{"product_id":"guide-to-clinical-and-diagnostic-virology-isbn-9781555819910","title":"Guide to Clinical and Diagnostic Virology","description":"\u003cp\u003eThe explosion in clinical testing has been especially rapid in virology, where emerging viruses and growing numbers of viral infections are driving advances. The \u003ci\u003eGuide to Clinical and Diagnostic Virology\u003c\/i\u003e offers a digestible view of the breadth and depth of information related to clinical virology, providing a practical, working knowledge of the wide array of viruses that cause human disease.\u003c\/p\u003e \u003cp\u003eIntroductory chapters cover the basics of clinical virology and laboratory diagnosis of infections, including virus structure, life cycle, transmission, taxonomy, specimen types and handling, and a comparison of assays used for detection. Detailed sections on important topics include\u003c\/p\u003e \u003cul\u003e \u003cli\u003eViral pathogens and their clinical presentations\u003c\/li\u003e \u003cli\u003eDiagnostic assays and techniques, including culture-based, immunological, and molecular\u003c\/li\u003e \u003cli\u003ePrevention and management of viral infections, with guidance on biosafety, vaccines, and antiviral therapies\u003c\/li\u003e \u003cli\u003eThe regulatory environment for laboratory testing, including regulatory requirements and assay performance and interpretation\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eCritical concepts are carefully curated and concisely summarized and presented with detailed illustrations that aid comprehension, along with important highlights and helpful hints. These features, plus question sections that reinforce significant ideas and key concepts, make this an invaluable text for anyone looking for an accessible route through clinical and diagnostic virology. Laboratory technologists, medical students, infectious disease and microbiology fellows, pathology residents, researchers, and everyone involved with viruses in the clinical setting will find the Guide to Clinical and Diagnostic Virology an excellent text as well as companion to clinical virology references.\u003c\/p\u003e \u003cp\u003ePreface xi\u003c\/p\u003e \u003cp\u003eAcknowledgments xii\u003c\/p\u003e \u003cp\u003eAbbreviations xiii\u003c\/p\u003e \u003cp\u003eAbout the Author xvi\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSECTION I: Foundations of Clinical Virology 3\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Introduction to Viruses 5\u003cbr\u003e\u003c\/b\u003eVirus structure, life cycle, Baltimore classification, transmission, nomenclature\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Laboratory Diagnosis of Viral Infections 17\u003cbr\u003e\u003c\/b\u003eDifferential diagnosis for viral syndromes, specimen collection, general comparison of diagnostic techniques\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSECTION II: Viral Pathogens and Clinical Presentation 23\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Respiratory Viruses 25\u003cbr\u003e\u003c\/b\u003eInfluenza virus, respiratory syncytial virus, parainfluenza virus, human metapneumovirus, rhinovirus, coronaviruses, mumps virus\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Viruses with Dermatologic Manifestations 43\u003cbr\u003e\u003c\/b\u003eHerpes simplex virus 1 and 2, varicella-zoster virus, measles virus, rubella virus, human herpesviruses 6 and 7, molluscum contagiosum virus, smallpox virus, comparison of herpesviruses 1 to 8\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Gastrointestinal and Fecal-Oral Hepatitis Viruses 61\u003cbr\u003e\u003c\/b\u003eRotavirus, norovirus, astrovirus, sapovirus, hepatitis A virus, hepatitis E virus\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Viruses That Can Cause Multiple Syndromes 71\u003cbr\u003e\u003c\/b\u003eEnteroviruses and parechoviruses, adenoviruses, parvovirus B19, human bocavirus\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Opportunistic Viruses Associated with Immunosuppression 83\u003cbr\u003e\u003c\/b\u003eCytomegalovirus, BK virus, JC virus\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Blood-Borne Hepatitis Viruses 91\u003cbr\u003e\u003c\/b\u003eHepatitis B, C, and D viruses, and comparison of hepatitis viruses A through E\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Human Retroviruses 105\u003cbr\u003e\u003c\/b\u003eHuman immunodeficiency virus, human T-cell lymphotropic virus\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Oncogenic Viruses 121\u003cbr\u003e\u003c\/b\u003eHuman papillomavirus, Epstein-Barr virus, human herpesvirus 8\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Zoonotic Viruses 133\u003cbr\u003e\u003c\/b\u003eRabies