{"product_id":"larones-medically-important-fungi-isbn-9781683674405","title":"Larone's Medically Important Fungi","description":"\u003cb\u003e Larone’s\u003c\/b\u003e \u003cb\u003eMEDICALLY IMPORTANT FUNGI\u003c\/b\u003e \u003cp\u003e\u003cb\u003eThe definitive guide for identifying fungi from clinical specimens\u003c\/b\u003e \u003c\/p\u003e\u003cp\u003eWith a new team of authors, \u003ci\u003eLarone’s Medically Important Fungi\u003c\/i\u003e, Seventh Edition, continues the longstanding tradition of high-quality content to expand your knowledge and support your work in clinical mycology by: \u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eProviding detailed descriptions of the major mycoses as viewed in patients’ specimens by direct microscopic examination of stained slides\u003c\/li\u003e\n\u003cli\u003eOffering a logical step-by-step process for identification of cultured organisms, utilizing detailed descriptions, images, pointers on organisms’ similarities and distinctions, and selected references for further information\u003c\/li\u003e\n\u003cli\u003eCovering more than 150 of the fungi most commonly encountered in the clinical mycology laboratory, including new entries for \u003ci\u003eEmergomyces, Metarhizium anisopliae\u003c\/i\u003e, \u003ci\u003eRasamsonia argillacea, Rhinocladiella mackenziei, Schizophyllum commune, \u003c\/i\u003eand \u003ci\u003eThermothelomyces thermophilus\u003c\/i\u003e\n\u003c\/li\u003e\n\u003cli\u003ePresenting details on each organism’s pathogenicity, growth characteristics, relevant biochemical reactions, and microscopic morphology, illustrated with photomicrographs, unique and elegant drawings, and color photos of colony morphology and various test results\u003c\/li\u003e\n\u003cli\u003eExplaining changes in fungal taxonomy and nomenclature that are due to information acquired through molecular taxonomic studies of evolutionary fungal relationships\u003c\/li\u003e\n\u003cli\u003eProviding basic information on molecular diagnostic methods, e.g., nucleic acid amplification and sequencing, MALDI-TOF mass spectrometry, and other commercial platforms\u003c\/li\u003e\n\u003cli\u003eIncluding an extensive section of easy-to-follow lab protocols, a comprehensive list of media and stain procedures, guidance on collection and preparation of patient specimens, and an illustrated glossary\u003c\/li\u003e\n\u003c\/ul\u003e \u003cp\u003eWith \u003ci\u003eLarone’s Medically Important Fungi: A Guide to Identification\u003c\/i\u003e, both novices and experienced professionals in clinical microbiology laboratories can confidently identify commonly encountered fungi. \u003c\/p\u003e\u003cp\u003eList of Tables xvii\u003c\/p\u003e \u003cp\u003ePreface to the Seventh Edition xix\u003c\/p\u003e \u003cp\u003ePreface to the First Edition xxi\u003c\/p\u003e \u003cp\u003eAcknowledgments xxiii\u003c\/p\u003e \u003cp\u003eAbout the Authors xxv\u003c\/p\u003e \u003cp\u003e\u003cb\u003eBasics 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eHow To Use the Guide 3\u003c\/p\u003e \u003cp\u003eUse of Reference Laboratories and Regulations for Transport 5\u003c\/p\u003e \u003cp\u003eSafety Precautions 11\u003c\/p\u003e \u003cp\u003eTaxonomy and Nomenclature 13\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart I Direct Microscopic Examination of Clinical Specimens 15\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 17\u003c\/p\u003e \u003cp\u003eHistological Terminology 21\u003c\/p\u003e \u003cp\u003eTissue Reactions to Fungal Infection 25\u003c\/p\u003e \u003cp\u003eStains 29\u003c\/p\u003e \u003cp\u003eTable 1.1 Histochemical stains for fungi and\/or filamentous bacteria in tissue 30\u003c\/p\u003e \u003cp\u003eGuide to Interpretation of Direct Microscopic Examination 33\u003c\/p\u003e \u003cp\u003eDetailed Descriptions 