{"product_id":"immunohistochemistry-and-immunocytochemistry-isbn-9781118717776","title":"Immunohistochemistry and Immunocytochemistry","description":"Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount.\u003cbr\u003e\u003cbr\u003eIt is with this fundamental aim in mind that we have compiled Immunohistochemistry: Essential Methods. We have achieved this by examining each aspect of immunochemistry in turn, with each chapter including detailed information regarding the subject matter in question. Each chapter is written by an expert in their field and includes protocols that are typically used in their own research. Subjects covered are, amongst others, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy. In addition, benefits and limitations of each approach are discussed within the chapters. List of Contributors vii \u003cp\u003ePreface ix\u003c\/p\u003e \u003cp\u003eAcknowledgements xi\u003c\/p\u003e \u003cp\u003e\u003cb\u003eChapter 1: Antibodies for Immunochemistry 1\u003c\/b\u003e\u003cbr\u003e\u003ci\u003eMark Cooper and Sheriden Lummas\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 1\u003c\/p\u003e \u003cp\u003eImmunogens for Antibody Production 5\u003c\/p\u003e \u003cp\u003eAntibody Production 12\u003c\/p\u003e \u003cp\u003eAntibody Purification 16\u003c\/p\u003e \u003cp\u003eFragment Antibody Preparations 20\u003c\/p\u003e \u003cp\u003eAntibody Labelling 21\u003c\/p\u003e \u003cp\u003eAntibody Stability and Storage 23\u003c\/p\u003e \u003cp\u003eReferences 24\u003c\/p\u003e \u003cp\u003e\u003cb\u003eChapter 2: The Selection of Reporter Labels 25\u003c\/b\u003e\u003cbr\u003e\u003ci\u003eJudith Langenick\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 25\u003c\/p\u003e \u003cp\u003eEnzymatic Labels 26\u003c\/p\u003e \u003cp\u003eFluorescence Detection 29\u003c\/p\u003e \u003cp\u003eReferences 32\u003c\/p\u003e \u003cp\u003e\u003cb\u003eChapter 3: Immunohistochemistry and Immunocytochemistry 35\u003c\/b\u003e\u003cbr\u003e\u003ci\u003eSimon Renshaw\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eSpecimen Formats for Immunochemistry 36\u003c\/p\u003e \u003cp\u003eFixation 37\u003c\/p\u003e \u003cp\u003eProcessing Tissue Blocks to Paraffin Wax 46\u003c\/p\u003e \u003cp\u003eMicrotomy 47\u003c\/p\u003e \u003cp\u003eTissue Microarrays 47\u003c\/p\u003e \u003cp\u003eSpecimen Storage 48\u003c\/p\u003e \u003cp\u003eDecalcification 49\u003c\/p\u003e \u003cp\u003eAntigen Retrieval 50\u003c\/p\u003e \u003cp\u003eControls 56\u003c\/p\u003e \u003cp\u003eImmunochemical Staining Techniques (Optimizing a New Antibody) 57\u003c\/p\u003e \u003cp\u003eCounterstains 71\u003c\/p\u003e \u003cp\u003eMounting 74\u003c\/p\u003e \u003cp\u003eTroubleshooting 76\u003c\/p\u003e \u003cp\u003eExamples of Immunostaining Photomicrographs 76\u003c\/p\u003e \u003cp\u003eAcknowledgements 101\u003c\/p\u003e \u003cp\u003eReferences 101\u003c\/p\u003e \u003cp\u003e\u003cb\u003eChapter 4: Multiple Immunochemical Staining Techniques 103\u003c\/b\u003e\u003cbr\u003e\u003ci\u003eSofia Koch\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 103\u003c\/p\u003e \u003cp\u003eMethods and Approaches 115\u003c\/p\u003e \u003cp\u003eReferences 122\u003c\/p\u003e \u003cp\u003e\u003cb\u003eChapter 5: Quality Assurance in Immunochemistry 123\u003c\/b\u003e\u003cbr\u003e\u003ci\u003ePeter Jackson and Michael Gandy\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 123\u003c\/p\u003e \u003cp\u003eMethods and Approaches 125\u003c\/p\u003e \u003cp\u003eAutomated Immunochemical Staining 147\u003c\/p\u003e \u003cp\u003eTroubleshooting 149\u003c\/p\u003e \u003cp\u003eReferences 154\u003c\/p\u003e \u003cp\u003e\u003cb\u003eChapter 6: Automated Immunochemistry 157\u003c\/b\u003e\u003cbr\u003e\u003ci\u003eEmanuel Schenck and Simon Renshaw\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 157\u003c\/p\u003e \u003cp\u003eMethods and Approaches 160\u003c\/p\u003e \u003cp\u003eOther Forms of Automation 164\u003c\/p\u003e \u003cp\u003eReferences 168\u003c\/p\u003e \u003cp\u003e\u003cb\u003eChapter 7: Confocal Microscopy 169\u003c\/b\u003e\u003cbr\u003e\u003ci\u003eAnn Wheeler\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 169\u003c\/p\u003e \u003cp\u003eWhen Should Confocal be Used? 173\u003c\/p\u003e \u003cp\u003eApplications: For Example Co-localization, Quantification, 3D Visualization and Kinetics 173\u003c\/p\u003e \u003cp\u003eHow To Set Up a Confocal Experiment? 174\u003c\/p\u003e \u003cp\u003eReferences 198\u003c\/p\u003e \u003cp\u003eFurther Readings 198\u003c\/p\u003e \u003cp\u003e\u003cb\u003eChapter 8: Ultrastructural Immunochemistry 199\u003c\/b\u003e\u003cbr\u003e\u003ci\u003eJeremy Skepper and Janet Powell\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eIntroduction 199\u003c\/p\u003e \u003cp\u003eMethods and Approaches 207\u003c\/p\u003e \u003cp\u003eReferences 222\u003c\/p\u003e \u003cp\u003eIndex 227\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSimon Renshaw\u003c\/b\u003e is the editor of \u003ci\u003eImmunohistochemistry and Immunocytochemistry: Essential Methods\u003c\/i\u003e, 2nd Edition, published by Wiley.\u003c\/p\u003e \u003cp\u003eImmunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount.\u003c\/p\u003e \u003cp\u003e\u003ci\u003eImmunohistochemistry and Immunocytochemistry: Essential Methods\u003c\/i\u003e is the second edition of \u003ci\u003eImmunohistochemisrty: Methods Express\u003c\/i\u003e, previously published by Scion Publishing. The book is a comprehensive research guide that describes the methodologies of immunochemistry. Each chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good theoretical information and practical advice, for immediate use at the bench. Subjects covered include, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy.\u003c\/p\u003e \u003cp\u003eThis book offers a wealth of knowledge to the novice immunochemist, who wishes to understand fully the theory and practice of immunochemical staining techniques and obtain reliable and reproducible data time and time again. For the experienced immunochemist, it is a comprehensive reference guide, allowing optimization of existing immunochemical staining protocols and a resource to further increase knowledge and understanding.\u003c\/p\u003e","brand":"Wiley-Blackwell","offers":[{"title":"Default Title","offer_id":47989403386085,"sku":"NP9781118717776","price":142.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9781118717776.jpg?v=1761783974","url":"https:\/\/k12savings.com\/es\/products\/immunohistochemistry-and-immunocytochemistry-isbn-9781118717776","provider":"K12savings","version":"1.0","type":"link"}