{"product_id":"gene-cloning-and-dna-analysis-isbn-9781119640783","title":"Gene Cloning and DNA Analysis","description":"\u003cp\u003eKnown worldwide as the standard introductory text to this important and exciting area of study, \u003ci\u003eGene Cloning and DNA Analysis: An Introduction,\u003c\/i\u003e 8th Edition preserves the tradition of excellence created by previous editions. Comprehensive and authoritative, the book explores all of the topics crucial to an understanding of gene cloning in an approachable way. An easy-to-follow and user-friendly layout is presented in full-color throughout the volume, making it simple to absorb the clear and accessible material contained within.\u003c\/p\u003e \u003cp\u003e\u003ci\u003eGene Cloning and DNA Analysis: An Introduction,\u003c\/i\u003e 8th Edition contains updated and extended coverage of gene editing strategies like CRISPR\/Cas, rewritten chapters on DNA sequencing and genome studies, as well as new material on real-time PCR and typing of human disease mutations. Over 250 full-color illustrations are included to bring to life the comprehensive content. The book also covers topics like:\u003c\/p\u003e \u003cul\u003e \u003cli\u003eThe strategies used by researchers and industry practitioners to assemble genome sequences\u003c\/li\u003e \u003cli\u003eNext generation sequencing methods and descriptions of their applications in studying genomes and transcriptomes\u003c\/li\u003e \u003cli\u003eIncludes the use and application of gene editing strategies\u003c\/li\u003e \u003cli\u003eInterbreeding between Neanderthals and Homo Sapiens\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003ci\u003eGene Cloning and DNA Analysis: An Introduction,\u003c\/i\u003e 8th Edition is an invaluable introductory text for students in classes like genetics and genomics, molecular biology, biochemistry, immunology, and applied biology. It also belongs on the bookshelves of every professional who desires to improve their understanding of the basics of gene cloning or DNA analysis.\u003c\/p\u003e \u003cp\u003ePreface to the Eighth Edition xv\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart I The Basic Principles of Gene Cloning and DNA Analysis 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1 Why Gene Cloning and DNA Analysis are Important 3\u003c\/p\u003e \u003cp\u003e2 Vectors for Gene Cloning: Plasmids and Bacteriophages 15\u003c\/p\u003e \u003cp\u003e3 Purification of DNA from Living Cells 29\u003c\/p\u003e \u003cp\u003e4 Manipulation of Purified DNA 53\u003c\/p\u003e \u003cp\u003e5 Introduction of DNA into Living Cells 83\u003c\/p\u003e \u003cp\u003e6 Cloning Vectors for \u003ci\u003eE. coli \u003c\/i\u003e101\u003c\/p\u003e \u003cp\u003e7 Cloning Vectors for Eukaryotes 121\u003c\/p\u003e \u003cp\u003e8 How to Obtain a Clone of a Specific Gene 145\u003c\/p\u003e \u003cp\u003e9 The Polymerase Chain Reaction 169\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart II The Applications of Gene Cloning and DNA Analysis in Research 187\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e10 Sequencing Genes and Genomes 189\u003c\/p\u003e \u003cp\u003e11 Studying Gene Expression and Function 217\u003c\/p\u003e \u003cp\u003e12 Studying Genomes 243\u003c\/p\u003e \u003cp\u003e13 Studying Transcriptomes and Proteomes 259\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart III The Applications of Gene Cloning and DNA Analysis in