{"product_id":"diagnostic-electron-microscopy-isbn-9781119973997","title":"Diagnostic Electron Microscopy","description":"\u003cp\u003e\u003cb\u003eDiagnostic Electron Microscopy\u003c\/b\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003e\u003cb\u003eDiagnostic Electron Microscopy: A Practical Guide to Interpretation and Technique\u003c\/b\u003e\u003c\/i\u003e summarises the current interpretational applications of TEM in diagnostic pathology. This concise and accessible volume provides a working guide to the main, or most useful, applications of the technique including practical topics of concern to laboratory scientists, brief guides to traditional tissue and microbiological preparation techniques, microwave processing, digital imaging and measurement uncertainty.\u003c\/p\u003e \u003cp\u003eThe text features both a screening and interpretational guide for TEM diagnostic applications and current TEM diagnostic tissue preparation methods pertinent to all clinical electron microscope units worldwide. Containing high-quality representative images, this up-to-date text includes detailed information on the most important diagnostic applications of transmission electron microscopy as well as instructions for specific tissues and current basic preparative techniques.\u003c\/p\u003e \u003cp\u003eThe book is relevant to trainee pathologists and practising pathologists who are expected to understand and evaluate\/screen tissues by TEM. In addition, technical and scientific staff involved in tissue preparation and diagnostic tissue evaluation\/screening by TEM will find this text useful.\u003c\/p\u003e \u003cp\u003eList of Contributors xvii\u003c\/p\u003e \u003cp\u003ePreface – Introduction xxi\u003c\/p\u003e \u003cp\u003e\u003cb\u003e1 Renal Disease 1\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJohn W. Stirling and Alan Curry\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e1.1 The Role of Transmission Electron Microscopy (TEM) in Renal Diagnostics 1\u003c\/p\u003e \u003cp\u003e1.2 Ultrastructural Evaluation and Interpretation 2\u003c\/p\u003e \u003cp\u003e1.3 The Normal Glomerulus 3\u003c\/p\u003e \u003cp\u003e1.3.1 The Glomerular Basement Membrane 4\u003c\/p\u003e \u003cp\u003e1.4 Ultrastructural Diagnostic Features 5\u003c\/p\u003e \u003cp\u003e1.4.1 Deposits: General Features 5\u003c\/p\u003e \u003cp\u003e1.4.2 Granular and Amorphous Deposits 6\u003c\/p\u003e \u003cp\u003e1.4.3 Organised Deposits: Fibrils and Tubules 7\u003c\/p\u003e \u003cp\u003e1.4.4 Nonspecific Fibrils 11\u003c\/p\u003e \u003cp\u003e1.4.5 General and Nonspecific Inclusions and Deposits 11\u003c\/p\u003e \u003cp\u003e1.4.6 Fibrin 12\u003c\/p\u003e \u003cp\u003e1.4.7 Tubuloreticular Bodies (Tubuloreticular Inclusions) 12\u003c\/p\u003e \u003cp\u003e1.4.8 The Glomerular Basement Membrane 13\u003c\/p\u003e \u003cp\u003e1.4.9 The Mesangial Matrix 14\u003c\/p\u003e \u003cp\u003e1.4.10 Cellular Components of the Glomerulus 14\u003c\/p\u003e \u003cp\u003e1.4.11 Parietal Epithelium 16\u003c\/p\u003e \u003cp\u003e1.5 The Ultrastructural Pathology of the Major Glomerular Diseases 16\u003c\/p\u003e \u003cp\u003e1.5.1 Diseases without, or with Only Minor, Structural GBM Changes 16\u003c\/p\u003e \u003cp\u003e1.5.2 Diseases