virus, Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, hantaviruses, lymphocytic choriomeningitis virus, monkeypox virus, herpes B virus, Hendra and Nipah viruses, comparison of zoonotic viruses\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Arboviruses 147\u003cbr\u003e\u003c\/b\u003eMosquitoes, ticks, dengue virus, yellow fever virus, chikungunya virus, West Nile virus, Zika virus, eastern, western, and Venezuelan equine encephalitis viruses, Japanese encephalitis virus, Powassan virus, tick-borne encephalitis virus, Rift Valley fever virus, comparison of arboviruses\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSECTION III: Diagnostic Assays and Techniques 161\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Culture and Tissue-Based Diagnostic Techniques 163\u003cbr\u003e\u003c\/b\u003eConventional viral culture and cell lines, cytopathic effect, viral growth rates, shell vial assays, hemadsorption, quantification by plaque-forming units and TCID50, histopathology and cytopathology of viruses, in situ hybridization\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 Diagnostic Techniques Based on Immunological Interactions 175\u003cbr\u003e\u003c\/b\u003eEnzyme immunoassays including ELISAs, chemiluminescent immunoassays, and immunoblot assays, immunofluorescence assays, immunochromatographic (lateral flow) assays, hemagglutination inhibition and plaque reduction neutralization assays, serologic assays, kinetics and interpretation of antibody responses, comparison of immunoassays\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 Molecular Techniques: Nucleic Acid Amplification 189\u003cbr\u003e\u003c\/b\u003eImportance of nucleic acid structure, sample processing, PCR, reverse transcription-PCR, real-time PCR, quantitative vs. qualitative PCR, melt curve analysis, droplet digital PCR, nested PCR, multiplex PCR, transcription-mediated amplification, PCR controls, minimizing contamination\u003c\/p\u003e \u003cp\u003e\u003cb\u003e16 Molecular Techniques: Sequencing 207\u003cbr\u003e\u003c\/b\u003eApplications of sequencing, first generation (Sanger, dideoxy chain termination) and next generation (Illumina, Ion Torrent, PacBio, and Oxford Nanopore) sequencing, sample preprocessing, library generation, amplification techniques, quality and depth of coverage, data analysis, comparison of all platforms\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSECTION IV: Prevention and Management of Viral Infections 227\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e17 Biosafety 229\u003cbr\u003e\u003c\/b\u003eBiosafety levels, select agents, reportable diseases, personal protective equipment, biosafety cabinets, isolation precautions\u003c\/p\u003e \u003cp\u003e\u003cb\u003e18 Vaccines 237\u003cbr\u003e\u003c\/b\u003eActive and passive immunity, types of vaccines, route of administration, risk and other consequences of vaccination, antibody-dependent enhancement, table of available viral and other vaccines, diagram of routine vaccination schedule\u003c\/p\u003e \u003cp\u003e\u003cb\u003e19 Antivirals 249\u003cbr\u003e\u003c\/b\u003eAntivirals against herpesviruses, human papillomaviruses, influenza virus, respiratory syncytial virus, hepatitis B virus, hepatitis C virus, antiretrovirals and antivirals with broad coverage, immunomodulators, comparison of antivirals, mechanisms of action\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSECTION V: The Regulatory Environment for Laboratory Testing 265\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e20 Regulatory Requirements 267\u003cbr\u003e\u003c\/b\u003eClassification of diagnostic assays, test complexity, role of CMS, CDC, CLSI, CLIA, inspections, proficiency testing, billing and coding\u003c\/p\u003e \u003cp\u003e\u003cb\u003e21 Assay Performance and Interpretation 275\u003cbr\u003e\u003c\/b\u003eValidation\/verification, performance characteristics (precision, accuracy, reportable range, reference range, analytic sensitivity, analytic specificity), diagnostic and clinical sensitivity and specificity, prevalence, predictive value, ROC curves\u003c\/p\u003e \u003cp\u003eReferences 285\u003c\/p\u003e \u003cp\u003eAnswers 289\u003c\/p\u003e \u003cp\u003eIndex 293\u003c\/p\u003e   \u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003eAt A Glance\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003eThe explosion in clinical testing has been especially rapid in virology, where emerging viruses and growing numbers of viral infections are driving advances. The Guide to Clinical and Diagnostic Virology offers a digestible view of the breadth and depth of information related to clinical virology, providing a practical, working knowledge of the wide array of viruses that cause human disease. Introductory chapters cover the basics of clinical virology and laboratory diagnosis of infections, including virus structure, life cycle, transmission, taxonomy, specimen types and handling, and a comparison of assays used for detection.