Biotechnology 275\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e14 Production of Protein from Cloned Genes 277\u003c\/p\u003e \u003cp\u003e15 Gene Cloning and DNA Analysis in Medicine 301\u003c\/p\u003e \u003cp\u003e16 Gene Cloning and DNA Analysis in Agriculture 327\u003c\/p\u003e \u003cp\u003e17 Gene Cloning and DNA Analysis in Forensic Science and Archaeology 355\u003c\/p\u003e \u003cp\u003eGlossary 377\u003c\/p\u003e \u003cp\u003eIndex 395\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003ePreface to the Eighth Edition xv\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart I The Basic Principles of Gene Cloning and DNA Analysis 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Why Gene Cloning and DNA Analysis are Important 3\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1.1 The early development of genetics 4\u003c\/p\u003e \u003cp\u003e1.2 The advent of gene cloning and the polymerase chain reaction 4\u003c\/p\u003e \u003cp\u003e1.3 What is gene cloning? 5\u003c\/p\u003e \u003cp\u003e1.4 What is PCR? 5\u003c\/p\u003e \u003cp\u003e1.5 Why gene cloning and PCR are so important 8\u003c\/p\u003e \u003cp\u003e1.5.1 Obtaining a pure sample of a gene by cloning 8\u003c\/p\u003e \u003cp\u003e1.5.2 PCR can also be used to purify a gene 10\u003c\/p\u003e \u003cp\u003e1.6 How to find your way through this book 11\u003c\/p\u003e \u003cp\u003eFurther reading 13\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Vectors for Gene Cloning: Plasmids and Bacteriophages 15\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e2.1 Plasmids 15\u003c\/p\u003e \u003cp\u003e2.1.1 Size and copy number 17\u003c\/p\u003e \u003cp\u003e2.1.2 Conjugation and compatibility 18\u003c\/p\u003e \u003cp\u003e2.1.3 Plasmid classification 19\u003c\/p\u003e \u003cp\u003e2.1.4 Plasmids in organisms other than bacteria 19\u003c\/p\u003e \u003cp\u003e2.2 Bacteriophages 19\u003c\/p\u003e \u003cp\u003e2.2.1 The phage infection cycle 20\u003c\/p\u003e \u003cp\u003e2.2.2 Lysogenic phages 20\u003c\/p\u003e \u003cp\u003e2.2.3 Viruses as cloning vectors for other organisms 26\u003c\/p\u003e \u003cp\u003eFurther reading 27\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Purification of DNA from Living Cells 29\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e3.1 Preparation of total cell DNA 30\u003c\/p\u003e \u003cp\u003e3.1.1 Growing and harvesting a bacterial culture 30\u003c\/p\u003e \u003cp\u003e3.1.2 Preparation of a cell extract 31\u003c\/p\u003e \u003cp\u003e3.1.3 Purification of DNA from a cell extract 33\u003c\/p\u003e \u003cp\u003e3.1.4 Concentration of DNA samples 37\u003c\/p\u003e \u003cp\u003e3.1.5 Measurement of DNA concentration 38\u003c\/p\u003e \u003cp\u003e3.1.6 Other methods for the preparation of total cell DNA 39\u003c\/p\u003e \u003cp\u003e3.2 Preparation of plasmid DNA 40\u003c\/p\u003e \u003cp\u003e3.2.1 Separation on the basis of size 41\u003c\/p\u003e \u003cp\u003e3.2.2 Separation on the basis of conformation 42\u003c\/p\u003e \u003cp\u003e3.2.3 Plasmid amplification 44\u003c\/p\u003e \u003cp\u003e3.3 Preparation of bacteriophage DNA 46\u003c\/p\u003e \u003cp\u003e3.3.1 Growth of cultures to obtain a high λ titre 47\u003c\/p\u003e \u003cp\u003e3.3.2 Preparation of non‐lysogenic λ phages 47\u003c\/p\u003e \u003cp\u003e3.3.3 Collection of phages from an infected culture 49\u003c\/p\u003e \u003cp\u003e3.3.4 Purification of DNA from λ phage particles 