39\u003c\/p\u003e \u003cp\u003eActinomycosis 40\u003c\/p\u003e \u003cp\u003eMycetoma (Actinomycotic or Eumycotic) 41\u003c\/p\u003e \u003cp\u003eNocardiosis 43\u003c\/p\u003e \u003cp\u003eMucormycosis (Zygomycosis) 44\u003c\/p\u003e \u003cp\u003eAspergillosis 45\u003c\/p\u003e \u003cp\u003eMiscellaneous Hyalohyphomycoses (Other than Aspergillosis) 47\u003c\/p\u003e \u003cp\u003eDermatophytosis (Tinea, Ringworm) 49\u003c\/p\u003e \u003cp\u003eTinea versicolor 50\u003c\/p\u003e \u003cp\u003eTinea nigra 51\u003c\/p\u003e \u003cp\u003ePhaeohyphomycosis 52\u003c\/p\u003e \u003cp\u003eChromoblastomycosis 53\u003c\/p\u003e \u003cp\u003eSporotrichosis 54\u003c\/p\u003e \u003cp\u003eHistoplasmosis 55\u003c\/p\u003e \u003cp\u003eEmergomycosis 57\u003c\/p\u003e \u003cp\u003eTalaromycosis (Penicilliosis) 58\u003c\/p\u003e \u003cp\u003eBlastomycosis 59\u003c\/p\u003e \u003cp\u003eParacoccidioidomycosis 60\u003c\/p\u003e \u003cp\u003eLobomycosis 61\u003c\/p\u003e \u003cp\u003eCandidiasis 62\u003c\/p\u003e \u003cp\u003eTrichosporonosis 64\u003c\/p\u003e \u003cp\u003eCryptococcosis 65\u003c\/p\u003e \u003cp\u003ePneumocystosis 67\u003c\/p\u003e \u003cp\u003eProtothecosis 68\u003c\/p\u003e \u003cp\u003eCoccidioidomycosis 69\u003c\/p\u003e \u003cp\u003eRhinosporidiosis 70\u003c\/p\u003e \u003cp\u003eAdiaspiromycosis 72\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart II Identification of Fungi in Culture 73\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eGuide to Identification of Fungi in Culture 75\u003c\/p\u003e \u003cp\u003eDetailed Descriptions 105\u003c\/p\u003e \u003cp\u003e\u003cb\u003eFilamentous Bacteria 107\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction to Filamentous Bacteria 109\u003c\/p\u003e \u003cp\u003eTable 2.1 Differentiation of filamentous aerobic actinomycetes encountered in clinical specimens 110\u003c\/p\u003e \u003cp\u003eNocardia spp. 111\u003c\/p\u003e \u003cp\u003eStreptomyces spp. 114\u003c\/p\u003e \u003cp\u003eActinomadura spp. 116\u003c\/p\u003e \u003cp\u003eNocardiopsis dassonvillei 117\u003c\/p\u003e \u003cp\u003e\u003cb\u003eYeasts and Yeastlike Organisms 119\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction to Yeasts and Yeastlike Organisms 121\u003c\/p\u003e \u003cp\u003eCandida albicans 123\u003c\/p\u003e \u003cp\u003eTable 2.2 Characteristics of the genera of clinically encountered yeasts and yeastlike organisms 124\u003c\/p\u003e \u003cp\u003eCandida dubliniensis 125\u003c\/p\u003e \u003cp\u003eTable 2.3 Characteristics of Candida spp. most commonly encountered in the clinical laboratory 126\u003c\/p\u003e \u003cp\u003eTable 2.4 Characteristics that assist in differentiating Candida dubliniensis from Candida albicans 128\u003c\/p\u003e \u003cp\u003eCandida tropicalis 129\u003c\/p\u003e \u003cp\u003eCandida parapsilosis species complex 130\u003c\/p\u003e \u003cp\u003eCandida lusitaniae 131\u003c\/p\u003e \u003cp\u003eCandida krusei 132\u003c\/p\u003e \u003cp\u003eTable 2.5 Differentiating characteristics of Magnusiomyces capitatus (formerly Blastoschizomyces capitatus) versus Candida krusei 134\u003c\/p\u003e \u003cp\u003eTable 2.6 Differentiating characteristics of Candida krusei, Candida inconspicua, and Candida norvegensis 134\u003c\/p\u003e \u003cp\u003eCandida kefyr 135\u003c\/p\u003e \u003cp\u003eCandida rugosa species complex 136\u003c\/p\u003e \u003cp\u003eCandida guilliermondii species complex 138\u003c\/p\u003e \u003cp\u003eTable 2.7 Differentiating characteristics of Candida guilliermondii versus Candida famata 139\u003c\/p\u003e \u003cp\u003eCandida lipolytica 140\u003c\/p\u003e \u003cp\u003eCandida zeylanoides 141\u003c\/p\u003e \u003cp\u003eCandida glabrata species complex 142\u003c\/p\u003e \u003cp\u003eCandida auris 143\u003c\/p\u003e \u003cp\u003eCandida haemulonii species