{"product_id":"g-protein-coupled-receptors-isbn-9780470749142","title":"G Protein-Coupled Receptors","description":"G-Protein Coupled Receptors (GPCRs) are not only the largest protein family in the human genome but are also the single biggest target for therapeutic agents. Research into GPCRs is therefore growing at a fast pace and the range of techniques that can be applied to GPCRs is vast and continues to grow. This book provides an invaluable bench-side guide into the best and most up-to-date techniques for current and future research on GPCRs.  \u003cp\u003eWith contributions from leading international authorities, this book equips readers with clear and detailed protocols for both well-known and up-and-coming techniques along with hints and tips for success. All the methods have been tried and tested by leading international research labs and are presented in easy-to-follow stages along with a useful overview of each technique.\u003c\/p\u003e \u003cp\u003eThis book is an essential resource for all researchers in molecular biology, biochemistry, pharmacology and for graduate students.\u003c\/p\u003e  \u003cb\u003ePreface.\u003c\/b\u003e  \u003cp\u003e\u003cb\u003eContributors.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Measurement of Ligand–G-Protein-coupled Receptor Interactions (\u003c\/b\u003e\u003ci\u003eKatie Leach, Celine Valant, Patrick M. Sexton and Arthur Christopoulos\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e1.1 Introduction.\u003c\/p\u003e \u003cp\u003e1.2 Methods and Approaches.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Second Messenger Assays for G-Protein-coupled Receptors: cAMP, Ca\u003csup\u003e2+,\u003c\/sup\u003e Inositol Phosphates\u003csup\u003e\u003csub\u003e, ERK1\/2\u003c\/sub\u003e (\u003c\/sup\u003e\u003ci\u003eKaren J. Gregory, Patrick M. Sexton, Arthur Christopoulos and Caroline A. Hick\u003c\/i\u003e).\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2.1 Introduction.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2.2 Methods and Approaches.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2.3 Troubleshooting.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eReferences.\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Use of the [\u003csup\u003e35\u003c\/sup\u003eS]GTP\u003c\/b\u003eγ\u003cb\u003eS Binding Assay to Determine Ligand Efficacy at G-protein-coupled Receptors (\u003c\/b\u003e\u003ci\u003eElodie Kara and Philip G. Strange\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e3.1 Introduction.\u003c\/p\u003e \u003cp\u003e3.2 Methods and Approaches.\u003c\/p\u003e \u003cp\u003e3.3 Troubleshooting.\u003c\/p\u003e \u003cp\u003eAcknowledgements.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 Quantitative Imaging of Receptor Trafficking\u003c\/b\u003e (\u003ci\u003eAndy R. James, Takeo Awaji, F. Anne Stephenson and Nicholas A. Hartell\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e4.1 Introduction.\u003c\/p\u003e \u003cp\u003e4.2 Methods and Approaches.\u003c\/p\u003e \u003cp\u003e4.3 Troubleshooting.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 Production of Recombinant G-Protein-coupled Receptor in Yeast for Structural and Functional Analysis\u003c\/b\u003e (\u003ci\u003eRichard A.J. Darby, Mohammed Jamshad, Ljuban Grgic, William J. Holmes and Roslyn M. Bill\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e5.1 Introduction.\u003c\/p\u003e \u003cp\u003e5.2 Methods and Approaches.\u003c\/p\u003e \u003cp\u003e5.3 Troubleshooting.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Monitoring GPCR–Protein Complexes Using Bioluminescence Resonance Energy Transfer\u003c\/b\u003e (\u003ci\u003eWerner C. Jaeger, Kevin D.G. Pfleger and Karin A. Eidne\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e6.1 Introduction.\u003c\/p\u003e \u003cp\u003e6.2 Methods and Approaches.\u003c\/p\u003e \u003cp\u003e6.3 Troubleshooting.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 Using Intramolecular Fluorescence Resonance Energy Transfer to Study Receptor Conformation (\u003c\/b\u003e\u003ci\u003eCornelius Krasel and Carsten Hoffmann\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e7.1 Introduction.\u003c\/p\u003e \u003cp\u003e7.2 Methods and Approaches.\u003c\/p\u003e \u003cp\u003e7.3 Troubleshooting.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 A Disulfide Cross-linking Strategy Useful for Studying Ligand-induced Structural Changes in GPCRs\u003c\/b\u003e (\u003ci\u003eJian Hua Li, Stuart D.C. Ward, Sung-Jun Han, Fadi F. Hamdan and Jürgen Wess\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e8.1 Introduction.\u003c\/p\u003e \u003cp\u003e8.2 Methods and Approaches.\u003c\/p\u003e \u003cp\u003e8.3 General Considerations, Caveats and Troubleshooting.