with Structural GBM Changes 19\u003c\/p\u003e \u003cp\u003e1.5.3 Diseases with Granular Deposits 25\u003c\/p\u003e \u003cp\u003e1.5.4 Diseases with Organised Deposits 40\u003c\/p\u003e \u003cp\u003e1.5.5 Hereditary Metabolic Storage Disorders 46\u003c\/p\u003e \u003cp\u003eReferences 47\u003c\/p\u003e \u003cp\u003e\u003cb\u003e2 Transplant Renal Biopsies 55\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJohn Brealey\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e2.1 Introduction 55\u003c\/p\u003e \u003cp\u003e2.2 The Transplant Renal Biopsy 55\u003c\/p\u003e \u003cp\u003e2.3 Indications for Electron Microscopy of Transplant Kidney 56\u003c\/p\u003e \u003cp\u003e2.3.1 Transplant Glomerulopathy 56\u003c\/p\u003e \u003cp\u003e2.3.2 Recurrent Primary Disease 64\u003c\/p\u003e \u003cp\u003e2.3.3 De Novo Glomerular Disease 72\u003c\/p\u003e \u003cp\u003e2.3.4 Donor-Related Disease 74\u003c\/p\u003e \u003cp\u003e2.3.5 Infection 74\u003c\/p\u003e \u003cp\u003e2.3.6 Inconclusive Diagnosis by LM and\/or IM 79\u003c\/p\u003e \u003cp\u003e2.3.7 Miscellaneous Topics 81\u003c\/p\u003e \u003cp\u003eReferences 84\u003c\/p\u003e \u003cp\u003e\u003cb\u003e3 Electron Microscopy in Skeletal Muscle Pathology 89\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eElizabeth Curtis and Caroline Sewry\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e3.1 Introduction 89\u003c\/p\u003e \u003cp\u003e3.1.1 The Biopsy Procedure 90\u003c\/p\u003e \u003cp\u003e3.1.2 Sampling 90\u003c\/p\u003e \u003cp\u003e3.1.3 Tissue Processing 90\u003c\/p\u003e \u003cp\u003e3.1.4 Artefacts 91\u003c\/p\u003e \u003cp\u003e3.2 Normal Muscle 91\u003c\/p\u003e \u003cp\u003e3.3 Pathological Changes 96\u003c\/p\u003e \u003cp\u003e3.3.1 Sarcolemma 96\u003c\/p\u003e \u003cp\u003e3.3.2 Myofibrils 99\u003c\/p\u003e \u003cp\u003e3.3.3 Glycogen 102\u003c\/p\u003e \u003cp\u003e3.3.4 Cores 104\u003c\/p\u003e \u003cp\u003e3.3.5 Target Fibres 105\u003c\/p\u003e \u003cp\u003e3.3.6 Myonuclei 105\u003c\/p\u003e \u003cp\u003e3.3.7 Mitochondria 106\u003c\/p\u003e \u003cp\u003e3.3.8 Reticular System 108\u003c\/p\u003e \u003cp\u003e3.3.9 Vacuoles 109\u003c\/p\u003e \u003cp\u003e3.3.10 Capillaries 110\u003c\/p\u003e \u003cp\u003e3.3.11 Other Structural Defects 111\u003c\/p\u003e \u003cp\u003eReferences 113\u003c\/p\u003e \u003cp\u003e\u003cb\u003e4 The Diagnostic Electron Microscopy of Nerve 117\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eRosalind King\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e4.1 Introduction 117\u003c\/p\u003e \u003cp\u003e4.2 Tissue Processing 118\u003c\/p\u003e \u003cp\u003e4.2.1 Preparation of Nerve Biopsy Specimens 118\u003c\/p\u003e \u003cp\u003e4.3 Normal Nerve Ultrastructure 120\u003c\/p\u003e \u003cp\u003e4.3.1 Axons 120\u003c\/p\u003e \u003cp\u003e4.3.2 Schwann Cells 120\u003c\/p\u003e \u003cp\u003e4.3.3 The Myelin Sheath 120\u003c\/p\u003e \u003cp\u003e4.3.4 Node of Ranvier 122\u003c\/p\u003e \u003cp\u003e4.3.5 Paranode 123\u003c\/p\u003e \u003cp\u003e4.3.6 Juxtaparanode 123\u003c\/p\u003e \u003cp\u003e4.3.7 Internode 123\u003c\/p\u003e \u003cp\u003e4.3.8 Schmidt–Lanterman Incisures 124\u003c\/p\u003e \u003cp\u003e4.3.9 Remak Fibres 124\u003c\/p\u003e \u003cp\u003e4.3.10 Fibroblasts 124\u003c\/p\u003e \u003cp\u003e4.3.11 Renaut Bodies 125\u003c\/p\u003e \u003cp\u003e4.4 Pathological Ultrastructural Features 125\u003c\/p\u003e \u003cp\u003e4.4.1 Axonal Degeneration 125\u003c\/p\u003e \u003cp\u003e4.4.2 Axonal Regeneration 126\u003c\/p\u003e \u003cp\u003e4.4.3 Remak Fibre Abnormalities 128\u003c\/p\u003e \u003cp\u003e4.4.4 Polyglucosan Bodies 128\u003c\/p\u003e \u003cp\u003e4.4.5 Nonspecific Axonal Inclusions 128\u003c\/p\u003e \u003cp\u003e4.4.6 Demyelination and Remyelination 130\u003c\/p\u003e \u003cp\u003e4.4.7 Specific Schwann Cell Inclusions 135\u003c\/p\u003e \u003cp\u003e4.4.8 Nonspecific Schwann Cell Inclusions 136\u003c\/p\u003e \u003cp\u003e4.4.9 Fibroblasts 142\u003c\/p\u003e \u003cp\u003e4.4.10 Perineurial Abnormalities 142\u003c\/p\u003e \u003cp\u003e4.4.11 Cellular Infiltration 143\u003c\/p\u003e \u003cp\u003e4.4.12 Endoneurial Oedema 143\u003c\/p\u003e \u003cp\u003e4.4.13 Connective Tissue Abnormalities 143\u003c\/p\u003e \u003cp\u003e4.4.14 Endoneurial Blood Vessels 145\u003c\/p\u003e \u003cp\u003e4.4.15 Mast Cells 145\u003c\/p\u003e \u003cp\u003e4.5 Artefact 145\u003c\/p\u003e \u003cp\u003e4.6 Conclusions 147\u003c\/p\u003e \u003cp\u003eReferences 148\u003c\/p\u003e \u003cp\u003e\u003cb\u003e5 The Diagnostic Electron Microscopy of Tumours 153\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eBrian Eyden\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e5.1 Introduction 153\u003c\/p\u003e \u003cp\u003e5.2 Principles and Procedures for Diagnosing Tumours by Electron Microscopy 154\u003c\/p\u003e \u003cp\u003e5.2.1 The Objective of Tumour Diagnosis 154\u003c\/p\u003e \u003cp\u003e5.2.2 The Intellectual Requirements for Tumour Diagnosis by Electron Microscopy 155\u003c\/p\u003e \u003cp\u003e5.2.3 Technical Considerations 156\u003c\/p\u003e \u003cp\u003e5.2.4 Identifying Good Preservation 158\u003c\/p\u003e \u003cp\u003e5.2.5 Distinguishing Reactive from Neoplastic Cells 162\u003c\/p\u003e \u003cp\u003e5.3 Organelles and Groups of Cell Structures Defining Cellular Differentiation 162\u003c\/p\u003e \u003cp\u003e5.3.1 Rough Endoplasmic Reticulum 162\u003c\/p\u003e \u003cp\u003e5.3.2 Melanosomes 165\u003c\/p\u003e \u003cp\u003e5.3.3 Desmosomes 167\u003c\/p\u003e \u003cp\u003e5.3.4 Tonofibrils 167\u003c\/p\u003e \u003cp\u003e5.3.5 Basal Lamina 169\u003c\/p\u003e \u003cp\u003e5.3.6 Glandular Epithelial Differentiation and Cell Processes 171\u003c\/p\u003e \u003cp\u003e5.3.7 Neuroendocrine Granules 171\u003c\/p\u003e \u003cp\u003e5.3.8 Smooth-Muscle Myofilaments 173\u003c\/p\u003e \u003cp\u003e5.3.9 Sarcomeric Myofilaments (Thick-and-Thin Filaments with Z-Disks) 176\u003c\/p\u003e \u003cp\u003eReferences 178\u003c\/p\u003e \u003cp\u003e\u003cb\u003e6 Microbial Ultrastructure 181\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eAlan Curry\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e6.1 Introduction 181\u003c\/p\u003e \u003cp\u003e6.2 Practical Guidance 182\u003c\/p\u003e \u003cp\u003e6.3 Viruses 183\u003c\/p\u003e \u003cp\u003e6.4 Current Use of EM in Virology 185\u003c\/p\u003e \u003cp\u003e6.5 Viruses in Thin Sections of Cells or Tissues 186\u003c\/p\u003e \u003cp\u003e6.6 Bacteria 191\u003c\/p\u003e \u003cp\u003e6.7 Fungal Organisms 194\u003c\/p\u003e \u003cp\u003e6.8 Microsporidia 196\u003c\/p\u003e \u003cp\u003e6.9 Parasitic Protozoa 206\u003c\/p\u003e \u003cp\u003e6.9.1 Cryptosporidium 207\u003c\/p\u003e \u003cp\u003e6.9.2 Isospora belli 211\u003c\/p\u003e \u003cp\u003e6.10 Examples of Non-enteric Protozoa 212\u003c\/p\u003e \u003cp\u003e6.11 Parasitic Amoebae 213\u003c\/p\u003e \u003cp\u003e6.12 Conclusions 214\u003c\/p\u003e \u003cp\u003eAcknowledgements 214\u003c\/p\u003e \u003cp\u003eReferences and Additional Reading 214\u003c\/p\u003e \u003cp\u003e\u003cb\u003e7 The Contemporary Use of Electron Microscopy in the Diagnosis of Ciliary Disorders and Sperm Centriolar Abnormalities 221\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eP. Yiallouros, M. Nearchou, A. Hadjisavvas and K. Kyriacou\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e7.1 Introduction 221\u003c\/p\u003e \u003cp\u003e7.2 Ultrastructure of Motile Cilia 224\u003c\/p\u003e \u003cp\u003e7.3 Genetics of PCD 226\u003c\/p\u003e \u003cp\u003e7.4 Current Diagnostic Modalities 228\u003c\/p\u003e \u003cp\u003e7.5 Clinical Features 229\u003c\/p\u003e \u003cp\u003e7.6 Procurement and Assessment of Ciliated Specimens 230\u003c\/p\u003e \u003cp\u003e7.7 Centriolar Sperm Abnormalities 231\u003c\/p\u003e \u003cp\u003e7.8 Discussion 232\u003c\/p\u003e \u003cp\u003eAcknowledgements 234\u003c\/p\u003e \u003cp\u003eReferences 234\u003c\/p\u003e \u003cp\u003e\u003cb\u003e8 Electron Microscopy as a Useful Tool in the Diagnosis of Lysosomal Storage Diseases 237\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJoseph Alroy, Rolf Pfannl and Angelo A. Ucci\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e8.1 Introduction 237\u003c\/p\u003e \u003cp\u003e8.2 Morphological Findings 247\u003c\/p\u003e \u003cp\u003e8.3 Conclusion 261\u003c\/p\u003e \u003cp\u003eReferences 262\u003c\/p\u003e \u003cp\u003e\u003cb\u003e9 Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy (CADASIL) 269\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJohn W. Stirling\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e9.1 Introduction 269\u003c\/p\u003e \u003cp\u003e9.2 Diagnostic Strategies – Comparative Specificity and Sensitivity 271\u003c\/p\u003e \u003cp\u003e9.3 Diagnosis by TEM 271\u003c\/p\u003e \u003cp\u003eReferences 274\u003c\/p\u003e \u003cp\u003e\u003cb\u003e10 Diagnosis of Platelet Disorders by Electron Microscopy 277\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eHilary Christensen and Walter H.A. Kahr\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e10.1 Introduction 277\u003c\/p\u003e \u003cp\u003e10.2 TEM Preparation of Platelets 278\u003c\/p\u003e \u003cp\u003e10.3 Whole-Mount EM Preparation of Platelets 280\u003c\/p\u003e \u003cp\u003e10.4 EM Preparation of Bone Marrow 281\u003c\/p\u003e \u003cp\u003e10.5 Pre-embed Immunogold Labelling of Von Willibrand Factor in Platelets 282\u003c\/p\u003e \u003cp\u003e10.6 Ultrastructural Features of Platelets 282\u003c\/p\u003e \u003cp\u003e10.7 Normal Platelets 283\u003c\/p\u003e \u003cp\u003e10.8 Grey Platelet Syndrome 285\u003c\/p\u003e \u003cp\u003e10.9 Arthrogryposis, Renal Dysfunction and Cholestasis Syndrome 285\u003c\/p\u003e \u003cp\u003e10.10 Jacobsen Syndrome 285\u003c\/p\u003e \u003cp\u003e10.11 Hermansky–Pudlak