{"product_id":"magnetic-resonance-imaging-in-tissue-engineering-isbn-9781119193357","title":"Magnetic Resonance Imaging in Tissue Engineering","description":"\u003ci\u003eMagnetic Resonance Imaging in Tissue Engineering\u003c\/i\u003e provides a unique overview of the field of non-invasive MRI assessment of tissue engineering and regenerative medicine\u003cbr\u003e \u003cul\u003e \u003cli\u003eEstablish a dialogue between the tissue-engineering scientists and imaging experts and serves as a guide for tissue engineers and biomaterial developers alike\u003c\/li\u003e \u003cli\u003eProvides comprehensive details of magnetic resonance imaging (MRI) techniques used to assess a variety of engineered and regenerating tissues and organs\u003c\/li\u003e \u003cli\u003eCovers cell-based therapies, engineered cartilage, bone, meniscus, tendon, ligaments, cardiovascular, liver and bladder tissue engineering and regeneration assessed by MRI\u003c\/li\u003e \u003cli\u003eIncludes a chapter on oxygen imaging method that predominantly is used for assessing hypoxia in solid tumors for improving radiation therapy but has the ability to provide information on design strategies and cellular viability in tissue engineering regenerative medicine\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eList of Plates xiii\u003c\/p\u003e \u003cp\u003eAbout the Editors xix\u003c\/p\u003e \u003cp\u003eList of Contributors xxi\u003c\/p\u003e \u003cp\u003eForeword xxv\u003c\/p\u003e \u003cp\u003ePreface xxvii\u003c\/p\u003e \u003cp\u003eBook Summary xxxi\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart I Enabling Magnetic Resonance Techniques for Tissue Engineering Applications 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Stem Cell Tissue Engineering and Regenerative Medicine: Role of Imaging 3\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eBo Chen, Caleb Liebman, Parisa Rabbani, and Michael Cho\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e1.1 Introduction 3\u003c\/p\u003e \u003cp\u003e1.2 3D Biomimetics 5\u003c\/p\u003e \u003cp\u003e1.3 Assessment of Stem Cell Differentiation and Tissue Development 8\u003c\/p\u003e \u003cp\u003e1.4 Description of Imaging Modalities for Tissue Engineering 8\u003c\/p\u003e \u003cp\u003e1.4.1 Optical Microscopy 9\u003c\/p\u003e \u003cp\u003e1.4.2 Fluorescence Microscopy 9\u003c\/p\u003e \u003cp\u003e1.4.3 Multiphoton Microscopy 11\u003c\/p\u003e \u003cp\u003e1.4.4 Magnetic Resonance Imaging 14\u003c\/p\u003e \u003cp\u003eAcknowledgments 15\u003c\/p\u003e \u003cp\u003eReferences 15\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Principles and Applications of Quantitative Parametric MRI in Tissue Engineering 21\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eMrignayani Kotecha\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2.1 Introduction 21\u003c\/p\u003e \u003cp\u003e2.2 Basics of MRI 25\u003c\/p\u003e \u003cp\u003e2.2.1 Nuclear Spins 25\u003c\/p\u003e \u003cp\u003e2.2.2 Radio Frequency Pulse Excitation and Relaxation 28\u003c\/p\u003e \u003cp\u003e2.2.3 From MRS to MRI 31\u003c\/p\u003e \u003cp\u003e2.3 MRI Contrasts for Tissue Engineering Applications 32\u003c\/p\u003e \u003cp\u003e2.3.1 Chemical Shift 33\u003c\/p\u003e \u003cp\u003e2.3.2 