Motion Analysis of Living Cells

Most active cellular functions involve motion. From such subtle subcellular actions as the sorting and targeting of vesicles and organelles leading to the release of hormones to the complex rearrangements of a sphere of embryonic cells forming the body plan of a complete organism, motility of cells is exhibited along a wide range of observable behaviors. Despite this phenomenon, however, the views of modern biology historically have been based on static images and models constructed from biochemical evidence.

Motion Analysis of Living Cells is the first volume to compile the latest research by prominent specialists in molecular and cell biology, presenting newly developed techniques for studying cellular motions and critical analyses of the information these techniques can yield. Focusing on actin-based motility systems and drawing on the latest advances in the techniques of biochemistry, biophysics, microscopy, computer-assisted motion analysis, and molecular genetics, the works in this collection will prove invaluable to future research in embryogenesis, cancer, and diseases related to the cellular immune system.

Among the topics covered:

• Bacterial motility and chemotaxis
• New technologies for characterizing motility-related parameters
• Methods for analyzing the cortical tension of normal cells compared to abnormal cells
• Model for how a cell extends a pseudopod
• Computer-assisted analyses of cytoskeletal mutants of Dictyostelium discoideum
• Emerging methods for studying how cells respond to topographical cues at the substratum
• Polymerization of host actin to facilitate propulsion
• Genetic approaches to the regulatory mechanisms involved in early zebra fish morphogenesis and specification of cell fates
• Specific examples of single cell motility in embryogenesis
• Research oriented toward understanding cancer cell arrest, extravasation, and migration

A groundbreaking treatment of motion analysis of animal cells, Motion Analysis of Living Cells is a thorough review for professionals as well as a comprehensive introduction for students and researchers in the field. It is must reading for cell and developmental biologists, microbiologists, immunologists, neuroscientists, cancer researchers, zoologists, and basic researchers in reproductive medicine.

Die meisten aktiven Zellfunktionen beinhalten Bewegung. Von so subtilen subzellulären Vorgängen wie dem Sortieren und Ansteuern von Vesikeln und Organellen, die zur Freisetzung von Hormonen führen, bis hin zu den komplexen Neuordnungen einer Sphäre embryonaler Zellen, die den Körperplan eines vollständigen Organismus bilden, wird die Beweglichkeit von Zellen in einem breiten Spektrum beobachtbarer Dinge gezeigt Verhaltensweisen. Trotz dieses Phänomens basierten die Ansichten der modernen Biologie jedoch historisch gesehen auf statischen Bildern und Modellen, die auf biochemischen Beweisen beruhten.

Motion Analysis of Living Cells ist der erste Band, der die neuesten Forschungsergebnisse prominenter Spezialisten der Molekular- und Zellbiologie zusammenstellt und neu entwickelte Techniken zur Untersuchung zellulärer Bewegungen sowie kritische Analysen der Informationen vorstellt, die diese Techniken liefern können. Die Arbeiten in dieser Sammlung konzentrieren sich auf aktinbasierte Motilitätssysteme und stützen sich auf die neuesten Fortschritte in den Techniken der Biochemie, Biophysik, Mikroskopie, computergestützten Bewegungsanalyse und Molekulargenetik. Sie werden sich für die zukünftige Forschung in den Bereichen Embryogenese, Krebs usw. als unschätzbar wertvoll erweisen Erkrankungen im Zusammenhang mit dem zellulären Immunsystem.

Zu den behandelten Themen gehören:

• Bakterienmotilität und Chemotaxis
• Neue Technologien zur Charakterisierung motilitätsbezogener Parameter
• Methoden zur Analyse der kortikalen Spannung normaler Zellen im Vergleich zu abnormalen Zellen
• Modell dafür, wie eine Zelle einen Pseudopoden erweitert
• Computergestützte Analysen von Zytoskelettmutanten von Dictyostelium discoideum
• Neue Methoden zur Untersuchung, wie Zellen auf topografische Hinweise am Substrat reagieren
• Polymerisation von Wirtsaktin zur Erleichterung des Antriebs
• Genetische Ansätze zu den Regulierungsmechanismen, die an der frühen Morphogenese von Zebrafischen und der Spezifizierung des Zellschicksals beteiligt sind
• Spezifische Beispiele der Einzelzellmotilität in der Embryogenese
• Forschung, die auf das Verständnis des Stillstands, der Extravasation und der Migration von Krebszellen ausgerichtet ist