\u003c\/p\u003e \u003cp\u003eAcknowledgements.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Use of Fluorescence Correlation Spectroscopy to Study the Diffusion of G-protein-coupled Receptors\u003c\/b\u003e (\u003ci\u003eStephen J. Briddon, Jonathan A. Hern and Stephen J. Hill\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e9.1 Introduction.\u003c\/p\u003e \u003cp\u003e9.2 Methods and Approaches.\u003c\/p\u003e \u003cp\u003e9.3 Troubleshooting.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Identification and Proteomic Analysis of GPCR Phosphorylation\u003c\/b\u003e (\u003ci\u003eKok Choi Kong, Sharad C. Mistry and Andrew B. Tobin\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e10.1 Introduction.\u003c\/p\u003e \u003cp\u003e10.2 Methods.\u003c\/p\u003e \u003cp\u003eAcknowledgements.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Measurement and Visualization of G-Protein-coupled Receptor Trafficking by Enzyme-linked Immunosorbent Assay and Immunofluorescence\u003c\/b\u003e (\u003ci\u003eStuart J. Mundell, Shaista P. Nisar and Eamonn Kelly\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e11.1 Introduction.\u003c\/p\u003e \u003cp\u003e11.2 Methods and Approaches.\u003c\/p\u003e \u003cp\u003e11.3 Troubleshooting.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Substituted Cysteine Accessibility Method (SCAM)\u003c\/b\u003e (\u003ci\u003eGeorge Liapakis and Jonathan A. Javitch\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e12.1 Introduction.\u003c\/p\u003e \u003cp\u003e12.2 Methods and Approaches.\u003c\/p\u003e \u003cp\u003e12.3 Troubleshooting.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Homology Modelling of G-Protein-coupled Receptors\u003c\/b\u003e (\u003ci\u003eJohn Simms\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003e13.1 Introduction.\u003c\/p\u003e \u003cp\u003e13.2 Methods and Approaches.\u003c\/p\u003e \u003cp\u003e13.3 Troubleshooting.\u003c\/p\u003e \u003cp\u003e13.4 Automated Methods for Generating Models of GPCRs.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003e\u003cb\u003eAppendix: Site-directed Mutagenesis and Chimeras (\u003c\/b\u003e\u003ci\u003eAlex Conner, Mark Wheatley and David Poyner\u003c\/i\u003e).\u003c\/p\u003e \u003cp\u003eA.1 Introduction.\u003c\/p\u003e \u003cp\u003eA.2 Why Mutagenesis?\u003c\/p\u003e \u003cp\u003eA.3 Troubleshooting.\u003c\/p\u003e \u003cp\u003eA.4 Conclusion.\u003c\/p\u003e \u003cp\u003eReferences.\u003c\/p\u003e \u003cp\u003eIndex. \u003c\/p\u003e  \u003cp\u003e\u003cstrong\u003eDr David R. Poyner\u003c\/strong\u003e, University of Aston, Birmingham, UK; School of Life and Health Sciences, Aston University, Birmingham, UK. \u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eProfessor?Mark Wheatley\u003c\/strong\u003e, University of Birmingham, UK.   G Protein Coupled Receptors (GPCRs) are not only the largest protein family in the human genome but are also the single biggest target for therapeutic agents. Research into GPCRs is therefore growing at a fast pace and the range of techniques that can be applied to GPCRs is vast and continues to grow. This book provides an invaluable bench-side guide into the best and most up-to-date techniques for current and future research on GPCRs.  \u003c\/p\u003e\u003cp\u003eWith contributions from leading international authorities, \u003ci\u003eGPCR Essential Methods\u003c\/i\u003e equips readers with clear and detailed protocols for both well-known and up-and-coming techniques along with hints and tips for success. All the methods have been tried and tested by leading international research labs and are presented in easy-to-follow stages along with a useful overview of each technique.\u003c\/p\u003e \u003cp\u003eThis book will be of interest to researchers in molecular biology, biochemistry, pharmacology and will be a useful resource for graduate students.\u003c\/p\u003e \u003cul\u003e \u003cli\u003eHighlights the best and most up-to-date techniques for working on GPCRs from the vast range currently in use\u003c\/li\u003e \u003cli\u003eExplains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches\u003c\/li\u003e \u003cli\u003eChapters are written by leading international authorities in the field and cover both new and well-known, tried and tested methods for working with GPCRs\u003c\/li\u003e \u003cli\u003eAn essential laboratory manual for researchers at all levels\u003cbr\u003e \u003c\/li\u003e \u003c\/ul\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47989269266661,"sku":"NP9780470749142","price":123.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9780470749142.jpg?v=1761783454","url":"https:\/\/k12savings.com\/es\/products\/g-protein-coupled-receptors-isbn-9780470749142","provider":"K12savings","version":"1.0","type":"link"}