Relaxation Times—T1 and T2 33\u003c\/p\u003e \u003cp\u003e2.3.3 Water Apparent Diffusion Coefficient 36\u003c\/p\u003e \u003cp\u003e2.3.4 Fractional Anisotropy 37\u003c\/p\u003e \u003cp\u003e2.4 X‐Nuclei MRI for Tissue Engineering Applications 38\u003c\/p\u003e \u003cp\u003e2.5 Preparing Engineered Tissues for MRI Assessment 38\u003c\/p\u003e \u003cp\u003e2.5.1 In Vitro Assessment 38\u003c\/p\u003e \u003cp\u003e2.5.2 In Vivo Assessment 39\u003c\/p\u003e \u003cp\u003e2.6 Limitations of MRI Assessment in Tissue Engineering 39\u003c\/p\u003e \u003cp\u003e2.7 Future Directions 40\u003c\/p\u003e \u003cp\u003e2.7.1 Biomolecular Nuclear Magnetic Resonance 40\u003c\/p\u003e \u003cp\u003e2.7.2 Cell–ECM–Biomaterial Interaction 40\u003c\/p\u003e \u003cp\u003e2.7.3 Quantitative MRI 40\u003c\/p\u003e \u003cp\u003e2.7.4 Standardization of MRI Methods for In Vitro and In Vivo Assessment 40\u003c\/p\u003e \u003cp\u003e2.7.5 Super‐Resolution MRI Techniques 41\u003c\/p\u003e \u003cp\u003e2.7.6 Magnetic Resonance Elastography 41\u003c\/p\u003e \u003cp\u003e2.7.7 Benchtop MRI 41\u003c\/p\u003e \u003cp\u003e2.8 Conclusions 41\u003c\/p\u003e \u003cp\u003eReferences 42\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 High Field Sodium MRS\/MRI: Application to Cartilage Tissue Engineering 49\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eMrignayani Kotecha\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introduction 49\u003c\/p\u003e \u003cp\u003e3.2 Sodium as an MR Probe 50\u003c\/p\u003e \u003cp\u003e3.3 Pulse Sequences 53\u003c\/p\u003e \u003cp\u003e3.3.1 Pulse Sequences for Measuring TSC 53\u003c\/p\u003e \u003cp\u003e3.3.2 TQC Pulse Sequences for Measuring ωQ and ω0τc 54\u003c\/p\u003e \u003cp\u003e3.4 Assessment of Tissue‐Engineered Cartilage 55\u003c\/p\u003e \u003cp\u003e3.4.1 Proteoglycan Assessment 57\u003c\/p\u003e \u003cp\u003e3.4.2 Assessment of Tissue Anisotropy and Molecular Dynamics 60\u003c\/p\u003e \u003cp\u003e3.4.3 Assessment of Osteochondral Tissue Engineering 61\u003c\/p\u003e \u003cp\u003e3.5 Sodium Biomarkers for Engineered Tissue Assessment 63\u003c\/p\u003e \u003cp\u003e3.5.1 Engineered Tissue Sodium Concentration (ETSC) 63\u003c\/p\u003e \u003cp\u003e3.5.2 Average Quadrupolar Coupling (ωQ) 64\u003c\/p\u003e \u003cp\u003e3.5.3 Motional Averaging Parameter (ω0τc) 64\u003c\/p\u003e \u003cp\u003e3.6 Future Directions 64\u003c\/p\u003e \u003cp\u003e3.7 Summary 64\u003c\/p\u003e \u003cp\u003eReferences 65\u003c\/p\u003e \u003cp\u003e4 SPIO‐Labeled Cellular MRI in Tissue Engineering: A Case Study in Growing Valvular Tissues 71\u003cbr\u003e \u003ci\u003eElnaz Pour Issa and Sharan Ramaswamy\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e4.1 Setting the Stage: A Clinical Problem Requiring a Tissue Engineering Solution 71\u003c\/p\u003e \u003cp\u003e4.2 SPIO Labeling of Cells 72\u003c\/p\u003e \u003cp\u003e4.2.1 Ferumoxides 72\u003c\/p\u003e \u003cp\u003e4.2.2 Transfection Agents 73\u003c\/p\u003e \u003cp\u003e4.2.3 Labeling Protocols 75\u003c\/p\u003e \u003cp\u003e4.3 Applications 76\u003c\/p\u003e \u003cp\u003e4.3.1 Traditional Usage of SPIO‐Labeled Cellular MRI 76\u003c\/p\u003e \u003cp\u003e4.3.2 SPIO‐Labeled Cellular MRI in Tissue Engineering 76\u003c\/p\u003e \u003cp\u003e4.4 Case Study: SPIO‐Labeled Cellular MRI for Heart Valve Tissue Engineering 77\u003c\/p\u003e \u003cp\u003e4.4.1 Experimental Design 77\u003c\/p\u003e \u003cp\u003e4.4.2 Potential Approaches—In Vitro 78\u003c\/p\u003e \u003cp\u003e4.4.3 Potential Approaches—In Vivo 81\u003c\/p\u003e \u003cp\u003e4.5 Conclusions and Future Outlook 83\u003c\/p\u003e \u003cp\u003eAcknowledgment 84\u003c\/p\u003e \u003cp\u003eReferences 84\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Magnetic Resonance Elastography Applications in Tissue Engineering 91\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eShadi F. Othman and Richard L. Magin\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction 91\u003c\/p\u003e \u003cp\u003e5.2 Introduction to MRE 93\u003c\/p\u003e \u003cp\u003e5.2.1 Theoretical Basis of MRE 94\u003c\/p\u003e \u003cp\u003e5.2.2 The Inverse Problem and Direct Algebraic Inversion 96\u003c\/p\u003e \u003cp\u003e5.2.3 Direct Algebraic Inversion Algorithm 101\u003c\/p\u003e \u003cp\u003e5.3 Current Applications of MRE in Tissue Engineering and Regenerative Medicine 108\u003c\/p\u003e \u003cp\u003e5.3.1 In Vitro TE μMRE 108\u003c\/p\u003e \u003cp\u003e5.3.2 In Vivo TE μMRE 110\u003c\/p\u003e \u003cp\u003e5.4 Conclusion 114\u003c\/p\u003e \u003cp\u003eReferences 114\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Finite‐Element Method in MR Elastography: Application in Tissue Engineering 117\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eYifei Liu and Thomas J. Royston\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction 117\u003c\/p\u003e \u003cp\u003e6.2 FEA in MRE Inversion Algorithm Verification 118\u003c\/p\u003e \u003cp\u003e6.3 FEM in Stiffness Estimation from MRE Data 120\u003c\/p\u003e \u003cp\u003e6.4 FEA in Experimental Validation in Tissue Engineering Application 121\u003c\/p\u003e \u003cp\u003e6.5 Conclusions and Discussion 124\u003c\/p\u003e \u003cp\u003eAcknowledgment 125\u003c\/p\u003e \u003cp\u003eReferences 125\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 In Vivo EPR Oxygen Imaging: A Case for Tissue Engineering 129\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eBoris Epel, Mrignayani Kotecha, and Howard J. Halpern\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction 129\u003c\/p\u003e \u003cp\u003e7.2 History of EPROI 131\u003c\/p\u003e \u003cp\u003e7.3 Principles of EPR Imaging 132\u003c\/p\u003e \u003cp\u003e7.4 EPR Oxymetry 134\u003c\/p\u003e \u003cp\u003e7.5 EPROI Instrumentation and Methodology 135\u003c\/p\u003e \u003cp\u003e7.5.1 EPR Frequency 135\u003c\/p\u003e \u003cp\u003e7.5.2 Resonators 135\u003c\/p\u003e \u003cp\u003e7.5.3 Magnets 136\u003c\/p\u003e \u003cp\u003e7.5.4 EPR Imagers 137\u003c\/p\u003e \u003cp\u003e7.6 Spin Probes for Pulse EPR Oxymetry 138\u003c\/p\u003e \u003cp\u003e7.7 Image Registration 139\u003c\/p\u003e \u003cp\u003e7.8 Tissue Engineering Applications 140\u003c\/p\u003e \u003cp\u003e7.8.1 EPROI in Scaffold Design 140\u003c\/p\u003e \u003cp\u003e7.8.2 EPROI in Tissue Engineering 142\u003c\/p\u003e \u003cp\u003e7.9 Summary and Future Outlook 142\u003c\/p\u003e \u003cp\u003eAcknowledgment 142\u003c\/p\u003e \u003cp\u003eReferences 143\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart II Tissue‐Specific Applications of Magnetic Resonance Imaging in Tissue Engineering 149\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Tissue‐Engineered Grafts for Bone and Meniscus Regeneration and Their Assessment Using MRI 151\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eHanying Bai, Mo Chen, Yongxing Liu, Qimei Gong, Ling He, Juan Zhong, Guodong Yang, Jinxuan Zheng, Xuguang Nie, Yixiong Zhang, and Jeremy J. Mao\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e8.1 Overview of Tissue Engineering with MRI 151\u003c\/p\u003e \u003cp\u003e8.2 Assessment of Bone Regeneration by Tissue Engineering with MRI 152\u003c\/p\u003e \u003cp\u003e8.3 MRI for 3D Modeling and 3D Print Manufacturing in Tissue Engineering 157\u003c\/p\u003e \u003cp\u003e8.4 Assessment of Menisci Repair and Regeneration by Tissue Engineering with MRI 161\u003c\/p\u003e \u003cp\u003e8.5 Conclusion 168\u003c\/p\u003e \u003cp\u003eAcknowledgments 168\u003c\/p\u003e \u003cp\u003eReferences 169\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 MRI Assessment of Engineered Cartilage Tissue Growth 179\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eMrignayani Kotecha and Richard L. Magin\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction 179\u003c\/p\u003e \u003cp\u003e9.2 Cartilage 181\u003c\/p\u003e \u003cp\u003e9.3 Cartilage Tissue Engineering 182\u003c\/p\u003e \u003cp\u003e9.3.1 Cells 183\u003c\/p\u003e \u003cp\u003e9.3.1.1 Chondrocytes 183\u003c\/p\u003e \u003cp\u003e9.3.1.2 Stem Cells 183\u003c\/p\u003e \u003cp\u003e9.3.2 Biomaterials 183\u003c\/p\u003e \u003cp\u003e9.3.3 Growth Factors 184\u003c\/p\u003e \u003cp\u003e9.3.4 Growth Conditions 184\u003c\/p\u003e \u003cp\u003e9.4 Animal Models in Cartilage Tissue Engineering 184\u003c\/p\u003e \u003cp\u003e9.5 Tissue Growth Assessment 186\u003c\/p\u003e \u003cp\u003e9.6 MRI in the Assessment of Tissue‐Engineered Cartilage 187\u003c\/p\u003e \u003cp\u003e9.7 Periodic Assessment of Tissue‐Engineered Cartilage Using MRI 187\u003c\/p\u003e \u003cp\u003e9.7.1 Assessment of Tissue Growth In Vitro 187\u003c\/p\u003e \u003cp\u003e9.7.1.1 Accounting for Scaffold in Tissue Assessment 191\u003c\/p\u003e \u003cp\u003e9.7.2 Assessment of Tissue Growth In Vivo 191\u003c\/p\u003e \u003cp\u003e9.7.3 Assessment of Tissue Anisotropy and Dynamics 193\u003c\/p\u003e \u003cp\u003e9.7.3.1 Assessment of Macromolecule Composition 194\u003c\/p\u003e \u003cp\u003e9.7.3.2 Assessment of Tissue Anisotropy 198\u003c\/p\u003e \u003cp\u003e9.8 Summary and Future Directions 199\u003c\/p\u003e \u003cp\u003eReferences 200\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Emerging Techniques for Tendon and Ligament MRI 209\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eBraden C. Fleming, Alison M. Biercevicz, Martha M. Murray, Weiguo Li, and Vincent M. Wang\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e10.1 Tendon and Ligament Structure, Function, Injury, and Healing 209\u003c\/p\u003e \u003cp\u003e10.2 MRI Studies of Tendon and Ligament Healing 211\u003c\/p\u003e \u003cp\u003e10.3 MRI and Contrast Mechanisms 219\u003c\/p\u003e \u003cp\u003e10.3.1 Conventional MRI Techniques 219\u003c\/p\u003e \u003cp\u003e10.3.2 Advanced MR Techniques 222\u003c\/p\u003e \u003cp\u003e10.4 Significance and Conclusion 228\u003c\/p\u003e \u003cp\u003eAcknowledgments 228\u003c\/p\u003e \u003cp\u003eReferences 228\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 MRI of Engineered Dental and Craniofacial Tissues 237\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eAnne George and Sriram Ravindran\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e11.1 Introduction 237\u003c\/p\u003e \u003cp\u003e11.2 Scaffolds 238\u003c\/p\u003e \u003cp\u003e11.3 Extracellular Matrix 238\u003c\/p\u003e \u003cp\u003e11.4 Tissue Regeneration of Dental–Craniofacial Complex 239\u003c\/p\u003e \u003cp\u003e11.4.1 Advantages of Using ECM Scaffolds with Stem Cells 240\u003c\/p\u003e \u003cp\u003e11.4.2 Stem Cells 242\u003c\/p\u003e \u003cp\u003e11.5 MRI in Tissue Engineering and Regeneration 243\u003c\/p\u003e \u003cp\u003e11.5.1 MRI of Human DPSCs 243\u003c\/p\u003e \u003cp\u003e11.5.2 MRI of Tissue‐Engineered Osteogenic Scaffolds 244\u003c\/p\u003e \u003cp\u003e11.5.3 MRI of Chondrogenic Scaffolds with Cells In Vitro 244\u003c\/p\u003e \u003cp\u003e11.5.4 MRI of Chondrogenic Scaffolds with Cells In Vivo 245\u003c\/p\u003e \u003cp\u003e11.5.5 MRI Can Differentiate Between Engineered Bone and Engineered Cartilage 246\u003c\/p\u003e \u003cp\u003e11.5.6 MRI to Assess Angiogenesis 246\u003c\/p\u003e \u003cp\u003e11.6 Challenges and Future Directions for MRI in Tissue Engineering 246\u003c\/p\u003e \u003cp\u003eAcknowledgments 247\u003c\/p\u003e \u003cp\u003eReferences 247\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Osteochondral Tissue Engineering: Noninvasive Assessment of Tissue Regeneration 251\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eTyler Stahl, Abeid Anslip, Ling Lei, Nilse Dos Santos, Emmanuel Nwachuku, Thomas DeBerardino, and Syam Nukavarapu\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction 251\u003c\/p\u003e \u003cp\u003e12.2 Osteochondral Tissue Engineering 252\u003c\/p\u003e \u003cp\u003e12.2.1 Osteochondral Tissue 252\u003c\/p\u003e \u003cp\u003e12.2.2 Biomaterials\/Scaffolds 252\u003c\/p\u003e \u003cp\u003e12.2.3 Cells 255\u003c\/p\u003e \u003cp\u003e12.2.4 Growth Factors 256\u003c\/p\u003e \u003cp\u003e12.3 Clinical Methods for Osteochondral Defect Repair and Assessment 257\u003c\/p\u003e \u003cp\u003e12.3.1 Diagnostic Modalities 257\u003c\/p\u003e \u003cp\u003e12.3.2 Treatment Methods 260\u003c\/p\u003e \u003cp\u003e12.3.2.1 Microfracture 260\u003c\/p\u003e \u003cp\u003e12.3.2.2 Autografts and Allografts 260\u003c\/p\u003e \u003cp\u003e12.3.2.3 Tissue Engineering Grafts 262\u003c\/p\u003e \u003cp\u003e12.4 MRI Assessment of Tissue Engineered Osteochondral Grafts 262\u003c\/p\u003e \u003cp\u003e12.4.1 In Vitro Assessment 263\u003c\/p\u003e \u003cp\u003e12.4.2 In Vivo Assessment 264\u003c\/p\u003e \u003cp\u003e12.5 MRI Assessment Correlation with Histology 264\u003c\/p\u003e \u003cp\u003e12.6 Conclusions and Challenges 265\u003c\/p\u003e \u003cp\u003eAcknowledgments 265\u003c\/p\u003e \u003cp\u003eReferences 265\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Advanced Liver Tissue Engineering Approaches and Their Measure of Success Using NMR\/MRI 273\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eHaakil Lee, Rex