{"product_id":"current-protocols-essential-laboratory-techniques-isbn-9780470942413","title":"Current Protocols Essential Laboratory Techniques","description":"The latest title from the acclaimed \u003ci\u003eCurrent Protocols\u003c\/i\u003e series, \u003ci\u003eCurrent Protocols Essential Laboratory Techniques, 2e\u003c\/i\u003e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory.  From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher.  Written by a combination of distinguished investigators and outstanding faculty, \u003ci\u003eCurrent Protocols Essential Laboratory Techniques, 2e\u003c\/i\u003e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.  Foreword  \u003cp\u003ePreface \u003c\/p\u003e \u003cp\u003eContributors List\u003c\/p\u003e \u003cp\u003eConversion Factors\u003c\/p\u003e \u003cp\u003eFlow Chart: Application of Techniques\u003cbr\u003e  \u003ci\u003eEmily Wiley, Claremont Colleges\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eChapter 1 Volume\/Weight Measurement\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 1.1 Volume Measurement\u003cbr\u003e  Andrew Zanella and Tom Davis, Claremont Colleges\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 1.2 Weight Measurement\u003cbr\u003e  Michael Guzy, Ohaus, Corp.\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eChapter 2 Concentration Measurement\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 2.1 Spectrophotometry\u003cbr\u003e  Rob Morris, Ocean Optics, Inc.\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 2.2 Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy\u003cbr\u003e  Sean R. Gallagher, UVP, LLC\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eChapter 3 Reagent Preparation\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 3.1 Theoretical Discussion\u003cbr\u003e  Deb Chakravarti, Keck Graduate Institute\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 3.2 Practical Discussion\u003cbr\u003e  Deb Chakravarti, Keck Graduate Institute\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 3.3 Recipes\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eIn-House\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eChapter 4 Cell Culture Techniques\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 4.1 Aseptic Technique\u003cbr\u003e  Thomas Bykowski and Brian Stevenson, University of Kentucky College of Medicine\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 4.2 Culture of Escherichia coli and Related Bacteria\u003cbr\u003e  Sean P. Riley, Michael E. Woodman, and Brian Stevenson, University of Kentucky College of Medicine\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eChapter 5 Sample Preparation\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 5.1 Centrifugation and Cell Fractionation In-House\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 5.2 Purification and Concentration of Nucleic Acids\u003cbr\u003e  Dennis H. Dowhan, University of Queensland\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eChapter 6 Chromatography\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 6.1 Overview of Chromatography\u003cbr\u003e  Buddhadeb Mallik, Bulbul Chakravarti, and Deb N. Chakravarti, Keck Graduate Institute of Applied Life Sciences\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 6.2 Column Chromatography\u003cbr\u003e  Deb Chakravarti, Keck Graduate Institute\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eChapter 7 Electrophoresis\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 7.1 Overview of Electrophoresis\u003cbr\u003e  Sean R. Gallagherr, UVP, LLC\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 7.2 Agarose Gel Electrophoresis\u003cbr\u003e  Jennifer A. Armstrong, The Claremont Colleges, and Joseph R. Schulz, Occidental College\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 7.3 SDS-PAGE\u003cbr\u003e  Sean Gallagher, UVP, LLC\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 7.4 Staining of Gels\u003cbr\u003e  Sean Gallagher, UVP, LLC\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eChapter 8 Blotting\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 8.1 Overview of Blotting\u003cbr\u003e  Maria Cristina Negritto, Pomona College, and Glenn M. Manthey, City of Hope, Beckman Research Institute\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 8.2 Nucleic Acid Blotting: Southern and Northern\u003cbr\u003e  Laura L. Mays Hoopes, Pomona College\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 8.3 Protein Blotting: Immunoblotting\u003cbr\u003e  Sean Gallagher, UVP, LLC\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 8.4 Labeling DNA and Probe Preparation\u003cbr\u003e  Karl A. Haushalter, Harvey Mudd College\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eChapter 9 Microscopy\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 9.1 Conventional Light Microscopy\u003cbr\u003e  Eric S. Cole, St. Olaf College\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 9.2 Immunofluorescence Microscopy\u003cbr\u003e  David J. Asai, Harvey Mudd College\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eChapter 10 Enzymatic Reactions\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 10.1 Working with Enzymes\u003cbr\u003e  David Skrincosky and Cindy Santangelo, Worthington Biochemical Corp.