Motion Analysis of Living Cells ist eine bahnbrechende Behandlung der Bewegungsanalyse tierischer Zellen und eine gründliche Übersicht für Fachleute sowie eine umfassende Einführung für Studenten und Forscher auf diesem Gebiet. Es ist eine Pflichtlektüre für Zell- und Entwicklungsbiologen, Mikrobiologen, Immunologen, Neurowissenschaftler, Krebsforscher, Zoologen und Grundlagenforscher der Reproduktionsmedizin.

Bacterial Motility and Chemotaxis (J. Armitage & H. Packer).

Two- and Three-Dimensional Computer Systems for Analyzing How Animal Cells Crawl (D. Soll & E. Voss).

Toward the Automated Dissection of Morphological and Molecular Dynamics of Living Normal and Transformed Cells (K. Guiliano, et al.).

Forces and Mechanical Properties in Cell Locomotion (E. Elson, et al.).

The Biochemistry of Animal Cell Crawling (J. Condeelis).

Computer-Assisted Characterization of the Behavioral Defects of Cytoskeletal Mutants of Dictyostelium discoideum (D. Wessels & D. Soll).

Topographical Control of Cell Migration (A. Curtis & C. Wilkinson).

Movement of Bacterial Pathogens Driven by Actin Polymerization (D. Fung & J. Theriot).

Morphogenetic Cell Behaviors and Specification of Cell Fate During Early Teleost Development (M. Cooper & C. Kimmel).

Neural Crest Migration (C. Krull & M. Bronner-Fraser).

Neuronal Growth Cone Motility: Contributions from Neurogenetic Analyses of Cultured Drosophila Neurons (C. Wu).

In Vivo Videomicroscopy of Experimental Hematogenous Metastasis: Cancer Cell Arrest, Extravasation, and Migration (I. MacDonald, et al.).

Index.

David R. Soll is a Professor of Biology at the University of Iowa. He is best known for the motion analysis of living cells, the discovery of Candida albicans phenotypic switching and monoclonal antibody technology. Deborah Wessels is the editor of Motion Analysis of Living Cells, published by Wiley. Most active cellular functions involve motion. From such subtle subcellular actions as the sorting and targeting of vesicles and organelles leading to the release of hormones to the complex rearrangements of a sphere of embryonic cells forming the body plan of a complete organism, motility of cells is exhibited along a wide range of observable behaviors. Despite this phenomenon, however, the views of modern biology historically have been based on static images and models constructed from biochemical evidence.

Motion Analysis of Living Cells is the first volume to compile the latest research by prominent specialists in molecular and cell biology, presenting newly developed techniques for studying cellular motions and critical analyses of the information these techniques can yield. Focusing on actin-based motility systems and drawing on the latest advances in the techniques of biochemistry, biophysics, microscopy, computer-assisted motion analysis, and molecular genetics, the works in this collection will prove invaluable to future research in embryogenesis, cancer, and diseases related to the cellular immune system.

Among the topics covered:
* Bacterial motility and chemotaxis
* New technologies for characterizing motility-related parameters
* Methods for analyzing the cortical tension of normal cells compared to abnormal cells
* Model for how a cell extends a pseudopod
* Computer-assisted analyses of cytoskeletal mutants of Dictyostelium discoideum
* Emerging methods for studying how cells respond to topographical cues at the substratum
* Polymerization of host actin to facilitate propulsion
* Genetic approaches to the regulatory mechanisms involved in early zebra fish morphogenesis and specification of cell fates
* Specific examples of single cell motility in embryogenesis
* Research oriented toward understanding cancer cell arrest, extravasation, and migration

A groundbreaking treatment of motion analysis of animal cells, Motion Analysis of Living Cells is a thorough review for professionals as well as a comprehensive introduction for students and researchers in the field. It is must reading for cell and developmental biologists, microbiologists, immunologists, neuroscientists, cancer researchers, zoologists, and basic researchers in reproductive medicine.