{"product_id":"manual-of-molecular-microbiology-isbn-9781683674566","title":"Manual of Molecular Microbiology","description":"\u003cp\u003e\u003cb\u003eYour essential guide to the design, operation, management, and health care integration of the modern molecular microbiology laboratory.\u003c\/b\u003e \u003c\/p\u003e\u003cp\u003eThis comprehensive resource offers definitive guidance on the operational and interpretive aspects of clinical molecular microbiology. Tailored for medical laboratory professionals, it provides practical “how-to” guidance for establishing, maintaining, and advancing molecular microbiology testing services and details the unique expertise required to support infectious disease diagnostics. \u003c\/p\u003e\u003cp\u003eThe \u003ci\u003eManual\u003c\/i\u003e offers a clear and practical roadmap for topics ranging from selecting appropriate technologies, instruments, and analytic pipelines to navigating complex interpretive challenges and positioning diagnostic testing services for future clinical and population health needs. \u003c\/p\u003e\u003cp\u003eBeginning with foundational technologies and their clinical applications, this book offers accessible overviews of each method's potential, implications, and emerging roles. Subsequent sections dive meticulously into details of laboratory setup, design, and operations, empowering readers with hands-on insights for routine and advanced testing methods, including advanced sequencing technologies. It also tackles the nuanced challenges of interpreting and reporting results from cutting-edge diagnostics, including those focused on antimicrobial resistance and metagenomics. \u003c\/p\u003e\u003cp\u003eThe final section explores the broader impact of molecular microbiology on value-based care, with discussions on clinical management, laboratory stewardship, and the future of molecular diagnostics in public health. \u003c\/p\u003e\u003cp\u003eComprehensive and forward-looking, the \u003ci\u003eManual of Molecular Microbiology\u003c\/i\u003e equips readers with both foundational knowledge and practical expertise, making it an indispensable reference for today's clinical laboratory professionals. \u003c\/p\u003e\u003cp\u003eContributors vii\u003c\/p\u003e \u003cp\u003ePreface xi\u003c\/p\u003e \u003cp\u003eAuthor and Editor Conflicts of Interest xiii\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSection I Technologies and Clinical Applications 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1 Real-Time PCR 3\u003cbr\u003e \u003ci\u003eJocelyn Hauser, Eleanor Powell, And N. Esther Babady\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2 Digital PCR 18\u003cbr\u003e \u003ci\u003eAlexandra S. Whale, Denise M. O’sullivan, And Jim F. Huggett\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3 Isothermal Nucleic Acid Amplification Technologies and CRISPR-Cas-Based Nucleic Acid Detection\u003c\/p\u003e \u003cp\u003eStrategies for Infectious Diseases Diagnostics 30\u003cbr\u003e \u003ci\u003eBenjamin M. Liu\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e4 Strain Typing (Bacterial, Viral, Fungal, and Mycobacterial) 48\u003cbr\u003e \u003ci\u003eHelena M.b. Seth-smith, Ashley M. Rooney, And Adrian Egli\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5 Antimicrobial Resistance Genotyping 74\u003cbr\u003e \u003ci\u003eHeba H. Mostafa and Patricia J. Simner\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6 Broad-Range Diagnostic Amplification and Detection 94\u003cbr\u003e \u003ci\u003eØyvind Kommedal and Ruben Dyrhovden\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7 Characterization of the Human Microbiome in Health and Disease 110\u003cbr\u003e \u003ci\u003eChin Yee Tan and Neeraj K. Surana\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e8 Host Transcriptomics: Clinical Applications 127\u003cbr\u003e \u003ci\u003eAsuncion Mejias and Octavio Ramilo\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e9 Organism Transcriptomics 137\u003cbr\u003e \u003ci\u003eAryeh Solomon and Roi Avraham\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSection II Design, Implementation, and Operations: General 145\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e10 Laboratory Design for Molecular Technologies 147\u003cbr\u003e \u003ci\u003eErin Graf\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e11 Sample Selection, Preservation, Transport, and Storage 153\u003cbr\u003e \u003ci\u003eJames J. Dunn and Erin H. Graf\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e12 Nucleic Acid Extraction and Purity, Including Performance Considerations of Different Extraction\u003c\/p\u003e \u003cp\u003eChemistries 164\u003cbr\u003e \u003ci\u003eBijal A. Parikh and Neil W. Anderson\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e13 Selection of Nucleic AcidAmplification Systems: In Vitro Diagnostic Tests 174\u003cbr\u003e \u003ci\u003eCecilia M. Thompson, Heba H. Mostafa, And Kyle G. Rodino\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e14 Selection of Nucleic Acid Amplification Systems: Laboratory Developed Tests 180\u003cbr\u003e \u003ci\u003eMeghan W. Starolis\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e15 Verification, Validation, and Quality Assurance for Nucleic Acid Amplification Tests 189\u003cbr\u003e \u003ci\u003ePaige M.k. Larkin, Ninad Mehta, And Rebekah M. Martin\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e16 Molecular Microbiology Standardization 203\u003cbr\u003e \u003ci\u003eJerry Boonyaratanakornkit\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSection III Design, Implementation, and Operations: Sequencing-Based Technologies 215\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e17 Sequencing Platforms 217\u003cbr\u003e \u003ci\u003eHege Vangstein Aamot, Jacob Moran-Gilad, and John W. A. Rossen\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e18 Nucleic Acid Extraction and Next-Generation Sequencing Library Prep 232\u003cbr\u003e \u003ci\u003eAbraham G. Moller, David C. Gaston, And Augusto Dulanto Chiang\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e19 Considerations of Depth, Coverage, and Other Read Quality Metrics 244\u003cbr\u003e \u003ci\u003eDerek D. N. Smith and Andrea D. Tyler\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e20 Pipelines and Databases—Genome Assembly and Analysis from Bacterial Isolates 262\u003cbr\u003e \u003ci\u003eNatalie Scherff, Hauke Tönnies, Gabriel Elias Wagner, and Alexander Mellmann\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e21 Analysis of Metagenomic Next-Generation Sequencing Data Obtained from Clinical Samples 278\u003cbr\u003e \u003ci\u003eBrian O’donovan, Steve Miller, And Katrina Kalantar\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e22 Pipelines and Databases— Microbiome Analysis 296\u003cbr\u003e \u003ci\u003eJonathan Sanders, Huang Lin, Qiyun Zhu, And James T. Morton\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e23 Validation and Quality Control of Next-Generation Sequencing Assays 314\u003cbr\u003e \u003ci\u003eRobert Schlaberg\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e24 Application-Specific Considerations for Clinical and Epidemiologic Sequencing 335\u003cbr\u003e \u003ci\u003eLydia A. Krasilnikova, Mohamad R. A. Sater, and Melis N. Anahtar\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e25 Generation and Analysis of Host Transcriptomics and Development of Host-Response Based Signatures  352\u003cbr\u003e \u003ci\u003eEmily C. Lydon, Katrina L. Kalantar, And Charles R. Langelier\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSection IV Special Challenges in Result Interpretation and Reporting 361\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e26 Bacterial Antimicrobial Susceptibility Testing 363\u003cbr\u003e \u003ci\u003eRomney Humphries\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e27 Public Health Surveillance 378\u003cbr\u003e \u003ci\u003eAllen Bateman and Sara J. Blosser\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e28 Considerations in Molecular Strain Typing for Bacteria, Fungi, and Viruses 391\u003cbr\u003e \u003ci\u003eS. Wesley Long\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e29 Considerations in Result Interpretation and Reporting for Untargeted and Broadly Targeted Pathogen\u003c\/p\u003e \u003cp\u003eDetection Sequencing Assays 398\u003cbr\u003e \u003ci\u003eHeather L. Glasgow and Steve Miller\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e30 Considerations in Result Interpretation and Reporting for Microbiome