49\u003c\/p\u003e \u003cp\u003e3.3.5 Purification of M13 DNA causes few problems 49\u003c\/p\u003e \u003cp\u003eFurther reading 51\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Manipulation of Purified DNA 53\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e4.1 The range of DNA manipulative enzymes 55\u003c\/p\u003e \u003cp\u003e4.1.1 Nucleases 55\u003c\/p\u003e \u003cp\u003e4.1.2 Ligases 57\u003c\/p\u003e \u003cp\u003e4.1.3 Polymerases 57\u003c\/p\u003e \u003cp\u003e4.1.4 DNA modifying enzymes 58\u003c\/p\u003e \u003cp\u003e4.2 Enzymes for cutting DNA – restriction endonucleases 59\u003c\/p\u003e \u003cp\u003e4.2.1 The discovery and function of restriction endonucleases 60\u003c\/p\u003e \u003cp\u003e4.2.2 Type II restriction endonucleases cut DNA at specific nucleotide sequences 61\u003c\/p\u003e \u003cp\u003e4.2.3 Blunt ends and sticky ends 62\u003c\/p\u003e \u003cp\u003e4.2.4 The frequency of recognition sequences in a DNA molecule 63\u003c\/p\u003e \u003cp\u003e4.2.5 Performing a restriction digest in the laboratory 64\u003c\/p\u003e \u003cp\u003e4.2.6 Analysing the result of restriction endonuclease cleavage 66\u003c\/p\u003e \u003cp\u003e4.2.7 Estimation of the sizes of DNA molecules 68\u003c\/p\u003e \u003cp\u003e4.2.8 Mapping the positions of different restriction sites in a DNA molecule 69\u003c\/p\u003e \u003cp\u003e4.2.9 Special gel electrophoresis methods for separating larger molecules 70\u003c\/p\u003e \u003cp\u003e4.3 Ligation – joining DNA molecules together 72\u003c\/p\u003e \u003cp\u003e4.3.1 The mode of action of DNA ligase 72\u003c\/p\u003e \u003cp\u003e4.3.2 Sticky ends increase the efficiency of ligation 74\u003c\/p\u003e \u003cp\u003e4.3.3 Putting sticky ends onto a blunt‐ended molecule 74\u003c\/p\u003e \u003cp\u003e4.3.4 Blunt‐end ligation with a DNA topoisomerase 79\u003c\/p\u003e \u003cp\u003eFurther reading 81\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Introduction of DNA into Living Cells 83\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e5.1 Transformation – the uptake of DNA by bacterial cells 85\u003c\/p\u003e \u003cp\u003e5.1.1 Not all species of bacteria are equally efficient at DNA uptake 85\u003c\/p\u003e \u003cp\u003e5.1.2 Preparation of competent \u003ci\u003eE. coli \u003c\/i\u003ecells 86\u003c\/p\u003e \u003cp\u003e5.1.3 Selection for transformed cells 86\u003c\/p\u003e \u003cp\u003e5.2 Identification of recombinants 88\u003c\/p\u003e \u003cp\u003e5.2.1 Recombinant selection with pBR322 – insertional inactivation of an antibiotic resistance gene 89\u003c\/p\u003e \u003cp\u003e5.2.2 Insertional inactivation does not always involve antibiotic resistance 90\u003c\/p\u003e \u003cp\u003e5.3 Introduction of phage DNA into bacterial cells 92\u003c\/p\u003e \u003cp\u003e5.3.1 Transfection 93\u003c\/p\u003e \u003cp\u003e5.3.2 \u003ci\u003eIn vitro \u003c\/i\u003epackaging of λ cloning vectors 93\u003c\/p\u003e \u003cp\u003e5.3.3 Phage infection is visualized as plaques on an agar medium 93\u003c\/p\u003e \u003cp\u003e5.3.4 Identification of recombinant phages 95\u003c\/p\u003e \u003cp\u003e5.4 Introduction of DNA into non‐bacterial cells 97\u003c\/p\u003e \u003cp\u003e5.4.1 Transformation of individual cells 97\u003c\/p\u003e \u003cp\u003e5.4.2 Transformation of whole organisms 99\u003c\/p\u003e \u003cp\u003eFurther reading 99\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Cloning Vectors for \u003ci\u003eE. coli \u003c\/i\u003e101\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e6.1 Cloning vectors based on \u003ci\u003eE. coli \u003c\/i\u003eplasmids 102\u003c\/p\u003e \u003cp\u003e6.1.1 The nomenclature of plasmid cloning vectors 102\u003c\/p\u003e \u003cp\u003e6.1.2 The useful properties of pBR322 102\u003c\/p\u003e \u003cp\u003e6.1.3 The pedigree of pBR322 103\u003c\/p\u003e \u003cp\u003e6.1.4 More sophisticated \u003ci\u003eE. coli \u003c\/i\u003eplasmid cloning vectors 104\u003c\/p\u003e \u003cp\u003e6.2 Cloning vectors based on \u003cb\u003eλ \u003c\/b\u003ebacteriophage 108\u003c\/p\u003e \u003cp\u003e6.2.1 Natural selection was used to isolate modified \u003cb\u003e\u003ci\u003eλ \u003c\/i\u003e\u003c\/b\u003ethat lack certain restriction sites 108\u003c\/p\u003e \u003cp\u003e6.2.2 Segments of the \u003cb\u003e\u003ci\u003eλ \u003c\/i\u003e\u003c\/b\u003egenome can be deleted without impairing viability 108\u003c\/p\u003e \u003cp\u003e6.2.3 Insertion and replacement vectors 110\u003c\/p\u003e \u003cp\u003e6.2.4 Cloning experiments with \u003cb\u003e\u003ci\u003eλ \u003c\/i\u003e\u003c\/b\u003einsertion or replacement vectors 112\u003c\/p\u003e \u003cp\u003e6.2.5 Long DNA fragments can be cloned using a cosmid 113\u003c\/p\u003e \u003cp\u003e6.2.6 \u003cb\u003e\u003ci\u003eλ \u003c\/i\u003e\u003c\/b\u003eand other high‐capacity vectors enable genomic libraries to be constructed 114\u003c\/p\u003e \u003cp\u003e6.3 Cloning vectors for synthesis of single‐stranded DNA 115\u003c\/p\u003e \u003cp\u003e6.3.1 Vectors based on M13 bacteriophage 115\u003c\/p\u003e \u003cp\u003e6.3.2 Hybrid plasmid–M13 vectors 117\u003c\/p\u003e \u003cp\u003e6.4 Vectors for other bacteria 118\u003c\/p\u003e \u003cp\u003eFurther reading 119\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Cloning Vectors for Eukaryotes 121\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e7.1 Vectors for yeast and other fungi 121\u003c\/p\u003e \u003cp\u003e7.1.1 Selectable markers for the 2 μm plasmid 122\u003c\/p\u003e \u003cp\u003e7.1.2 Vectors based on the 2 μm plasmid – yeast episomal plasmids 122\u003c\/p\u003e \u003cp\u003e7.1.3 A YEp may insert into yeast chromosomal DNA 124\u003c\/p\u003e \u003cp\u003e7.1.4 Other types of yeast cloning vector 124\u003c\/p\u003e \u003cp\u003e7.1.5 Artificial chromosomes can be used to clone long pieces of DNA in yeast 126\u003c\/p\u003e \u003cp\u003e7.1.6 Vectors for other yeasts and fungi 129\u003c\/p\u003e \u003cp\u003e7.2 Cloning vectors for higher plants 129\u003c\/p\u003e \u003cp\u003e7.2.1 \u003ci\u003eAgrobacterium tumefaciens \u003c\/i\u003e– nature’s smallest genetic engineer 130\u003c\/p\u003e \u003cp\u003e7.2.2 Cloning genes in plants by direct gene transfer 135\u003c\/p\u003e \u003cp\u003e7.2.3 Attempts to use plant viruses as cloning vectors 137\u003c\/p\u003e \u003cp\u003e7.3 Cloning vectors for animals 138\u003c\/p\u003e \u003cp\u003e7.3.1 Cloning vectors for insects 139\u003c\/p\u003e \u003cp\u003e7.3.2 Cloning in mammals 141\u003c\/p\u003e \u003cp\u003eFurther reading 143\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 How to Obtain a Clone of a Specific Gene 145\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8.1 The problem of selection 146\u003c\/p\u003e \u003cp\u003e8.1.1 There are two basic strategies for obtaining the clone you want 146\u003c\/p\u003e \u003cp\u003e8.2 Direct selection 147\u003c\/p\u003e \u003cp\u003e8.2.1 