M. Jeffries, Andrey P. Tikunov, and Jeffrey M. Macdonald\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e13.1 Introduction 273\u003c\/p\u003e \u003cp\u003e13.2 MRS and MRI Compatibilization—Building Compact RF MR Probes for BALs 278\u003c\/p\u003e \u003cp\u003e13.3 Multinuclear MRS of a Hybrid Hollow Fiber–Microcarrier BAL 280\u003c\/p\u003e \u003cp\u003e13.3.1 Viability by 31P MRS 282\u003c\/p\u003e \u003cp\u003e13.3.2 Quantifying Drug Metabolic Activity and Oxygen Distribution by 19F MRS 284\u003c\/p\u003e \u003cp\u003e13.4 1H MRI of a Hollow Fiber Multicoaxial BAL 286\u003c\/p\u003e \u003cp\u003e13.4.1 BAL Integrity and Quality Assurance 286\u003c\/p\u003e \u003cp\u003e13.4.2 Inoculation Efficiency and Prototype Redesign Iteration 288\u003c\/p\u003e \u003cp\u003e13.4.3 Flow Dynamics 289\u003c\/p\u003e \u003cp\u003e13.4.4 Diffusion‐Weighted and Functional Annotation Screening Technology (FAST) Dynamic Contrast MRI 291\u003c\/p\u003e \u003cp\u003e13.5 Magnetic Contrast Agents Used in MRI of Liver Stem Cell Therapy 293\u003c\/p\u003e \u003cp\u003e13.6 31P and 13C MRS of a Fluidized‐Bed BAL Containing Encapsulated Hepatocytes 294\u003c\/p\u003e \u003cp\u003e13.6.1 31P MRS Resolution, SNR, Viability, and pH 296\u003c\/p\u003e \u003cp\u003e13.6.2 13C MRS to Monitor Real‐Time Metabolism 296\u003c\/p\u003e \u003cp\u003e13.7 Future Studies 298\u003c\/p\u003e \u003cp\u003e13.7.1 Dynamic Nuclear Polarization 298\u003c\/p\u003e \u003cp\u003e13.7.2 Constructing Artificial Organs 300\u003c\/p\u003e \u003cp\u003e13.8 Discussion 301\u003c\/p\u003e \u003cp\u003eAcknowledgment 303\u003c\/p\u003e \u003cp\u003eReferences 303\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 MRI of Vascularized Tissue‐Engineered Organs 311\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eHai‐Ling Margaret Cheng\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e14.1 Introduction 311\u003c\/p\u003e \u003cp\u003e14.2 Importance of Vascularization in Tissue Engineering 312\u003c\/p\u003e \u003cp\u003e14.3 Vessel Formation and Maturation: Implications for Imaging 314\u003c\/p\u003e \u003cp\u003e14.4 Imaging Approaches to Assess Vascularization 317\u003c\/p\u003e \u003cp\u003e14.5 Dynamic Contrast‐Enhanced MRI for Imaging Vascular Physiology 318\u003c\/p\u003e \u003cp\u003e14.6 Complementary MRI Techniques to Study Vascularization 321\u003c\/p\u003e \u003cp\u003e14.7 Considerations for Preclinical Models and Translation to Clinical Implementation 325\u003c\/p\u003e \u003cp\u003e14.8 Future Directions 326\u003c\/p\u003e \u003cp\u003e14.9 Conclusions 327\u003c\/p\u003e \u003cp\u003eReferences 327\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 MRI Tools for Assessment of Cardiovascular Tissue Engineering 333\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eLaurence H. Jackson, Mark F. Lythgoe, and Daniel J. Stuckey\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e15.1 The Heart and Heart Failure 333\u003c\/p\u003e \u003cp\u003e15.2 Cardiac Engineering and Cell Therapy 334\u003c\/p\u003e \u003cp\u003e15.3 Imaging Heart Failure 336\u003c\/p\u003e \u003cp\u003e15.3.1 Cine MRI 336\u003c\/p\u003e \u003cp\u003e15.3.2 Regional Heart Function 338\u003c\/p\u003e \u003cp\u003e15.3.3 Viability Imaging 