\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 10.2 Overview of PCR\u003cbr\u003e  Christine D. Kuslich, Molecular Profiling Institute, Buena Chui, GE Healthcare, and Carl T. Yamashiro, Molecular Profiling Institute\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eUnit 10.3 Real-Time PCR\u003cbr\u003e  Dean Fraga, College of Wooster, Tea Meulia, Ohio Agricultural Research and Development Center, Steven Fenster, Ashland University\u003c\/i\u003e\u003c\/p\u003e \u003cp\u003e\u003ci\u003eChapter 11 Bioinformatics Unit 11.1 Using NCBI Blast\u003cbr\u003e  Nicholas A. Stover, Bradley University, and Andre R.O. Cavalcanti, Pomona College\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003e“Wow! This is really a great book for anyone learning about or working in a research laboratory.”  (\u003ci\u003eDoody’s\u003c\/i\u003e, 30 August 2012)\u003c\/p\u003e \u003cp\u003e \u003c\/p\u003e \u003cp\u003e\u003cb\u003eSean R. Gallagher,\u003c\/b\u003e Ph.D. is the Chief Technology Officer and Director of Product Development for UVP, Inc. (Upland, CA), where he oversees applied technology and product development for specialty light source and life science products. He is on the faculty of the Keck Graduate Institute of Applied Life Sciences and Harvey Mudd College (Claremont, CA) and holds eight patents on technology he developed.  He received his doctorate in Botany from the University of California at Riverside and completed a post-doctoral position at Stanford University.\u003c\/p\u003e \u003cp\u003e\u003cb\u003eEmily A. Wiley,\u003c\/b\u003e Ph.D. is an Associate Professor of Biology in the Joint Science Department of the Claremont-McKenna, Scripps, and Pitzer Colleges, Claremont, CA. She received her doctorate in Molecular Genetics from the University of Washington. She is actively involved in graduate and undergraduate teaching courses and is committed to providing proper training to those students. She has an active research program studying chromatin assembly in \u003ci\u003eTetrahymena thermophila.\u003c\/i\u003e\u003c\/p\u003e  \u003cp\u003eProvides students with the solid grounding in biological laboratory techniques needed to pursue any major in the life and health sciences\u003c\/p\u003e \u003cp\u003eBuilding on the success of its American Publishers Award-winning predecessor, Current Protocols Essential Laboratory Techniques, Second Edition gives new researchers the skills and understanding of the fundamental laboratory procedures that are needed to run experiments, solve problems, and become a productive member of the modern life science laboratory.\u003c\/p\u003e \u003cp\u003eAn indispensable tool for readers, this Second Edition includes chapters on cell culture techniques, electrophoresis, microscopy, enzymatic reactions, and bioinformatics. In addition to covering measurement, preparation of reagents, blotting, chromatography, and real-time PCR, Current Protocols Essential Laboratory Techniques also:\u003c\/p\u003e \u003cul\u003e \u003cli\u003e \u003cp\u003eTeaches new investigators how to perform basic laboratory research techniques\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eExplains solution chemistry and preparation\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eDescribes care and use of common equipment such as pH meters, spectrophotometers, centrifuges, and microscopes\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eCovers basic laboratory safety\u003c\/p\u003e \u003c\/li\u003e \u003cli\u003e \u003cp\u003eProvides access to Current Protocols Essential Laboratory Techniques online\u003c\/p\u003e \u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eWritten by distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, Second Edition is a great source for beginning scientists who want to develop the skills for a fruitful career in research.\u003c\/p\u003e","brand":"Current Protocols","offers":[{"title":"Default Title","offer_id":47989016166629,"sku":"NP9780470942413","price":111.25,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1842\/7735\/files\/9780470942413.jpg?v=1761782449","url":"https:\/\/k12savings.com\/es\/products\/current-protocols-essential-laboratory-techniques-isbn-9780470942413","provider":"K12savings","version":"1.0","type":"link"}