{"product_id":"manual-of-research-techniques-in-cardiovascular-medicine-isbn-9780470672693","title":"Manual of Research Techniques in Cardiovascular Medicine","description":"While some research methods or techniques are applicable in several areas of medicine, research in cardiovascular diseases requires knowledge of an increasing array of procedures, techniques and measurements that are highly specialized and unique to this area of investigation. Edited by senior clinical investigators who are recognized leaders in cardiovascular medicine worldwide, this book provides readers with a comprehensive, practical “how-to-do-it” review of best-practice techniques for cardiovascular research.  \u003cp\u003e\u003ci\u003eList of Contributors ix\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003ePreface xix\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eAbout the Companion Website xx\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart 1 Electrophysiology\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1 Measurement of calcium transient ex vivo 3\u003cbr\u003e Kenneth R. Laurita and Bradley N. Plummer\u003c\/p\u003e \u003cp\u003e2 Confocal imaging of intracellular calcium cycling in isolated cardiac myocytes 12\u003cbr\u003e Søren Grubb, J. Andrew Wasserstrom, and Gary L. Aistrup\u003c\/p\u003e \u003cp\u003e3 Generating a large animal model of persistent atrial fi brillation 20\u003cbr\u003e Raphaël P. Martins and José Jalife\u003c\/p\u003e \u003cp\u003e4 Confocal imaging of intracellular calcium cycling in the intact heart 32\u003cbr\u003e Neha Singh, Manvinder Kumar, James E. Kelly, Gary L. Aistrup, and J. Andrew Wasserstrom\u003c\/p\u003e \u003cp\u003e5 Recording and measurement of action potentials 41\u003cbr\u003e Kenneth S. Ginsburg and Donald M. Bers\u003c\/p\u003e \u003cp\u003e6 Patch-clamp recordings from isolated cardiac myocytes 50\u003cbr\u003e Kai-Chien Yang, Wei Wang, and Jeanne M. Nerbonne\u003c\/p\u003e \u003cp\u003e7 Optical mapping of the heart 60\u003cbr\u003e Di Lang, Sarah Gutbrod, Jacob Laughner, and Igor R. Efi mov\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart 2 Isolation and Maintenance of Primary Stem Cells\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8 Isolation of colony-forming endothelial progenitor cells 71\u003cbr\u003e Haruchika Masuda and Takayuki Asahara\u003c\/p\u003e \u003cp\u003e9 Cardiac resident stem cells 87\u003cbr\u003e João Ferreira-Martins, Fumihiro Sanada, and Marcello Rota\u003c\/p\u003e \u003cp\u003e10 Cardiospheres 95\u003cbr\u003e Rachel Ruckdeschel Smith\u003c\/p\u003e \u003cp\u003e11 Mesenchymal stem cells 104\u003cbr\u003e Jose S. Da Silva and Joshua M. Hare\u003c\/p\u003e \u003cp\u003e12 Generation and differentiation of human iPS cells 110\u003cbr\u003e Sebastian Diecke, Lei Ye, Sophia Zhang, and Jianyi Zhang\u003c\/p\u003e \u003cp\u003e13 Isolation of neonatal and adult rat cardiomyocytes 117\u003cbr\u003e Md. Abdur Razzaque and Jeffrey Robbins\u003c\/p\u003e \u003cp\u003e14 Isolation and culture of vascular smooth muscle cells 125\u003cbr\u003e Milton Hamblin, Lin Chang, and Y. Eugene Chen\u003c\/p\u003e \u003cp\u003e15 Isolation and culture of cardiac endothelial cells 131\u003cbr\u003e Asish K. Ghosh, Joseph W. Covington, and Douglas E. Vaughan\u003c\/p\u003e \u003cp\u003e16 Isolation and culture of cardiac fi broblasts 140\u003cbr\u003e Asish K. Ghosh, Joseph W. Covington and Douglas E. Vaughan\u003c\/p\u003e \u003cp\u003e17 Murine bone marrow transplantation model 146\u003cbr\u003e Prasanna Krishnamurthy, Suresh Kumar Verma, and Raj Kishore\u003c\/p\u003e \u003cp\u003e18 In vitro differentiation and expansion of vascular endothelial cells derived from mouse embryonic stem cells 149\u003cbr\u003e Anees Fatima, Carey Nassano-Miller, and Tsutomu Kume\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart 3 Manipulation of the Heart and Vessels in Vivo and ex Vivo\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e19 Coronary ligation 157\u003cbr\u003e Alexander R. Mackie and Hossein Ardehali\u003c\/p\u003e \u003cp\u003e20 Transverse aortic constriction: a model to study heart failure in small animals 164\u003cbr\u003e Suresh Kumar Verma, Prasanna Krishnamurthy, and Raj Kishore\u003c\/p\u003e \u003cp\u003e21 Pharmacological models