Analysis 407\u003cbr\u003e Arryn Craney\u003c\/p\u003e \u003cp\u003e31 Clinical Interpretation of Ultrasensitive Molecular Assays 422\u003cbr\u003e Daniel A. Ortiz and Blake W. Buchan\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSection V Molecular Microbiology’s Impact on Value-based Care 439\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e32 Clinical Interpretation and Diagnostic Management 441\u003cbr\u003e Swathi Gowtham\u003c\/p\u003e \u003cp\u003e33 The Role of Clinical Microbiology in Antimicrobial and Diagnostic Stewardship 456\u003cbr\u003e Amy J. Mathers\u003c\/p\u003e \u003cp\u003e34 Evidence-Based Assay Deployment— Support of Population Health Initiatives 467\u003cbr\u003e Susan M. Benson\u003c\/p\u003e \u003cp\u003e35 Evidence-Based Assay Deployment— Precision Medicine Initiatives 475\u003cbr\u003e Julie Hirschhorn and Haluk Kavus\u003c\/p\u003e \u003cp\u003e36 Evidence-Based Assay Deployment— Infrastructure, Documentation, and Strategy 483\u003cbr\u003e Natalie N. Whitfield and Donna M. Wolk\u003c\/p\u003e \u003cp\u003eIndex 499\u003c\/p\u003e  \u003cp\u003e\u003cb\u003eRandall T. Hayden,\u003c\/b\u003e St. Jude Children’s Research Hospital, Memphis, TN, USA. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eKaren C. Carroll,\u003c\/b\u003e The Johns Hopkins University School of Medicine, Baltimore, MD, USA. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eJohn P. Dekker,\u003c\/b\u003e National Institutes of Health, Bethesda, MD, USA. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eAlexander J. McAdam,\u003c\/b\u003e Boston Children’s Hospital, Boston, MA, USA. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eDonna M. Wolk,\u003c\/b\u003e Geisinger, Diagnostic Medicine Institute, Danville, PA; The Geisinger Commonwealth School of Medicine, Scranton, PA, USA.   \u003c\/p\u003e\u003cp\u003e\u003cb\u003eYour essential guide to design, operation, management, and health care integration of the modern molecular microbiology laboratory\u003c\/b\u003e \u003c\/p\u003e\u003cp\u003eThis comprehensive resource offers definitive guidance on the operational and interpretive aspects of clinical molecular microbiology. Tailored for medical laboratory professionals, it provides practical “how-to” guidance for establishing, maintaining, and advancing molecular microbiology testing services and details the unique expertise required to support infectious disease diagnostics. \u003c\/p\u003e\u003cp\u003eThe \u003ci\u003eManual\u003c\/i\u003e offers a clear and practical roadmap for topics ranging from selecting appropriate technologies, instruments, and analytic pipelines to navigating complex interpretive challenges and positioning diagnostic testing services for future clinical and population health needs. \u003c\/p\u003e\u003cp\u003eBeginning with foundational technologies and their clinical applications, this book offers accessible overviews of each method’s potential, implications, and emerging roles. Subsequent sections dive meticulously into details of laboratory setup, design, and operations, empowering readers with hands-on insights for routine and advanced testing methods, including advanced sequencing technologies. It also tackles the nuanced challenges of interpreting and reporting results from cutting-edge diagnostics, including those focused on antimicrobial resistance and metagenomics. \u003c\/p\u003e\u003cp\u003eThe final section explores the broader impact of molecular microbiology on value-based care, with discussions on clinical management, laboratory stewardship, and the future of molecular diagnostics in public health. \u003c\/p\u003e\u003cp\u003eComprehensive and forward-looking, the \u003ci\u003eManual of Molecular Microbiology \u003c\/i\u003eequips readers with both foundational knowledge and practical expertise, making it an indispensable reference for today’s clinical laboratory professionals.\u003c\/p\u003e","brand":"ASM Press","offers":[{"title":"Default Title","offer_id":47989570896101,"sku":"NP9781683674566","price":225.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9781683674566.jpg?v=1761784641","url":"https:\/\/k12savings.com\/es\/products\/manual-of-molecular-microbiology-isbn-9781683674566","provider":"K12savings","version":"1.0","type":"link"}