Applications of Fluorescence in Immunoassays

Describes the basic prerequisities for a fluorometric immunoassay: the antibody, the immunological technology, the fluorescent probe, and the instrument. Provides a profile of the clinical applications of the various assay technologies. Topics include: background noise, the mechanization and automation of immunoassays, DNA-based specific binding assays, fluorescence immonosensors, multiparameter analyzing systems and more. Antibodies as Analytical Reagents.

Evolution of Immunoassays.

Non-Isotopic Immunoassays.

Photoluminescence Spectroscopy.

Instruments in Fluorometric Immunoassays.

Photoluminescent Probes.

Fluorescence Immunoassays.

Fluorometric Enzyme Immunoassays.

Other Specific Binding Assays Using Fluorescence.

Multiparametric Immunoassays.

References.

Index.

Ilkka A. Hemmila and James D. Winefordner are the authors of Applications of Fluorescence in Immunoassays, published by Wiley.