complex 144\u003c\/p\u003e \u003cp\u003eCandida pelliculosa 145\u003c\/p\u003e \u003cp\u003eCryptococcus neoformans species complex 147\u003c\/p\u003e \u003cp\u003eCryptococcus gattii species complex 149\u003c\/p\u003e \u003cp\u003eTable 2.8 Characteristics of Cryptococcus spp. and former members of the genus 150\u003c\/p\u003e \u003cp\u003eTable 2.9 Characteristics of yeasts and yeastlike organisms other than Candida spp. and Cryptococcus spp. 151\u003c\/p\u003e \u003cp\u003eRhodotorula and Cystobasidium spp. 152\u003c\/p\u003e \u003cp\u003eSporobolomyces salmonicolor 154\u003c\/p\u003e \u003cp\u003eSaccharomyces cerevisiae 156\u003c\/p\u003e \u003cp\u003eMalassezia spp. 158\u003c\/p\u003e \u003cp\u003eMalassezia pachydermatis 160\u003c\/p\u003e \u003cp\u003eUstilago spp. 161\u003c\/p\u003e \u003cp\u003ePrototheca spp. 162\u003c\/p\u003e \u003cp\u003eTrichosporon and Cutaneotrichosporon spp. 163\u003c\/p\u003e \u003cp\u003eTable 2.10 Key characteristics of the most common clinically encountered Trichosporon spp. and Cutaneotrichosporon spp. 165\u003c\/p\u003e \u003cp\u003eMagnusiomyces capitatus (formerly Blastoschizomyces capitatus) 166\u003c\/p\u003e \u003cp\u003eGeotrichum candidum 167\u003c\/p\u003e \u003cp\u003e\u003cb\u003eThermally Dimorphic and\/or Endemic Fungi 169\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction to Thermally Dimorphic and\/or Endemic Fungi 171\u003c\/p\u003e \u003cp\u003eHistoplasma capsulatum 172\u003c\/p\u003e \u003cp\u003eEmergomyces spp. 175\u003c\/p\u003e \u003cp\u003eBlastomyces dermatitidis\/gilchristii 177\u003c\/p\u003e \u003cp\u003eCoccidioides immitis\/posadasii 179\u003c\/p\u003e \u003cp\u003eParacoccidioides brasiliensis 181\u003c\/p\u003e \u003cp\u003eTalaromyces marneffei (formerly Penicillium marneffei) 183\u003c\/p\u003e \u003cp\u003eSporothrix schenckii species complex 186\u003c\/p\u003e \u003cp\u003eEmmonsia crescens 189\u003c\/p\u003e \u003cp\u003eThermally Monomorphic Moulds 191\u003c\/p\u003e \u003cp\u003e\u003cb\u003eMucormycetes 193\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction to Mucormycetes 195\u003c\/p\u003e \u003cp\u003eTable 2.11 Differential characteristics of similar organisms in the class Mucormycetes 197\u003c\/p\u003e \u003cp\u003eTable 2.12 Differential characteristics of the clinically encountered Rhizopus spp. 197\u003c\/p\u003e \u003cp\u003eRhizopus spp. 198\u003c\/p\u003e \u003cp\u003eMucor spp. 200\u003c\/p\u003e \u003cp\u003eRhizomucor spp. 201\u003c\/p\u003e \u003cp\u003eLichtheimia corymbifera species complex 202\u003c\/p\u003e \u003cp\u003eApophysomyces elegans species complex 204\u003c\/p\u003e \u003cp\u003eSaksenaea vasiformis 206\u003c\/p\u003e \u003cp\u003eCokeromyces recurvatus 207\u003c\/p\u003e \u003cp\u003eCunninghamella bertholletiae 209\u003c\/p\u003e \u003cp\u003eSyncephalastrum racemosum 211\u003c\/p\u003e \u003cp\u003eBasidiobolus spp. 212\u003c\/p\u003e \u003cp\u003eConidiobolus coronatus 213\u003c\/p\u003e \u003cp\u003e\u003cb\u003eDematiaceous Fungi 215\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction to Dematiaceous Fungi 217\u003c\/p\u003e \u003cp\u003eFonsecaea spp. 218\u003c\/p\u003e \u003cp\u003eMyrmecridium schulzeri 221\u003c\/p\u003e \u003cp\u003eRhinocladiella mackenziei (formerly Ramichloridium mackenziei) 222\u003c\/p\u003e \u003cp\u003ePhialophora verrucosa 223\u003c\/p\u003e \u003cp\u003eTable 2.13 Characteristics of Phialophora, Pleurostoma (formerly Pleurostomophora), Phaeoacremonium, Acremonium and Sarocladium, Phialemonium, and Coniochaeta (formerly Lecythophora) 224\u003c\/p\u003e \u003cp\u003ePleurostoma richardsiae (formerly Pleurostomophora richardsiae) 225\u003c\/p\u003e \u003cp\u003ePhaeoacremonium parasiticum 226\u003c\/p\u003e \u003cp\u003ePhialemonium spp. 228\u003c\/p\u003e \u003cp\u003eCladosporium spp. 