bodies 1\u003c\/p\u003e \u003cp\u003e1.3 Rosette Free NSC in a Monolayer Culture 2\u003c\/p\u003e \u003cp\u003e1.4 Pathways Involved in Neural Tube and Neural Crest Lineages 2\u003c\/p\u003e \u003cp\u003e1.5 Differentiation and Gene Expression in Human Brain Development 3\u003c\/p\u003e \u003cp\u003e1.6 Conclusions 6\u003c\/p\u003e \u003cp\u003eReferences 6\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 The History and Design of Assays for the Identification and Characterization of Neural Stem Cells 9\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eSharon A. Louis and Brent A. Reynolds\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2.1 Introduction 9\u003c\/p\u003e \u003cp\u003e2.2 Materials 12\u003c\/p\u003e \u003cp\u003e2.3 Methods 14\u003c\/p\u003e \u003cp\u003e2.4 Notes 21\u003c\/p\u003e \u003cp\u003eAcknowledgments 23\u003c\/p\u003e \u003cp\u003eReferences 23\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Culture of Human Neural Stem Cells 25\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eSoojung Shin, Yiping Yan and Mohan C Vemuri\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introduction 25\u003c\/p\u003e \u003cp\u003e3.2 Materials 25\u003c\/p\u003e \u003cp\u003e3.3 Methods 30\u003c\/p\u003e \u003cp\u003e3.4 Notes 33\u003c\/p\u003e \u003cp\u003e3.5 Expected Results 33\u003c\/p\u003e \u003cp\u003eReferences 34\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 GMP4-Compatible Production and Expansion of Human Neural Stem Cells 35\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eDavid J. Brick, Alexander E. Stover, Hubert E. Nethercott, Maria G. Banuelos and Philip H. Schwartz\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e4.1 Introduction 35\u003c\/p\u003e \u003cp\u003e4.2 Materials 36\u003c\/p\u003e \u003cp\u003e4.3 Methods 40\u003c\/p\u003e \u003cp\u003e4.4 Notes 50\u003c\/p\u003e \u003cp\u003eAcknowledgments 52\u003c\/p\u003e \u003cp\u003eReferences 52\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Primary Rat Neural Cultures 55\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eNavjot Kaur\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction 55\u003c\/p\u003e \u003cp\u003e5.2 Materials 55\u003c\/p\u003e \u003cp\u003e5.3 Methods 57\u003c\/p\u003e \u003cp\u003e5.4 Notes 59\u003c\/p\u003e \u003cp\u003eReferences 59\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Cryopreservation of Human Neural Stem and Progenitor Cells 61\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eProfico Daniela Celeste, Sgaravizzi Giada, Projetti Pensi Massimo, Muzi Gianmarco, Ricciolini Claudia, Gelati Maurizio and Vescovi Angelo Luigi\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction 61\u003c\/p\u003e \u003cp\u003e6.2 Materials 62\u003c\/p\u003e \u003cp\u003e6.3 Methods 62\u003c\/p\u003e \u003cp\u003e6.4 Notes 65\u003c\/p\u003e \u003cp\u003eReferences 65\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Cryopreservation and Recovery of Primary Rat Neural Cells 67\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eRhonda Newman and Navjot Kaur\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction 67\u003c\/p\u003e \u003cp\u003e7.2 Materials 67\u003c\/p\u003e \u003cp\u003e7.3 Methods 68\u003c\/p\u003e \u003cp\u003e7.4 Notes 70\u003c\/p\u003e \u003cp\u003eReferences 70\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Efficient Induction and Scale up Generation of Neural Stem Cells from Human Pluripotent Stem Cells 71\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eYiping Yan, Soojung Shin and Mohan C. Vemuri\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction 71\u003c\/p\u003e \u003cp\u003e8.2 Materials 71\u003c\/p\u003e \u003cp\u003e8.3 Methods 73\u003c\/p\u003e \u003cp\u003e8.4 Notes 78\u003c\/p\u003e \u003cp\u003eReferences 79\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Directed Differentiation of Human NSC\/NPC into Dopaminergic Neurons 81\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eAndrzej Swistowski and Xianmin Zeng\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction 81\u003c\/p\u003e \u003cp\u003e9.2 Materials 82\u003c\/p\u003e \u003cp\u003e9.3 Methods 83\u003c\/p\u003e \u003cp\u003e9.4 Notes 88\u003c\/p\u003e \u003cp\u003eReferences 88\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 In vitro Differentiation of Pluripotent Stem Cells towards either Forebrain GABAergic or Midbrain Dopaminergic Neurons 91\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eBrigham J. Hartley, Bradley Watmuff, Cameron P.J. Hunt, John M. Haynes and Colin W. Pouton\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e10.1 Introduction 91\u003c\/p\u003e \u003cp\u003e10.2 Materials 91\u003c\/p\u003e \u003cp\u003e10.3 Methods 94\u003c\/p\u003e \u003cp\u003e10.4 Notes 97\u003c\/p\u003e \u003cp\u003e10.5 Typical Results 97\u003c\/p\u003e \u003cp\u003eReferences 98\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Generation of Astrocytes from Human Pluripotent Stem Cells using a Defined System 101\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eAtossa Shaltouki and Xianmin Zeng\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e11.1 Introduction 101\u003c\/p\u003e \u003cp\u003e11.2 Materials and Methods 101\u003c\/p\u003e \u003cp\u003e11.3 Notes 108\u003c\/p\u003e \u003cp\u003eReferences 108\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Directed Differentiation of Human PSC into Oligodendrocytes 111\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eAnu Hyysalo and Susanna Narkilahti\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction 111\u003c\/p\u003e \u003cp\u003e12.2 Materials 111\u003c\/p\u003e \u003cp\u003e12.3 Methods 113\u003c\/p\u003e \u003cp\u003e12.4 Notes 116\u003c\/p\u003e \u003cp\u003eAcknowledgments 117\u003c\/p\u003e \u003cp\u003eReferences 117\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Directed Differentiation towards Human Neural Retinal Cells 119\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eDeepak A. Lamba\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e13.1 Introduction 119\u003c\/p\u003e \u003cp\u003e13.2 Materials 120\u003c\/p\u003e \u003cp\u003e13.3 Methods 121\u003c\/p\u003e \u003cp\u003eReferences 126\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 Directed Differentiation of Photoreceptors and Retinal Pigment Epithelium from Adult Mouse Retinal Stem Cells 129\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eBrian G. Ballios, Valeria Marigo and Derek van der Kooy\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e14.1 Introduction 129\u003c\/p\u003e \u003cp\u003e14.2 Materials 130\u003c\/p\u003e \u003cp\u003e14.3 Methods 131\u003c\/p\u003e \u003cp\u003e14.4 Notes 134\u003c\/p\u003e \u003cp\u003eAcknowledgments 134\u003c\/p\u003e \u003cp\u003eReferences 134\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 Induction of Schwann Cells from Rat Bone Marrow Mesenchymal Stem Cells 137\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eShohei Wakao, Masaaki Kitada and Mari Dezawa\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e15.1 Introduction 137\u003c\/p\u003e \u003cp\u003e15.2 Materials 138\u003c\/p\u003e \u003cp\u003e15.3 Method 141\u003c\/p\u003e \u003cp\u003e15.4 Notes 149\u003c\/p\u003e \u003cp\u003eReferences 151\u003c\/p\u003e \u003cp\u003e\u003cb\u003e16 Neural Cell Viability Assays 155\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eBonnie J. Hanson and Michael K. Hancock\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e16.1 Introduction 155\u003c\/p\u003e \u003cp\u003e16.2 Materials 157\u003c\/p\u003e \u003cp\u003e16.3 Methods 158\u003c\/p\u003e \u003cp\u003e16.4 Notes 165\u003c\/p\u003e \u003cp\u003eReferences 165\u003c\/p\u003e \u003cp\u003e\u003cb\u003e17 Genetic Modification of Pluripotent Stem Cell Derived Human Neural Progenitor Cells 167\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eJamie M. Chilton and Steven L. Stice\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e17.1 Introduction 167\u003c\/p\u003e \u003cp\u003e17.2 Materials 168\u003c\/p\u003e \u003cp\u003e17.3 Methods 170\u003c\/p\u003e \u003cp\u003e17.4 Notes 176\u003c\/p\u003e \u003cp\u003eAcknowledgments 178\u003c\/p\u003e \u003cp\u003eReferences 178\u003c\/p\u003e \u003cp\u003e\u003cb\u003e18 Electrophysiology of Neurons 179\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eChristine M. Emnett and Steven Mennerick\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e18.1 Introduction 179\u003c\/p\u003e \u003cp\u003e18.2 Materials 180\u003c\/p\u003e \u003cp\u003e18.3 Methods 181\u003c\/p\u003e \u003cp\u003e18.4 Notes 185\u003c\/p\u003e \u003cp\u003eReferences 187\u003c\/p\u003e \u003cp\u003e\u003cb\u003e19 Induction and Transplantation of Neural Stem Cells from Human Pluripotent Stem Cells in Experimental Models (Rat) of Parkinson’s disease 189\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eMakoto Motono, Asuka Morizane and Jun Takahashi\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e19.1 Introduction 189\u003c\/p\u003e \u003cp\u003e19.2 Materials 189\u003c\/p\u003e \u003cp\u003e19.3 Methods 191\u003c\/p\u003e \u003cp\u003e19.4 Notes 194\u003c\/p\u003e \u003cp\u003eAcknowledgments 194\u003c\/p\u003e \u003cp\u003eReferences 194\u003c\/p\u003e \u003cp\u003e\u003cb\u003e20 Transplantation of Human Neural