of hypertrophy and failure 170\u003cbr\u003e Angela C. deAlmeida, Tariq Hamid, and Sumanth D. Prabhu\u003c\/p\u003e \u003cp\u003e22 Hindlimb ischemia 177\u003cbr\u003e Jerry C. Lee, Ngan F. Huang, and John P. Cooke\u003c\/p\u003e \u003cp\u003e23 The Langendorff preparation 187\u003cbr\u003e Hugh Clements-Jewery and Michael J. Curtis\u003c\/p\u003e \u003cp\u003e24 Myocarditis and other immunological models of cardiac disease 197\u003cbr\u003e Daniela C¡ iháková and Noel R. Rose\u003c\/p\u003e \u003cp\u003e25 Models of pacing-induced heart failure 203\u003cbr\u003e James A. Shuman, Rupak Mukherjee, and Francis G. Spinale\u003c\/p\u003e \u003cp\u003e26 Porcine myocardial ischemia models 215\u003cbr\u003e Xian-Liang Tang and Roberto Bolli\u003c\/p\u003e \u003cp\u003e27 Angiogenesis assays 225\u003cbr\u003e Susmita Sahoo and Douglas W. Losordo\u003c\/p\u003e \u003cp\u003e28 Immunohistochemical analysis of cardiac tissue 232\u003cbr\u003e Barbara Ogórek, Donato Cappetta, and Jan Kajstura\u003c\/p\u003e \u003cp\u003e29 A murine model of cardiac arrest by exsanguination 237\u003cbr\u003e Guangming Cheng, Yiru Guo, Harold K. Elias, Carrie M. Quinn, Arash Davani, Yanjuan Yang, Magdy Girgis, Roberto Bolli, and Buddhadeb Dawn\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart 4 Small Animal Imaging\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e30 Blood pressure, telemetry, and vascular measurements in the rodent model 245\u003cbr\u003e Robert S. Danziger\u003c\/p\u003e \u003cp\u003e31 The setting: imaging conscious, sedated, or anesthetized rodents 250\u003cbr\u003e Gene H. Kim and Roberto M. Lang\u003c\/p\u003e \u003cp\u003e32 Echocardiography: standard techniques (M-mode, two-dimensional imaging, and Doppler) 254\u003cbr\u003e Gene H. Kim, Lauren Beussink-Nelson, Sanjiv J. Shah, and Roberto M. Lang\u003c\/p\u003e \u003cp\u003e33 Echocardiography: advanced techniques (tissue Doppler, speckle tracking, and three-dimensional imaging) 275\u003cbr\u003e Lauren Beussink-Nelson, Gene H. Kim, Roberto M. Lang, and Sanjiv J. Shah\u003c\/p\u003e \u003cp\u003e34 In vivo tomographic cardiac imaging: positron emission tomography and magnetic resonance imaging 287\u003cbr\u003e Bruno C. Huber, Patricia K. Nguyen, and Joseph C. Wu\u003c\/p\u003e \u003cp\u003e35 In vivo hemodynamics 295\u003cbr\u003e Alexander R. Mackie, Kyle K. Henderson, Sol Misener, and Hossein Ardehali\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart 5 Metabolism, Mitochondria, and Cell Death\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e36 Fractionation of cardiomyocytes and isolation of mitochondria 307\u003cbr\u003e Christopher P. Baines\u003c\/p\u003e \u003cp\u003e37 Assessment of glucose and fatty acid metabolism ex vivo 313\u003cbr\u003e Darrell D. Belke and E. Dale Abel\u003c\/p\u003e \u003cp\u003e38 Quantification and characterization of atherosclerotic lesions in mice 320\u003cbr\u003e Abhinav Agarwal, Millicent G. Winner, Srinivas D. Sithu, and Sanjay Srivastava\u003c\/p\u003e \u003cp\u003e39 Assessment of cell death in the heart 332\u003cbr\u003e Russell S. Whelan, Klitos Konstantinidis, and Richard N. Kitsis\u003c\/p\u003e \u003cp\u003e40 Assessment of mitochondrial function in isolated cells 343\u003cbr\u003e Amy K. Rines and Hossein Ardehali\u003c\/p\u003e \u003cp\u003e41 Multinuclear NMR spectroscopy of myocardial energetics and substrate utilization in isolated perfused mouse hearts 351\u003cbr\u003e Stephen C. Kolwicz, Jr. and Rong Tian\u003c\/p\u003e \u003cp\u003e42 Measurement of reactive oxygen species in cardiovascular disease 359\u003cbr\u003e Mahmood Khan, Fatemat Hassan, Sashwati Roy, and Chandan K. Sen\u003c\/p\u003e \u003cp\u003e43 Assessing autophagy 371\u003cbr\u003e Roberta A. Gottlieb\u003c\/p\u003e \u003cp\u003e44 Assessment of cardiomyocyte size 378\u003cbr\u003e A. Martin Gerdes and Alessandro Pingitore\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart 6 Manipulation of Gene Expression in Vitro and in Vivo\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e45 Generation of Cre-loxP mouse models for conditional knockout and overexpression of genes in various heart cells 389\u003cbr\u003e Marisa Z. Jackson and Warren G. Tourtellotte\u003c\/p\u003e \u003cp\u003e46 Modulation of myocardial genes via use of