{"product_id":"molecular-analysis-and-genome-discovery-isbn-9780470758779","title":"Molecular Analysis and Genome Discovery","description":"\u003ci\u003eMolecular Analysis and Genome Discovery\u003c\/i\u003e, Second Edition is a completely revised and updated new edition of this successful book. The text provides a comprehensive overview of recent developments in the fast moving field of molecular based diagnostics of disease markers. Key concepts and applications are provided alongside practical information on current techniques currently being researched and developed. Each chapter offers an up-to-date analysis of the subject encompassing the very latest technology platforms and is an essential reference for researchers in the field looking for an up-to-date overview of the subject. The book will also be an indispensable resource for those working in the biotechnology and pharmaceutical industries. New for this edition: chapters on Genotyping through Mutation Detection; Differential Gene Expression; Haplotyping and Molecular Profiling.  \u003cb\u003ePreface ix\u003c\/b\u003e  \u003cp\u003e\u003cb\u003eContributors xi\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Overview of Genotyping 1\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eMonica Bayes and Ivo Glynne Gut\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 1\u003c\/p\u003e \u003cp\u003eMethods for interrogating SNPs 3\u003c\/p\u003e \u003cp\u003eCommercial platforms for SNP genotyping 7\u003c\/p\u003e \u003cp\u003ePractical recommendations 11\u003c\/p\u003e \u003cp\u003eSNP databases 14\u003c\/p\u003e \u003cp\u003eMethylation analysis 15\u003c\/p\u003e \u003cp\u003eCopy number variation analysis 16\u003c\/p\u003e \u003cp\u003eSecond generation sequencing technologies 17\u003c\/p\u003e \u003cp\u003eConclusions 18\u003c\/p\u003e \u003cp\u003eReferences 19\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 DNA Chip Analysis in Genome Discovery 24\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eRoss N. Nazar and Jane Robb\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 24\u003c\/p\u003e \u003cp\u003eInterrogating a genome 25\u003c\/p\u003e \u003cp\u003eCross-species hybridization 28\u003c\/p\u003e \u003cp\u003eComparative genomic hybridization and microarray-based genotyping 31\u003c\/p\u003e \u003cp\u003eBarcodes, DNA microarrays and organism identification 34\u003c\/p\u003e \u003cp\u003eConcluding remarks 36\u003c\/p\u003e \u003cp\u003eReferences 37\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 qPCR, Theory, Reliability and Use in Molecular Analysis 43\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJamie Murphy and Stephen A. Bustin\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eSample preparation 44\u003c\/p\u003e \u003cp\u003eRNA quality 45\u003c\/p\u003e \u003cp\u003eReagents 46\u003c\/p\u003e \u003cp\u003eAssay design 47\u003c\/p\u003e \u003cp\u003eTransparency of published data 51\u003c\/p\u003e \u003cp\u003eFurther considerations 52\u003c\/p\u003e \u003cp\u003eConclusion 52\u003c\/p\u003e \u003cp\u003eReferences 52\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 DNA Analysis in Droplet-Based Microfluidic Devices 56\u003cbr\u003e \u003c\/b\u003e\u003ci\u003ePinar Ozdemir and Yonghao Zhang\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 56\u003c\/p\u003e \u003cp\u003eContinuous-flow microPCR chips 58\u003c\/p\u003e \u003cp\u003ePCR inhibition and carryover contamination 62\u003c\/p\u003e \u003cp\u003ePCR in droplets 65\u003c\/p\u003e \u003cp\u003eConclusions 73\u003c\/p\u003e \u003cp\u003eReferences 75\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 High-Resolution Melt Profiling 81\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eSteven F. Dobrowolski and Carl T. Wittwer\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 81\u003c\/p\u003e \u003cp\u003eBasic concepts of melt profiling 82\u003c\/p\u003e \u003cp\u003eHRMP and polymerase chain reaction 84\u003c\/p\u003e \u003cp\u003eDNA specimens and HRMP 89\u003c\/p\u003e \u003cp\u003eDetermining a temperature window for melting 90\u003c\/p\u003e \u003cp\u003eDyes and platforms for melt profiling 92\u003c\/p\u003e \u003cp\u003eScanning PCR products for sequence variation 93\u003c\/p\u003e \u003cp\u003eGenotyping with high-resolution melt profiling 102\u003c\/p\u003e \u003cp\u003eOther applications of HRMP 108\u003c\/p\u003e \u003cp\u003eFinal notes 109\u003c\/p\u003e \u003cp\u003eReferences 110\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Massively Parallel Sequencing 114\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eTracy Tucker, Marco Marra and Jan M. Friedman\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eSanger sequencing 114\u003c\/p\u003e \u003cp\u003eMassively parallel sequencing 114\u003c\/p\u003e \u003cp\u003eCommercially available massively parallel sequencers 115\u003c\/p\u003e \u003cp\u003eFuture technologies 122\u003c\/p\u003e \u003cp\u003ePaired-end or mate-paired reads 123\u003c\/p\u003e \u003cp\u003eTarget-enrichment strategies for MPS 124\u003c\/p\u003e \u003cp\u003eApplications of MPS 125\u003c\/p\u003e \u003cp\u003eSummary 