\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003eDescription\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003eThis is an abbreviated overview of the field of virology, starting with classification and nomenclature and basic laboratory diagnostic techniques, and finishing with chapters on prevention, management, and regulatory issues. It is a review, complete with tables, illustrations, and end-of-chapter quizzes to improve comprehension.\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003ePurpose\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003eIn the preface, the author acknowledges that the field of clinical and diagnostic virology is rapidly evolving and declares the purpose is to provide a \"simple reference for medical and scientific professionals.\" She achieves this by identifying key concepts in the field of virology and dividing them into chapters organized in an outline format. Combined with simple but effective illustrations and tables, this book is a worthy introduction to a field that can otherwise overwhelm students.\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003eAudience\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003eThe author specifies that this book is designed for a wide range of professionals in medical and laboratory fields. It certainly serves as a well-organized introduction to an otherwise complex field and it can be used as a review or an outline for more in-depth study of the topics. Graduate level students and fellows in microbiology and infectious diseases will need to supplement with books that cover pathophysiology, mechanisms of action, and diagnostic techniques in greater depth. The author has her PhD in virology and gene therapy from Mayo Clinic with a research focus on viruses as vectors and oncolytic agents. She is extensively published and has a clinical microbiology fellowship from Mayo Clinic.\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003eFeatures\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003eThis book does an excellent job of highlighting concepts that are essential to understand in clinical and diagnostic virology. With well-organized chapters, it breaks down an otherwise complicated field into outlines, making the book easy to read. Key concepts are highlighted in the margins and important terminology is in bold, further enhancing readability. Illustrations and tables are simple, yet well organized and effective. Sections and chapters are demarcated by colors for effective studying, and the multiple-choice questions at the end of each chapter are well-written and designed for maximum comprehension. The references are not terribly complete and further study of topics would be best covered by a comprehensive virology textbook, but this book very effectively meets its objectives.\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003eAssessment\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003eEither as an introduction to the field of virology for college students or as a review for students in the field of microbiology, infectious diseases, or pathology, this is a well-organized and engaging book. The author carefully and appropriately chose key concepts and achieved an excellent balance of depth and breadth on each topic — very difficult to do for a review of such a comprehensive subject as virology. I enjoyed reading this book and would recommend it to my internal medicine residents and infectious diseases fellows.\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003eReview Questionnaire\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003eRange Question Score\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003e1-10 Are the author's objectives met? 10\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003e1-10 Rate the worthiness of those objectives. 10\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003e1-5 Is this written at an appropriate level? 5\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003e1-5 Is there significant duplication? (1=significant, 5=insignificant) 4\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003e1-5 Are there significant omissions? (1=significant, 5=insignificant) 4\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003e1-5 Rate the authority of the authors. 4\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003e1-5 Are there sufficient illustrations? 