340\u003c\/p\u003e \u003cp\u003e15.3.4 Relaxometry and Parametric Imaging 342\u003c\/p\u003e \u003cp\u003e15.3.5 Myocardial Perfusion Imaging 344\u003c\/p\u003e \u003cp\u003e15.4 Imaging Cardiac Regeneration 346\u003c\/p\u003e \u003cp\u003e15.5 Monitoring Cardiac Regeneration 348\u003c\/p\u003e \u003cp\u003e15.5.1 MRI to Track Stem Cells 348\u003c\/p\u003e \u003cp\u003e15.5.2 MRI to Track Engineered Tissues 353\u003c\/p\u003e \u003cp\u003e15.6 Translational Potential and Future Directions 355\u003c\/p\u003e \u003cp\u003eReferences 357\u003c\/p\u003e \u003cp\u003e\u003cb\u003e16 Peripheral Nerve Tissue Engineering and Regeneration Observed Using MRI 367\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eShan‐Ho Chan and Shan‐hui Hsu\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e16.1 Introduction 367\u003c\/p\u003e \u003cp\u003e16.2 Receiver Coils Commonly Applied in Nerve Tissue Engineering 368\u003c\/p\u003e \u003cp\u003e16.3 Various Tools for Real‐Time Monitoring of the Nerve Regeneration 368\u003c\/p\u003e \u003cp\u003e16.4 Current Materials, Methods, and Concepts in Peripheral Nerve Repair 368\u003c\/p\u003e \u003cp\u003e16.5 MRI Parameters in Peripheral Nerve Tissue Engineering 371\u003c\/p\u003e \u003cp\u003e16.6 Advantages of Real‐Time Monitoring of Nerve Regeneration Using MRI 373\u003c\/p\u003e \u003cp\u003e16.7 Choosing Animal Models for MRI Studies of Peripheral Nerve Tissue Engineering 374\u003c\/p\u003e \u003cp\u003e16.8 Imaging Ability Through Nerve Conduits of Peripheral Nerve Tissue Engineering 375\u003c\/p\u003e \u003cp\u003e16.9 Further Imaging Functions of MRI in Peripheral Nerve Tissue Engineering 376\u003c\/p\u003e \u003cp\u003e16.10 Tractography in Peripheral Nerve Tissue Engineering 376\u003c\/p\u003e \u003cp\u003e16.11 Novel Contrast Agents 378\u003c\/p\u003e \u003cp\u003e16.12 Conclusions 378\u003c\/p\u003e \u003cp\u003eReferences 379\u003c\/p\u003e \u003cp\u003eIndex 383\u003c\/p\u003e   \u003cp\u003e\u003cb\u003e MRIGNAYANI KOTECHA\u003c\/b\u003e is currently a research professor of bioengineering at University of Illinois at Chicago and directs the Biomolecular Magnetic Resonance Spectroscopy and Imaging Laboratory (BMRSI). In this position, she is developing proton and sodium magnetic resonance spectroscopy (MRS) and magnetic resonance imaging (MRI) techniques for monitoring the growth of musculoskeletal engineered tissues. Her broad research interests include the application of MRI-based techniques to cell and tissue-based regenerative medicine.   \u003c\/p\u003e\u003cp\u003e\u003cb\u003e RICHARD L. MAGIN\u003c\/b\u003e is currently a professor of bioengineering at University of Illinois at Chicago and directs the Diagnostic NMR Systems Laboratory, USA. Professor Magin is a fellow of the IEEE and AIMBE and a former editor of \u003ci\u003eCritical Reviews in Biomedical Engineering.\u003c\/i\u003e In 2012 he was designated a \"Distinguished\" Professor of Bioengineering at UIC. His research interests focus on the applications of magnetic resonance imaging (MRI) in science and engineering.   \u003c\/p\u003e\u003cp\u003e\u003cb\u003e JEREMY J. MAO\u003c\/b\u003e is currently professor at Columbia University, USA, and also Edwin S. Robinson Endowed Chair. Dr. Mao's research team has been at Columbia for the past 7 years and made several important discoveries including a cover article in The Lancet. In addition, Dr. Mao's work has been published in \u003ci\u003eNature Medicine, The Lancet, Cell Stem Cell, JCI,\u003c\/i\u003e and so on. Altogether Dr. Mao has published over 260 scientific papers and proceedings and written 2 books. Dr. Mao's research has led to over 70 patents and establishment of 2 biotechnology companies. Dr. Mao has received a number of prestigious awards including Yasuda Award and Spanadel Award.     \u003c\/p\u003e\u003cp\u003e\u003cb\u003e Provides a unique overview of the field of noninvasive magnetic resonance assessment of tissue engineering and regenerative medicine \u003c\/b\u003e  \u003c\/p\u003e\u003cp\u003e Groundbreaking work in tissue-engineering and regenerative medicine is paving the way for repairing and\/or replacing biological tissues and organs. Engineers, scientists, and physicians work together in this field to use biomaterials and cells as the building blocks for new life. One of the newest and most exciting areas of tissue engineering is the incorporation of bio-inspired cues into scaffolds through techniques such as patterning of topographies and adhesive ligands. These cues can influence cellular functions, including differentiation, in new ways that are being discovered every day and are paving the way for cutting-edge clinical breakthroughs in areas such as cardiovascular tissue engineering, bone repair, and organ regeneration.   \u003c\/p\u003e\u003cp\u003e\u003ci\u003e Magnetic Resonance Imaging in Tissue Engineering\u003c\/i\u003e is designed to provide in-depth analysis of advances made in the MRI assessment of tissue-engineering applications during the last decade. The book features:   \u003c\/p\u003e\u003cul\u003e \u003cli\u003eComprehensive details of magnetic resonance imaging (MRI) techniques used to assess a variety of engineered and regenerating tissues and organs\u003c\/li\u003e \u003cli\u003eNew techniques to assess engineered tissues\u003c\/li\u003e \u003cli\u003eCell-based therapies and engineered cartilage, bone, musculoskeletal, tendon, ligament, dental, craniofacial, cardiovascular, liver, bladder, and peripheral nerve tissue engineering and regeneration assessed by MRI\u003c\/li\u003e \u003c\/ul\u003e \u003cbr\u003e  \u003cp\u003e\u003ci\u003e Magnetic Resonance Imaging in Tissue Engineering\u003c\/i\u003e contains chapters from leading experts working to develop robust functional engineered tissues and from analytical experts developing new magnetic resonance imaging techniques to assess engineered tissues. This rare interdisciplinary project is developed to bridge a gap in current offerings. Readers from both tissue-engineering and imaging backgrounds will find this interdisciplinary material useful in their research.\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47989552251109,"sku":"NP9781119193357","price":218.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9781119193357.jpg?v=1761784567","url":"https:\/\/k12savings.com\/es\/products\/magnetic-resonance-imaging-in-tissue-engineering-isbn-9781119193357","provider":"K12savings","version":"1.0","type":"link"}