Syndrome, Chediak–Higashi Syndrome and Other Dense-Granule Deficiencies 287\u003c\/p\u003e \u003cp\u003e10.12 Type 2B von Willebrand Disease and Platelet-Type von Willebrand Disease 288\u003c\/p\u003e \u003cp\u003eReferences 290\u003c\/p\u003e \u003cp\u003e\u003cb\u003e11 Diagnosis of Congenital Dyserythropoietic Anaemia Types I and II by Transmission Electron Microscopy 293\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eYong-xin Ru\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e11.1 Introduction 293\u003c\/p\u003e \u003cp\u003e11.2 Preparation of Bone Marrow and General Observation Protocol 294\u003c\/p\u003e \u003cp\u003e11.3 CDA Type I 294\u003c\/p\u003e \u003cp\u003e11.3.1 Proerythroblasts and Basophilic Erythroblasts 294\u003c\/p\u003e \u003cp\u003e11.3.2 Polychromatic and Orthochromatic Erythroblasts 295\u003c\/p\u003e \u003cp\u003e11.3.3 Reticulocytes and Erythrocytes 299\u003c\/p\u003e \u003cp\u003e11.4 CDA Type II 299\u003c\/p\u003e \u003cp\u003e11.4.1 Erythroblasts 301\u003c\/p\u003e \u003cp\u003e11.4.2 Erythrocytes 306\u003c\/p\u003e \u003cp\u003e11.5 Summary 306\u003c\/p\u003e \u003cp\u003eAcknowledgements 307\u003c\/p\u003e \u003cp\u003eReferences 307\u003c\/p\u003e \u003cp\u003e\u003cb\u003e12 Ehlers–Danlos Syndrome 309\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eTrinh Hermanns-L\u003c\/i\u003e\u003ci\u003eê, Marie-Annick Reginster, Claudine Pi\u003c\/i\u003e\u003ci\u003eérard-Franchimont and G\u003ci\u003eé\u003c\/i\u003erald E. Pi\u003c\/i\u003e\u003ci\u003eérard\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e12.1 Introduction 309\u003c\/p\u003e \u003cp\u003e12.2 Collagen Fibrils 310\u003c\/p\u003e \u003cp\u003e12.3 Elastic Fibers 310\u003c\/p\u003e \u003cp\u003e12.4 Nonfibrous Stroma and Granulo-Filamentous Deposits 311\u003c\/p\u003e \u003cp\u003e12.5 Connective Tissue Disorders 311\u003c\/p\u003e \u003cp\u003e12.5.1 Ehlers–Danlos Syndrome 311\u003c\/p\u003e \u003cp\u003e12.5.2 Spontaneous Cervical Artery Dissection 317\u003c\/p\u003e \u003cp\u003e12.5.3 Recurrent Preterm Premature Rupture of Fetal Membrane Syndrome 319\u003c\/p\u003e \u003cp\u003eReferences 319\u003c\/p\u003e \u003cp\u003e\u003cb\u003e13 Electron Microscopy in Occupational and Environmental Lung Disease 323\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eVictor L. Roggli\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e13.1 Introduction 323\u003c\/p\u003e \u003cp\u003e13.2 Asbestos 324\u003c\/p\u003e \u003cp\u003e13.2.1 Preparatory Techniques 324\u003c\/p\u003e \u003cp\u003e13.2.2 Analytical Methodology 326\u003c\/p\u003e \u003cp\u003e13.2.3 Asbestos-Related Diseases 326\u003c\/p\u003e \u003cp\u003e13.2.4 Exposure Categories 330\u003c\/p\u003e \u003cp\u003e13.3 Hypersensitivity Pneumonitis and Sarcoidosis 330\u003c\/p\u003e \u003cp\u003e13.3.1 Preparatory Techniques and Analytical Methodology 331\u003c\/p\u003e \u003cp\u003e13.4 Silicosis 331\u003c\/p\u003e \u003cp\u003e13.4.1 Preparatory Techniques and Analytical Methodology 333\u003c\/p\u003e \u003cp\u003e13.5 Silicate Pneumoconiosis 333\u003c\/p\u003e \u003cp\u003e13.5.1 Talc Pneumoconiosis 333\u003c\/p\u003e \u003cp\u003e13.5.2 Kaolin Worker’s Pneumoconiosis 334\u003c\/p\u003e \u003cp\u003e13.5.3 Mica and Feldspar