5\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003e1-5 Rate the pedagogic value of the illustrations. 4\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003e1-5 Rate the print quality of the illustrations. 4\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003e1-5 Are there sufficient references? 5\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003e1-5 Rate the currency of the references. 4\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003e1-5 Rate the pertinence of the references. 5\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003e1-5 Rate the helpfulness of the index. 5\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003e1-5 If important in this specialty, rate the physical appearance of the book 5\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003e1-10 Is this a worthwhile contribution to the field? 8\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003e1-10 If this is a 2nd or later edition, is this new edition needed? N\/A\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan lang=\"EN-CA\"\u003e-Cynthia Rivera, MD, FACP\u003c\/span\u003e\u003c\/b\u003e\u003cspan lang=\"EN-CA\"\u003e (Florida International University Health)\u003c\/span\u003e  \u003c\/p\u003e\u003cp class=\"MsoNormal\"\u003e\u003cspan lang=\"EN-CA\"\u003e \u003cp\u003e\u003cb\u003eReeti Khare\u003c\/b\u003e, PhD, D(ABMM), is the Director of Microbiology at Northwell Health Laboratories in New York. She received her PhD in Virology and Gene Therapy at Mayo Clinic and did a postdoctoral fellowship at the University of Washington. Her research involved reengineering viral vectors, developing adenoviruses for liver gene therapy, and creating viral vector vaccines against MRSA. She returned to Mayo Clinic for her clinical microbiology fellowship and is a diplomate of the American Board of Medical Microbiology. Reeti enjoys teaching and learning about microbiology and has authored numerous publications, chapters, and reviews. At Northwell Health Labs she continues to pursue clinical research and provide student education, and is responsible for laboratory oversight, improving efficiency, designing workflows, and diagnostic microbiology testing.\u003c\/p\u003e  \u003cp\u003e\u003cb\u003eGuide to Clinical and Diagnostic Virology\u003c\/b\u003e \u003c\/p\u003e\n\u003cp\u003eFirst Edition  \u003c\/p\u003e\n\u003cp\u003eThe explosion in clinical testing has been especially rapid in virology, where emerging viruses and growing numbers of viral infections are driving advances. The Guide to Clinical and Diagnostic Virology offers a digestible view of the breadth and depth of information related to clinical virology, providing a practical, working knowledge of the wide array of viruses that cause human disease.   \u003c\/p\u003e\n\u003cp\u003eIntroductory chapters cover the basics of clinical virology and laboratory diagnosis of infections, including virus structure, life cycle, transmission, taxonomy, specimen types and handling, and a comparison of assays used for detection. Detailed sections on important topics include \u003c\/p\u003e\n\u003cul\u003e \u003cli\u003eViral pathogens and their clinical presentations\u003c\/li\u003e \u003cli\u003eDiagnostic assays and techniques, including culture-based, immunological, and molecular\u003c\/li\u003e \u003cli\u003ePrevention and management of viral infections, with guidance on biosafety, vaccines, and antiviral therapies\u003c\/li\u003e \u003cli\u003eThe regulatory environment for laboratory testing, including regulatory requirements and assay performance and interpretation\u003c\/li\u003e \u003c\/ul\u003e  \u003cp\u003eCritical concepts are carefully curated and concisely summarized and presented with detailed illustrations that aid comprehension, along with important highlights and helpful hints. These features, plus question sections that reinforce significant ideas and key concepts, make this an invaluable text for anyone looking for an accessible route through clinical and diagnostic virology. Laboratory technologists, medical students, infectious disease and microbiology fellows, pathology residents, researchers, and everyone involved with viruses in the clinical setting will find the Guide to Clinical and Diagnostic Virology an excellent text as well as companion to clinical virology references.\u003c\/p\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"ASM Press","offers":[{"title":"Default Title","offer_id":47989317730533,"sku":"NP9781555819910","price":123.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9781555819910.jpg?v=1761783646","url":"https:\/\/k12savings.com\/es\/products\/guide-to-clinical-and-diagnostic-virology-isbn-9781555819910","provider":"K12savings","version":"1.0","type":"link"}