230\u003c\/p\u003e \u003cp\u003eTable 2.14 Characteristics of Cladosporium spp. and Cladophialophora spp. 232\u003c\/p\u003e \u003cp\u003eCladophialophora carrionii 233\u003c\/p\u003e \u003cp\u003eCladophialophora boppii 235\u003c\/p\u003e \u003cp\u003eCladophialophora bantiana 236\u003c\/p\u003e \u003cp\u003eScedosporium apiospermum species complex 237\u003c\/p\u003e \u003cp\u003eTable 2.15 Differentiating phenotypic characteristics of the clinically encountered members of Scedosporium spp. and Lomentospora prolificans 241\u003c\/p\u003e \u003cp\u003eLomentospora prolificans (formerly Scedosporium prolificans) 242\u003c\/p\u003e \u003cp\u003eVerruconis gallopava (formerly Ochroconis gallopava) 244\u003c\/p\u003e \u003cp\u003eTable 2.16 Characteristics of some of the “black yeasts” 246\u003c\/p\u003e \u003cp\u003eExophiala jeanselmei species complex 247\u003c\/p\u003e \u003cp\u003eExophiala dermatitidis 249\u003c\/p\u003e \u003cp\u003eHortaea werneckii 251\u003c\/p\u003e \u003cp\u003eMadurella mycetomatis 252\u003c\/p\u003e \u003cp\u003eTrematosphaeria grisea (formerly Madurella grisea) 253\u003c\/p\u003e \u003cp\u003ePiedraia hortae 254\u003c\/p\u003e \u003cp\u003eAureobasidium pullulans 255\u003c\/p\u003e \u003cp\u003eTable 2.17 Differential characteristics of Aureobasidium pullulans versus Hormonema dematioides 257\u003c\/p\u003e \u003cp\u003eHormonema dematioides 258\u003c\/p\u003e \u003cp\u003eNeoscytalidium dimidiatum 259\u003c\/p\u003e \u003cp\u003eBotrytis cinerea 261\u003c\/p\u003e \u003cp\u003eStachybotrys chartarum 262\u003c\/p\u003e \u003cp\u003eThermothelomyces thermophilus (formerly Myceliophthora thermophila) 264\u003c\/p\u003e \u003cp\u003eCurvularia spp. 265\u003c\/p\u003e \u003cp\u003eTable 2.18 Characteristics of Curvularia spp. and Exserohilum rostratum 269\u003c\/p\u003e \u003cp\u003eExserohilum rostratum 270\u003c\/p\u003e \u003cp\u003eHelminthosporium spp. 272\u003c\/p\u003e \u003cp\u003eAlternaria spp. 273\u003c\/p\u003e \u003cp\u003eStemphylium spp. 275\u003c\/p\u003e \u003cp\u003ePseudopithomyces spp. (formerly Pithomyces spp.) 276\u003c\/p\u003e \u003cp\u003eEpicoccum spp. 277\u003c\/p\u003e \u003cp\u003eNigrospora spp. 279\u003c\/p\u003e \u003cp\u003eChaetomium spp. 280\u003c\/p\u003e \u003cp\u003ePhoma spp. 282\u003c\/p\u003e \u003cp\u003e\u003cb\u003eDermatophytes 285\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction to Dermatophytes 287\u003c\/p\u003e \u003cp\u003eLatin Terms for Dermatophyte Infections 288\u003c\/p\u003e \u003cp\u003eMicrosporum audouinii 289\u003c\/p\u003e \u003cp\u003eMicrosporum canis 290\u003c\/p\u003e \u003cp\u003eParaphyton cookei species complex (formerly Microsporum cookei species complex) 292\u003c\/p\u003e \u003cp\u003eNannizzia gypsea species complex (formerly Microsporum gypseum species complex) 293\u003c\/p\u003e \u003cp\u003eLophophyton gallinae (formerly Microsporum gallinae [zoophilic form] and Microsporum vanbreuseghemii [geophilic form]) 295\u003c\/p\u003e \u003cp\u003eNannizzia nana (formerly Microsporum nanum) 297\u003c\/p\u003e \u003cp\u003eMicrosporum ferrugineum 299\u003c\/p\u003e \u003cp\u003eTrichophyton mentagrophytes species complex 300\u003c\/p\u003e \u003cp\u003eTable 2.19 Differentiation of similar conidia-producing Trichophyton spp. and Arthroderma spp. 302\u003c\/p\u003e \u003cp\u003eTrichophyton rubrum 303\u003c\/p\u003e \u003cp\u003eTrichophyton tonsurans 305\u003c\/p\u003e \u003cp\u003eArthroderma terrestre species complex (formerly Trichophyton terrestre species complex) 307\u003c\/p\u003e \u003cp\u003eTrichophyton megninii 308\u003c\/p\u003e \u003cp\u003eTrichophyton soudanense 309\u003c\/p\u003e \u003cp\u003eTable 2.20 Growth patterns of Trichophyton spp. and Arthroderma spp. on nutritional test media 310\u003c\/p\u003e \u003cp\u003eTrichophyton schoenleinii 