Pneumoconiosis 334\u003c\/p\u003e \u003cp\u003e13.5.4 Mixed Dust Pneumoconiosis 335\u003c\/p\u003e \u003cp\u003e13.5.5 Preparatory Techniques and Analytical Methodology 335\u003c\/p\u003e \u003cp\u003e13.6 Metal-Induced Diseases 335\u003c\/p\u003e \u003cp\u003e13.6.1 Siderosis 336\u003c\/p\u003e \u003cp\u003e13.6.2 Aluminosis 336\u003c\/p\u003e \u003cp\u003e13.6.3 Hard Metal Lung Disease 336\u003c\/p\u003e \u003cp\u003e13.6.4 Berylliosis 337\u003c\/p\u003e \u003cp\u003e13.6.5 Preparatory Techniques and Analytical Methodology 337\u003c\/p\u003e \u003cp\u003e13.7 Rare-Earth Pneumoconiosis 338\u003c\/p\u003e \u003cp\u003e13.8 Miscellaneous Disorders 338\u003c\/p\u003e \u003cp\u003eReferences 339\u003c\/p\u003e \u003cp\u003e\u003cb\u003e14 General Tissue Preparation Methods 341\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJohn W. Stirling\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e14.1 Introduction 341\u003c\/p\u003e \u003cp\u003e14.1.1 Specimens Suitable for Diagnostic TEM 341\u003c\/p\u003e \u003cp\u003e14.2 Tissue Collection and Dissection 342\u003c\/p\u003e \u003cp\u003e14.2.1 Tissue Cut-Up 343\u003c\/p\u003e \u003cp\u003e14.3 Tissue Processing 345\u003c\/p\u003e \u003cp\u003e14.3.1 Fixatives and Fixation 345\u003c\/p\u003e \u003cp\u003e14.3.2 Primary Fixation: Glutaraldehyde 347\u003c\/p\u003e \u003cp\u003e14.3.3 Secondary Fixation (Post-fixation): Osmium Tetroxide 347\u003c\/p\u003e \u003cp\u003e14.3.4 Fixative Vehicles and Wash Buffers 347\u003c\/p\u003e \u003cp\u003e14.3.5 En Bloc Staining with Uranyl Acetate 348\u003c\/p\u003e \u003cp\u003e14.3.6 Dehydrant and Transition Fluids 348\u003c\/p\u003e \u003cp\u003e14.3.7 Resin Infiltration and Embedding Media 349\u003c\/p\u003e \u003cp\u003e14.3.8 Tissue Embedding 352\u003c\/p\u003e \u003cp\u003e14.4 Tissue Sectioning 352\u003c\/p\u003e \u003cp\u003e14.4.1 Ultramicrotomy 352\u003c\/p\u003e \u003cp\u003e14.4.2 Sectioning Technique and Ultramicrotome Setup 355\u003c\/p\u003e \u003cp\u003e14.4.3 Common Sectioning Problems and Artefacts 356\u003c\/p\u003e \u003cp\u003e14.4.4 Section Staining 362\u003c\/p\u003e \u003cp\u003e14.4.5 Section Contamination and Staining Artefacts 363\u003c\/p\u003e \u003cp\u003eProtocol 364\u003c\/p\u003e \u003cp\u003eProcessing Schedules 364\u003c\/p\u003e \u003cp\u003eReferences 379\u003c\/p\u003e \u003cp\u003e\u003cb\u003e15 Ultrastructural Pathology Today – Paradigm Change and the Impact of Microwave Technology and Telemicroscopy 383\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eJosef A. Schroeder\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e15.1 Diagnostic Electron Microscopy and Paradigm Shift in Pathology 383\u003c\/p\u003e \u003cp\u003e15.2 Standardised and Automated Conventional Tissue Processing 385\u003c\/p\u003e \u003cp\u003e15.3 Microwave-Assisted Sample Preparation 390\u003c\/p\u003e \u003cp\u003e15.4 Cyberspace for Telepathology via the Internet 397\u003c\/p\u003e \u003cp\u003e15.5 Conclusions and Future Prospects 400\u003c\/p\u003e \u003cp\u003eAcknowledgements 404\u003c\/p\u003e \u003cp\u003eReferences 404\u003c\/p\u003e \u003cp\u003e\u003cb\u003e16 Electron Microscopy Methods in Virology 409\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eAlan