adenoviral vectors and RNA interference approaches 397\u003cbr\u003e Qianhong Li\u003c\/p\u003e \u003cp\u003e47 Overexpression and downregulation of proteins in vitro 407\u003cbr\u003e Marina Bayeva and Hossein Ardehali\u003c\/p\u003e \u003cp\u003e48 In vivo microRNA studies 416\u003cbr\u003e Eva van Rooij\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart 7 Model Systems\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e49 Vascular and cardiac studies in zebrafi sh 425\u003cbr\u003e Hans-Georg Simon, Molly Ahrens, and Brandon Holtrup\u003c\/p\u003e \u003cp\u003e50 Vascular and cardiac studies in Drosophila 432\u003cbr\u003e Lin Yu, Joseph P. Daniels, and Matthew J. Wolf\u003c\/p\u003e \u003cp\u003e\u003ci\u003eIndex 441\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003eEdited by\u003cbr\u003e \u003cbr\u003e \u003cb\u003eHossein Ardehali MD, PhD, FAHA\u003c\/b\u003e, Associate Professor of Medicine, Molecular Pharmacology and Biological\u003cbr\u003e Chemistry\u003cbr\u003e Feinberg Cardiovascular Research Institute\u003cbr\u003e Northwestern University\u003cbr\u003e Feinberg School of\u003cbr\u003e Medicine, Chicago, IL USA\u003c\/p\u003e \u003cp\u003e\u003cb\u003eRoberto Bolli MD\u003c\/b\u003e\u003cbr\u003e Professor of Medicine Chief, Division of Cardiovascular Medicine\u003cbr\u003e Director, Institute of Molecular Cardiology\u003cbr\u003e University of Louisville\u003cbr\u003e Louisville, KY USA\u003c\/p\u003e \u003cp\u003e\u003cb\u003eDouglas W. Losordo MD\u003c\/b\u003e\u003cbr\u003e Vice President New Therapeutic Development\u003cbr\u003e Baxter Healthcare Corporation\u003cbr\u003e Deerfield, IL USA\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e  \u003cp\u003eWhile some aspects of clinical and scientific research are common across medicine, research in cardiovascular diseases requires knowledge of an increasing array of procedures, techniques, models and measurements that are highly specialized and unique to this area of investigation. Edited and written by senior investigators, many of whom have been involved in pioneering studies in cardiovascular disease, this new book provides today’s cardiology research professionals with practical how-to guidance on designing, conducting, measuring and reporting their research.\u003c\/p\u003e \u003cp\u003e\u003ci\u003eManual of Research Techniques in Cardiovascular Medicine\u003c\/i\u003e:\u003c\/p\u003e \u003cp\u003e• Reviews the must-know techniques, methods, analyses, models and measurements of cardiovascular research in a practical, “how-to-do-it” approach, resulting in clear-headed guidance to even the most complicated techniques and methods.\u003c\/p\u003e \u003cp\u003e• Covers each technique or method beginning with an explanation of when and why it should be selected\u003cbr\u003e followed by step-by-step instructions that include why each step is important; what can go wrong; how to trouble shoot and correct errors, and how to analyze and report results.\u003c\/p\u003e \u003cp\u003e• Helps ensure research success, but also, critically, enables readers to understand why and how some experiments fail.\u003c\/p\u003e \u003cp\u003e• Contains illustrative figures to reinforce the key components\/steps of each method covered.\u003c\/p\u003e \u003cp\u003e• Includes a companion website with video clips to support the discussion of particularly challenging techniques, for example animal surgical procedures.\u003cbr\u003e \u003cbr\u003e \u003ci\u003eManual of Research Techniques in Cardiovascular Medicine\u003c\/i\u003e is an invaluable resource for investigators at all levels, from technicians, students, fellows and other healthcare professionals who are approaching research for the first time, to seasoned investigators in cardiovascular disease, pharmacology, and related areas who need expert guidance on emerging techniques or unfamiliar models or methods.\u003c\/p\u003e","brand":"Wiley-Blackwell","offers":[{"title":"Default Title","offer_id":47989571223781,"sku":"NP9780470672693","price":197.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9780470672693.jpg?v=1761784643","url":"https:\/\/k12savings.com\/es\/products\/manual-of-research-techniques-in-cardiovascular-medicine-isbn-9780470672693","provider":"K12savings","version":"1.0","type":"link"}