311\u003c\/p\u003e \u003cp\u003eTrichophyton verrucosum 312\u003c\/p\u003e \u003cp\u003eTrichophyton violaceum 313\u003c\/p\u003e \u003cp\u003eArthroderma uncinatum (formerly Trichophyton ajelloi) 314\u003c\/p\u003e \u003cp\u003eEpidermophyton floccosum 315\u003c\/p\u003e \u003cp\u003eHyaline Hyphomycetes 317\u003c\/p\u003e \u003cp\u003eIntroduction to Hyaline Hyphomycetes 319\u003c\/p\u003e \u003cp\u003e\u003cb\u003eFungi in Which Arthroconidia Predominate \u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eTable 2.21 Differential characteristics of fungi in which arthroconidia predominate 320\u003c\/p\u003e \u003cp\u003eMalbranchea spp. 321\u003c\/p\u003e \u003cp\u003ePseudogymnoascus pannorum (formerly Geomyces pannorum) 323\u003c\/p\u003e \u003cp\u003eArthrographis kalrae 324\u003c\/p\u003e \u003cp\u003eHormographiella aspergillata 326\u003c\/p\u003e \u003cp\u003e\u003cb\u003eCommon Species of Aspergillus\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eThe genus Aspergillus 327\u003c\/p\u003e \u003cp\u003eTable 2.22 Differentiating characteristics of the most common Aspergillus spp. 329\u003c\/p\u003e \u003cp\u003eAspergillus fumigatus species complex 331\u003c\/p\u003e \u003cp\u003eAspergillus niger species complex 333\u003c\/p\u003e \u003cp\u003eAspergillus flavus species complex 334\u003c\/p\u003e \u003cp\u003eAspergillus versicolor species complex 336\u003c\/p\u003e \u003cp\u003eAspergillus ustus species complex 338\u003c\/p\u003e \u003cp\u003eAspergillus tanneri 340\u003c\/p\u003e \u003cp\u003eAspergillus nidulans species complex 342\u003c\/p\u003e \u003cp\u003eAspergillus glaucus 344\u003c\/p\u003e \u003cp\u003eAspergillus terreus species complex 345\u003c\/p\u003e \u003cp\u003eAspergillus clavatus 347\u003c\/p\u003e \u003cp\u003e\u003cb\u003eOther Common Hyaline Hyphomycetes\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003ePenicillium spp. 348\u003c\/p\u003e \u003cp\u003ePaecilomyces variotii 350\u003c\/p\u003e \u003cp\u003eRasamsonia argillacea species complex (formerly Geosmithia argillacea) 351\u003c\/p\u003e \u003cp\u003ePurpureocillium lilacinum (formerly Paecilomyces lilacinus) 352\u003c\/p\u003e \u003cp\u003eTable 2.23 Differential characteristics of Paecilomyces variotii, Rasamsonia argillacea, and Purpureocillium lilacinum 354\u003c\/p\u003e \u003cp\u003eScopulariopsis spp. 355\u003c\/p\u003e \u003cp\u003eTable 2.24 Differential characteristics of Scopulariopsis brevicaulis versus Scopulariopsis brumptii 357\u003c\/p\u003e \u003cp\u003eGliocladium spp. 358\u003c\/p\u003e \u003cp\u003eTrichoderma spp. 359\u003c\/p\u003e \u003cp\u003eMetarhizium anisopliae species complex 361\u003c\/p\u003e \u003cp\u003eBeauveria bassiana 363\u003c\/p\u003e \u003cp\u003eVerticillium spp. 364\u003c\/p\u003e \u003cp\u003eAcremonium and Sarocladium spp. 365\u003c\/p\u003e \u003cp\u003eFusarium spp. 367\u003c\/p\u003e \u003cp\u003eConiochaeta spp. (formerly Lecythophora spp.) 369\u003c\/p\u003e \u003cp\u003eTrichothecium roseum 370\u003c\/p\u003e \u003cp\u003eChrysosporium spp. 371\u003c\/p\u003e \u003cp\u003eTable 2.25 Differential characteristics of Chrysosporium versus Sporotrichum 373\u003c\/p\u003e \u003cp\u003eSporotrichum pruinosum 374\u003c\/p\u003e \u003cp\u003eSepedonium spp. 376\u003c\/p\u003e \u003cp\u003eChrysonilia sitophila 377\u003c\/p\u003e \u003cp\u003eSchizophyllum commune 378\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart III Basics of Molecular Methods for Fungal Identification 379\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 381\u003c\/p\u003e \u003cp\u003eFungal Targets 383\u003c\/p\u003e \u003cp\u003eTable 3.1 Frequently used fungal molecular targets and primers for sequence-based species identification 385\u003c\/p\u003e \u003cp\u003eTable 3.2 Examples of fungal molecular targets and