{"product_id":"dna-methods-in-food-safety-isbn-9781118278673","title":"DNA Methods in Food Safety","description":"\u003cp\u003eMolecular typing of foodborne pathogens has become an indispensable tool in epidemiological studies. Thanks to these techniques, we now have a better understanding of the distribution and appearance of bacterial foodborne diseases and have a deeper knowledge of the type of food products associated with the major foodborne pathogens. Within the molecular techniques, DNA-based techniques have prospered for more than 40 years and have been incorporated in the first surveillance systems to monitor bacterial foodborne pathogens in the United States and other countries. However, DNA techniques vary widely and many microbiology laboratory personnel working with food and\/or water face the dilemma of which method to incorporate.\u003c\/p\u003e \u003cp\u003e\u003ci\u003eDNA Methods in Food Safety: Molecular Typing of Foodborne and Waterborne Bacterial Pathogens\u003c\/i\u003e succinctly reviews more than 25 years of data on a variety of DNA typing techniques, summarizing the different mathematical models for analysis and interpretation of results, and detailing their efficacy in typing different foodborne and waterborne bacterial pathogens, such as \u003ci\u003eCampylobacter, Clostridium perfringens, Listeria, Salmonella,\u003c\/i\u003e among others. Section I describes the different DNA techniques used in the typing of bacterial foodborne pathogens, whilst Section II deals with the application of these techniques to type the most important bacterial foodborne pathogens. In Section II the emphasis is placed on the pathogen, and each chapter describes some of the most appropriate techniques for typing each bacterial pathogen.\u003c\/p\u003e \u003cp\u003eThe techniques presented in this book are the most significant in the study of the molecular epidemiology of bacterial foodborne pathogens to date. It therefore provides a unique reference for students and professionals in the field of microbiology, food and water safety and epidemiology and molecular epidemiology.\u003c\/p\u003e  \u003cp\u003eList of Contributors vii\u003c\/p\u003e \u003cp\u003ePreface xiii\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSection I Typing Method, Analysis, and Applications 1\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e1 Polymerase Chain Reaction-Based Subtyping Methods 3\u003cbr\u003e \u003ci\u003eYi Chen and Insook Son\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2 Pulsed-Field Gel Electrophoresis and the Molecular Epidemiology of Foodborne Pathogens 27\u003cbr\u003e \u003ci\u003eMohana Ray and David C. Schwartz\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3 Multilocus Sequence Typing: An Adaptable Tool for Understanding the Global Epidemiology of Bacterial Pathogens 47\u003cbr\u003e \u003ci\u003eStephen J. Knabel\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e4 High-Throughput Sequencing 65\u003cbr\u003e \u003ci\u003eXiangyu Deng, Lee S. Katz, Patricia I. Fields, and Wei Zhang\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5 Analysis of Typing Results 85\u003cbr\u003e \u003ci\u003eJoão André Carriço and Mário Ramirez\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6 Databases and Internet Applications 113\u003cbr\u003e \u003ci\u003eG. Gopinath, K. Hari, R. Jain, M. H. Kothary, K. G. Jarvis, A. A. Franco, C. J. Grim, V. Sathyamoorthy, M. K. Mammel, A. R. Datta, B. A. McCardell, M. D. Solomotis, and Ben D. Tall\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7 The Transformation of Disease Surveillance, Outbreak Detection, and Regulatory Response by Molecular Epidemiology 133\u003cbr\u003e \u003ci\u003eDavid A. Sweat\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003cb\u003eSection II Pathogens 163\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e8 The Genus Bacillus 165\u003cbr\u003e \u003ci\u003eMonika Ehling-Schulz and Ute Messelhäusser\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e9 Molecular Typing of Campylobacter jejuni 185\u003cbr\u003e \u003ci\u003eCatherine D. Carrillo and Omar A. Oyarzabal\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e10 DNA Typing Methods for Members of the Cronobacter Genus 205\u003cbr\u003e \u003ci\u003eSusan Joseph and Stephen Forsythe\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e11 Molecular Subtyping Approaches for Pathogenic Clostridium spp. Isolated from Foods 249\u003cbr\u003e \u003ci\u003eBrian H. Raphael, Deborah F. Talkington, Carolina Lúquez, and Susan E. Maslanka\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e12 Molecular Characterization of Shiga Toxin-Producing Escherichia coli 275\u003cbr\u003e \u003ci\u003ePallavi Singh and Shannon D. Manning\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e13 Molecular Subtyping Methods for Listeria monocytogenes: Tools for Tracking and Control 303\u003cbr\u003e \u003ci\u003eSara Lomonaco and Daniele Nucera\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e14 Salmonella 337\u003cbr\u003e \u003ci\u003eAaron M. Lynne, Jing Han, and Steven L. Foley\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e15 Vibrio cholerae 359\u003cbr\u003e \u003ci\u003eDong Wook Kim\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003eIndex 381\u003c\/p\u003e \u003cp\u003e\u003cb\u003eDr Omar A. Oyarzabal \u003c\/b\u003eis Vice President of Technical Services at IEH Laboratories and Consulting Group, Seattle, WA\u003c\/p\u003e \u003cp\u003e\u003cb\u003eDr Sophia Kathariou\u003c\/b\u003e is Professor of Bioprocessing and Nutrition Sciences at the Department of Food, North Carolina State University, Raleigh, USA\u003c\/p\u003e \u003cp\u003eMolecular typing of foodborne pathogens has become an indispensable tool in epidemiological studies. Thanks to these techniques, we now have a better understanding of the distribution and appearance of bacterial foodborne diseases and have a deeper knowledge of the type of food products associated with the major foodborne pathogens. Within the molecular techniques, DNA-based techniques have prospered for more than 40 years and have been incorporated in the first surveillance systems to monitor bacterial foodborne pathogens in the United States and other countries. However, DNA techniques vary widely and many microbiology laboratory personnel working with food and\/or water face the dilemma of which method to incorporate.\u003c\/p\u003e \u003cp\u003e\u003ci\u003eDNA Methods in Food Safety: Molecular Typing of Foodborne and Waterborne Bacterial Pathogens\u003c\/i\u003e succinctly reviews more than 25 years of data on a variety of DNA typing techniques, summarizing the different mathematical models for analysis and interpretation of results, and detailing their efficacy in typing different foodborne and waterborne bacterial pathogens, such as \u003ci\u003eCampylobacter, Clostridium perfringens, Listeria, Salmonella,\u003c\/i\u003e among others. Section I describes the different DNA techniques used in the typing of bacterial foodborne pathogens, whilst Section II deals with the application of these techniques to type the most important bacterial foodborne pathogens. In Section II the emphasis is placed on the pathogen, and each chapter describes some of the most appropriate techniques for typing each bacterial pathogen.\u003c\/p\u003e \u003cp\u003eThe techniques presented in this book are the most significant in the study of the molecular epidemiology of bacterial foodborne pathogens to date. It therefore provides a unique reference for students and professionals in the field of microbiology, food and water safety and epidemiology and molecular epidemiology.\u003c\/p\u003e","brand":"Wiley-Blackwell","offers":[{"title":"Default Title","offer_id":47989079703781,"sku":"NP9781118278673","price":192.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9781118278673.jpg?v=1761782710","url":"https:\/\/k12savings.com\/es\/products\/dna-methods-in-food-safety-isbn-9781118278673","provider":"K12savings","version":"1.0","type":"link"}