Industrial Proteomics

This book covers both basic elements and the state-of-the-art in applications of proteomics. The first section gives an introduction to the field, from differential approaches to functional proteomics, including yeast two-hybrid, mass spectrometry, mapping of post-translational modifications, and NMR spectroscopy. The second section offers a complete picture of industry studies in proteomics for human health and biotechnology applications. The final section addresses the future, looking at the most promising areas such as protein arrays and the integration of genomic and proteomic tools. Preface.

Contributors.

1. Proteomics: The Basic Overview (Daniel Figeys).

2. Mapping Protein-Protein Interactions (Daniel Figeys).

3. Protein Post-Translational Modifications: Phosphorylation Site Analysis Using Mass Spectrometry (Roland S. Annan and Francesca Zappacosta).

4. The Use of High Throughput Crystallography and In Silico Methods for Structure Based Drug Design (Leslie W. Tarr, Andy J. Jennings and Duncan E. McRee).

5. High Throughput Analysis of Protein Structure by H/D Exchange (H/D-Ex) Mass Spectrometry (Yoshitomo Hamuro, Patricia C. Weber and Patrick R. Griffin).

6. Proteomics Technologies for Identification and Validation of Protein Targets (John E. Hale, Weija Ou, Pavel Shiyanov, Michael D. Knierman and James R. Ludwig).

7. Proteomics Discovery of Biomarkers (Robert Massé and Bernard F. Gibbs).

8. Industrial-Scale Proteomics Analysis of Human Plasma (Keith Rose).

9. Chemical Genomics: Targets on Display (Steve Doberstein, Philip W. Hammond and René S. Hubert).

10. Bioinformatics for Proteomics (Christian Ahrens, Hans Jespersen and Soeren Schandorff).

11. Protein Arrays (David S. Wilson and Steffen Nock).

Index.

"...very well written and cover[s] a wide range of methods." (The Quarterly Review of Biology, September 2005)

"...an excellent text that provides an overview of a number of industrial applications...is useful to anyone involved with proteomics based research." (Clinical Biochemistry, August 2005)

DANIEL FIGEYS, Ph.D., is the Director of the Ottowa Institute of Systems Biology. Prior to joining the institute, Dr. Figeys was senior vice president, systems biology at MDS Proteomics and a research officer at the NRC-Canada. He did his postdoc at the Department of Molecular Biotechnology in Seattle with Dr. R. Aebersold. Industrial Proteomics addresses many of the key challenges faced by researchers in the field of proteomics. It enables them to better understand cellular function and apply their knowledge to make new advances in many fields, including drug discovery, agriculture, and health sciences. Written by a team of leading researchers in biotechnology and pharmaceutical sciences, the book also provides expert guidance for understanding, using, and interpreting the findings of such sophisticated tools as lab-on-a-chip and protein arrays.

The first section of Industrial Proteomics introduces the field, beginning with a discussion of functional proteomics, including yeast two-hybrid assay, mass spectrometry, mapping of post-translational modifications, and nuclear magnetic resonance spectroscopy. Next, structural proteomics is presented with detailed descriptions of X-ray crystallography, insilico prediction, and protein bioinformatics methods. The second section dedicates itself to current biotechnology and pharmaceutical research, replete with examples of actual research that demonstrate the most advanced applications. Topics in this section cover proteomic-based target discovery, biomarker discovery, late-stage drug discovery, crop improvement, and animal studies. The final section provides an expert forecast of the field's promising future, delving into areas such as protein arrays and the integration of genomic and proteomic tools.

Whether you are a novice to the field looking for a solid introduction or an experienced researcher needing to develop your skills and knowledge to make further research advances, Industrial Proteomics is an essential reference. It provides a solid foundation, introduces today's exciting proteomics research, and points to the most promising areas for development.