primers for multilocus sequence-based species identification 386\u003c\/p\u003e \u003cp\u003eClassic Molecular Identification Methods 387\u003c\/p\u003e \u003cp\u003ePolymerase Chain Reaction 387\u003c\/p\u003e \u003cp\u003eNon-Sequencing-Based Identification Methods 389\u003c\/p\u003e \u003cp\u003eMALDI-TOF Mass Spectrometry 389\u003c\/p\u003e \u003cp\u003eSignal Amplification Methods 390\u003c\/p\u003e \u003cp\u003ePNA FISH 390\u003c\/p\u003e \u003cp\u003eNucleic Acid Amplification Methods 390\u003c\/p\u003e \u003cp\u003eT2 Magnetic Resonance 390\u003c\/p\u003e \u003cp\u003eBroad-Panel Molecular Testing and Other Emerging Sample-to-Answer Technologies 391\u003c\/p\u003e \u003cp\u003eSequencing-Based Identification Methods 393\u003c\/p\u003e \u003cp\u003eSanger Sequencing 393\u003c\/p\u003e \u003cp\u003eTable 3.3 Lane construction for traditional bidirectional Sanger sequencing 394\u003c\/p\u003e \u003cp\u003eMassive Parallel or Next-Generation Sequencing 394\u003c\/p\u003e \u003cp\u003eApplications of DNA Sequencing 397\u003c\/p\u003e \u003cp\u003eAccurate Molecular Identification 397\u003c\/p\u003e \u003cp\u003eTable 3.4 Commonly used databases for identification of medically important fungi 398\u003c\/p\u003e \u003cp\u003ePhylogenetic Analysis 399\u003c\/p\u003e \u003cp\u003eOrganism Typing 401\u003c\/p\u003e \u003cp\u003eDetection of Genetic Determinants of Resistance 401\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart IV Laboratory Technique 403\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eLaboratory Procedures 405\u003c\/p\u003e \u003cp\u003eCollection and Preparation of Specimens 406\u003c\/p\u003e \u003cp\u003eTable 4.1 Common clinical sites for laboratory recovery of pathogenic fungi 409\u003c\/p\u003e \u003cp\u003eMethods for Direct Microscopic Examination of Specimens 414\u003c\/p\u003e \u003cp\u003ePrimary Isolation 416\u003c\/p\u003e \u003cp\u003eTable 4.2 Media for primary isolation of fungi 417\u003c\/p\u003e \u003cp\u003eMacroscopic Examination of Cultures 419\u003c\/p\u003e \u003cp\u003eMicroscopic Examination of Growth 419\u003c\/p\u003e \u003cp\u003eProcedure for Identification of Yeasts 421\u003c\/p\u003e \u003cp\u003eIsolation of Yeast When Mixed with Bacteria 424\u003c\/p\u003e \u003cp\u003eGerm Tube Test for the Presumptive Identification of Candida albicans 425\u003c\/p\u003e \u003cp\u003eRapid Enzyme Tests for the Presumptive Identification of Candida albicans 426\u003c\/p\u003e \u003cp\u003eCaffeic Acid Disk Test 426\u003c\/p\u003e \u003cp\u003eOlive Oil Disks for Culturing Malassezia spp. 426\u003c\/p\u003e \u003cp\u003eConversion of Thermally Dimorphic Fungi in Culture 427\u003c\/p\u003e \u003cp\u003eSporulation Inducement Method for Apophysomyces and Saksenaea 428\u003c\/p\u003e \u003cp\u003eIn vitro Hair Perforation Test (for Differentiation of Trichophyton  mentagrophytes and Trichophyton rubrum) 428\u003c\/p\u003e \u003cp\u003eTemperature Tolerance Testing 429\u003c\/p\u003e \u003cp\u003eMaintenance of Stock Fungal Cultures 429\u003c\/p\u003e \u003cp\u003eControlling Mites 430\u003c\/p\u003e \u003cp\u003e\u003cb\u003eStaining Methods 431\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eAcid-Fast Modified Kinyoun Stain for Nocardia spp. 432\u003c\/p\u003e \u003cp\u003eAcid-Fast Stain for Ascospores 433\u003c\/p\u003e \u003cp\u003eAscospore Stain 433\u003c\/p\u003e \u003cp\u003eCalcofluor White Stain 434\u003c\/p\u003e \u003cp\u003eGiemsa Stain 435\u003c\/p\u003e \u003cp\u003eGomori Methenamine Silver (GMS) Stain 435\u003c\/p\u003e \u003cp\u003eGram Stain (Hucker Modification) 438\u003c\/p\u003e \u003cp\u003eLactophenol Cotton Blue 439\u003c\/p\u003e \u003cp\u003eLactophenol Cotton Blue with Polyvinyl Alcohol (PVA) (Huber’s PVA Mounting\u003c\/p\u003e \u003cp\u003eMedium, Modified) 439\u003c\/p\u003e \u003cp\u003eRehydration of Paraffin-Embedded Tissue (Deparaffination) 440\u003c\/p\u003e \u003cp\u003e\u003cb\u003eMedia 441\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003eAscospore Media 443\u003c\/p\u003e \u003cp\u003eAssimilation Media (for Yeasts) 444\u003c\/p\u003e \u003cp\u003eBirdseed Agar (Niger Seed Agar; Staib Agar) 448\u003c\/p\u003e \u003cp\u003eBrain Heart Infusion (BHI) Agar 449\u003c\/p\u003e \u003cp\u003eBuffered Charcoal-Yeast Extract (BCYE) Agar 450\u003c\/p\u003e \u003cp\u003eCanavanine Glycine Bromothymol Blue (CGB) Agar 450\u003c\/p\u003e \u003cp\u003eCasein Agar 451\u003c\/p\u003e \u003cp\u003eCHROMagar Candida Medium 452\u003c\/p\u003e \u003cp\u003eCHROMagar Candida Plus Medium 453\u003c\/p\u003e \u003cp\u003eCHROMID Candida Agar 453\u003c\/p\u003e \u003cp\u003eChromogenic Candida Agar (Brilliance Candida Agar) 454\u003c\/p\u003e \u003cp\u003eCornmeal Agar 454\u003c\/p\u003e \u003cp\u003eDermatophyte Test Medium (DTM) 455\u003c\/p\u003e \u003cp\u003eEsculin Agar 456\u003c\/p\u003e \u003cp\u003eFermentation Broth for Yeasts 456\u003c\/p\u003e \u003cp\u003eInhibitory Mould Agar (IMA) 457\u003c\/p\u003e \u003cp\u003eLeeming-Notman Agar (Modified) 458\u003c\/p\u003e \u003cp\u003eLysozyme Medium 458\u003c\/p\u003e \u003cp\u003eMycosel Agar 459\u003c\/p\u003e \u003cp\u003ePotato Dextrose Agar and Potato Flake Agar 460\u003c\/p\u003e \u003cp\u003eRapid Assimilation of Trehalose (RAT) Broth 460\u003c\/p\u003e \u003cp\u003eSabouraud Brain Heart Infusion Agar (SABHI Agar) 462\u003c\/p\u003e \u003cp\u003eSabouraud Dextrose Agar (SDA) 463\u003c\/p\u003e \u003cp\u003eSabouraud Dextrose Agar with 15% NaCl 464\u003c\/p\u003e \u003cp\u003eSabouraud Dextrose Broth 464\u003c\/p\u003e \u003cp\u003eStarch Hydrolysis Agar 464\u003c\/p\u003e \u003cp\u003eTrichophyton Agars 465\u003c\/p\u003e \u003cp\u003eTyrosine Agar 466\u003c\/p\u003e \u003cp\u003eUrea Agar 467\u003c\/p\u003e \u003cp\u003eWater Agar 467\u003c\/p\u003e \u003cp\u003eImage Appendix 469\u003c\/p\u003e \u003cp\u003eGlossary 477\u003c\/p\u003e \u003cp\u003eReferences Cited 489\u003c\/p\u003e \u003cp\u003eIndex 507\u003c\/p\u003e \u003cp\u003e\"Replete with explanations of basic histological terms and descriptions of fundamental tissue reactions to fungal infection, the book achieves the remarkable feat of being simultaneously concise and complete.\"\u003cbr\u003e—\u003cb\u003e\u003ci\u003eEmerging Infectious Diseases,\u003c\/i\u003e Vol. 30 No. 3, March 2024\u003c\/b\u003e\u003c\/p\u003e  \u003cp\u003e\u003cb\u003eLars F. Westblade, \u003c\/b\u003ePhD, D(ABMM) is the Director of the Clinical Microbiology Service at NewYork-Presbyterian\/Weill Cornell Medical Center and Associate Professor at Weill Cornell Medicine with a primary appointment in the Department of Pathology and Laboratory Medicine and a secondary appointment in the Division of Infectious Diseases, Department of Medicine. He earned his doctoral degree from the University of Birmingham in the United Kingdom, and completed a fellowship in medical and public health laboratory microbiology at Washington University School of Medicine in St. Louis. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eEileen M. Burd, \u003c\/b\u003ePhD, D(ABMM) is the Director of the Clinical Microbiology Laboratory at Emory University Hospital and Professor at Emory University School of Medicine with a primary appointment in the Department of Pathology and Laboratory Medicine and a secondary appointment in the Department of Medicine, Division of Infectious Diseases. She earned her doctoral degree from the Medical College of Wisconsin in Milwaukee and was the Division Head of Microbiology at Henry Ford Hospital in Detroit, Michigan prior to joining the faculty at Emory University in 2007. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eShawn R. Lockhart, \u003c\/b\u003ePhD, D(ABBM) FAAM is the Senior Clinical Laboratory Advisor in the Mycotic Diseases Branch at the Centers for Disease Control and Prevention. He earned his doctoral degree from the University of Kentucky and completed his clinical microbiology fellowship at the University of Iowa Hospitals and Clinics. He directs the CDC training course in mold identification. \u003c\/p\u003e\u003cp\u003e\u003cb\u003eGary W. Procop, \u003c\/b\u003eMD, MS is the CEO of the American Board of Pathology and Professor of Pathology at the Cleveland Clinic Lerner School of Medicine. He remains a Consulting Staff for the Institute of Pathology and Laboratory Medicine, where he served as Medical Director for the Mycology Laboratory for more than two decades. He earned his doctoral degree from the Marshall University School of Medicine. His residency in anatomic and clinical pathology was completed at Duke University and his medical microbiology fellowship at the Mayo Clinic.   \u003c\/p\u003e\u003cp\u003e\u003cb\u003eThe definitive guide for identifying fungi from clinical specimens\u003c\/b\u003e \u003c\/p\u003e\u003cp\u003eWith a new team of authors, \u003ci\u003eLarone’s Medically Important Fungi\u003c\/i\u003e, Seventh Edition, continues the longstanding tradition of high-quality content to expand your knowledge and support your work in clinical mycology by: \u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eProviding detailed descriptions of the major mycoses as viewed in patients’ specimens by direct microscopic examination of stained slides\u003c\/li\u003e\n\u003cli\u003eOffering a logical step-by-step process for identification of cultured organisms, utilizing detailed descriptions, images, pointers on organisms’ similarities and distinctions, and selected references for further information\u003c\/li\u003e\n\u003cli\u003eCovering more than 150 of the fungi most commonly encountered in the clinical mycology laboratory, including new entries for \u003ci\u003eEmergomyces, Metarhizium anisopliae\u003c\/i\u003e, \u003ci\u003eRasamsonia argillacea, Rhinocladiella mackenziei, Schizophyllum commune, \u003c\/i\u003eand \u003ci\u003eThermothelomyces thermophilus\u003c\/i\u003e\n\u003c\/li\u003e\n\u003cli\u003ePresenting details on each organism’s pathogenicity, growth characteristics, relevant biochemical reactions, and microscopic morphology, illustrated with photomicrographs, unique and elegant drawings, and color photos of colony morphology and various test results\u003c\/li\u003e\n\u003cli\u003eExplaining changes in fungal taxonomy and nomenclature that are due to information acquired through molecular taxonomic studies of evolutionary fungal relationships\u003c\/li\u003e\n\u003cli\u003eProviding basic information on molecular diagnostic methods, e.g., nucleic acid amplification and sequencing, MALDI-TOF mass spectrometry, and other commercial platforms\u003c\/li\u003e\n\u003cli\u003eIncluding an extensive section of easy-to-follow lab protocols, a comprehensive list of media and stain procedures, guidance on collection and preparation of patient specimens, and an illustrated glossary\u003c\/li\u003e\n\u003c\/ul\u003e \u003cp\u003eWith \u003ci\u003eLarone’s Medically Important Fungi: A Guide to Identification\u003c\/i\u003e, both novices and experienced professionals in clinical microbiology laboratories can confidently identify commonly encountered fungi.\u003c\/p\u003e","brand":"ASM Press","offers":[{"title":"Default Title","offer_id":47989510242533,"sku":"NP9781683674405","price":135.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9781683674405.jpg?v=1761784395","url":"https:\/\/k12savings.com\/es\/products\/larones-medically-important-fungi-isbn-9781683674405","provider":"K12savings","version":"1.0","type":"link"}