Curry\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e16.1 Biological Safety Precautions 409\u003c\/p\u003e \u003cp\u003e16.2 Collection of Specimens 410\u003c\/p\u003e \u003cp\u003e16.3 Preparation of Faeces, Vomitus or Urine Samples 410\u003c\/p\u003e \u003cp\u003e16.4 Viruses in Skin Lesions 410\u003c\/p\u003e \u003cp\u003e16.5 Reagents and Methods 411\u003c\/p\u003e \u003cp\u003e16.5.1 Negative Stains 411\u003c\/p\u003e \u003cp\u003e16.6 Coated Grids 412\u003c\/p\u003e \u003cp\u003e16.7 Important Elements in the Negative Staining Procedure 412 \u003c\/p\u003e \u003cp\u003e16.8 TEM Examination 413\u003c\/p\u003e \u003cp\u003e16.9 Immunoelectron Microscopy 413\u003c\/p\u003e \u003cp\u003e16.9.1 Immune Clumping 413\u003c\/p\u003e \u003cp\u003e16.9.2 Solid-Phase Immunoelectron Microscopy 413\u003c\/p\u003e \u003cp\u003e16.9.3 Immunogold Labelling 414\u003c\/p\u003e \u003cp\u003e16.9.4 Particle Measurement 414\u003c\/p\u003e \u003cp\u003e16.10 Thin Sectioning of Virus-Infected Cells or Tissues 414\u003c\/p\u003e \u003cp\u003e16.11 Virology Quality Assurance (QA) Procedures 415\u003c\/p\u003e \u003cp\u003e16.11.1 External QA 415\u003c\/p\u003e \u003cp\u003e16.11.2 Internal QA 415\u003c\/p\u003e \u003cp\u003eAcknowledgements 415\u003c\/p\u003e \u003cp\u003eReferences 416\u003c\/p\u003e \u003cp\u003e\u003cb\u003e17 Digital Imaging for Diagnostic Transmission Electron Microscopy 419\u003cbr\u003e \u003c\/b\u003e\u003ci\u003eGary Paul Edwards\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e17.1 Introduction 419\u003c\/p\u003e \u003cp\u003e17.2 Camera History 419\u003c\/p\u003e \u003cp\u003e17.3 The Pixel Dilemma 420\u003c\/p\u003e \u003cp\u003e17.4 Camera Positioning 421\u003c\/p\u003e \u003cp\u003e17.5 Resolution 422\u003c\/p\u003e \u003cp\u003e17.6 Fibre Coupled or Lens Coupled? 423\u003c\/p\u003e \u003cp\u003e17.7 Sensitivity, Noise and Dynamic Range 424\u003c\/p\u003e \u003cp\u003e17.8 CCD Chip Type (Full Frame or Interline) 426\u003c\/p\u003e \u003cp\u003e17.9 Binning and Frame Rate 426\u003c\/p\u003e \u003cp\u003e17.10 Software 427\u003c\/p\u003e \u003cp\u003e17.11 Choosing the Right Camera 428\u003c\/p\u003e \u003cp\u003eReferences 429\u003c\/p\u003e \u003cp\u003e\u003cb\u003e18 Uncertainty of Measurement 431\u003cbr\u003e \u003c\/b\u003e\u003ci\u003ePierre Filion\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e18.1 Introduction 431\u003c\/p\u003e \u003cp\u003e18.2 Purpose 432\u003c\/p\u003e \u003cp\u003e18.2.1 Diagnostic Value 432\u003c\/p\u003e \u003cp\u003e18.2.2 Internal Quality Control 432\u003c\/p\u003e \u003cp\u003e18.2.3 External Quality Control and Accreditation 432\u003c\/p\u003e \u003cp\u003e18.3 Factors That Influence Quantitative Measurements 433\u003c\/p\u003e \u003cp\u003e18.3.1 Sources of Variation 433\u003c\/p\u003e \u003cp\u003e18.3.2 Alteration of the Intrinsic Dimension of the Structure 434\u003c\/p\u003e \u003cp\u003e18.3.3 Variation Due to the Analytical Equipment and Method 436\u003c\/p\u003e \u003cp\u003e18.3.4 Variation Due to Selection Bias 438\u003c\/p\u003e \u003cp\u003e18.3.5 Measurement Using a Digital Camera 439\u003c\/p\u003e \u003cp\u003e18.4 How to Calculate the UM 440\u003c\/p\u003e \u003cp\u003e18.4.1 Steps Required to Analyse and Calculate the UM 