Marker rescue extends the scope of direct selection 149\u003c\/p\u003e \u003cp\u003e8.2.2 The scope and limitations of marker rescue 150\u003c\/p\u003e \u003cp\u003e8.3 Identification of a clone from a gene library 150\u003c\/p\u003e \u003cp\u003e8.3.1 Gene libraries 151\u003c\/p\u003e \u003cp\u003e8.4 Methods for clone identification 153\u003c\/p\u003e \u003cp\u003e8.4.1 Complementary nucleic acid strands hybridize to each other 154\u003c\/p\u003e \u003cp\u003e8.4.2 Colony and plaque hybridization probing 154\u003c\/p\u003e \u003cp\u003e8.4.3 Examples of the practical use of hybridization probing 157\u003c\/p\u003e \u003cp\u003e8.4.4 Identification methods based on detection of the translation product of the cloned gene 164\u003c\/p\u003e \u003cp\u003eFurther reading 166\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 The Polymerase Chain Reaction 169\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e9.1 PCR in outline 170\u003c\/p\u003e \u003cp\u003e9.2 PCR in more detail 172\u003c\/p\u003e \u003cp\u003e9.2.1 Designing the oligonucleotide primers for a PCR 172\u003c\/p\u003e \u003cp\u003e9.2.2 Working out the correct temperatures to use 174\u003c\/p\u003e \u003cp\u003e9.3 After the PCR: studying PCR products 176\u003c\/p\u003e \u003cp\u003e9.3.1 Gel electrophoresis of PCR products 177\u003c\/p\u003e \u003cp\u003e9.3.2 Cloning PCR products 178\u003c\/p\u003e \u003cp\u003e9.4 Real‐time PCR 180\u003c\/p\u003e \u003cp\u003e9.4.1 Carrying out a real‐time PCR experiment 180\u003c\/p\u003e \u003cp\u003e9.4.2 Real‐time PCR enables the amount of starting material to be quantified 182\u003c\/p\u003e \u003cp\u003e9.4.3 Melting curve analysis enables point mutations to be identified 184\u003c\/p\u003e \u003cp\u003eFurther reading 185\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart II The Applications of Gene Cloning and DNA Analysis in Research 187\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Sequencing Genes and Genomes 189\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e10.1 Chain‐termination DNA sequencing 190\u003c\/p\u003e \u003cp\u003e10.1.1 Chain‐termination sequencing in outline 190\u003c\/p\u003e \u003cp\u003e10.1.2 Not all DNA polymerases can be used for sequencing 192\u003c\/p\u003e \u003cp\u003e10.1.3 Chain‐termination sequencing with \u003ci\u003eTaq \u003c\/i\u003epolymerase 193\u003c\/p\u003e \u003cp\u003e10.1.4 Limitations of chain‐termination sequencing 195\u003c\/p\u003e \u003cp\u003e10.2 Next‐generation sequencing 196\u003c\/p\u003e \u003cp\u003e10.2.1 Preparing a library for an Illumina sequencing experiment 197\u003c\/p\u003e \u003cp\u003e10.2.2 The sequencing phase of an Illumina experiment 199\u003c\/p\u003e \u003cp\u003e10.2.3 Ion semiconductor sequencing 201\u003c\/p\u003e \u003cp\u003e10.2.4 Third‐generation sequencing 201\u003c\/p\u003e \u003cp\u003e10.2.5 Next‐generation sequencing without a DNA polymerase 202\u003c\/p\u003e \u003cp\u003e10.2.6 Directing next‐generation sequencing at specific sets of genes 203\u003c\/p\u003e \u003cp\u003e10.3 How to sequence a genome 205\u003c\/p\u003e \u003cp\u003e10.3.1 Shotgun sequencing of prokaryotic genomes 206\u003c\/p\u003e \u003cp\u003e10.3.2 Sequencing of eukaryotic genomes 209\u003c\/p\u003e \u003cp\u003eFurther reading 215\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Studying Gene Expression and Function 217\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e11.1 Studying