{"product_id":"human-stem-cell-technology-and-biology-isbn-9780470595459","title":"Human Stem Cell Technology and Biology","description":"\u003ci\u003eHuman Stem Cell Technology \u0026amp; Biology: A Research Guide and Laboratory Manual\u003c\/i\u003e integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells.  \u003cp\u003eKey Features:\u003c\/p\u003e \u003cul\u003e \u003cli\u003eAn authoritative, comprehensive, multimedia training manual for stem cell researchers\u003c\/li\u003e \u003cli\u003eEasy to follow step-by-step laboratory protocols and instructional videos provide a valuable resource\u003c\/li\u003e \u003cli\u003eA must-have for developing laboratory course curriculums, training courses, and workshops in stem cell biology\u003c\/li\u003e \u003cli\u003ePerspectives written by the world leaders in the field\u003c\/li\u003e \u003cli\u003eIntroductory chapters will provide background information\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThe volume will be a valuable reference resource for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field.\u003c\/p\u003e  Foreword.  \u003cp\u003ePreface.\u003c\/p\u003e \u003cp\u003eAcknowledgments.\u003c\/p\u003e \u003cp\u003eEditors and Contributors.\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSECTION I: INTRODUCTION.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1 Introduction to Pluripotent Stem Cells: Biology and Applications (\u003ci\u003eMaria Borowski and Gary S. Stein\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e2 Researching and Obtaining Established Stem Cell Lines (\u003ci\u003eMai X. Luong, Kelly P. Smith, and Gary S. Stein\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSECTION II: LABORATORY GUIDE FOR HUMAN STEM CELL CULTURE: PLURIPOTENT STEM CELL CULTURE.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3 Basics of Cell Culture (\u003ci\u003eAlicia Allaire, Mai X. Luong, and Kelly P. Smith\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e4 The Stem Cell Laboratory (\u003ci\u003eAlicia Allaire, Mai X. Luong, and Kelly P. Smith\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e5 Reagent Preparation (\u003ci\u003eAlicia Allaire, Mai X. Luong, and Kelly P. Smith\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e6 Preparation of Mouse Embryonic Fibroblasts for Culture of Human Embryonic Stem Cells (\u003ci\u003eMeng-Jiao Shi, Maria Borowski, and Kimberly Stencel\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e7 Thawing and Seeding of Frozen Mouse Embryonic Fibroblasts (\u003ci\u003eMeng-Jiao Shi, Maria Borowski, and Kimberly Stencel\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e8 Thawing, Seeding, and Changing the Medium of Human Embryonic Stem Cells (\u003ci\u003eMeng-Jiao Shi, Kimberly Stencel, and Maria Borowski\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e9 Passaging of Human Embryonic Stem Cells on Inactivated Mouse Embryonic Fibroblast Plates (\u003ci\u003eMeng-Jiao Shi, Kimberly Stencel, and Maria Borowski\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e10 Harvesting Human Embryonic Stem Cells for Cryopreservation (\u003ci\u003eMeng-Jiao Shi, Kimberly Stencel, and Maria Borowski\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e11 Human Embryonic Stem Cell Culture on BD Matrigel™with mTeSR®1 Medium (\u003ci\u003eMeng-Jiao Shi, Kimberly Stencel, and Maria Borowski\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSECTION III: LABORATORY GUIDE FOR HUMAN STEM CELL CULTURE: CHARACTERIZATION OF PLURIPOTENT STEM CELLS.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e12 Defining Pluripotency (\u003ci\u003eKelly P. Smith and Mai X. Luong\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e13 Characterization of Human Embryonic Stem Cells by Immunofluorescence Microscopy (\u003ci\u003eShirwin M. Pockwinse and Prachi N. Ghule\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e14 Preparation of Human Embryonic Stem Cell Samples for Flow Cytometry (\u003ci\u003eMeng-Jiao Shi, Kimberly Stencel, and Maria Borowski\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e15 Characterization of Human Embryonic Stem Cell Gene Expression Using Reverse Transcription Polymerase Chain Reaction (\u003ci\u003eAlicia Allaire, Kelly P. Smith, and Mai X. Luong\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e16 Determining Pluripotency of Human Embryonic Stem Cells: Embryoid Body Formation (\u003ci\u003eShirwin M. Pockwinse and Prachi N. Ghule\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e17 Characterization of Human Embryonic Stem Cells by Cytogenetics: Karyotyping and Fluorescence \u003ci\u003eIn Situ\u003c\/i\u003e Hybridization (\u003ci\u003eShirwin M. Pockwinse, Prachi N. Ghule and Anne Higgins\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e18 High-Resolution Chromatin Immunoprecipitation Assay (\u003ci\u003eBeatriz Perez-Cadahıa, Bojan Drobic, and James R. Davie\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e19 Assaying Pluripotency Via Teratoma Formation (\u003ci\u003eM. William Lensch and Tan A. Ince\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSECTION\u003c\/b\u003e \u003cb\u003eIV:\u003c\/b\u003e \u003cb\u003ePERSPECTIVES IN HUMAN STEM CELL TECHNOLOGIES.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e20 Genomic Analysis of Pluripotent Stem Cells (\u003ci\u003eDavid Lapointe\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e21 Proteomic Analysis of Human Pluripotent Cells (\u003ci\u003eAndy T. Y. Lau, Yan-Ming Xu, and Jen-Fu Chiu\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e22 Bioinformatics Strategies for Understanding Gene Expression in Human Pluripotent Cells (\u003ci\u003eGustavo Glusman, Bruz Marzolf, Kai Wang, Ji-Hoon Cho, Burak Kutlu, and Qiang Tian\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e23 Epigenetic Analysis of Pluripotent Cells (\u003ci\u003eMojgan Rastegar, Genevieve P. Delcuve, and James R. Davie\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e24 Differentiation of Pluripotent Stem Cells: An Overview (\u003ci\u003eJeremy Micah Crook\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e25 Cellular Reprogramming: Current Technology, Perspectives, and Generation of Induced Pluripotent Cells (\u003ci\u003eTanja Dominko\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSECTION V: APPLICATIONS OF HUMAN EMBRYONIC STEM CELLS.