440\u003c\/p\u003e \u003cp\u003e18.4.2 Type of Error and Distribution of Measurements 440\u003c\/p\u003e \u003cp\u003e18.4.3 Calculating the UM 442\u003c\/p\u003e \u003cp\u003e18.4.4 Precision of Measurement and Biological Significance 443\u003c\/p\u003e \u003cp\u003e18.4.5 The Electronic Spread Sheet as an Aid to Calculating UM 443\u003c\/p\u003e \u003cp\u003e18.4.6 Reporting the UM 444\u003c\/p\u003e \u003cp\u003e18.5 Worked Examples 444\u003c\/p\u003e \u003cp\u003e18.5.1 Diameter of Fibrils in a Glomerular Deposit 444\u003c\/p\u003e \u003cp\u003e18.5.2 Thickness of the Glomerular Basement Membrane 445\u003c\/p\u003e \u003cp\u003e18.6 Conclusion 446\u003c\/p\u003e \u003cp\u003eReferences 447\u003c\/p\u003e \u003cp\u003eIndex 449\u003c\/p\u003e  \u003cp\u003e“Thus, this book is a “must-have” for all pathology departments, even if they are not equipped with an EM facility, and it is also a solid proof of the current role of electron microscopy in health care.”  (\u003ci\u003eMicroscopy \u0026amp; Microanalysis\u003c\/i\u003e, 1 August 2013)\u003c\/p\u003e  \u003cp\u003e\u003cb\u003eJohn W. Stirling\u003c\/b\u003e, The Centre for Ultrastructural Pathology, Adelaide, Australia \u003c\/p\u003e\u003cp\u003e\u003cb\u003eAlan Curry\u003c\/b\u003e, Manchester Royal Infirmary, Manchester, UK \u003c\/p\u003e\u003cp\u003e\u003cb\u003eBrian P. Eyden\u003c\/b\u003e, Christie NHS Foundation Trust, Manchester, UK   \u003c\/p\u003e\u003cp\u003e\u003cb\u003eDiagnostic Electron Microscopy\u003c\/b\u003e \u003c\/p\u003e\u003cp\u003e\u003cb\u003eDiagnostic Electron Microscopy: A Practical Guide to Interpretation and Technique\u003c\/b\u003e summarises the current interpretational applications of TEM in diagnostic pathology. This concise and accessible volume provides a working guide to the main, or most useful, applications of the technique including practical topics of concern to laboratory scientists, brief guides to traditional tissue and microbiological preparation techniques, microwave processing, digital imaging and measurement uncertainty. \u003c\/p\u003e\u003cp\u003eThe text features both a screening and interpretational guide for TEM diagnostic applications and current TEM diagnostic tissue preparation methods pertinent to all clinical electron microscope units worldwide. Containing high-quality representative images, this up-to-date text includes detailed information on the most important diagnostic applications of transmission electron microscopy as well as instructions for specific tissues and current basic preparative techniques. \u003c\/p\u003e\u003cp\u003eThe book is relevant to trainee pathologists and practising pathologists who are expected to understand and evaluate\/screen tissues by TEM. In addition, technical and scientific staff involved in tissue preparation and diagnostic tissue evaluation\/screening by TEM will find this text useful.\u003c\/p\u003e","brand":"Wiley","offers":[{"title":"Default Title","offer_id":47989057945829,"sku":"NP9781119973997","price":135.95,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9781119973997.jpg?v=1761782620","url":"https:\/\/k12savings.com\/es\/products\/diagnostic-electron-microscopy-isbn-9781119973997","provider":"K12savings","version":"1.0","type":"link"}