the RNA transcript of a gene 218\u003c\/p\u003e \u003cp\u003e11.1.1 Detecting the presence of a transcript in an RNA sample 219\u003c\/p\u003e \u003cp\u003e11.1.2 Transcript mapping by hybridization between gene and RNA 220\u003c\/p\u003e \u003cp\u003e11.1.3 Transcript analysis by primer extension 222\u003c\/p\u003e \u003cp\u003e11.1.4 Transcript analysis by PCR 223\u003c\/p\u003e \u003cp\u003e11.2 Studying the regulation of gene expression 224\u003c\/p\u003e \u003cp\u003e11.2.1 Identifying protein binding sites on a DNA molecule 225\u003c\/p\u003e \u003cp\u003e11.2.2 Identifying control sequences by deletion analysis 230\u003c\/p\u003e \u003cp\u003e11.3 Identifying and studying the translation product of a cloned gene 232\u003c\/p\u003e \u003cp\u003e11.3.1 HRT and HART can identify the translation product of a cloned gene 233\u003c\/p\u003e \u003cp\u003e11.3.2 Analysis of proteins by \u003ci\u003ein vitro \u003c\/i\u003emutagenesis 234\u003c\/p\u003e \u003cp\u003eFurther reading 240\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Studying Genomes 243\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e12.1 Locating the genes in a genome sequence 244\u003c\/p\u003e \u003cp\u003e12.1.1 Locating protein‐coding genes by scanning a genome sequence 244\u003c\/p\u003e \u003cp\u003e12.1.2 Gene location is aided by homology searching 247\u003c\/p\u003e \u003cp\u003e12.1.3 Locating genes for noncoding RNA transcripts 249\u003c\/p\u003e \u003cp\u003e12.1.4 Identifying the binding sites for regulatory proteins in a genome sequence 250\u003c\/p\u003e \u003cp\u003e12.2 Determining the function of an unknown gene 251\u003c\/p\u003e \u003cp\u003e12.2.1 Assigning gene functions by computer analysis 251\u003c\/p\u003e \u003cp\u003e12.2.2 Assigning gene function by experimental analysis 252\u003c\/p\u003e \u003cp\u003e12.3 Genome browsers 256\u003c\/p\u003e \u003cp\u003eFurther reading 257\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Studying Transcriptomes and Proteomes 259\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e13.1 Studying transcriptomes 259\u003c\/p\u003e \u003cp\u003e13.1.1 Studying transcriptomes by microarray or chip analysis 260\u003c\/p\u003e \u003cp\u003e13.1.2 Studying transcriptomes by RNA sequencing 261\u003c\/p\u003e \u003cp\u003e13.2 Studying proteomes 265\u003c\/p\u003e \u003cp\u003e13.2.1 Protein profiling 266\u003c\/p\u003e \u003cp\u003e13.2.2 Studying protein–protein interactions 270\u003c\/p\u003e \u003cp\u003eFurther reading 274\u003c\/p\u003e \u003cp\u003e\u003cb\u003ePart III The Applications of Gene Cloning and DNA Analysis in Biotechnology 275\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 Production of Protein from Cloned Genes 277\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e14.1 Special vectors for expression of foreign genes in \u003ci\u003eE. coli \u003c\/i\u003e280\u003c\/p\u003e \u003cp\u003e14.1.1 The promoter is the critical component of an expression vector 281\u003c\/p\u003e \u003cp\u003e14.1.2 Cassettes and gene fusions 285\u003c\/p\u003e \u003cp\u003e14.2 General problems with the production of recombinant protein in \u003ci\u003eE. coli \u003c\/i\u003e287\u003c\/p\u003e \u003cp\u003e14.2.1 Problems resulting from the sequence of the foreign gene 288\u003c\/p\u003e \u003cp\u003e14.2.2 Problems caused by \u003ci\u003eE. coli \u003c\/i\u003e289\u003c\/p\u003e \u003cp\u003e14.3 Production of recombinant protein