131\u003c\/p\u003e \u003cp\u003eReferences 132\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Aptamers for Analysis: Nucleic Acids Ligands in the Post-Genomic Era 135\u003cbr\u003e \u003c\/b\u003e\u003ci\u003ePedro Nadal, Alessandro Pinto, Marketa Svobodova and Ciara K. O'Sullivan\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 135\u003c\/p\u003e \u003cp\u003eSELEX 136\u003c\/p\u003e \u003cp\u003eAptamers in analysis 147\u003c\/p\u003e \u003cp\u003eImaging with aptamers 157\u003c\/p\u003e \u003cp\u003eConclusions, outlooks and perspectives 160\u003c\/p\u003e \u003cp\u003eReferences 161\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Use of Nanotechnology for Enhancing of Cancer Biomarker Discovery and Analysis: A Molecular Approach 175\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eFarid E. Ahmed\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 175\u003c\/p\u003e \u003cp\u003eProteomics and nanotechnology 176\u003c\/p\u003e \u003cp\u003eNanoscale multicomponent separation 176\u003c\/p\u003e \u003cp\u003eNanoscale protein detection strategies 178\u003c\/p\u003e \u003cp\u003eSurface-enhanced Raman scattering (SERS) 182\u003c\/p\u003e \u003cp\u003eReferences 188\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Chip-Based Proteomics 193\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJulian Bailes, Andrew Milnthorpe, Sandra Smieszek and Mikhail Soloviev\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 193\u003c\/p\u003e \u003cp\u003eLab-on-a-chip 194\u003c\/p\u003e \u003cp\u003eArrays 196\u003c\/p\u003e \u003cp\u003eChip-based mass spectrometry 203\u003c\/p\u003e \u003cp\u003eSurface plasmon resonance (SPR) and quartz crystal microbalance (QCM) chip instruments 205\u003c\/p\u003e \u003cp\u003eMicrofluidics 207\u003c\/p\u003e \u003cp\u003eConclusion 209\u003c\/p\u003e \u003cp\u003eReferences 211\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Antibody Microarrays in Proteome Profiling 219\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eMohamed Saiel Saeed Alhamdani and Jorg D. Hoheisel\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 219\u003c\/p\u003e \u003cp\u003eTechnical aspects 220\u003c\/p\u003e \u003cp\u003eAntibody array applications 226\u003c\/p\u003e \u003cp\u003eSummary 229\u003c\/p\u003e \u003cp\u003eAcknowledgements 229\u003c\/p\u003e \u003cp\u003eReferences 229\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Biomarker Detection and Molecular Profiling by Multiplex Microbead Suspension Array Based Immunoproteomics 244\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eV. V. Krishhan, Imran H. Khan and Paul A. Luciw\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 244\u003c\/p\u003e \u003cp\u003ePrinciples of microbead-based multiplexing 245\u003c\/p\u003e \u003cp\u003eExperimental aspects of the multiplex microbead assay 247\u003c\/p\u003e \u003cp\u003eMultiplex microbead assay design and comparison with other methods 250\u003c\/p\u003e \u003cp\u003eApplications of the multiplex microbead assay system for biomedical research and clinical studies 252\u003c\/p\u003e \u003cp\u003eSelected investigational fields for multiplex analysis and examples of applications 255\u003c\/p\u003e \u003cp\u003eChallenges and current limitations 255\u003c\/p\u003e \u003cp\u003eSummary and future directions 259\u003c\/p\u003e \u003cp\u003eAcknowledgements 261\u003c\/p\u003e \u003cp\u003eReferences 261\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Mass Spectrometry in Metabolomics 271\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eWilliam J. Griffiths and Yuqin Wang\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 271\u003c\/p\u003e \u003cp\u003eSample collection and preparation 272\u003c\/p\u003e \u003cp\u003eData acquisition 274\u003c\/p\u003e \u003cp\u003eData analysis 279\u003c\/p\u003e \u003cp\u003eApplications 283\u003c\/p\u003e \u003cp\u003eConclusion 294\u003c\/p\u003e \u003cp\u003eAcknowledgement 294\u003c\/p\u003e \u003cp\u003eReferences 295\u003c\/p\u003e \u003cp\u003e\u003cb\u003eIndex 299\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e“It will be of immense value to students, biotechnologists, and scientists in the pharmaceutical industry. This update absolutely justifies replacement of the first edition.”  (\u003ci\u003eDoody’s\u003c\/i\u003e, 24 August 2012)\u003c\/p\u003e  \u003cp\u003e\u003cstrong\u003eRalph Rapley, Ph.D.\u003c\/strong\u003e Department of Biosciences, University of Hertfordshire, Hatfield, UKElectrical Engineers, Mechanical Engineers, Automation Engineers, Optical Industry, Students in Electrical Engineering, Students in Physics, Robotics Engineers, Automation Industry, Sensor Industry, Automobile Industry, Aircraft and Space Industry, Company Libraries, University Institutes and Libraries, Industry.\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47989646786789,"sku":"NP9780470758779","price":149.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9780470758779.jpg?v=1761784946","url":"https:\/\/k12savings.com\/es\/products\/molecular-analysis-and-genome-discovery-isbn-9780470758779","provider":"K12savings","version":"1.0","type":"link"}