Methods in Animal Proteomics

The study of proteomics provides researchers with a better understanding of disease and physiological processes in animals. Methods in Animal Proteomics will provide animal scientists and veterinarians currently researching these topics in domestic animals a firm foundation in the basics of proteomics methodology, while also reviewing important advances that will be of interest to established researchers in the field. Chapters will provide practical information on a range of topics including protein identification and separation, bioinformatics, and applications to disease and reproduction research. This text will be written by leading international proteomics experts and essential for researchers in the fields of animal biology and veterinary medicine.

Contributors vii

Acknowledgments xi

Section 1: Exploring Animal Proteomes 3

1 An Introduction to Animal Proteomics 5
Phillip D. Whitfield and P. David Eckersall

2 Types of Sample and Experimental Planning 11
Christine Olver

3 Protein Separation Strategies 41
Ingrid Miller

4 Methods and Approaches to Mass Spectroscopy-Based Protein Identification 77
John D. Lippolis and Timothy A. Reinhardt

5 Bioinformatics in Animal Proteomics 103
Paul Blakeley, James C. Wright, Simon J. Hubbard, and Andrew R. Jones

6 Comparative Proteomic Approaches 121
Johanna S. Rees and Kathryn S. Lilley

7 Advancing Technologies for Spatial and Temporal Proteomics 159
Mary Doherty

Section 2: Applications of Proteomics in Animal Biology 189

8 Proteomic Strategies to Investigate Adaptive Processes 191
L. Elaine Epperson and Sandra L. Martin

9 Investigation of Animal Venoms and Toxins 211
Stephen McClean

10 Proteomics in Animal Health and Disease 243
P. David Eckersall and Mark McLaughlin

11 Application of Proteomics to Elucidate Bacterium–Host Interactions 319
David G.E. Smith

12 Animal Parasitology and Proteomics 349
Richard Burchmore

13 Proteomics in Animal Reproduction and Breeding 369
Divyaswetha Peddinti, Erdogan Memili, and Shane C. Burgess

14 Assessment in the Quality and Safety of Food of Animal Origin 397
Begonya Marcos, Jingshun Liu, Dilip K. Rai, Alessio Di Luca, and Anne Maria Mullen

Index 431

Philip D. Whitfield is Head of Lipodomic Research at the University of the Highlands and Islands Department of Diabetes and Cardiovascular Science

David Eckersall is Professor of Veterinary Biochemistry University of Glasgow.

The study of proteomics provides researchers with a better understanding of disease and physiological processes in animals. Methods in Animal Proteomics will provide animal scientists and veterinarians currently researching these topics in domestic animals a firm foundation in the basics of proteomics methodology, while also reviewing important advances that will be of interest to established researchers in the field. Chapters will provide practical information on a range of topics including protein identification and separation, bioinformatics, and applications to disease and reproduction research. This text will be written by leading international proteomics experts and essential for researchers in the fields of animal biology and veterinary medicine.