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e26 Human Pluripotent Cells: The Biology of Pluripotency (\u003ci\u003eLi-Fang Chu and Thomas P. Zwaka\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e27 Human Pluripotent Cells for Regenerative Medicine: Potential Applications for Regenerative Medicine (\u003ci\u003eChristopher C. Ford and Darrell N. Kotton\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e28 Therapeutic Applications of Human Embryonic Stem Cells (\u003ci\u003eShi-Jiang Lu, Irina Klimanskaya, Edmund Mickunas, and Robert Lanza\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e29 Human Stem Cells for Drug Screening, Toxicity, Specificity, and Off-Target Effects (\u003ci\u003eArnaud Lacoste and Mark Burcin\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e30 Interfacing Stem Cells with Gene Therapy (\u003ci\u003eChristian Mueller and Terence R. Flotte\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e31 Tissue Engineering for Stem Cell Mediated Regenerative Medicine (\u003ci\u003eJanet Zoldan, Thomas P. Kraehenbuehl, Abigail K. R. Lytton-Jean, Robert S. Langer, and Daniel G. Anderson\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e\u003cb\u003eGlossary.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eIndex.\u003c\/b\u003e\u003c\/p\u003e  \"The topic is intrinsically fascinating and clinically relevant ... I recommend this book for novice and experienced scientists interested in stem cell biology. It will serve as an excellent reference manual and practical laboratory workbook.\" (Doody's, 17 February 2012)  \u003cp\u003e\"We would recommend this readable and well-organised book to all laboratories dealing with human ES cells ... This book will act as an excellent guide.\" (Development Journal, 1 December 2011)\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003eGary Stein\u003c\/b\u003e, lead Editor for this publication, is currently Professor \u0026amp; Chairman of the Deartment of Cell Biology at the University of Massachusetts Medical School (Worcester, MA); Deputy Director of the UMASS Memorial Cancer Center; Appointed Haidak Distinguished Prof. \u0026amp; Chair of Cell Biology; Interim Director of the Center for Stem Cell Biology and Regenerative Medicine; and Interim Director of the UMASS Cancer Center. He is Executive Editor of \u003ci\u003eJournal of Cellular Biochemistry\u003c\/i\u003e, \u003ci\u003eJournal of Cellular Physiology\u003c\/i\u003e, \u003ci\u003eCritical Reviews in Eukaryotic Gene expression\u003c\/i\u003e, and \u003ci\u003eMolecular Biology Reports\u003c\/i\u003e, and has served on numerous Editorial Boards. He has published over 700 full length research articles, and has authored numerous books.\u003c\/p\u003e \u003cb\u003eThe first comprehensive multimedia training manual for stem cell education and research\u003c\/b\u003e \u003cp\u003eThe first resource of its kind, \u003ci\u003eHuman Stem Cell Technology and Biology\u003c\/i\u003e presents laboratory techniques and protocols that have proven to be effective for the culture of pluripotent stem cells, as well as current perspectives in the field. Edited by a team of professionals at the forefront of discovery and innovation in stem cell research, education, and development of infrastructure, this authoritative multimedia training manual distinguishes itself by integrating readily accessible text, electronic, and video components to explain the biology, technology, and applications of human embryonic stem cells.\u003c\/p\u003e \u003cp\u003eThe first section of the text begins with an overview of the research that established the foundation for current initiatives in stem cell research; it goes on to describe the best methods for researching and obtaining pluripotent cell lines. The second section provides the skills and techniques necessary for the culture, maintenance, and characterization of pluripotent stem cells. This section has been developed as a multimedia stem cell course in order to present the most comprehensive instruction possible. The third section presents an in-depth perspective on the current status of the stem cell research field, and the fourth section explores applications, describing the most recent advances in research and the interface with gene therapy, tissue engineering, and drug testing.\u003c\/p\u003e \u003cp\u003e\u003cb\u003eFeatures of this one-of-a-kind reference include:\u003c\/b\u003e\u003c\/p\u003e \u003cul\u003e \u003cli\u003e \u003cp\u003eIntroductory chapters that provide background information to scientists entering the field\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eState-of-the-art, contemporary perspectives written by international leaders in the field\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003ePrintable laboratory protocols in PDF form, accessible on the book's Web site\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eOnline updates of the rapidly developing strategies and research protocols that support stem cell research\u003c\/p\u003e \u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003ci\u003eHuman Stem Cell Technology and Biology\u003c\/i\u003e is an invaluable reference and laboratory manual for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field. Whether it is used to design a laboratory curriculum, workshop, or training course, or simply to assist in performing research in stem cell biology, this volume is a must-have resource of protocols, information, and perspectives for professional laboratories, colleges, universities, and vocational schools.\u003c\/p\u003e","brand":"Wiley-Blackwell","offers":[{"title":"Default Title","offer_id":47989391065317,"sku":"NP9780470595459","price":190.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9780470595459.jpg?v=1761783926","url":"https:\/\/k12savings.com\/es\/products\/human-stem-cell-technology-and-biology-isbn-9780470595459","provider":"K12savings","version":"1.0","type":"link"}