by eukaryotic cells 290\u003c\/p\u003e \u003cp\u003e14.3.1 Recombinant protein from yeast and filamentous fungi 291\u003c\/p\u003e \u003cp\u003e14.3.2 Using animal cells for recombinant protein production 293\u003c\/p\u003e \u003cp\u003e14.3.3 Pharming – recombinant protein from live animals and plants 295\u003c\/p\u003e \u003cp\u003eFurther reading 298\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 Gene Cloning and DNA Analysis in Medicine 301\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e15.1 Production of recombinant pharmaceuticals 301\u003c\/p\u003e \u003cp\u003e15.1.1 Recombinant insulin 302\u003c\/p\u003e \u003cp\u003e15.1.2 Synthesis of human growth hormones in \u003ci\u003eE. coli \u003c\/i\u003e304\u003c\/p\u003e \u003cp\u003e15.1.3 Recombinant factor VIII 305\u003c\/p\u003e \u003cp\u003e15.1.4 Synthesis of other recombinant human proteins 308\u003c\/p\u003e \u003cp\u003e15.1.5 Recombinant vaccines 308\u003c\/p\u003e \u003cp\u003e15.2 Identification of genes responsible for human diseases 314\u003c\/p\u003e \u003cp\u003e15.2.1 How to identify a gene for a genetic disease 315\u003c\/p\u003e \u003cp\u003e15.2.2 Genetic typing of disease mutations 320\u003c\/p\u003e \u003cp\u003e15.3 Gene therapy 321\u003c\/p\u003e \u003cp\u003e15.3.1 Gene therapy for inherited diseases 321\u003c\/p\u003e \u003cp\u003e15.3.2 Gene therapy and cancer 323\u003c\/p\u003e \u003cp\u003e15.3.3 The ethical issues raised by gene therapy 324\u003c\/p\u003e \u003cp\u003eFurther reading 325\u003c\/p\u003e \u003cp\u003e\u003cb\u003e16 Gene Cloning and DNA Analysis in Agriculture 327\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e16.1 The gene addition approach to plant genetic engineering 328\u003c\/p\u003e \u003cp\u003e16.1.1 Plants that make their own insecticides 328\u003c\/p\u003e \u003cp\u003e16.1.2 Herbicide‐resistant crops 334\u003c\/p\u003e \u003cp\u003e16.1.3 Improving the nutritional quality of plants by gene addition 337\u003c\/p\u003e \u003cp\u003e16.1.4 Other gene addition projects 338\u003c\/p\u003e \u003cp\u003e16.2 Gene subtraction 339\u003c\/p\u003e \u003cp\u003e16.2.1 Antisense RNA and the engineering of fruit ripening in tomato 340\u003c\/p\u003e \u003cp\u003e16.2.2 Other examples of the use of antisense RNA in plant genetic engineering 342\u003c\/p\u003e \u003cp\u003e16.3 Gene editing with a programmable nuclease 344\u003c\/p\u003e \u003cp\u003e16.3.1 Gene editing of phytoene desaturase in rice 344\u003c\/p\u003e \u003cp\u003e16.3.2 Editing of multiple genes in a single plant 346\u003c\/p\u003e \u003cp\u003e16.3.3 Future developments in gene editing of plants 347\u003c\/p\u003e \u003cp\u003e16.4 Are GM plants harmful to human health and the environment? 349\u003c\/p\u003e \u003cp\u003e16.4.1 Safety concerns with selectable markers 349\u003c\/p\u003e \u003cp\u003e16.4.2 The possibility of harmful effects on the environment 350\u003c\/p\u003e \u003cp\u003eFurther reading 351\u003c\/p\u003e \u003cp\u003e\u003cb\u003e17 Gene Cloning and DNA Analysis in Forensic Science and Archaeology 355\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e17.1 DNA analysis in the identification of crime suspects 356\u003c\/p\u003e \u003cp\u003e17.1.1 Genetic fingerprinting by hybridization probing 356\u003c\/p\u003e \u003cp\u003e17.1.2 DNA profiling by PCR of short tandem repeats 357\u003c\/p\u003e \u003cp\u003e17.2 Studying kinship by DNA profiling 359\u003c\/p\u003e \u003cp\u003e17.2.1 Related individuals have similar DNA profiles 359\u003c\/p\u003e \u003cp\u003e17.2.2 DNA profiling and the remains of the Romanovs 360\u003c\/p\u003e \u003cp\u003e17.3 Sex identification by DNA analysis 363\u003c\/p\u003e \u003cp\u003e17.3.1 PCRs directed at Y chromosome‐specific sequences 363\u003c\/p\u003e \u003cp\u003e17.3.2 PCR of the amelogenin gene 364\u003c\/p\u003e \u003cp\u003e17.4 Archaeogenetics – using DNA to study human prehistory 365\u003c\/p\u003e \u003cp\u003e17.4.1 The origins of modern humans 365\u003c\/p\u003e \u003cp\u003e17.4.2 DNA can also be used to study prehistoric human migrations 370\u003c\/p\u003e \u003cp\u003eFurther reading 374\u003c\/p\u003e \u003cp\u003eGlossary 377\u003c\/p\u003e \u003cp\u003eIndex 395\u003c\/p\u003e  \u003cp\u003e\u003cb\u003eT. A. Brown\u003c\/b\u003e is Emeritus Professor of Biomolecular Archaeology in the Manchester Institute of Biotechnology at the University of Manchester in the United Kingdom. He has published several books on genetics, genomics and biochemistry as well as over 150 research papers.   \u003c\/p\u003e\u003cp\u003e\u003cb\u003eKnown worldwide as the standard introductory text to cloning and DNA analysis in Genetics\u003c\/b\u003e \u003c\/p\u003e\u003cp\u003eThe eighth edition of this well-recognised textbook preserves the tradition of excellence created by previous editions. Comprehensive and authoritative, the book explores all of the topics crucial to an understanding of gene cloning in an approachable way. An easy-to-follow and user-friendly layout is presented in full-colour throughout the volume, making it simple to absorb the clear and accessible material contained within. \u003c\/p\u003e\u003cp\u003e\u003ci\u003eGene Cloning and DNA Analysis: An Introduction, Eighth Edition\u003c\/i\u003e contains updated and extended coverage of gene editing strategies like CRISPR\/Cas, rewritten chapters on DNA sequencing and genome studies, as well as new material on real-time PCR and typing of human disease mutations. Over 250 full-colour illustrations are included to bring to life the comprehensive content. The book also covers topics like: \u003c\/p\u003e\u003cul\u003e \u003cli\u003eThe strategies used by researchers and industry practitioners to assemble genome sequences\u003c\/li\u003e \u003cli\u003eNext-Generation Sequencing methods and descriptions of their applications in studying genomes and transcriptomes\u003c\/li\u003e \u003cli\u003eIncludes the use and application of gene editing strategies\u003c\/li\u003e \u003cli\u003eInterbreeding between Neanderthals and Homo sapiens\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003e\u003ci\u003eGene Cloning and DNA Analysis: An Introduction, Eighth Edition\u003c\/i\u003e is an invaluable introductory text for students in classes like genetics and genomics, molecular biology, biochemistry, immunology, and applied biology. It also belongs on the bookshelves of every professional who desires to improve their understanding of the basics of gene cloning or DNA analysis.\u003c\/p\u003e","brand":"Wiley-Blackwell","offers":[{"title":"Default Title","offer_id":47989276115173,"sku":"NP9781119640783","price":58.5,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9781119640783.jpg?v=1761783481","url":"https:\/\/k12savings.com\/es\/products\/gene-cloning-and-dna-analysis-isbn-9781119640783","provider":"K12savings","version":"1.0","type":"link"}