Stem Cells and Progenitors in Animal Models of Disease 197\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eJoseph F. Bonner and Itzhak Fischer\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e20.1 Introduction 197\u003c\/p\u003e \u003cp\u003e20.2 General Principles of Effective Transplantation 198\u003c\/p\u003e \u003cp\u003e20.3 Different Methods of Transplantation 204\u003c\/p\u003e \u003cp\u003e20.4 Materials and Method 205\u003c\/p\u003e \u003cp\u003e20.5 Conclusions 211\u003c\/p\u003e \u003cp\u003e20.6 Notes 212\u003c\/p\u003e \u003cp\u003eAcknowledgments 212\u003c\/p\u003e \u003cp\u003eReferences 212\u003c\/p\u003e \u003cp\u003e\u003cb\u003e21 Morphometric Assays for Neural Differentiation and Synaptic Development of Human iPSC-Derived NSC 215\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eBarbara Calabrese, Leif Dehmelt and Shelley Halpain\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e21.1 Introduction 215\u003c\/p\u003e \u003cp\u003e21.2 Materials 216\u003c\/p\u003e \u003cp\u003e21.3 Method 218\u003c\/p\u003e \u003cp\u003e21.4 Notes 222\u003c\/p\u003e \u003cp\u003eAcknowledgments 223\u003c\/p\u003e \u003cp\u003eReferences 223\u003c\/p\u003e \u003cp\u003e\u003cb\u003e22 BacMam Transduction of Neural Stem Cells 225\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eRene Quintanilla and Uma Lakshmipathy\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e22.1 Introduction 225\u003c\/p\u003e \u003cp\u003e22.2 Materials 225\u003c\/p\u003e \u003cp\u003e22.3 Methods 227\u003c\/p\u003e \u003cp\u003e22.4 Notes 228\u003c\/p\u003e \u003cp\u003eAcknowledgments 229\u003c\/p\u003e \u003cp\u003eReferences 229\u003c\/p\u003e \u003cp\u003e\u003cb\u003e23 The Proximity Ligation Assay: A High Throughput Technique for Protein Analysis in Neuroscience 231\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eMichela Zaltieri, PierFranco Spano, Cristina Missale and Arianna Bellucci\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e23.1 Introduction 231\u003c\/p\u003e \u003cp\u003e23.2 Materials 232\u003c\/p\u003e \u003cp\u003e23.3 Methods 232\u003c\/p\u003e \u003cp\u003e23.4 Notes 238\u003c\/p\u003e \u003cp\u003e23.5 Troubleshooting 238\u003c\/p\u003e \u003cp\u003eAcknowledgments 240\u003c\/p\u003e \u003cp\u003eReferences 240\u003c\/p\u003e \u003cp\u003e\u003cb\u003e24 Quantification of Micro-RNA Expression in Human Neural Stem Cells using TaqMan Assays 241\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eXavier Nissan and Alexandra Benchoua\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e24.1 Introduction 241\u003c\/p\u003e \u003cp\u003e24.2 Materials 242\u003c\/p\u003e \u003cp\u003e24.3 Methods 244\u003c\/p\u003e \u003cp\u003e24.4 Notes 248\u003c\/p\u003e \u003cp\u003eReferences 249\u003c\/p\u003e \u003cp\u003e\u003cb\u003e25 Genetic Reporter Cell Lines: Tools for Stem Cell Biology and Drug Discovery 251\u003c\/b\u003e\u003cbr\u003e \u003ci\u003eCameron P.J. Hunt, Bradley Watmuff, Brigham J. Hartley, Colin W. Pouton and John M. Haynes\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e25.1 Pluripotent Stem Cell Reporter Lines 251\u003c\/p\u003e \u003cp\u003e25.2 Choice of PSC Genetic Reporter Systems 251\u003c\/p\u003e \u003cp\u003e25.3 Reporter Systems 252\u003c\/p\u003e \u003cp\u003e25.4 Multiple Reporter Lines 254\u003c\/p\u003e \u003cp\u003e25.5 Use of Genetic Reporter Escs to Study Neural Differentiation 255\u003c\/p\u003e \u003cp\u003eReferences 260\u003c\/p\u003e \u003cp\u003eIndex 261\u003c\/p\u003e  \u003cp\u003e\u003cstrong\u003eNavjot Kaur\u003c\/strong\u003e, Ph.D, is Staff Scientist in the Life Technologies division of the Invitrogen Corporation. His research experience covers a wide range of cell biology, protein biochemistry and molecular biology concepts, with special expertise in cell culture and cryopreservation media development, cell-based assays, primary cells, and stable cell lines. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eMohan C. Vemuri\u003c\/strong\u003e, Ph.D.,MBA is director of stem cell research and development in the Life Technologies division of Invitrogen \u0026amp; Applied Biosystems. The author of designs of peer-reviewed articles in the field, Dr. Vemuri is also a co-editor of three books on stem cells and stem cell cultures.\u003c\/p\u003e","brand":"Wiley-Blackwell","offers":[{"title":"Default Title","offer_id":47989682372837,"sku":"NP9781118308257","price":173.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9781118308257.jpg?v=1761785087","url":"https:\/\/k12savings.com\/es\/products\/neural-stem-cell-assays-isbn-9781118308257","provider":"K12savings","version":"1.0","type":"link"}